Click here to switch to the map view.

The map label for this gene is tgt [H]

Identifier: 73542493

GI number: 73542493

Start: 3078279

End: 3079409

Strand: Direct

Name: tgt [H]

Synonym: Reut_A2808

Alternate gene names: 73542493

Gene position: 3078279-3079409 (Clockwise)

Preceding gene: 73542492

Following gene: 73542494

Centisome position: 80.87

GC content: 64.37

Gene sequence:

>1131_bases
ATGCTCAATTTCGAACTCATCACTACCGACGGCAACGCACGACGCGGCCGGGTCACGCTGAACCACGGCGTGGTCGAAAC
GCCCATCTTCATGCCGGTCGGCACCTATGGCTCGGTCAAGGCCATGTCGCCGCTGGAACTCAACGAGATCGGCGCGGAGA
TCATCCTGGGCAACACTTTCCACCTGTGGCTGCGCCCGGGGCTGGACGTGGTGAACACGCATGAGGGCCTGCACCGCTTC
ATCGGCTGGGACAAGCCGATCCTGACCGACTCTGGCGGCTTTCAGGTGTTTTCGCTCGGCGACCTGCGCAAGATCACTGA
AGATGGCGTCACGTTCGCGTCGCCCGTCAACGGCGACAAGCTGTTCCTGTCGCCCGAGATTTCGATGCAGATCCAGCGCA
CGCTGAACTCCGACATCGTCATGCAGTTCGATGAATGCACGCCGTACGAGATCGAAGGCCGCCCCGCCACGCATGAAGAG
GCGGCCAAATCGATGCGCATGAGCCTGCGCTGGGCCAAGCGCTCGCGCGACGAGTTCGAGCGGCTGGCCAACCCGAACGC
GCTGTTCGGCATCGTCCAGGGCGGCATGTTCGAGGACCTGCGTGATGAATCGCTGGCGGGCCTGTCCGAACTGGATTTCC
ATGGCTTCGCGATTGGCGGCCTGTCGGTGGGCGAGCCAAAGGAAGACATGATGCGCGTGCTCGAGCACGTGGCGCCGCGC
CTGCCGGCCGACAAGCCGCATTACCTGATGGGCGTCGGCACGCCGGAAGATCTGGTGGCGGGCGTGGCGGCTGGCGTCGA
CATGTTCGATTGCGTGATGCCGACCCGCAACGCGCGCAACGGCTGGCTGTTCACGCGCTACGGCGACGTCAAGATCCGAA
ACGCTGCGCACCGCAATGACCCGCGCCCGCTCGACGAAAGCTGCGCGTGCTACACCTGCCGCAATTTCTCGCGCGCGTAC
CTGCACCATCTGCACCGTGTCGGCGAAATTCTTGGCGCACGCCTGAACACGATTCACAACCTGCACTACTACCTGCAGCT
CATGCGCGAGGTGCGCGAGTCCATCGAGCAGCACCGCTTCAGCGACTTCCGCCGCCAGTTCGCCAGCGACCGCGCGCGCG
GCACGCGCTGA

Upstream 100 bases:

>100_bases
CGTACCGCCATGCGGTCGAGGCGCGCTATCGTTTCTTCAGCTACGGCGATGCCATGTTGCTGACCCGCCAGTAACGCCTG
AAGCCGAACCGAATCCCGCC

Downstream 100 bases:

>100_bases
GCCGCGAACTTTCCGACGCGGCGCGGTCGAATACCGGGCGACGGGTCTGTCGCGCATCTGCGCCAAGTCGCCCGCAACGC
ACCCCGAACAAACCCGGATT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MLNFELITTDGNARRGRVTLNHGVVETPIFMPVGTYGSVKAMSPLELNEIGAEIILGNTFHLWLRPGLDVVNTHEGLHRF
IGWDKPILTDSGGFQVFSLGDLRKITEDGVTFASPVNGDKLFLSPEISMQIQRTLNSDIVMQFDECTPYEIEGRPATHEE
AAKSMRMSLRWAKRSRDEFERLANPNALFGIVQGGMFEDLRDESLAGLSELDFHGFAIGGLSVGEPKEDMMRVLEHVAPR
LPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRYGDVKIRNAAHRNDPRPLDESCACYTCRNFSRAY
LHHLHRVGEILGARLNTIHNLHYYLQLMREVRESIEQHRFSDFRRQFASDRARGTR

Sequences:

>Translated_376_residues
MLNFELITTDGNARRGRVTLNHGVVETPIFMPVGTYGSVKAMSPLELNEIGAEIILGNTFHLWLRPGLDVVNTHEGLHRF
IGWDKPILTDSGGFQVFSLGDLRKITEDGVTFASPVNGDKLFLSPEISMQIQRTLNSDIVMQFDECTPYEIEGRPATHEE
AAKSMRMSLRWAKRSRDEFERLANPNALFGIVQGGMFEDLRDESLAGLSELDFHGFAIGGLSVGEPKEDMMRVLEHVAPR
LPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRYGDVKIRNAAHRNDPRPLDESCACYTCRNFSRAY
LHHLHRVGEILGARLNTIHNLHYYLQLMREVRESIEQHRFSDFRRQFASDRARGTR
>Mature_376_residues
MLNFELITTDGNARRGRVTLNHGVVETPIFMPVGTYGSVKAMSPLELNEIGAEIILGNTFHLWLRPGLDVVNTHEGLHRF
IGWDKPILTDSGGFQVFSLGDLRKITEDGVTFASPVNGDKLFLSPEISMQIQRTLNSDIVMQFDECTPYEIEGRPATHEE
AAKSMRMSLRWAKRSRDEFERLANPNALFGIVQGGMFEDLRDESLAGLSELDFHGFAIGGLSVGEPKEDMMRVLEHVAPR
LPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRYGDVKIRNAAHRNDPRPLDESCACYTCRNFSRAY
LHHLHRVGEILGARLNTIHNLHYYLQLMREVRESIEQHRFSDFRRQFASDRARGTR

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=46.1971830985916, Blast_Score=310, Evalue=2e-84,
Organism=Escherichia coli, GI1786607, Length=372, Percent_Identity=64.5161290322581, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17544680, Length=371, Percent_Identity=40.7008086253369, Blast_Score=283, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI25146502, Length=164, Percent_Identity=29.8780487804878, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19920510, Length=359, Percent_Identity=43.7325905292479, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI21355311, Length=155, Percent_Identity=31.6129032258064, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42471; Mature: 42471

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFELITTDGNARRGRVTLNHGVVETPIFMPVGTYGSVKAMSPLELNEIGAEIILGNTF
CCEEEEEECCCCCCCCEEEECCCEEECCEEEEECCCCCCCCCCCCCHHHCCCEEEECCEE
HLWLRPGLDVVNTHEGLHRFIGWDKPILTDSGGFQVFSLGDLRKITEDGVTFASPVNGDK
EEEECCCCCHHHHHHHHHHHHCCCCCEEECCCCEEEEECCHHHHHHHCCCEEECCCCCCE
LFLSPEISMQIQRTLNSDIVMQFDECTPYEIEGRPATHEEAAKSMRMSLRWAKRSRDEFE
EEECCCHHHHHHHHCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHH
RLANPNALFGIVQGGMFEDLRDESLAGLSELDFHGFAIGGLSVGEPKEDMMRVLEHVAPR
HHCCCCCEEEECCCCHHHHHHHHHHCCCHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHC
LPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRYGDVKIRNAAHRND
CCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEECCEEEECCCCCCC
PRPLDESCACYTCRNFSRAYLHHLHRVGEILGARLNTIHNLHYYLQLMREVRESIEQHRF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDFRRQFASDRARGTR
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLNFELITTDGNARRGRVTLNHGVVETPIFMPVGTYGSVKAMSPLELNEIGAEIILGNTF
CCEEEEEECCCCCCCCEEEECCCEEECCEEEEECCCCCCCCCCCCCHHHCCCEEEECCEE
HLWLRPGLDVVNTHEGLHRFIGWDKPILTDSGGFQVFSLGDLRKITEDGVTFASPVNGDK
EEEECCCCCHHHHHHHHHHHHCCCCCEEECCCCEEEEECCHHHHHHHCCCEEECCCCCCE
LFLSPEISMQIQRTLNSDIVMQFDECTPYEIEGRPATHEEAAKSMRMSLRWAKRSRDEFE
EEECCCHHHHHHHHCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHH
RLANPNALFGIVQGGMFEDLRDESLAGLSELDFHGFAIGGLSVGEPKEDMMRVLEHVAPR
HHCCCCCEEEECCCCHHHHHHHHHHCCCHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHC
LPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRYGDVKIRNAAHRND
CCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEECCEEEECCCCCCC
PRPLDESCACYTCRNFSRAYLHHLHRVGEILGARLNTIHNLHYYLQLMREVRESIEQHRF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDFRRQFASDRARGTR
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA