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The map label for this gene is queA [H]

Identifier: 73542492

GI number: 73542492

Start: 3077203

End: 3078252

Strand: Direct

Name: queA [H]

Synonym: Reut_A2807

Alternate gene names: 73542492

Gene position: 3077203-3078252 (Clockwise)

Preceding gene: 73542487

Following gene: 73542493

Centisome position: 80.84

GC content: 67.05

Gene sequence:

>1050_bases
ATGTTGACGCTGTCCGATTTCGACTTTCCGCTGCCACCCGAACTGATTGCACAGAGCGCGCTGCCCGACCGCAGTGCGAG
CCGGCTGCTCGTGGTCGAGCGCCTCGCGCCAGGCGAGCAGCCCGATGCCATCCGGCTCGTCGACCGCGCGTTCAGCGACA
TCATCGGGTACCTGAAGCCCGAAGATTTGCTGGTCTTCAACGATACCCGCGTGATCAAGGCGCGCTTCTTCGGCCAGAAG
CCGAGCGGCGGCAAGGTCGAGGTGCTGGTCGAGCGCGTGCTCGATACGCACACCGTGCTGGCCCAGGTGCGCGCATCGAA
AACGCCCGCCGAGGGCAGCCTGCTGCACCTCGCCGAAGACGCGTTCGCTGTCACCGTCGGCCCGCGCGTCGACCAGTTCT
TCACGCTGCGCTTTCCCGCGCCCGCGCTGGACCTGATCGAGCAGTACGGCCGCCTGCCGCTGCCGCCGTACATCACGCAC
GATCCCGACGCCTACGACGAAACCCGCTACCAGACCGTTTACGCGCGCAATCCCGGCGCCGTGGCCGCGCCGACGGCCGG
CCTCCATTTCGACGATGCACTGTTCGCGCGGCTCGACGCCGCCGGCGTGCGGCGTGCGTTCCTGACGCTGCATGTCGGCG
CTGGCACCTTCCAGCCGGTACGCACCGAGAACATCGCCGAACACAAGATGCACTCCGAGTGGTACGCGATTTCGCCGGAA
CTGGCCGATGCCGTTCGCGAAACGCGCAAGCGCGGCGGCCGTGTCATTGCGGTGGGCACGACCTCGCTGCGGGCACTGGA
ATCGGCCGCCAAGCCCGACGGCACGCTCGAAGCCGGCAATGGCGACACCGACATCTTCATCACGCCGGGCTACCAGTTCC
GGCTCGTCGATGCCCTGATCACCAACTTCCACCTGCCCAAGTCGACGCTTCTGATGCTGGTCTCGGCGCTGGCCGGCGTG
GAAGCCATCCGCGCCGCGTACCGCCATGCGGTCGAGGCGCGCTATCGTTTCTTCAGCTACGGCGATGCCATGTTGCTGAC
CCGCCAGTAA

Upstream 100 bases:

>100_bases
CGGCGCCTGGCGCCCAAGCCTTACAATATCGGATTCGCCGATTGTACCTATCCCGGTTGCCGCTCCGCGCGGCGCCTTGC
CGGGGCATTGCCGTTTCCCC

Downstream 100 bases:

>100_bases
CGCCTGAAGCCGAACCGAATCCCGCCATGCTCAATTTCGAACTCATCACTACCGACGGCAACGCACGACGCGGCCGGGTC
ACGCTGAACCACGGCGTGGT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MLTLSDFDFPLPPELIAQSALPDRSASRLLVVERLAPGEQPDAIRLVDRAFSDIIGYLKPEDLLVFNDTRVIKARFFGQK
PSGGKVEVLVERVLDTHTVLAQVRASKTPAEGSLLHLAEDAFAVTVGPRVDQFFTLRFPAPALDLIEQYGRLPLPPYITH
DPDAYDETRYQTVYARNPGAVAAPTAGLHFDDALFARLDAAGVRRAFLTLHVGAGTFQPVRTENIAEHKMHSEWYAISPE
LADAVRETRKRGGRVIAVGTTSLRALESAAKPDGTLEAGNGDTDIFITPGYQFRLVDALITNFHLPKSTLLMLVSALAGV
EAIRAAYRHAVEARYRFFSYGDAMLLTRQ

Sequences:

>Translated_349_residues
MLTLSDFDFPLPPELIAQSALPDRSASRLLVVERLAPGEQPDAIRLVDRAFSDIIGYLKPEDLLVFNDTRVIKARFFGQK
PSGGKVEVLVERVLDTHTVLAQVRASKTPAEGSLLHLAEDAFAVTVGPRVDQFFTLRFPAPALDLIEQYGRLPLPPYITH
DPDAYDETRYQTVYARNPGAVAAPTAGLHFDDALFARLDAAGVRRAFLTLHVGAGTFQPVRTENIAEHKMHSEWYAISPE
LADAVRETRKRGGRVIAVGTTSLRALESAAKPDGTLEAGNGDTDIFITPGYQFRLVDALITNFHLPKSTLLMLVSALAGV
EAIRAAYRHAVEARYRFFSYGDAMLLTRQ
>Mature_349_residues
MLTLSDFDFPLPPELIAQSALPDRSASRLLVVERLAPGEQPDAIRLVDRAFSDIIGYLKPEDLLVFNDTRVIKARFFGQK
PSGGKVEVLVERVLDTHTVLAQVRASKTPAEGSLLHLAEDAFAVTVGPRVDQFFTLRFPAPALDLIEQYGRLPLPPYITH
DPDAYDETRYQTVYARNPGAVAAPTAGLHFDDALFARLDAAGVRRAFLTLHVGAGTFQPVRTENIAEHKMHSEWYAISPE
LADAVRETRKRGGRVIAVGTTSLRALESAAKPDGTLEAGNGDTDIFITPGYQFRLVDALITNFHLPKSTLLMLVSALAGV
EAIRAAYRHAVEARYRFFSYGDAMLLTRQ

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=351, Percent_Identity=51.5669515669516, Blast_Score=338, Evalue=2e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38425; Mature: 38425

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLSDFDFPLPPELIAQSALPDRSASRLLVVERLAPGEQPDAIRLVDRAFSDIIGYLKP
CCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
EDLLVFNDTRVIKARFFGQKPSGGKVEVLVERVLDTHTVLAQVRASKTPAEGSLLHLAED
CCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
AFAVTVGPRVDQFFTLRFPAPALDLIEQYGRLPLPPYITHDPDAYDETRYQTVYARNPGA
CEEEEECCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC
VAAPTAGLHFDDALFARLDAAGVRRAFLTLHVGAGTFQPVRTENIAEHKMHSEWYAISPE
CCCCCCCCCCCHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEECHH
LADAVRETRKRGGRVIAVGTTSLRALESAAKPDGTLEAGNGDTDIFITPGYQFRLVDALI
HHHHHHHHHHHCCEEEEEECHHHHHHHHHCCCCCCEECCCCCCEEEECCCCCEEEHHHHH
TNFHLPKSTLLMLVSALAGVEAIRAAYRHAVEARYRFFSYGDAMLLTRQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEECC
>Mature Secondary Structure
MLTLSDFDFPLPPELIAQSALPDRSASRLLVVERLAPGEQPDAIRLVDRAFSDIIGYLKP
CCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
EDLLVFNDTRVIKARFFGQKPSGGKVEVLVERVLDTHTVLAQVRASKTPAEGSLLHLAED
CCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
AFAVTVGPRVDQFFTLRFPAPALDLIEQYGRLPLPPYITHDPDAYDETRYQTVYARNPGA
CEEEEECCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC
VAAPTAGLHFDDALFARLDAAGVRRAFLTLHVGAGTFQPVRTENIAEHKMHSEWYAISPE
CCCCCCCCCCCHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEECHH
LADAVRETRKRGGRVIAVGTTSLRALESAAKPDGTLEAGNGDTDIFITPGYQFRLVDALI
HHHHHHHHHHHCCEEEEEECHHHHHHHHHCCCCCCEECCCCCCEEEECCCCCEEEHHHHH
TNFHLPKSTLLMLVSALAGVEAIRAAYRHAVEARYRFFSYGDAMLLTRQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA