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The map label for this gene is kefB [H]

Identifier: 73540066

GI number: 73540066

Start: 389533

End: 391512

Strand: Direct

Name: kefB [H]

Synonym: Reut_A0360

Alternate gene names: 73540066

Gene position: 389533-391512 (Clockwise)

Preceding gene: 73540054

Following gene: 73540069

Centisome position: 10.23

GC content: 65.71

Gene sequence:

>1980_bases
ATGCATTCTCCGCTGGAACTGACCCTGGTGCTGCTGGCTGCCGCCGTATTCGGCGTGGTCGCGTTCCGCATGCTCCAGCT
GCCGCCCATGCTCGGCTACCTGGCGGTCGGCATCCTGATCGGCCCGCATGCGCTGGGCCTGGCCAGCGACACGGCCCAGA
CCAAGTACCTGGCCGAGTTCGGCGTGGTCTTCCTGATGTTTTCGATTGGCCTGGAATTCAGCCTGAGCAAGCTTCGCGCC
ATGAAACGGCTCGTGTTCGGGCTGGGCGGCTCGCAGGTCGTATTGTCGATGCTGGCCGTGGTGCCGGCGAGCTGGCTGTT
CAACTGGCTGTTTCCGTTGTCCTGGCAGGCTTCCGTGGCGCTTGGCGGGGCACTGGCCATGTCGTCGACGGCCATCGTGT
CGAAGATGCTGTCCGAGCGCGTGGAGCTGGAAAGCGAGCATGGCCGCAACATCATCAGCGTGTTGCTGTTCCAGGACCTG
GCCGTGGTGCCGCTGCTGATCGTGATCCCGGCGCTGTCGCGCGACCCCGGTGACCTGATGGTTGCACTCGGCATGGCCAC
GGCGAAGATCGCGGTGGCGCTCGGTGTGATCTTCTTCCTCGGACAGCGCCTGATGAGCCGCTGGTTCCACGTGGTGGCGG
CGCGGCGCTCGCAGGAACTGTTCATGCTGAACCTGCTGCTGGTCACGCTTGGCATGGCTGCGCTGACCGAGCGGCTGGGC
CTGTCGATGGCGCTGGGCGCGTTCATGGCCGGCATGCTGATTTCGGAAACGCCCTACCGGCACCAGGTGGAAGAGGACAT
CAAGCCCTTCCGCGACGTGCTGCTCGGGCTGTTCTTCGTGACCATTGGCATGCTGCTCAACATCCAGGTGGTGATCGAGC
ACATCTGGCTGGTGCTGGTGCTGCTGGGGGTGCCGATCCTGTTCAAGCTGCTGCTGATCTCCGTACTGGCGCGCCTGTTC
GGCTCGCGCCAGGGCGTGGCCATCCGCACGGGCCTGGGGCTCGCGCAGGCCGGCGAATTCGGCTTCGTGCTGCTCAACCA
GATTGACGGCCTCAACCTGGTGGACCCGGTGCTGATCCAGGTGATCCTGGCGTCGATGCTGCTCTCGATGCTGGCCGCGC
CGTTCCTGATCCAGTACAGCGATGCCATTGTGCTGCGCTTTGCGGCGAACGAATGGCTGATGCAGTCGCTGAACATGACG
CGTATCGCCGCGCAGAGCCTGCAGACCGAGAAGCACGCGATCATCTGCGGTTACGGGCGCAGCGGCCAGAACCTGGCGCA
TATGCTCGAGCGCGAGAGCATCACTTACGTGGCGCTCGACCTTGATCCGGACCGCGTGCGCGATGCCGCCGCGGCGGGCG
ATACCGTGGTCTACGGTGACGCTGGGCGTCGCGAGGCGCTGATTGCCGCCGGTATCCACCGCGCCGCCGCCGTCATCATC
ACCTATGCCAACACGCCGTCAGCGCTCAAGGTGCTGCACCATGTGCAGGAGTTGGCGCCGGCGTTGCCGGTGATCGTACG
CACGGTCGACGATTCTGAACTCGACACGTTGCAGAAAGCGGGTGCCACCGAAGTCGTGCCGGAAATCATCGAGGGCAGCC
TGATGCTGGCTTCGCATGCGCTGGTGCTGCTCGGGGTGCCCATGCGTCACGTGTTGCGTGGCGTGCAGGAAGCACGCGAT
GCGCGCTACAGCCTGCTGCGCGGCTACTTTCACGGCCGCGACGATGAAGAGGACATGGTCGAGCGCGATAGCGTGCGGCT
GCATTCGGTGTCGCTCGAGCCGGCCGCGACGGCCGTCGGCCGCCGCTTGGGCGCGTTGGGGTTGGAGCGAATCGGCGCCG
AAGTCACGGCCGTTCGCCGCCGTGGCATCCGCGCCTTCGATCCGGAGCCCGACACCATCCTCGAAGTCGGCGACATCATC
GTGCTGCGTGGCACGCCGGAGGCACTGGAGGCGGCGGAGACGCGGATCCTGAGCGACTGA

Upstream 100 bases:

>100_bases
CCGGGCCGTCCCGGCAAGCGCCGCGCTTGCCGTGTTCCGCCGGCAGGGCCCGTGCCGGGGCGCAGCCGGCCAGCCCTGAC
GGTTCTTCCTGAGCCGCCTG

Downstream 100 bases:

>100_bases
TCTGGCTGGAGTCCCCTAGTGCGCCGCTCCGGCTGGCGCTTCCGCCCACGACGCCGGCAGGTTGTCCAGCACAGACCGGG
CGTCGAGAGAGCGGATCGCG

Product: TrkA-N:TrkA-C:Sodium/hydrogen exchanger

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MHSPLELTLVLLAAAVFGVVAFRMLQLPPMLGYLAVGILIGPHALGLASDTAQTKYLAEFGVVFLMFSIGLEFSLSKLRA
MKRLVFGLGGSQVVLSMLAVVPASWLFNWLFPLSWQASVALGGALAMSSTAIVSKMLSERVELESEHGRNIISVLLFQDL
AVVPLLIVIPALSRDPGDLMVALGMATAKIAVALGVIFFLGQRLMSRWFHVVAARRSQELFMLNLLLVTLGMAALTERLG
LSMALGAFMAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTIGMLLNIQVVIEHIWLVLVLLGVPILFKLLLISVLARLF
GSRQGVAIRTGLGLAQAGEFGFVLLNQIDGLNLVDPVLIQVILASMLLSMLAAPFLIQYSDAIVLRFAANEWLMQSLNMT
RIAAQSLQTEKHAIICGYGRSGQNLAHMLERESITYVALDLDPDRVRDAAAAGDTVVYGDAGRREALIAAGIHRAAAVII
TYANTPSALKVLHHVQELAPALPVIVRTVDDSELDTLQKAGATEVVPEIIEGSLMLASHALVLLGVPMRHVLRGVQEARD
ARYSLLRGYFHGRDDEEDMVERDSVRLHSVSLEPAATAVGRRLGALGLERIGAEVTAVRRRGIRAFDPEPDTILEVGDII
VLRGTPEALEAAETRILSD

Sequences:

>Translated_659_residues
MHSPLELTLVLLAAAVFGVVAFRMLQLPPMLGYLAVGILIGPHALGLASDTAQTKYLAEFGVVFLMFSIGLEFSLSKLRA
MKRLVFGLGGSQVVLSMLAVVPASWLFNWLFPLSWQASVALGGALAMSSTAIVSKMLSERVELESEHGRNIISVLLFQDL
AVVPLLIVIPALSRDPGDLMVALGMATAKIAVALGVIFFLGQRLMSRWFHVVAARRSQELFMLNLLLVTLGMAALTERLG
LSMALGAFMAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTIGMLLNIQVVIEHIWLVLVLLGVPILFKLLLISVLARLF
GSRQGVAIRTGLGLAQAGEFGFVLLNQIDGLNLVDPVLIQVILASMLLSMLAAPFLIQYSDAIVLRFAANEWLMQSLNMT
RIAAQSLQTEKHAIICGYGRSGQNLAHMLERESITYVALDLDPDRVRDAAAAGDTVVYGDAGRREALIAAGIHRAAAVII
TYANTPSALKVLHHVQELAPALPVIVRTVDDSELDTLQKAGATEVVPEIIEGSLMLASHALVLLGVPMRHVLRGVQEARD
ARYSLLRGYFHGRDDEEDMVERDSVRLHSVSLEPAATAVGRRLGALGLERIGAEVTAVRRRGIRAFDPEPDTILEVGDII
VLRGTPEALEAAETRILSD
>Mature_659_residues
MHSPLELTLVLLAAAVFGVVAFRMLQLPPMLGYLAVGILIGPHALGLASDTAQTKYLAEFGVVFLMFSIGLEFSLSKLRA
MKRLVFGLGGSQVVLSMLAVVPASWLFNWLFPLSWQASVALGGALAMSSTAIVSKMLSERVELESEHGRNIISVLLFQDL
AVVPLLIVIPALSRDPGDLMVALGMATAKIAVALGVIFFLGQRLMSRWFHVVAARRSQELFMLNLLLVTLGMAALTERLG
LSMALGAFMAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTIGMLLNIQVVIEHIWLVLVLLGVPILFKLLLISVLARLF
GSRQGVAIRTGLGLAQAGEFGFVLLNQIDGLNLVDPVLIQVILASMLLSMLAAPFLIQYSDAIVLRFAANEWLMQSLNMT
RIAAQSLQTEKHAIICGYGRSGQNLAHMLERESITYVALDLDPDRVRDAAAAGDTVVYGDAGRREALIAAGIHRAAAVII
TYANTPSALKVLHHVQELAPALPVIVRTVDDSELDTLQKAGATEVVPEIIEGSLMLASHALVLLGVPMRHVLRGVQEARD
ARYSLLRGYFHGRDDEEDMVERDSVRLHSVSLEPAATAVGRRLGALGLERIGAEVTAVRRRGIRAFDPEPDTILEVGDII
VLRGTPEALEAAETRILSD

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=340, Percent_Identity=30, Blast_Score=109, Evalue=1e-23,
Organism=Escherichia coli, GI1789749, Length=564, Percent_Identity=34.7517730496454, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1786232, Length=590, Percent_Identity=32.7118644067797, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1786685, Length=538, Percent_Identity=31.0408921933086, Blast_Score=207, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 71475; Mature: 71475

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSPLELTLVLLAAAVFGVVAFRMLQLPPMLGYLAVGILIGPHALGLASDTAQTKYLAEF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHH
GVVFLMFSIGLEFSLSKLRAMKRLVFGLGGSQVVLSMLAVVPASWLFNWLFPLSWQASVA
HHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LGGALAMSSTAIVSKMLSERVELESEHGRNIISVLLFQDLAVVPLLIVIPALSRDPGDLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VALGMATAKIAVALGVIFFLGQRLMSRWFHVVAARRSQELFMLNLLLVTLGMAALTERLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LSMALGAFMAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTIGMLLNIQVVIEHIWLVLV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGVPILFKLLLISVLARLFGSRQGVAIRTGLGLAQAGEFGFVLLNQIDGLNLVDPVLIQ
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCCCCCCCHHHHCCCCCCHHHHHHHH
VILASMLLSMLAAPFLIQYSDAIVLRFAANEWLMQSLNMTRIAAQSLQTEKHAIICGYGR
HHHHHHHHHHHHHHHHHEECCEEEEEEHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCC
SGQNLAHMLERESITYVALDLDPDRVRDAAAAGDTVVYGDAGRREALIAAGIHRAAAVII
CCHHHHHHHHHCCEEEEEEECCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHEEEEE
TYANTPSALKVLHHVQELAPALPVIVRTVDDSELDTLQKAGATEVVPEIIEGSLMLASHA
EECCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCHHHHHHHHCCCHHHHHHH
LVLLGVPMRHVLRGVQEARDARYSLLRGYFHGRDDEEDMVERDSVRLHSVSLEPAATAVG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEECCCHHHHHHH
RRLGALGLERIGAEVTAVRRRGIRAFDPEPDTILEVGDIIVLRGTPEALEAAETRILSD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEECCEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MHSPLELTLVLLAAAVFGVVAFRMLQLPPMLGYLAVGILIGPHALGLASDTAQTKYLAEF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHH
GVVFLMFSIGLEFSLSKLRAMKRLVFGLGGSQVVLSMLAVVPASWLFNWLFPLSWQASVA
HHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LGGALAMSSTAIVSKMLSERVELESEHGRNIISVLLFQDLAVVPLLIVIPALSRDPGDLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VALGMATAKIAVALGVIFFLGQRLMSRWFHVVAARRSQELFMLNLLLVTLGMAALTERLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LSMALGAFMAGMLISETPYRHQVEEDIKPFRDVLLGLFFVTIGMLLNIQVVIEHIWLVLV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGVPILFKLLLISVLARLFGSRQGVAIRTGLGLAQAGEFGFVLLNQIDGLNLVDPVLIQ
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCCCCCCCHHHHCCCCCCHHHHHHHH
VILASMLLSMLAAPFLIQYSDAIVLRFAANEWLMQSLNMTRIAAQSLQTEKHAIICGYGR
HHHHHHHHHHHHHHHHHEECCEEEEEEHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCC
SGQNLAHMLERESITYVALDLDPDRVRDAAAAGDTVVYGDAGRREALIAAGIHRAAAVII
CCHHHHHHHHHCCEEEEEEECCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHEEEEE
TYANTPSALKVLHHVQELAPALPVIVRTVDDSELDTLQKAGATEVVPEIIEGSLMLASHA
EECCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCHHHHHHHHCCCHHHHHHH
LVLLGVPMRHVLRGVQEARDARYSLLRGYFHGRDDEEDMVERDSVRLHSVSLEPAATAVG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEECCCHHHHHHH
RRLGALGLERIGAEVTAVRRRGIRAFDPEPDTILEVGDIIVLRGTPEALEAAETRILSD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEECCEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA