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The map label for this gene is mnmC

Identifier: 161611262

GI number: 161611262

Start: 1484119

End: 1486125

Strand: Direct

Name: mnmC

Synonym: Reut_A1378

Alternate gene names: 161611262

Gene position: 1484119-1486125 (Clockwise)

Preceding gene: 73541070

Following gene: 73541073

Centisome position: 38.99

GC content: 67.36

Gene sequence:

>2007_bases
ATGTCGCGCGCACTCGAGCCCGCCGAGCCTATTCTGTCTGCCGAAGGCACTCCTTATTCTCCACGCTACGACGACGTCTA
CCACAGCACGGAAGGCGGCCTGGCGCAGGCGCATTACGTATTCCTGGGTGGCAATGGATTGCCGCAAGCCTGGGCGGGGC
GCGATCAGTTCGTGATCGTCGAGACCGGATTCGGGCAAGGGCTGAACTTCCTGGCGACGTGGCAGGCGTGGCGTAACGAT
CCGCAGCGCTGTCGCCGCCTGCACTTCGTCTCGATCGAAAAACATCCGTTCACGCGCGAGGGACTGGCGCAACTGCATGC
CGGGCTTGCGGATATGTTGCCGCTCGCGCAGCAACTGCAAGCGGCCTGGCCGGATGCGCTGCCCGGCCTGCATCGGCTCG
CGTTCGAGGATGGCGGAGTGACGCTGACACTGGCCCTCGGAGATATCGAGGCAGTGCTGCCCAAGCTGGTGGCCGGCGCG
GATGCGTTCTACCTCGATGGCTTTTCTCCGTCGCGCAATGCGGACATGTGGTCGCCGTTGGTGTTTCGCGGCCTTGCGCG
GCTCGCACGGCCGGGAGCCACGCTGGCGACCTATACGGCGGCGGGCTTCGTGCGCCGGGGTCTGCGGGAGGCGGGTTTCG
ACGCGCGCAAGGCGCCGGGCTTCGGTGGCAAGCGGGACATGACGGTTGCGACGTTTTGCCCGGTATGGAAGTCGCGGCGG
CATGCGCCGACTCCTGCGCCGGAATGGAAGGTGCGACACGCTATCGTGATTGGAGCTGGGCTTGCGGGCTGTGCCGTGAC
GGAACGCCTGGCCTCGCGTGGCTGGCAGGTGACGTTGTTCGACGTACATGACGGCCCGGCGCGGCAAACGTCGTCCCATC
GTGCGGCTGCGATGCATGCGCATATCTCGTCCGACGACAGCCTGCTGTCCCGGCTGTCGCGCGCTGGCAACCAATACGCG
CTGCGTGCCTGGGCCGCGCTTGCTGGTGCGGGTCAACCGGTGGGGTGGCATGGCTGCGGCGTGCTGCAGATCGGCGAGAA
CGAAGCAGAAGCCGAGGCGCAGCGCGCTGCGCTCGAAGCACTCGGTCTGCCGGAGTCCTTCGTGCGCTGGATGTCGCCGG
GGGAGGCGGCATCGCAACACGGCGCCGGCGTGCCGCGCGGAGGGCTGTGGTTTCCGCAGGGCGGCTGGGTTGCGCCGCCC
GATATCTGCAGTGCTCAGTTGCGCCGCGCGGGCGATGCCGTGCAGTCGAGGTTTGGTACGCGAGTGGAGCGCATTCGCCG
CCGCGACGACGAATGGGAAGCCATCGACGAAGATGGTGCCGTCCTGGCTTCGGCGCCCATTCTTGTGCTGGCCAACGCTC
ACGAAGCGCCGCGGCTCGCGCCGATGCGGCACCAGGTGATGCGACGCGTGCGCGGCCAGCTCACCACGCTCGCGCCTGGG
CAAGTGACAGCGCTCGGATCGTGGCCGGATTGCGTGGTGACCGGTGCGGGATACCTGCTGCCGGTGTCGGAAGATGGCAG
CGCGCGGGTGGGTTCGAGCTATGAAGCCGACGATGGACCGCTGGTCGCGCGAACCGAAGTGCATGCGGCAAACCTTGATC
GCCTGGCGGGGCTGTTGCCGCAGGCTGCTGCAGCTGCCGGCAATCTCGATGCCGCGGCGATGAGCGGATATGTCGGCGTG
CGCACAGTGTCACACAACCGCTTGCCGCTGATTGGCCAGATGCCGGACGAGGCGGCGGCGATAGCGGCGGCTCCGTCACT
GCGCGGAGCGCACTTGCGCGACATTCCAAGGGTGCCAGGGCTTTACGCAGCGCTTGCCTACGCGTCACGCGGCCTCACAT
GGGCCGCATTGGGCGCGGAGTTGCTGGCAAGCCAGATCGAAGGCGACCCACTGCCGCTCGAAGCCGATCTGGTAGACGCC
ATCGACCCGGCGCGCCTGCTGCTGCGTGCACTGCGTCATGGGCAGGTTGGCCGTGAAGAAGGGCATTCCAGCGGACTATC
AGGGTAA

Upstream 100 bases:

>100_bases
GCGGCGGCCGGTGCGGCCGGGCCGGATTGCAGGACGGGGCGCTATGCTACCATCCGCCGCCAGTCGCCCCCTGACTCGCG
TTCCCGTTTCCTGCCCATCC

Downstream 100 bases:

>100_bases
AAACTGCCGGAACCGGACGCAAACCGTGCGATAATGCTCGTTTTCCGTTTGCGCGGAGCTTGCAATTCGCGGGCACGCGT
CAGTGCCGGACTGGCCGGCG

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MSRALEPAEPILSAEGTPYSPRYDDVYHSTEGGLAQAHYVFLGGNGLPQAWAGRDQFVIVETGFGQGLNFLATWQAWRND
PQRCRRLHFVSIEKHPFTREGLAQLHAGLADMLPLAQQLQAAWPDALPGLHRLAFEDGGVTLTLALGDIEAVLPKLVAGA
DAFYLDGFSPSRNADMWSPLVFRGLARLARPGATLATYTAAGFVRRGLREAGFDARKAPGFGGKRDMTVATFCPVWKSRR
HAPTPAPEWKVRHAIVIGAGLAGCAVTERLASRGWQVTLFDVHDGPARQTSSHRAAAMHAHISSDDSLLSRLSRAGNQYA
LRAWAALAGAGQPVGWHGCGVLQIGENEAEAEAQRAALEALGLPESFVRWMSPGEAASQHGAGVPRGGLWFPQGGWVAPP
DICSAQLRRAGDAVQSRFGTRVERIRRRDDEWEAIDEDGAVLASAPILVLANAHEAPRLAPMRHQVMRRVRGQLTTLAPG
QVTALGSWPDCVVTGAGYLLPVSEDGSARVGSSYEADDGPLVARTEVHAANLDRLAGLLPQAAAAAGNLDAAAMSGYVGV
RTVSHNRLPLIGQMPDEAAAIAAAPSLRGAHLRDIPRVPGLYAALAYASRGLTWAALGAELLASQIEGDPLPLEADLVDA
IDPARLLLRALRHGQVGREEGHSSGLSG

Sequences:

>Translated_668_residues
MSRALEPAEPILSAEGTPYSPRYDDVYHSTEGGLAQAHYVFLGGNGLPQAWAGRDQFVIVETGFGQGLNFLATWQAWRND
PQRCRRLHFVSIEKHPFTREGLAQLHAGLADMLPLAQQLQAAWPDALPGLHRLAFEDGGVTLTLALGDIEAVLPKLVAGA
DAFYLDGFSPSRNADMWSPLVFRGLARLARPGATLATYTAAGFVRRGLREAGFDARKAPGFGGKRDMTVATFCPVWKSRR
HAPTPAPEWKVRHAIVIGAGLAGCAVTERLASRGWQVTLFDVHDGPARQTSSHRAAAMHAHISSDDSLLSRLSRAGNQYA
LRAWAALAGAGQPVGWHGCGVLQIGENEAEAEAQRAALEALGLPESFVRWMSPGEAASQHGAGVPRGGLWFPQGGWVAPP
DICSAQLRRAGDAVQSRFGTRVERIRRRDDEWEAIDEDGAVLASAPILVLANAHEAPRLAPMRHQVMRRVRGQLTTLAPG
QVTALGSWPDCVVTGAGYLLPVSEDGSARVGSSYEADDGPLVARTEVHAANLDRLAGLLPQAAAAAGNLDAAAMSGYVGV
RTVSHNRLPLIGQMPDEAAAIAAAPSLRGAHLRDIPRVPGLYAALAYASRGLTWAALGAELLASQIEGDPLPLEADLVDA
IDPARLLLRALRHGQVGREEGHSSGLSG
>Mature_667_residues
SRALEPAEPILSAEGTPYSPRYDDVYHSTEGGLAQAHYVFLGGNGLPQAWAGRDQFVIVETGFGQGLNFLATWQAWRNDP
QRCRRLHFVSIEKHPFTREGLAQLHAGLADMLPLAQQLQAAWPDALPGLHRLAFEDGGVTLTLALGDIEAVLPKLVAGAD
AFYLDGFSPSRNADMWSPLVFRGLARLARPGATLATYTAAGFVRRGLREAGFDARKAPGFGGKRDMTVATFCPVWKSRRH
APTPAPEWKVRHAIVIGAGLAGCAVTERLASRGWQVTLFDVHDGPARQTSSHRAAAMHAHISSDDSLLSRLSRAGNQYAL
RAWAALAGAGQPVGWHGCGVLQIGENEAEAEAQRAALEALGLPESFVRWMSPGEAASQHGAGVPRGGLWFPQGGWVAPPD
ICSAQLRRAGDAVQSRFGTRVERIRRRDDEWEAIDEDGAVLASAPILVLANAHEAPRLAPMRHQVMRRVRGQLTTLAPGQ
VTALGSWPDCVVTGAGYLLPVSEDGSARVGSSYEADDGPLVARTEVHAANLDRLAGLLPQAAAAAGNLDAAAMSGYVGVR
TVSHNRLPLIGQMPDEAAAIAAAPSLRGAHLRDIPRVPGLYAALAYASRGLTWAALGAELLASQIEGDPLPLEADLVDAI
DPARLLLRALRHGQVGREEGHSSGLSG

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=677, Percent_Identity=35.3028064992614, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_CUPPJ (Q472D5)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_295592.2
- GeneID:   3611169
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A1378
- NMPDR:   fig|264198.3.peg.2132
- HOGENOM:   HBG677640
- ProtClustDB:   PRK01747
- BioCyc:   REUT264198:REUT_A1378-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 71045; Mature: 70914

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRALEPAEPILSAEGTPYSPRYDDVYHSTEGGLAQAHYVFLGGNGLPQAWAGRDQFVIV
CCCCCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEE
ETGFGQGLNFLATWQAWRNDPQRCRRLHFVSIEKHPFTREGLAQLHAGLADMLPLAQQLQ
ECCCCCCCCHHHHHHHHCCCHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAWPDALPGLHRLAFEDGGVTLTLALGDIEAVLPKLVAGADAFYLDGFSPSRNADMWSPL
HHCCHHCCCHHHHEECCCCEEEEEEECCHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHH
VFRGLARLARPGATLATYTAAGFVRRGLREAGFDARKAPGFGGKRDMTVATFCPVWKSRR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECHHHHCCC
HAPTPAPEWKVRHAIVIGAGLAGCAVTERLASRGWQVTLFDVHDGPARQTSSHRAAAMHA
CCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHEE
HISSDDSLLSRLSRAGNQYALRAWAALAGAGQPVGWHGCGVLQIGENEAEAEAQRAALEA
ECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
LGLPESFVRWMSPGEAASQHGAGVPRGGLWFPQGGWVAPPDICSAQLRRAGDAVQSRFGT
HCCCHHHHHHCCCCHHHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RVERIRRRDDEWEAIDEDGAVLASAPILVLANAHEAPRLAPMRHQVMRRVRGQLTTLAPG
HHHHHHCCCCCHHHCCCCCCEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHCEEECCCC
QVTALGSWPDCVVTGAGYLLPVSEDGSARVGSSYEADDGPLVARTEVHAANLDRLAGLLP
CEEEECCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
QAAAAAGNLDAAAMSGYVGVRTVSHNRLPLIGQMPDEAAAIAAAPSLRGAHLRDIPRVPG
HHHHHCCCCCHHHHCCCEEEEEECCCCCCEECCCCCHHHHHHCCCCCCCCCHHCCCCCCH
LYAALAYASRGLTWAALGAELLASQIEGDPLPLEADLVDAIDPARLLLRALRHGQVGREE
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCC
GHSSGLSG
CCCCCCCC
>Mature Secondary Structure 
SRALEPAEPILSAEGTPYSPRYDDVYHSTEGGLAQAHYVFLGGNGLPQAWAGRDQFVIV
CCCCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEE
ETGFGQGLNFLATWQAWRNDPQRCRRLHFVSIEKHPFTREGLAQLHAGLADMLPLAQQLQ
ECCCCCCCCHHHHHHHHCCCHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAWPDALPGLHRLAFEDGGVTLTLALGDIEAVLPKLVAGADAFYLDGFSPSRNADMWSPL
HHCCHHCCCHHHHEECCCCEEEEEEECCHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHH
VFRGLARLARPGATLATYTAAGFVRRGLREAGFDARKAPGFGGKRDMTVATFCPVWKSRR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECHHHHCCC
HAPTPAPEWKVRHAIVIGAGLAGCAVTERLASRGWQVTLFDVHDGPARQTSSHRAAAMHA
CCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHEE
HISSDDSLLSRLSRAGNQYALRAWAALAGAGQPVGWHGCGVLQIGENEAEAEAQRAALEA
ECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
LGLPESFVRWMSPGEAASQHGAGVPRGGLWFPQGGWVAPPDICSAQLRRAGDAVQSRFGT
HCCCHHHHHHCCCCHHHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RVERIRRRDDEWEAIDEDGAVLASAPILVLANAHEAPRLAPMRHQVMRRVRGQLTTLAPG
HHHHHHCCCCCHHHCCCCCCEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHCEEECCCC
QVTALGSWPDCVVTGAGYLLPVSEDGSARVGSSYEADDGPLVARTEVHAANLDRLAGLLP
CEEEECCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
QAAAAAGNLDAAAMSGYVGVRTVSHNRLPLIGQMPDEAAAIAAAPSLRGAHLRDIPRVPG
HHHHHCCCCCHHHHCCCEEEEEECCCCCCEECCCCCHHHHHHCCCCCCCCCHHCCCCCCH
LYAALAYASRGLTWAALGAELLASQIEGDPLPLEADLVDAIDPARLLLRALRHGQVGREE
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCC
GHSSGLSG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA