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The map label for this gene is rfbB [H]

Identifier: 73540418

GI number: 73540418

Start: 771726

End: 772790

Strand: Direct

Name: rfbB [H]

Synonym: Reut_A0712

Alternate gene names: 73540418

Gene position: 771726-772790 (Clockwise)

Preceding gene: 73540416

Following gene: 73540419

Centisome position: 20.27

GC content: 59.72

Gene sequence:

>1065_bases
TTGTCGCATATTTTGGTCACAGGCGGTGCCGGTTTCATTGGTGCCAACTTCGTTCTGGCATGGCTGCGGGATAAGGCAGC
CGATGGCATCGTCAACGTCGACAAGCTCACTTACGCGGGCAACCGTAAGACGCTCGCGTCGCTGGATGACGACGGCCGCC
ACGTATTCACGCAGACCGATATCTGCGATCGCGCGGCGCTGGATAACCTTTTCGCGACATACAAGCCGCGCGGGGTGGTG
CATTTCGCGGCCGAAAGCCACGTCGACCGCTCCATCCACGGCCCGAGTGAGTTCATCCAGACCAATATCGTTGGTACCTT
CACGTTACTGGAGGCCGCTCGCGCTTATTGGGGGGCGCTGGAAGAGCCGGCCAAATCTGCGTTCCGCTTTCTTCATGTTT
CCACCGATGAAGTCTTCGGATCGCTCAGCGAAGTCGACCCCCAATTCTCGGAAACCACTCCCTACGCGCCCAACAGCCCG
TATTCGGCATCCAAGGCCGCCTCGGACCATCTGGTCCGTGCCTACCATCACACCTACGGCCTGCCGGTCCTGACGACCAA
CTGTTCGAATAACTACGGCCCCTATCATTTCCCCGAGAAGCTGATCCCGCTGATCATTGCCAACGCGCTGGCCGGAAAGC
CGCTGCCTGTCTACGGTGATGGCCTTAATGTCCGCGATTGGCTGTACGTGGGCGACCACTGCTCCGCAATTCGCGAAGTG
CTTGCTCGCGGCCGGCTCGGTGAAACCTACAACGTAGGCGGCTGGAATGAGAAAACCAATCTCGATGTGGTGCACACGTT
GTGCGATCTGCTTGATGAGCTCAAGCCCAAGGCGGCGGGCTCTTATCGCGACCAGATCACGTTTGTGAAGGATCGCCCGG
GACACGACCGCCGCTACGCGATCGATGCCCGCAAACTGGAGCGCGAACTCGGCTGGAAGCCGGCCGAAACCTTCGAGACG
GGGCTGCGCAAGACGGTGCAGTGGTATCTCGACAACCAGGCGTGGGTGCAGGACGTGATGTCGGGCGAGTATCGGAACTG
GGTGGCGAAACAATATGCAGCGTAA

Upstream 100 bases:

>100_bases
CTTCTGCAGGTACTTAACGCCCCGCCGTCCCCCACGAGGACAAGGCGGGCGCTCGGCTTTTGTCGGGTAATCGGAACATA
TCGAGAGCAGGGAGCTAGTT

Downstream 100 bases:

>100_bases
GCGTACGGTGCCGACTCTACTCGTAACAGGATGCAACGGCCAGGTCGGATTCGAACTGCGTCGCAGCCTGGCCCCGCTGG
GCAATGTGGTCGCGCTGGAT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSHILVTGGAGFIGANFVLAWLRDKAADGIVNVDKLTYAGNRKTLASLDDDGRHVFTQTDICDRAALDNLFATYKPRGVV
HFAAESHVDRSIHGPSEFIQTNIVGTFTLLEAARAYWGALEEPAKSAFRFLHVSTDEVFGSLSEVDPQFSETTPYAPNSP
YSASKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYHFPEKLIPLIIANALAGKPLPVYGDGLNVRDWLYVGDHCSAIREV
LARGRLGETYNVGGWNEKTNLDVVHTLCDLLDELKPKAAGSYRDQITFVKDRPGHDRRYAIDARKLERELGWKPAETFET
GLRKTVQWYLDNQAWVQDVMSGEYRNWVAKQYAA

Sequences:

>Translated_354_residues
MSHILVTGGAGFIGANFVLAWLRDKAADGIVNVDKLTYAGNRKTLASLDDDGRHVFTQTDICDRAALDNLFATYKPRGVV
HFAAESHVDRSIHGPSEFIQTNIVGTFTLLEAARAYWGALEEPAKSAFRFLHVSTDEVFGSLSEVDPQFSETTPYAPNSP
YSASKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYHFPEKLIPLIIANALAGKPLPVYGDGLNVRDWLYVGDHCSAIREV
LARGRLGETYNVGGWNEKTNLDVVHTLCDLLDELKPKAAGSYRDQITFVKDRPGHDRRYAIDARKLERELGWKPAETFET
GLRKTVQWYLDNQAWVQDVMSGEYRNWVAKQYAA
>Mature_353_residues
SHILVTGGAGFIGANFVLAWLRDKAADGIVNVDKLTYAGNRKTLASLDDDGRHVFTQTDICDRAALDNLFATYKPRGVVH
FAAESHVDRSIHGPSEFIQTNIVGTFTLLEAARAYWGALEEPAKSAFRFLHVSTDEVFGSLSEVDPQFSETTPYAPNSPY
SASKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYHFPEKLIPLIIANALAGKPLPVYGDGLNVRDWLYVGDHCSAIREVL
ARGRLGETYNVGGWNEKTNLDVVHTLCDLLDELKPKAAGSYRDQITFVKDRPGHDRRYAIDARKLERELGWKPAETFETG
LRKTVQWYLDNQAWVQDVMSGEYRNWVAKQYAA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=36.1702127659575, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI42516563, Length=334, Percent_Identity=24.5508982035928, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI56237023, Length=359, Percent_Identity=25.6267409470752, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI56118217, Length=359, Percent_Identity=25.6267409470752, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI189083684, Length=359, Percent_Identity=25.6267409470752, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4504031, Length=283, Percent_Identity=25.0883392226148, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788353, Length=357, Percent_Identity=62.7450980392157, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=64.367816091954, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1786974, Length=351, Percent_Identity=27.0655270655271, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788366, Length=179, Percent_Identity=30.7262569832402, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788365, Length=260, Percent_Identity=22.6923076923077, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=334, Percent_Identity=36.5269461077844, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=341, Percent_Identity=33.7243401759531, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI71982038, Length=355, Percent_Identity=28.4507042253521, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71982035, Length=353, Percent_Identity=27.7620396600567, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=334, Percent_Identity=22.7544910179641, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17507723, Length=281, Percent_Identity=25.2669039145907, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=284, Percent_Identity=26.4084507042254, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=284, Percent_Identity=26.4084507042254, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=284, Percent_Identity=26.4084507042254, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=284, Percent_Identity=26.4084507042254, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=284, Percent_Identity=26.4084507042254, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=363, Percent_Identity=25.8953168044077, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19923002, Length=364, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21356223, Length=344, Percent_Identity=24.7093023255814, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24158427, Length=283, Percent_Identity=26.5017667844523, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39482; Mature: 39350

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHILVTGGAGFIGANFVLAWLRDKAADGIVNVDKLTYAGNRKTLASLDDDGRHVFTQTD
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEHHHEEECCCCCEEECCCCCCCEEEEECC
ICDRAALDNLFATYKPRGVVHFAAESHVDRSIHGPSEFIQTNIVGTFTLLEAARAYWGAL
HHHHHHHHHHHHHCCCCCEEEECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EEPAKSAFRFLHVSTDEVFGSLSEVDPQFSETTPYAPNSPYSASKAASDHLVRAYHHTYG
HHHHHHHHHHHCCCHHHHHHCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
LPVLTTNCSNNYGPYHFPEKLIPLIIANALAGKPLPVYGDGLNVRDWLYVGDHCSAIREV
CCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEECHHHHHHHHH
LARGRLGETYNVGGWNEKTNLDVVHTLCDLLDELKPKAAGSYRDQITFVKDRPGHDRRYA
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEE
IDARKLERELGWKPAETFETGLRKTVQWYLDNQAWVQDVMSGEYRNWVAKQYAA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
SHILVTGGAGFIGANFVLAWLRDKAADGIVNVDKLTYAGNRKTLASLDDDGRHVFTQTD
CEEEEECCCCHHHHHHHHHHHHHHHCCCCEEHHHEEECCCCCEEECCCCCCCEEEEECC
ICDRAALDNLFATYKPRGVVHFAAESHVDRSIHGPSEFIQTNIVGTFTLLEAARAYWGAL
HHHHHHHHHHHHHCCCCCEEEECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EEPAKSAFRFLHVSTDEVFGSLSEVDPQFSETTPYAPNSPYSASKAASDHLVRAYHHTYG
HHHHHHHHHHHCCCHHHHHHCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
LPVLTTNCSNNYGPYHFPEKLIPLIIANALAGKPLPVYGDGLNVRDWLYVGDHCSAIREV
CCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEECHHHHHHHHH
LARGRLGETYNVGGWNEKTNLDVVHTLCDLLDELKPKAAGSYRDQITFVKDRPGHDRRYA
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEE
IDARKLERELGWKPAETFETGLRKTVQWYLDNQAWVQDVMSGEYRNWVAKQYAA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503 [H]