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The map label for this gene is dctA [H]

Identifier: 73539983

GI number: 73539983

Start: 309548

End: 310843

Strand: Reverse

Name: dctA [H]

Synonym: Reut_A0277

Alternate gene names: 73539983

Gene position: 310843-309548 (Counterclockwise)

Preceding gene: 73539989

Following gene: 73539982

Centisome position: 8.17

GC content: 62.96

Gene sequence:

>1296_bases
ATGAGGAAACCCTTCTACAAGATCCTGTACGTGCAGGTGCTGTTCGCCATCTTCGTCGGCATCCTGCTGGGCCATTTCTG
GCCCGACACAGGCGTTGCCATGAAGCCGCTCGGCGATGGCTTCATCAAGCTGATCAAGATGATCATCGGGCCCATCATCT
TCTGTACCGTGGTGACCGGCATCGCCGGCATGAGCGACATGAAGAAGGTCGGCCGGGTCGGCGGCAAGGCGCTGCTGTAC
TTCGAGATCGTGTCGACATTCGCGCTGCTGATCGGCCTGGGCGCGGCGCACCTGCTCAAGCCCGGCGTGGGCTTCAACAT
CGACCCGGCCACGCTGGACACCAAGGCGATCGAGCAGTACGTGTCCAAGGCTCACGGCCAGAGCACGGTCGAGTTCCTGA
TGCACATCATCCCGGACACGATCTTCAGCGCGTTCTCCAACGGCGACATCCTGCAGATCCTGCTGGTTTCGCTGTTCTTC
GGCGCCGCGCTGGCCGCCATCGGCGAACGTGCCCGCATCGTCGTCCAGCTGATCGAGCAGGTGTCGAAGGTGTTCTTCCA
CATCGTGCACGTCATCACCAAGGTCGCGCCGATCGGCGCGTTCGGCGCGATGGCCTTTACCATCGGCAAGTACGGCCTGG
GCTCGCTGGTGCCGCTGCTCAAGCTGATCGGCACGTTCTACTTCACGGCCATCGTATTCGTGGTGTTCGTGCTGGGTGCG
ATCGCGCGCGCGACCGGTTTCAACATCCTGCGCTTCATCTCGTACATCAAGGAAGAGCTGCTGATCGTGCTGGGCACGAG
CTCGTCCGAAGCCGCGCTGCCGCACCTGATGGAAAAGCTGGAAAAGCTCGGCTGCTCCAAGTCGGTGGTCGGCCTGGTCG
TGCCGACCGGCTACTCGTTCAACCTGGACGGTACCAACATCTACATGACCATGGCGGTGATCTTCATCGCGCAGGCCACC
GGCATCGAACTGACGCTGTTGCAGCAACTCACCATCCTCGGGGTGGCCATGCTCACGTCCAAGGGTGCCAGCGGCGTGAC
CGGCGCGGGCTTTATCACGCTGGCCGCGACGCTGGCCGTGGTGCCGACCATTCCGGTGGCCGGCATGGTGCTGATCCTCG
GCATCGACCGGTTCATGAGCGAATGCCGTGCGCTGACCAACATCATCGGCAACGGCGTGGCCACCGTGGTGGTCTCGGCC
TGGGAGCACGAGCTTGACCGCGCCCAGCTCAAGCGCACGCTGCAGCAAGGTGGCGAGGATGAGGCAGAACTCGCCGACGC
CGGCCAGAACGTCTGA

Upstream 100 bases:

>100_bases
ATAGCCATTGCGATTCCAGACCCCGAGACGCGCCGCATCCGTGACGGCGCGCGCCCGCCGCCCCGGCGGGTCACCGCCCA
AAAGAACCGAGGACGACATC

Downstream 100 bases:

>100_bases
CGCCTCGCAGGCAAGCGGCCGGCCCGGTGGGCCGGCCGCGCCGCAGCGAGTATCATCGGCGCGACAGCCTTCGCGCGCGC
CGATGCCCCATTCCGACGAC

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MRKPFYKILYVQVLFAIFVGILLGHFWPDTGVAMKPLGDGFIKLIKMIIGPIIFCTVVTGIAGMSDMKKVGRVGGKALLY
FEIVSTFALLIGLGAAHLLKPGVGFNIDPATLDTKAIEQYVSKAHGQSTVEFLMHIIPDTIFSAFSNGDILQILLVSLFF
GAALAAIGERARIVVQLIEQVSKVFFHIVHVITKVAPIGAFGAMAFTIGKYGLGSLVPLLKLIGTFYFTAIVFVVFVLGA
IARATGFNILRFISYIKEELLIVLGTSSSEAALPHLMEKLEKLGCSKSVVGLVVPTGYSFNLDGTNIYMTMAVIFIAQAT
GIELTLLQQLTILGVAMLTSKGASGVTGAGFITLAATLAVVPTIPVAGMVLILGIDRFMSECRALTNIIGNGVATVVVSA
WEHELDRAQLKRTLQQGGEDEAELADAGQNV

Sequences:

>Translated_431_residues
MRKPFYKILYVQVLFAIFVGILLGHFWPDTGVAMKPLGDGFIKLIKMIIGPIIFCTVVTGIAGMSDMKKVGRVGGKALLY
FEIVSTFALLIGLGAAHLLKPGVGFNIDPATLDTKAIEQYVSKAHGQSTVEFLMHIIPDTIFSAFSNGDILQILLVSLFF
GAALAAIGERARIVVQLIEQVSKVFFHIVHVITKVAPIGAFGAMAFTIGKYGLGSLVPLLKLIGTFYFTAIVFVVFVLGA
IARATGFNILRFISYIKEELLIVLGTSSSEAALPHLMEKLEKLGCSKSVVGLVVPTGYSFNLDGTNIYMTMAVIFIAQAT
GIELTLLQQLTILGVAMLTSKGASGVTGAGFITLAATLAVVPTIPVAGMVLILGIDRFMSECRALTNIIGNGVATVVVSA
WEHELDRAQLKRTLQQGGEDEAELADAGQNV
>Mature_431_residues
MRKPFYKILYVQVLFAIFVGILLGHFWPDTGVAMKPLGDGFIKLIKMIIGPIIFCTVVTGIAGMSDMKKVGRVGGKALLY
FEIVSTFALLIGLGAAHLLKPGVGFNIDPATLDTKAIEQYVSKAHGQSTVEFLMHIIPDTIFSAFSNGDILQILLVSLFF
GAALAAIGERARIVVQLIEQVSKVFFHIVHVITKVAPIGAFGAMAFTIGKYGLGSLVPLLKLIGTFYFTAIVFVVFVLGA
IARATGFNILRFISYIKEELLIVLGTSSSEAALPHLMEKLEKLGCSKSVVGLVVPTGYSFNLDGTNIYMTMAVIFIAQAT
GIELTLLQQLTILGVAMLTSKGASGVTGAGFITLAATLAVVPTIPVAGMVLILGIDRFMSECRALTNIIGNGVATVVVSA
WEHELDRAQLKRTLQQGGEDEAELADAGQNV

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI21314632, Length=429, Percent_Identity=24.7086247086247, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI169790839, Length=456, Percent_Identity=25.4385964912281, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI4827012, Length=281, Percent_Identity=30.2491103202847, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI40254478, Length=452, Percent_Identity=25, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI5032093, Length=404, Percent_Identity=25.4950495049505, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI66773030, Length=411, Percent_Identity=24.0875912408759, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI194239697, Length=462, Percent_Identity=24.025974025974, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI223468564, Length=290, Percent_Identity=26.551724137931, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI223468566, Length=290, Percent_Identity=26.551724137931, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI301601644, Length=197, Percent_Identity=27.9187817258883, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI262359914, Length=376, Percent_Identity=23.6702127659574, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1789947, Length=420, Percent_Identity=61.6666666666667, Blast_Score=530, Evalue=1e-151,
Organism=Escherichia coli, GI1790514, Length=412, Percent_Identity=38.5922330097087, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI1788024, Length=413, Percent_Identity=23.2445520581114, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17537407, Length=433, Percent_Identity=26.3279445727483, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71983106, Length=425, Percent_Identity=24.9411764705882, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71983099, Length=425, Percent_Identity=24.9411764705882, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI193206505, Length=473, Percent_Identity=24.5243128964059, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI193206654, Length=458, Percent_Identity=22.9257641921397, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71996953, Length=444, Percent_Identity=24.3243243243243, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17541374, Length=439, Percent_Identity=22.7790432801822, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24583025, Length=439, Percent_Identity=22.5512528473804, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI17137668, Length=439, Percent_Identity=22.5512528473804, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24583023, Length=439, Percent_Identity=22.5512528473804, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI281360483, Length=470, Percent_Identity=23.8297872340426, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17137666, Length=470, Percent_Identity=23.8297872340426, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281360481, Length=470, Percent_Identity=23.8297872340426, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45986; Mature: 45986

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPFYKILYVQVLFAIFVGILLGHFWPDTGVAMKPLGDGFIKLIKMIIGPIIFCTVVTG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IAGMSDMKKVGRVGGKALLYFEIVSTFALLIGLGAAHLLKPGVGFNIDPATLDTKAIEQY
HCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
VSKAHGQSTVEFLMHIIPDTIFSAFSNGDILQILLVSLFFGAALAAIGERARIVVQLIEQ
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSKVFFHIVHVITKVAPIGAFGAMAFTIGKYGLGSLVPLLKLIGTFYFTAIVFVVFVLGA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARATGFNILRFISYIKEELLIVLGTSSSEAALPHLMEKLEKLGCSKSVVGLVVPTGYSF
HHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCEEE
NLDGTNIYMTMAVIFIAQATGIELTLLQQLTILGVAMLTSKGASGVTGAGFITLAATLAV
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VPTIPVAGMVLILGIDRFMSECRALTNIIGNGVATVVVSAWEHELDRAQLKRTLQQGGED
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EAELADAGQNV
HHHHHHCCCCC
>Mature Secondary Structure
MRKPFYKILYVQVLFAIFVGILLGHFWPDTGVAMKPLGDGFIKLIKMIIGPIIFCTVVTG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IAGMSDMKKVGRVGGKALLYFEIVSTFALLIGLGAAHLLKPGVGFNIDPATLDTKAIEQY
HCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
VSKAHGQSTVEFLMHIIPDTIFSAFSNGDILQILLVSLFFGAALAAIGERARIVVQLIEQ
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSKVFFHIVHVITKVAPIGAFGAMAFTIGKYGLGSLVPLLKLIGTFYFTAIVFVVFVLGA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARATGFNILRFISYIKEELLIVLGTSSSEAALPHLMEKLEKLGCSKSVVGLVVPTGYSF
HHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCEEE
NLDGTNIYMTMAVIFIAQATGIELTLLQQLTILGVAMLTSKGASGVTGAGFITLAATLAV
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VPTIPVAGMVLILGIDRFMSECRALTNIIGNGVATVVVSAWEHELDRAQLKRTLQQGGED
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EAELADAGQNV
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA