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The map label for this gene is pcaJ [H]

Identifier: 73541196

GI number: 73541196

Start: 1624446

End: 1625108

Strand: Reverse

Name: pcaJ [H]

Synonym: Reut_A1505

Alternate gene names: 73541196

Gene position: 1625108-1624446 (Counterclockwise)

Preceding gene: 73541197

Following gene: 73541195

Centisome position: 42.69

GC content: 64.86

Gene sequence:

>663_bases
ATGGACCAAGTCGCAAAGAACCTGCTGGCAGCGCGCGTGGCGCGCGATATCCCGAACGGATCGTACGTGAACCTCGGCAT
CGGCCTGCCGACGCTGGTCGCCAATCATCTGCCCCGGCATCGCGAAATCATCCTGCATAGCGAAAACGGCGTGCTGGGCC
AATGGTCGGCCGCCGAGCCGGGGCAAGAAGATCCTGATTTGATCAACGCGGGCAAGGAACCGGTCCGGCTGGAAAAAGGC
GCGGCGTTCTTCCACCATGCGGACGCCTTCGGCATGATGCGCGGCGGCCATCTTGATATCTGCGTGCTTGGCGCATTCCA
GGTGTCCGGGCACGGTGATCTGGCCAACTGGCACACCGGAGACCCCGATGCCATTCCGGCGGTAGGCGGCGCTATGGACC
TCGCGATCGGCGCCAGGCGCGTGTTCGTCATGATGGACCACATGACCCGCGATGGCGCGAGCAAGCTGCGCCGCCAGTGC
AGTTATCCGCTGACCGGGCTGGCCTGCGTCAGCCGGGTGTACACGGAACTCGGCACCTTCGCCATCGGCCCGCAGGGCGT
GGCCGTCATCGAGATCATCGGCGACATGTCGCCACAGGCACTGCAGTCGGTCACGGACGTGGAGCTTGATTTCACCACCC
TGGCGACGGCCGAGCTTGCCTAG

Upstream 100 bases:

>100_bases
GACCCGGAAGCCGTCGTCACGCCCGGTATCTTCGTCAGCCGCGTGGTGCTGCGCGACAGCGCTGCCATGCAGGCCAACCT
CATGCACGCGAGCTGAAATC

Downstream 100 bases:

>100_bases
CCTTCTCTGCAGGGGCCGGCGGATCGTTCCATCCAATGCCCGGTACCGCACCTACAACGAAAAAAGACTCACCTCAGGAG
ACAACCATGTTTGCTTGGTA

Product: butyryl-CoA:acetate CoA transferase

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MDQVAKNLLAARVARDIPNGSYVNLGIGLPTLVANHLPRHREIILHSENGVLGQWSAAEPGQEDPDLINAGKEPVRLEKG
AAFFHHADAFGMMRGGHLDICVLGAFQVSGHGDLANWHTGDPDAIPAVGGAMDLAIGARRVFVMMDHMTRDGASKLRRQC
SYPLTGLACVSRVYTELGTFAIGPQGVAVIEIIGDMSPQALQSVTDVELDFTTLATAELA

Sequences:

>Translated_220_residues
MDQVAKNLLAARVARDIPNGSYVNLGIGLPTLVANHLPRHREIILHSENGVLGQWSAAEPGQEDPDLINAGKEPVRLEKG
AAFFHHADAFGMMRGGHLDICVLGAFQVSGHGDLANWHTGDPDAIPAVGGAMDLAIGARRVFVMMDHMTRDGASKLRRQC
SYPLTGLACVSRVYTELGTFAIGPQGVAVIEIIGDMSPQALQSVTDVELDFTTLATAELA
>Mature_220_residues
MDQVAKNLLAARVARDIPNGSYVNLGIGLPTLVANHLPRHREIILHSENGVLGQWSAAEPGQEDPDLINAGKEPVRLEKG
AAFFHHADAFGMMRGGHLDICVLGAFQVSGHGDLANWHTGDPDAIPAVGGAMDLAIGARRVFVMMDHMTRDGASKLRRQC
SYPLTGLACVSRVYTELGTFAIGPQGVAVIEIIGDMSPQALQSVTDVELDFTTLATAELA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=206, Percent_Identity=39.8058252427184, Blast_Score=151, Evalue=4e-37,
Organism=Homo sapiens, GI11545841, Length=182, Percent_Identity=42.3076923076923, Blast_Score=148, Evalue=4e-36,
Organism=Escherichia coli, GI1788552, Length=176, Percent_Identity=46.5909090909091, Blast_Score=157, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17531517, Length=211, Percent_Identity=41.2322274881517, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21358325, Length=192, Percent_Identity=43.2291666666667, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24655794, Length=192, Percent_Identity=43.2291666666667, Blast_Score=159, Evalue=1e-39,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 23416; Mature: 23416

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQVAKNLLAARVARDIPNGSYVNLGIGLPTLVANHLPRHREIILHSENGVLGQWSAAEP
CCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCCEECCCCCCC
GQEDPDLINAGKEPVRLEKGAAFFHHADAFGMMRGGHLDICVLGAFQVSGHGDLANWHTG
CCCCCHHHHCCCCCEEECCCCCEEEHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCC
DPDAIPAVGGAMDLAIGARRVFVMMDHMTRDGASKLRRQCSYPLTGLACVSRVYTELGTF
CCCCCCCCCCHHHHHHHHHHHEEHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCE
AIGPQGVAVIEIIGDMSPQALQSVTDVELDFTTLATAELA
EECCCCEEEEEEECCCCHHHHHHHHCCEECHHHHHHECCC
>Mature Secondary Structure
MDQVAKNLLAARVARDIPNGSYVNLGIGLPTLVANHLPRHREIILHSENGVLGQWSAAEP
CCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCCEECCCCCCC
GQEDPDLINAGKEPVRLEKGAAFFHHADAFGMMRGGHLDICVLGAFQVSGHGDLANWHTG
CCCCCHHHHCCCCCEEECCCCCEEEHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCC
DPDAIPAVGGAMDLAIGARRVFVMMDHMTRDGASKLRRQCSYPLTGLACVSRVYTELGTF
CCCCCCCCCCHHHHHHHHHHHEEHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCE
AIGPQGVAVIEIIGDMSPQALQSVTDVELDFTTLATAELA
EECCCCEEEEEEECCCCHHHHHHHHCCEECHHHHHHECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]