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The map label for this gene is tig [H]

Identifier: 73541073

GI number: 73541073

Start: 1486278

End: 1487633

Strand: Direct

Name: tig [H]

Synonym: Reut_A1379

Alternate gene names: 73541073

Gene position: 1486278-1487633 (Clockwise)

Preceding gene: 161611262

Following gene: 73541074

Centisome position: 39.05

GC content: 61.73

Gene sequence:

>1356_bases
ATGTCGAACGTCATTGAAAACCTCGGCAAACTGGACCGCAAGGTGACCCTGGCCATTCCCAAGGCCGAAGTCGAAAAGGA
AAAGCAGGACCGCCTGGTTCGCCTGTCGAAGACGGTGAAGATGTCGGGCTTCCGTCCGGGCAAGGTGCCGATGAAGATGG
TCGAGAAGCAATACGGCCAGCAAGTCGAATTCGAAGTGCGCTTCGACAAAGCTGCCCGCAAGTTCTTCGACATCACCAAG
GAACAGGACGTCAAGGTTGCTGGCCAGCCGAAGTTCGAGATCAAGACCGAAGGCGTGGGCGAGGACGAAGTCGCGTTCGA
CGCCACCTTCGAGGTGTACCCGGAAGTCAAGCTGGGCGACCTGACTGGCGCGGAAGTGACCCGCACCAAGACCGAGATCA
CCGATGCCGAGATCGACAAGACCATCGACATCCTGCGCAAACAGCGCGTGCACTACCACGTGCGCGGCGAAGCCGGCGAG
CACGGTGACGGCGGCGGCGATGTGACCGCCCAGAACGGCGACCGCGTGACGCTGGACTTCGTCGGCAAGATCGAAGGCGT
GGAATTCGCTGGCGGCAAGGCTGAGGACTTCCCGTTCGTGCTCGGCGAAGGCCGCATGCTGCCCGAGTTCGAAGCTGCCA
CGCTGGGCCTGAAGGTCGGTGAGAGCAAGAGCTTCCCGCTGTCGTTCCCGGCCGACTATCACGGCAAGGACGTGGCCGGC
AAGACCGCCGAGTTCACCGTCACGCTGAAGAAGATCGAGTGGGCGCACCTGCCGGAAGTGAACGACGCGTTCGCGAAGTC
GCTGGGCATCGCCGACGGCAACGTCGACAAGATGCGTGCTGATATCCGCGAAAACCTCGAGCGCGAAGTGAAGCGCCGCA
CGCACGCCATGCTGAAGGATCAGGTCATGGAAGCGCTGCTGAAGGTGAGTGAGCTGGATGTGCCGAAGGCCCTGATCGAA
CAGGACCAGGAGCGCCTGGTGGAAATGGCCCGCCGCGACCTCGAGCAGCGTGGCATGCCGAACGCCAAAGACATGCCGAT
TCCGGCCGAGATGTTCGCACAGCAGGCCGAGCGCCGCGTCAAGCTGGGCCTGATCCTGGCCGAGATCGTCAAGGCCCACG
GCCTGGAAGCCAAGGCTGACCAGATCAAGGCCGAGATCGAGGACTTCGCCAAGAGCTACGAAGATCCGAAGGAAGTCATG
CGCTGGTACTACGGCGACCAGCAGCGCCTGGCCGAGATGGAAGCCTACGTGCTCGAAAACAACGTGGTAAATTTCGTGTG
CGACAAGGCCAAGGTCACCGACAAGTCGGTGTCCTTCGAGGAACTGACCGCCGAAGGCAACGCACAGCAAGCCTGA

Upstream 100 bases:

>100_bases
GCGGAGCTTGCAATTCGCGGGCACGCGTCAGTGCCGGACTGGCCGGCGCACAAGCGATTGCGCTACGAACACAACTACAT
TTCTGGATTGGATCACGACC

Downstream 100 bases:

>100_bases
GGTCCGGCATGATCCGCAGTCCCCGCGCCGTAGTGCGGGGGCGTGAAGCCCGGCGTTCCTTGCCATCCAACGATACCCTG
GAGAACTTGCATGACCCGCA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MSNVIENLGKLDRKVTLAIPKAEVEKEKQDRLVRLSKTVKMSGFRPGKVPMKMVEKQYGQQVEFEVRFDKAARKFFDITK
EQDVKVAGQPKFEIKTEGVGEDEVAFDATFEVYPEVKLGDLTGAEVTRTKTEITDAEIDKTIDILRKQRVHYHVRGEAGE
HGDGGGDVTAQNGDRVTLDFVGKIEGVEFAGGKAEDFPFVLGEGRMLPEFEAATLGLKVGESKSFPLSFPADYHGKDVAG
KTAEFTVTLKKIEWAHLPEVNDAFAKSLGIADGNVDKMRADIRENLEREVKRRTHAMLKDQVMEALLKVSELDVPKALIE
QDQERLVEMARRDLEQRGMPNAKDMPIPAEMFAQQAERRVKLGLILAEIVKAHGLEAKADQIKAEIEDFAKSYEDPKEVM
RWYYGDQQRLAEMEAYVLENNVVNFVCDKAKVTDKSVSFEELTAEGNAQQA

Sequences:

>Translated_451_residues
MSNVIENLGKLDRKVTLAIPKAEVEKEKQDRLVRLSKTVKMSGFRPGKVPMKMVEKQYGQQVEFEVRFDKAARKFFDITK
EQDVKVAGQPKFEIKTEGVGEDEVAFDATFEVYPEVKLGDLTGAEVTRTKTEITDAEIDKTIDILRKQRVHYHVRGEAGE
HGDGGGDVTAQNGDRVTLDFVGKIEGVEFAGGKAEDFPFVLGEGRMLPEFEAATLGLKVGESKSFPLSFPADYHGKDVAG
KTAEFTVTLKKIEWAHLPEVNDAFAKSLGIADGNVDKMRADIRENLEREVKRRTHAMLKDQVMEALLKVSELDVPKALIE
QDQERLVEMARRDLEQRGMPNAKDMPIPAEMFAQQAERRVKLGLILAEIVKAHGLEAKADQIKAEIEDFAKSYEDPKEVM
RWYYGDQQRLAEMEAYVLENNVVNFVCDKAKVTDKSVSFEELTAEGNAQQA
>Mature_450_residues
SNVIENLGKLDRKVTLAIPKAEVEKEKQDRLVRLSKTVKMSGFRPGKVPMKMVEKQYGQQVEFEVRFDKAARKFFDITKE
QDVKVAGQPKFEIKTEGVGEDEVAFDATFEVYPEVKLGDLTGAEVTRTKTEITDAEIDKTIDILRKQRVHYHVRGEAGEH
GDGGGDVTAQNGDRVTLDFVGKIEGVEFAGGKAEDFPFVLGEGRMLPEFEAATLGLKVGESKSFPLSFPADYHGKDVAGK
TAEFTVTLKKIEWAHLPEVNDAFAKSLGIADGNVDKMRADIRENLEREVKRRTHAMLKDQVMEALLKVSELDVPKALIEQ
DQERLVEMARRDLEQRGMPNAKDMPIPAEMFAQQAERRVKLGLILAEIVKAHGLEAKADQIKAEIEDFAKSYEDPKEVMR
WYYGDQQRLAEMEAYVLENNVVNFVCDKAKVTDKSVSFEELTAEGNAQQA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=443, Percent_Identity=38.8261851015801, Blast_Score=311, Evalue=8e-86,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 50630; Mature: 50499

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVIENLGKLDRKVTLAIPKAEVEKEKQDRLVRLSKTVKMSGFRPGKVPMKMVEKQYGQ
CCHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
QVEFEVRFDKAARKFFDITKEQDVKVAGQPKFEIKTEGVGEDEVAFDATFEVYPEVKLGD
CEEEEEEHHHHHHHHHHCCCCCCCEECCCCCEEEEECCCCCCCEEEEEEEEECCCEEECC
LTGAEVTRTKTEITDAEIDKTIDILRKQRVHYHVRGEAGEHGDGGGDVTAQNGDRVTLDF
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEECCCCEEEEEE
VGKIEGVEFAGGKAEDFPFVLGEGRMLPEFEAATLGLKVGESKSFPLSFPADYHGKDVAG
ECCCCCEEECCCCCCCCCEEECCCCCCCCHHHHHEEEEECCCCCCCCCCCCCCCCCCCCC
KTAEFTVTLKKIEWAHLPEVNDAFAKSLGIADGNVDKMRADIRENLEREVKRRTHAMLKD
CCEEEEEEEEEECHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QVMEALLKVSELDVPKALIEQDQERLVEMARRDLEQRGMPNAKDMPIPAEMFAQQAERRV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
KLGLILAEIVKAHGLEAKADQIKAEIEDFAKSYEDPKEVMRWYYGDQQRLAEMEAYVLEN
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHC
NVVNFVCDKAKVTDKSVSFEELTAEGNAQQA
CCEEEEECCCCCCCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure 
SNVIENLGKLDRKVTLAIPKAEVEKEKQDRLVRLSKTVKMSGFRPGKVPMKMVEKQYGQ
CHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
QVEFEVRFDKAARKFFDITKEQDVKVAGQPKFEIKTEGVGEDEVAFDATFEVYPEVKLGD
CEEEEEEHHHHHHHHHHCCCCCCCEECCCCCEEEEECCCCCCCEEEEEEEEECCCEEECC
LTGAEVTRTKTEITDAEIDKTIDILRKQRVHYHVRGEAGEHGDGGGDVTAQNGDRVTLDF
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEECCCCEEEEEE
VGKIEGVEFAGGKAEDFPFVLGEGRMLPEFEAATLGLKVGESKSFPLSFPADYHGKDVAG
ECCCCCEEECCCCCCCCCEEECCCCCCCCHHHHHEEEEECCCCCCCCCCCCCCCCCCCCC
KTAEFTVTLKKIEWAHLPEVNDAFAKSLGIADGNVDKMRADIRENLEREVKRRTHAMLKD
CCEEEEEEEEEECHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QVMEALLKVSELDVPKALIEQDQERLVEMARRDLEQRGMPNAKDMPIPAEMFAQQAERRV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
KLGLILAEIVKAHGLEAKADQIKAEIEDFAKSYEDPKEVMRWYYGDQQRLAEMEAYVLEN
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHC
NVVNFVCDKAKVTDKSVSFEELTAEGNAQQA
CCEEEEECCCCCCCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA