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The map label for this gene is mutY [H]

Identifier: 73540054

GI number: 73540054

Start: 378816

End: 379997

Strand: Direct

Name: mutY [H]

Synonym: Reut_A0348

Alternate gene names: 73540054

Gene position: 378816-379997 (Clockwise)

Preceding gene: 73540053

Following gene: 73540066

Centisome position: 9.95

GC content: 69.88

Gene sequence:

>1182_bases
ATGCCCCGCAAGTCCGCCCCCGCCGTCCCGCCCACTGCCAATGCCTCTGCCGTGCCGTCCGTCATCCACGTTCCTGCCGA
TTTCGGCGCACGCGTGGTCGAATGGCAGCGCGTGCACGGCCGGCATGACCTGCCGTGGCAGAACACGCGCGACGCGTACC
GGATCTGGCTGTCGGAGATCATGCTGCAGCAGACCCAGGTCAGCGCGGTGATCGATTACTTCCAGCGCTTCATCACGCAA
CTGCCCACGGTGCAGGCGCTGGCCGCCGCGCCCGCCGACCAGGTGATGGCGCTGTGGGCGGGGCTGGGCTACTACTCGCG
CGCGCGCAACCTGCACCGCTGCGCGATGCAGGTCGTCAGCGAGCATGGGGGTCGTTTTCCGCCCGATCCCGCAGTACTGG
CCACGCTGCCGGGCATTGGCCGATCGACGGCCGCGGCCGTCGCGGCGTTCAGTGCGGGCGTGCGCTCGCCGATTCTCGAC
GGCAACGTCAAGCGCGTATTCGCGCGCTTCTTCGGCATCCATGGGCACCCCGGCGAACGTGCGATCGAGAACCGCATGTG
GGAGCTGGCTGACGCCGCGTTGCCCGCCCCGGGCCCGCACCAGGCCGACGACATGGTGGCCTACACGCAGGGCCTGATGG
ACCTTGGGGCTACCGTGTGTTCGCGCGGCAAGCCCGCCTGCCTGTCCGATGCCGCGGCCTGCCCGTTGTCGTCGGACTGC
GTGGCGCGGCGCGATGGCCTGACCGCGACGCTGCCCACGCCCAAGCCGCGCTCGCCGGTGCCCGAGCGCAGCACCGTGAT
GGTCATGGTGCGTCACGGACGCGACGTGCTGCTGCAGCTTCGGCCTGAAAGCGGCGTCTGGGGCGGCCTGTGGAGCCTGC
CCGAGATGCCTGTCGCCACGGTGCCGTTCGACGCGGAACTCGCCGAGCAGGATGCGCTGGGTTACGCGCGTGCGTTTGGG
GAACCGTCGCGCGCGGACATGACCGGCGAACTGGTCCACGTCTTCACGCATTTCCGTCTACTGATCCGCGCCATCCGCGT
CGACATGAAGGGTCTGTTGCTGCGCGAGCCGGTCGACGGACCGGCGCAGCGCTGGATCTCGCTCGATGATCTCGATGCGC
TCGGCACGCCGGCGCCCGTGCGCAAGTTGCTCGAAACGCAGGCCCGCGGCGGCCTGTTCTGA

Upstream 100 bases:

>100_bases
CCGGTATATCATGTGGCGCCGCCTCTGTGCGGCGCCATTTCTTTTGTGCGGCCCGCGCCTTGTTTGCCTGCCGCGCAGTC
CAAGCGCCTGAACGTCCCCG

Downstream 100 bases:

>100_bases
ACCTCGAAAGGGGCGCAGCGAGCCCTACACCATGTGGTGCTGCCGCATGTAGCGGTGCACGATCTCGATGCGCATGCCCG
CGTCGGGCAACGCCATCAGC

Product: A/G-specific DNA-adenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MPRKSAPAVPPTANASAVPSVIHVPADFGARVVEWQRVHGRHDLPWQNTRDAYRIWLSEIMLQQTQVSAVIDYFQRFITQ
LPTVQALAAAPADQVMALWAGLGYYSRARNLHRCAMQVVSEHGGRFPPDPAVLATLPGIGRSTAAAVAAFSAGVRSPILD
GNVKRVFARFFGIHGHPGERAIENRMWELADAALPAPGPHQADDMVAYTQGLMDLGATVCSRGKPACLSDAAACPLSSDC
VARRDGLTATLPTPKPRSPVPERSTVMVMVRHGRDVLLQLRPESGVWGGLWSLPEMPVATVPFDAELAEQDALGYARAFG
EPSRADMTGELVHVFTHFRLLIRAIRVDMKGLLLREPVDGPAQRWISLDDLDALGTPAPVRKLLETQARGGLF

Sequences:

>Translated_393_residues
MPRKSAPAVPPTANASAVPSVIHVPADFGARVVEWQRVHGRHDLPWQNTRDAYRIWLSEIMLQQTQVSAVIDYFQRFITQ
LPTVQALAAAPADQVMALWAGLGYYSRARNLHRCAMQVVSEHGGRFPPDPAVLATLPGIGRSTAAAVAAFSAGVRSPILD
GNVKRVFARFFGIHGHPGERAIENRMWELADAALPAPGPHQADDMVAYTQGLMDLGATVCSRGKPACLSDAAACPLSSDC
VARRDGLTATLPTPKPRSPVPERSTVMVMVRHGRDVLLQLRPESGVWGGLWSLPEMPVATVPFDAELAEQDALGYARAFG
EPSRADMTGELVHVFTHFRLLIRAIRVDMKGLLLREPVDGPAQRWISLDDLDALGTPAPVRKLLETQARGGLF
>Mature_392_residues
PRKSAPAVPPTANASAVPSVIHVPADFGARVVEWQRVHGRHDLPWQNTRDAYRIWLSEIMLQQTQVSAVIDYFQRFITQL
PTVQALAAAPADQVMALWAGLGYYSRARNLHRCAMQVVSEHGGRFPPDPAVLATLPGIGRSTAAAVAAFSAGVRSPILDG
NVKRVFARFFGIHGHPGERAIENRMWELADAALPAPGPHQADDMVAYTQGLMDLGATVCSRGKPACLSDAAACPLSSDCV
ARRDGLTATLPTPKPRSPVPERSTVMVMVRHGRDVLLQLRPESGVWGGLWSLPEMPVATVPFDAELAEQDALGYARAFGE
PSRADMTGELVHVFTHFRLLIRAIRVDMKGLLLREPVDGPAQRWISLDDLDALGTPAPVRKLLETQARGGLF

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298648, Length=416, Percent_Identity=31.0096153846154, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI115298654, Length=416, Percent_Identity=31.0096153846154, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI115298652, Length=416, Percent_Identity=31.0096153846154, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI190358497, Length=416, Percent_Identity=31.0096153846154, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI6912520, Length=416, Percent_Identity=31.0096153846154, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI115298650, Length=416, Percent_Identity=31.0096153846154, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1789331, Length=368, Percent_Identity=40.7608695652174, Blast_Score=286, Evalue=1e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 42654; Mature: 42522

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRKSAPAVPPTANASAVPSVIHVPADFGARVVEWQRVHGRHDLPWQNTRDAYRIWLSEI
CCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
MLQQTQVSAVIDYFQRFITQLPTVQALAAAPADQVMALWAGLGYYSRARNLHRCAMQVVS
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EHGGRFPPDPAVLATLPGIGRSTAAAVAAFSAGVRSPILDGNVKRVFARFFGIHGHPGER
HHCCCCCCCCHHEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHH
AIENRMWELADAALPAPGPHQADDMVAYTQGLMDLGATVCSRGKPACLSDAAACPLSSDC
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHCCCCCCHH
VARRDGLTATLPTPKPRSPVPERSTVMVMVRHGRDVLLQLRPESGVWGGLWSLPEMPVAT
HHHHCCCEEECCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCEEE
VPFDAELAEQDALGYARAFGEPSRADMTGELVHVFTHFRLLIRAIRVDMKGLLLREPVDG
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PAQRWISLDDLDALGTPAPVRKLLETQARGGLF
HHHHCCCHHHHHHCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PRKSAPAVPPTANASAVPSVIHVPADFGARVVEWQRVHGRHDLPWQNTRDAYRIWLSEI
CCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
MLQQTQVSAVIDYFQRFITQLPTVQALAAAPADQVMALWAGLGYYSRARNLHRCAMQVVS
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EHGGRFPPDPAVLATLPGIGRSTAAAVAAFSAGVRSPILDGNVKRVFARFFGIHGHPGER
HHCCCCCCCCHHEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHH
AIENRMWELADAALPAPGPHQADDMVAYTQGLMDLGATVCSRGKPACLSDAAACPLSSDC
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHCCCCCCHH
VARRDGLTATLPTPKPRSPVPERSTVMVMVRHGRDVLLQLRPESGVWGGLWSLPEMPVAT
HHHHCCCEEECCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCEEE
VPFDAELAEQDALGYARAFGEPSRADMTGELVHVFTHFRLLIRAIRVDMKGLLLREPVDG
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PAQRWISLDDLDALGTPAPVRKLLETQARGGLF
HHHHCCCHHHHHHCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]