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The map label for this gene is rfbD [H]

Identifier: 73540419

GI number: 73540419

Start: 772780

End: 773694

Strand: Direct

Name: rfbD [H]

Synonym: Reut_A0713

Alternate gene names: 73540419

Gene position: 772780-773694 (Clockwise)

Preceding gene: 73540418

Following gene: 73540420

Centisome position: 20.3

GC content: 61.09

Gene sequence:

>915_bases
ATGCAGCGTAAGCGTACGGTGCCGACTCTACTCGTAACAGGATGCAACGGCCAGGTCGGATTCGAACTGCGTCGCAGCCT
GGCCCCGCTGGGCAATGTGGTCGCGCTGGATCGCTCCGCCTGTGACCTGGCAAATCCCGATGCGCTGCGCCGCGTGGTGC
GCCAGTGCCGGCCCGACGTCATCGTCAATCCGGCGGCCTATACGGCTGTAGATAAAGCGGAAACCGATGCAGAGGTCGCA
TTCGCCATCAATGCCACTGCGGCCGGTATCCTGGCCGAAGAGGCGAAGGCCCTTGGCAGCCTTCTGGTGCACTATTCGAG
CGACTACGTATTCGACGGCACCAAGGACGGCGCTTACGTCGAGACCGATGCGGTCAATCCGCAATCCGTCTACGGTAAAA
GCAAGCTCGCAGGCGAACAGGCCATTGCCGCTACAGGCGTCCAGTCCGTTGTGCTGCGCACGTGCTGGGTAGCCGGTGCG
CATGGCGGCAACTTTGCAAAGACCATGCTGCGCCTTGGCAAGGAGCGCGATAGCTTGCGTGTCATCGCCGACCAGTTTGG
CGCCCCGACGACCGCATCGCTGATTGCAGATGTCACCGCTCAGATTGTGGCTCGTCACTGGCTGGCGGGCGATCGTCAGG
CGTTTGCCAACGGCATTTATCACCTTGCAGCAGCGGGCGAGACGACCTGGCACGGCTACGCCACCGAAGTTCTTCGTTAT
GCGAAAGCGCATGGTGTGGAACTCAAGGCCGATCCTGAGCAAATCGAAGCCATCCCTGCAACGGCATATCCGCTTCCGGC
ACCGCGTCCGTCCAATTCGAGACTGGATACCACGCGCCTGCAGGAAACCTTCGGCATCCATCTACCGGACTGGAAGCAGG
GCGTGCAGTTCCTGCTCGATCAGATTCTTCCCTAA

Upstream 100 bases:

>100_bases
GAGACGGGGCTGCGCAAGACGGTGCAGTGGTATCTCGACAACCAGGCGTGGGTGCAGGACGTGATGTCGGGCGAGTATCG
GAACTGGGTGGCGAAACAAT

Downstream 100 bases:

>100_bases
GAGTGGTCAATATGCGCAAAGGCATCATCCTGGCCGGGGGTTCCGGCACGCGGCTCTATCCCATCACGCGGTCTGTATCG
AAGCAGCTGCTGCCGGTATA

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MQRKRTVPTLLVTGCNGQVGFELRRSLAPLGNVVALDRSACDLANPDALRRVVRQCRPDVIVNPAAYTAVDKAETDAEVA
FAINATAAGILAEEAKALGSLLVHYSSDYVFDGTKDGAYVETDAVNPQSVYGKSKLAGEQAIAATGVQSVVLRTCWVAGA
HGGNFAKTMLRLGKERDSLRVIADQFGAPTTASLIADVTAQIVARHWLAGDRQAFANGIYHLAAAGETTWHGYATEVLRY
AKAHGVELKADPEQIEAIPATAYPLPAPRPSNSRLDTTRLQETFGIHLPDWKQGVQFLLDQILP

Sequences:

>Translated_304_residues
MQRKRTVPTLLVTGCNGQVGFELRRSLAPLGNVVALDRSACDLANPDALRRVVRQCRPDVIVNPAAYTAVDKAETDAEVA
FAINATAAGILAEEAKALGSLLVHYSSDYVFDGTKDGAYVETDAVNPQSVYGKSKLAGEQAIAATGVQSVVLRTCWVAGA
HGGNFAKTMLRLGKERDSLRVIADQFGAPTTASLIADVTAQIVARHWLAGDRQAFANGIYHLAAAGETTWHGYATEVLRY
AKAHGVELKADPEQIEAIPATAYPLPAPRPSNSRLDTTRLQETFGIHLPDWKQGVQFLLDQILP
>Mature_304_residues
MQRKRTVPTLLVTGCNGQVGFELRRSLAPLGNVVALDRSACDLANPDALRRVVRQCRPDVIVNPAAYTAVDKAETDAEVA
FAINATAAGILAEEAKALGSLLVHYSSDYVFDGTKDGAYVETDAVNPQSVYGKSKLAGEQAIAATGVQSVVLRTCWVAGA
HGGNFAKTMLRLGKERDSLRVIADQFGAPTTASLIADVTAQIVARHWLAGDRQAFANGIYHLAAAGETTWHGYATEVLRY
AKAHGVELKADPEQIEAIPATAYPLPAPRPSNSRLDTTRLQETFGIHLPDWKQGVQFLLDQILP

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=250, Percent_Identity=30.4, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI11034825, Length=250, Percent_Identity=30.4, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788352, Length=299, Percent_Identity=47.8260869565217, Blast_Score=281, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 32616; Mature: 32616

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRKRTVPTLLVTGCNGQVGFELRRSLAPLGNVVALDRSACDLANPDALRRVVRQCRPDV
CCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCE
IVNPAAYTAVDKAETDAEVAFAINATAAGILAEEAKALGSLLVHYSSDYVFDGTKDGAYV
EECCHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEE
ETDAVNPQSVYGKSKLAGEQAIAATGVQSVVLRTCWVAGAHGGNFAKTMLRLGKERDSLR
EECCCCHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHH
VIADQFGAPTTASLIADVTAQIVARHWLAGDRQAFANGIYHLAAAGETTWHGYATEVLRY
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEECCCCCCHHHHHHHHHH
AKAHGVELKADPEQIEAIPATAYPLPAPRPSNSRLDTTRLQETFGIHLPDWKQGVQFLLD
HHHCCCEECCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
QILP
HHCC
>Mature Secondary Structure
MQRKRTVPTLLVTGCNGQVGFELRRSLAPLGNVVALDRSACDLANPDALRRVVRQCRPDV
CCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCE
IVNPAAYTAVDKAETDAEVAFAINATAAGILAEEAKALGSLLVHYSSDYVFDGTKDGAYV
EECCHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEE
ETDAVNPQSVYGKSKLAGEQAIAATGVQSVVLRTCWVAGAHGGNFAKTMLRLGKERDSLR
EECCCCHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHH
VIADQFGAPTTASLIADVTAQIVARHWLAGDRQAFANGIYHLAAAGETTWHGYATEVLRY
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEECCCCCCHHHHHHHHHH
AKAHGVELKADPEQIEAIPATAYPLPAPRPSNSRLDTTRLQETFGIHLPDWKQGVQFLLD
HHHCCCEECCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
QILP
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]