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The map label for this gene is pcrA [H]

Identifier: 73539989

GI number: 73539989

Start: 315429

End: 317606

Strand: Reverse

Name: pcrA [H]

Synonym: Reut_A0283

Alternate gene names: 73539989

Gene position: 317606-315429 (Counterclockwise)

Preceding gene: 73539990

Following gene: 73539983

Centisome position: 8.34

GC content: 67.03

Gene sequence:

>2178_bases
ATGGATATACAGTTTGCGCCAGTCTCATCCGCCCTGCGCCCCGGCGCTTCCGAACCGCCCGTGTCACTGGATCTTGCCCC
TCTCGCGGAACGCCCGTCCGCTCCCGCCGCAGTCGTCTCCCCGGACGCTGATGTTCCGGCATACCTGTCGCGGCTCAATC
CGGAGCAACGCGCGGCGGTCGAGCACCACAGCGACGGACCGCTGCTGATCATCGCCGGCGCAGGCTCGGGCAAGACCAAC
ACGCTCGCGCACCGCGTCGCGCATCTGGTGCTCAATGGCGCCGATCCGCGCCGCATCCTGCTGCTCACGTTCTCGCGACG
CGCCGCGGCGGAGATGGGGCGGCGTGTGGAGCGCATCGTCGATCAGGCGCTTGGCACCAATACGGGCGCAGGCCGCGCCG
CGCTGCAGTGGTCGGGCACATTCCACGCGATCGGCGCGCGCCTGTTGCGCGAGTATGCAGAAACGCTGGGCCTGTCGCCC
GCCTTCACGATCTGCGACCGCGGCGACGCCGCCGACCTGATGCACGTCGTGCGGCACGATCTCGGACTGTCCGAGCAGGC
CAGCCGCTTCCCGAAAAAGGAAACCTGCCTGTCGATCTATTCGCGCGTGGTCAACACGCAGGCGCCGCTCGAAGACGTGC
TCAAGCACTGGTTCCCGCGCTACACCATGTGGGTAGATGCGCTGCGCACGCTGTTCGCGGGCTATGTGGAGGCCAAGCAG
AAGCAGCAGGTGCTCGACTACGACGACCTGCTGCTGTACTGGGCGCAGGCGCTGACGGAACCCGCGATCGCGCAGGACAT
GGGTGCGCGCTTCGACCATATCCTTGTCGATGAATACCAGGACACCAATGCGCTGCAGGCTTCTATCCTGCTGGCGTTGC
GGCCCGACGGGCGCGGCCTGACCGTGGTCGGCGATGACGCGCAGTCGATCTACGCGTTCCGCGGCGCAACCGTGCGCAAT
ATCCTCGATTTCCCGGCACAGTTCACGCCGGCCGCGCATCAGGTCACGCTGTCGCAGAATTACCGTTCGACGCAGCCGAT
CCTGCACGCCGCCAATGCGGTGATCGGGCTGGCTGCCGAGCGCTACACGAAGGATCTGTGGTCGGAGCGGACCTCGGCCG
AGAAGCCCGGCGTGGTCGTCGTCAACGATGAAGCCGACCAGGCCCGCTTCGTGGTCGAGCGCGTGCTCGAGCGCCGCGAG
GCAGGCCTCTCGCTGATGTCGCAAGCGGTGCTGTTCCGCGCCGCCGACCATAGCGCGCAGGTCGAGATCGAGTTGGCCCG
GCGCAATATCCCGTTCGTGAAGTTCGGCGGGCTCAAGTTCCTCGAATCCACGCACGTGAAGGATGCGATGGCGGTGGTAC
GCTGGCTCGAAAATCCGCTCGATCGCATCGCTGGCTTCCGCACGCTGCAGTTGCTGCCCGGCATCGGGCCCAAGACCGCG
GCGCGCGTACTCGATGCGCTCGCGGTCGCCACGGAGCCGCTCTTCGCGCTAGAATCGTTCGAGCCGCCGCCTGCTGCCGC
GCCGGCCTGGACAGACCTGCTGACACTGGCCCGCACGCTGCTGGGGCCCGCATCACCGTGGCCGTCGGAGTTCGAGCAGG
TTGTCGCCTGGTATCAACCGCACCTGGAACGCATCCACGACGATGCCGCCGCGCGCATGGCCGACCTGCAGCAGCTCGAA
CGCATTGCCGCGACCTATGCCTCGCGCGAGCGCTTCCTGACCGAGCTGACGCTCGACCCGCCGAGCGCGTCCAGCGATGA
ATCGGGCGTACCGCTGCGCGACGAGGATTTCCTGATCCTGTCGACCATCCACTCGGCCAAGGGCCAGGAATGGAAGGCGG
TGTACGTGCTCAATGCCGTGGATGGCTGCATGCCGTCGGACCTGGCCACCGGCACCACGGAAGAGATCGAAGAGGAAAGA
CGGCTGCTGTATGTGGCGATGACGCGCGCCAAGGACCATCTGGATGTCGTCGTGCCGCAGCGCTTCTACGTGCATCAGCA
GCCGGGGTTCGGCGACCGGCACGTCTATGCGTCGCGCACGCGCTTCCTGCCGAATCGCGTGATGCCGAACTTCTACAGCC
GGTCCTGGCCGCCTCCGCCGATGCCGGGGGAGGCGCCGTCGAAGGCTACGCTGGCGCCGGTGGACCTGGCCAGCAGGATG
CGGAATATGTGGCGGTGA

Upstream 100 bases:

>100_bases
CCGGCAACGGCAGCGAAAGTTACGAAAAAGGTGCTCATCGAGCACCTTTTTTGTTGGGCGGCTACCGCGTTCGCTTAAGC
GGCTTGCGCCACCGGCCTGT

Downstream 100 bases:

>100_bases
GCCTCACGCTTCCGCCGTATCCCTTTACGCCACCTTCTCCAGCAGCAACCCCTTCAGGTACTCCCCTTCCGGGAACGCCG
CCAGCATCGGGTGATCCGTG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 725; Mature: 725

Protein sequence:

>725_residues
MDIQFAPVSSALRPGASEPPVSLDLAPLAERPSAPAAVVSPDADVPAYLSRLNPEQRAAVEHHSDGPLLIIAGAGSGKTN
TLAHRVAHLVLNGADPRRILLLTFSRRAAAEMGRRVERIVDQALGTNTGAGRAALQWSGTFHAIGARLLREYAETLGLSP
AFTICDRGDAADLMHVVRHDLGLSEQASRFPKKETCLSIYSRVVNTQAPLEDVLKHWFPRYTMWVDALRTLFAGYVEAKQ
KQQVLDYDDLLLYWAQALTEPAIAQDMGARFDHILVDEYQDTNALQASILLALRPDGRGLTVVGDDAQSIYAFRGATVRN
ILDFPAQFTPAAHQVTLSQNYRSTQPILHAANAVIGLAAERYTKDLWSERTSAEKPGVVVVNDEADQARFVVERVLERRE
AGLSLMSQAVLFRAADHSAQVEIELARRNIPFVKFGGLKFLESTHVKDAMAVVRWLENPLDRIAGFRTLQLLPGIGPKTA
ARVLDALAVATEPLFALESFEPPPAAAPAWTDLLTLARTLLGPASPWPSEFEQVVAWYQPHLERIHDDAAARMADLQQLE
RIAATYASRERFLTELTLDPPSASSDESGVPLRDEDFLILSTIHSAKGQEWKAVYVLNAVDGCMPSDLATGTTEEIEEER
RLLYVAMTRAKDHLDVVVPQRFYVHQQPGFGDRHVYASRTRFLPNRVMPNFYSRSWPPPPMPGEAPSKATLAPVDLASRM
RNMWR

Sequences:

>Translated_725_residues
MDIQFAPVSSALRPGASEPPVSLDLAPLAERPSAPAAVVSPDADVPAYLSRLNPEQRAAVEHHSDGPLLIIAGAGSGKTN
TLAHRVAHLVLNGADPRRILLLTFSRRAAAEMGRRVERIVDQALGTNTGAGRAALQWSGTFHAIGARLLREYAETLGLSP
AFTICDRGDAADLMHVVRHDLGLSEQASRFPKKETCLSIYSRVVNTQAPLEDVLKHWFPRYTMWVDALRTLFAGYVEAKQ
KQQVLDYDDLLLYWAQALTEPAIAQDMGARFDHILVDEYQDTNALQASILLALRPDGRGLTVVGDDAQSIYAFRGATVRN
ILDFPAQFTPAAHQVTLSQNYRSTQPILHAANAVIGLAAERYTKDLWSERTSAEKPGVVVVNDEADQARFVVERVLERRE
AGLSLMSQAVLFRAADHSAQVEIELARRNIPFVKFGGLKFLESTHVKDAMAVVRWLENPLDRIAGFRTLQLLPGIGPKTA
ARVLDALAVATEPLFALESFEPPPAAAPAWTDLLTLARTLLGPASPWPSEFEQVVAWYQPHLERIHDDAAARMADLQQLE
RIAATYASRERFLTELTLDPPSASSDESGVPLRDEDFLILSTIHSAKGQEWKAVYVLNAVDGCMPSDLATGTTEEIEEER
RLLYVAMTRAKDHLDVVVPQRFYVHQQPGFGDRHVYASRTRFLPNRVMPNFYSRSWPPPPMPGEAPSKATLAPVDLASRM
RNMWR
>Mature_725_residues
MDIQFAPVSSALRPGASEPPVSLDLAPLAERPSAPAAVVSPDADVPAYLSRLNPEQRAAVEHHSDGPLLIIAGAGSGKTN
TLAHRVAHLVLNGADPRRILLLTFSRRAAAEMGRRVERIVDQALGTNTGAGRAALQWSGTFHAIGARLLREYAETLGLSP
AFTICDRGDAADLMHVVRHDLGLSEQASRFPKKETCLSIYSRVVNTQAPLEDVLKHWFPRYTMWVDALRTLFAGYVEAKQ
KQQVLDYDDLLLYWAQALTEPAIAQDMGARFDHILVDEYQDTNALQASILLALRPDGRGLTVVGDDAQSIYAFRGATVRN
ILDFPAQFTPAAHQVTLSQNYRSTQPILHAANAVIGLAAERYTKDLWSERTSAEKPGVVVVNDEADQARFVVERVLERRE
AGLSLMSQAVLFRAADHSAQVEIELARRNIPFVKFGGLKFLESTHVKDAMAVVRWLENPLDRIAGFRTLQLLPGIGPKTA
ARVLDALAVATEPLFALESFEPPPAAAPAWTDLLTLARTLLGPASPWPSEFEQVVAWYQPHLERIHDDAAARMADLQQLE
RIAATYASRERFLTELTLDPPSASSDESGVPLRDEDFLILSTIHSAKGQEWKAVYVLNAVDGCMPSDLATGTTEEIEEER
RLLYVAMTRAKDHLDVVVPQRFYVHQQPGFGDRHVYASRTRFLPNRVMPNFYSRSWPPPPMPGEAPSKATLAPVDLASRM
RNMWR

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=675, Percent_Identity=31.4074074074074, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI48994965, Length=645, Percent_Identity=29.6124031007752, Blast_Score=186, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6322369, Length=472, Percent_Identity=27.7542372881356, Blast_Score=141, Evalue=3e-34,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 80116; Mature: 80116

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIQFAPVSSALRPGASEPPVSLDLAPLAERPSAPAAVVSPDADVPAYLSRLNPEQRAAV
CCCCCCCCHHHHCCCCCCCCCEEECCCCCCCCCCCEEEECCCCCHHHHHHHCCHHHHHHH
EHHSDGPLLIIAGAGSGKTNTLAHRVAHLVLNGADPRRILLLTFSRRAAAEMGRRVERIV
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHH
DQALGTNTGAGRAALQWSGTFHAIGARLLREYAETLGLSPAFTICDRGDAADLMHVVRHD
HHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHH
LGLSEQASRFPKKETCLSIYSRVVNTQAPLEDVLKHWFPRYTMWVDALRTLFAGYVEAKQ
CCCCHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KQQVLDYDDLLLYWAQALTEPAIAQDMGARFDHILVDEYQDTNALQASILLALRPDGRGL
HHHHCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCHHEEEEEEEECCCCCEE
TVVGDDAQSIYAFRGATVRNILDFPAQFTPAAHQVTLSQNYRSTQPILHAANAVIGLAAE
EEECCCHHHHHHHCCCHHHHHHHCCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHH
RYTKDLWSERTSAEKPGVVVVNDEADQARFVVERVLERREAGLSLMSQAVLFRAADHSAQ
HHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VEIELARRNIPFVKFGGLKFLESTHVKDAMAVVRWLENPLDRIAGFRTLQLLPGIGPKTA
EEEEEHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHH
ARVLDALAVATEPLFALESFEPPPAAAPAWTDLLTLARTLLGPASPWPSEFEQVVAWYQP
HHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
HLERIHDDAAARMADLQQLERIAATYASRERFLTELTLDPPSASSDESGVPLRDEDFLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEE
STIHSAKGQEWKAVYVLNAVDGCMPSDLATGTTEEIEEERRLLYVAMTRAKDHLDVVVPQ
EEHHHCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHEEEEEEHHCCCCCEEEECC
RFYVHQQPGFGDRHVYASRTRFLPNRVMPNFYSRSWPPPPMPGEAPSKATLAPVDLASRM
CEEEECCCCCCCCCCHHHHHHHCCHHCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
RNMWR
HHHCC
>Mature Secondary Structure
MDIQFAPVSSALRPGASEPPVSLDLAPLAERPSAPAAVVSPDADVPAYLSRLNPEQRAAV
CCCCCCCCHHHHCCCCCCCCCEEECCCCCCCCCCCEEEECCCCCHHHHHHHCCHHHHHHH
EHHSDGPLLIIAGAGSGKTNTLAHRVAHLVLNGADPRRILLLTFSRRAAAEMGRRVERIV
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHH
DQALGTNTGAGRAALQWSGTFHAIGARLLREYAETLGLSPAFTICDRGDAADLMHVVRHD
HHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHH
LGLSEQASRFPKKETCLSIYSRVVNTQAPLEDVLKHWFPRYTMWVDALRTLFAGYVEAKQ
CCCCHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KQQVLDYDDLLLYWAQALTEPAIAQDMGARFDHILVDEYQDTNALQASILLALRPDGRGL
HHHHCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCHHEEEEEEEECCCCCEE
TVVGDDAQSIYAFRGATVRNILDFPAQFTPAAHQVTLSQNYRSTQPILHAANAVIGLAAE
EEECCCHHHHHHHCCCHHHHHHHCCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHH
RYTKDLWSERTSAEKPGVVVVNDEADQARFVVERVLERREAGLSLMSQAVLFRAADHSAQ
HHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VEIELARRNIPFVKFGGLKFLESTHVKDAMAVVRWLENPLDRIAGFRTLQLLPGIGPKTA
EEEEEHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHH
ARVLDALAVATEPLFALESFEPPPAAAPAWTDLLTLARTLLGPASPWPSEFEQVVAWYQP
HHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
HLERIHDDAAARMADLQQLERIAATYASRERFLTELTLDPPSASSDESGVPLRDEDFLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEE
STIHSAKGQEWKAVYVLNAVDGCMPSDLATGTTEEIEEERRLLYVAMTRAKDHLDVVVPQ
EEHHHCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHEEEEEEHHCCCCCEEEECC
RFYVHQQPGFGDRHVYASRTRFLPNRVMPNFYSRSWPPPPMPGEAPSKATLAPVDLASRM
CEEEECCCCCCCCCCHHHHHHHCCHHCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
RNMWR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]