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The map label for this gene is mhpB

Identifier: 73539977

GI number: 73539977

Start: 301910

End: 302854

Strand: Direct

Name: mhpB

Synonym: Reut_A0271

Alternate gene names: 73539977

Gene position: 301910-302854 (Clockwise)

Preceding gene: 73539976

Following gene: 73539978

Centisome position: 7.93

GC content: 67.51

Gene sequence:

>945_bases
ATGCCGATCCAACTGGAATGTCTGTCGCATACGCCGTTGCACGGCTACGTCGACCCCGCGCCCGAAGTCGTCGCCGAGGT
CGAGCGCGTACAGGCTGCGGCGCGCGATCGCGTGCGTGCATTCGATCCCGAACTGGTCGTGGTCTTTGCGCCGGACCACT
TCAACGGCTTCTTCTATGACGTGATGCCGCCGTTCTGCATCGGCGCAGCCGCCACCGCGATCGGTGACTTCAAGAGCCTG
GCCGGCAAGCTGCCTGTGCCGGCGGACCTCGCTCTGTCGCTGGCCGAAAGCGTGATGGCCGCCGATATCGATGTCGCGCT
GTCGCACCGCATGCAGGTCGACCATGGCTGCGCGGATGCGCTCGCGGCATTGACCGGCAGCCTGCATCGCTATCCGGTCA
TCCCCGTGTTCATCAATTCCGTGGCGCCGCCGATGGCGACACTGCGCCGCGCGCGCCTGCTCGGCGATGCGGTCGGCCGC
TTCCTGTCGCGTGCCGGCAAGCGTGTGCTGGTGGTCGGTTCTGGCGGCATCTCGCATGAGCCGCCGGTGCCGGAGTTGGC
CGGCGCCAGCGAGGAAGTTGCCGAACGCCTGATCGCAGGACGCAACCCGTCGCCCGAATCGCGCGCCGCGCGCCAGGCCC
GCACTGTTGCCGCCGCGAAGTCTTTCGTCGCGGGCGACAGCCACCTGCATCCGCTCAATCCCGAGTGGGACCGCGCATTC
CTGTCGCTGCTCGCTTCCGGTGAACTCACCGCCGTCGATGGCATGACCAACGACGCGATCACGCGCGACGGCGGCAAGTC
GGCGCACGAGATTCGCACGTGGGTGGCTGCATTCGGTGCGCTGGCTGCGTACGGGCCGTATCGCGCATCGCTCGATTTCT
ACCGCGCGATTCCTGAGTGGATCGCGGGCTTCGCCACCATGCATGCCGAGCCTGCAGCGGTCTGA

Upstream 100 bases:

>100_bases
TGACGGAGGTGCCGCCTTATGCCGAGGCCTCTACCCCTTCCAAGACGTCGCGCCCCGTGCTCGACCCCGTCACCGTTACC
CAGGCAGCAGGAGCATGAAG

Downstream 100 bases:

>100_bases
CCCGATCAAGGAGAACCCCGATGACTGAACCAAATTTCGTCAACGCCATCGCAGCACGCCTGCGCGATGCCGAGGCCACG
CGCCAGACCGTCGCGCCGGT

Product: 3-(2,3-dihydroxyphenyl)propionate dioxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MPIQLECLSHTPLHGYVDPAPEVVAEVERVQAAARDRVRAFDPELVVVFAPDHFNGFFYDVMPPFCIGAAATAIGDFKSL
AGKLPVPADLALSLAESVMAADIDVALSHRMQVDHGCADALAALTGSLHRYPVIPVFINSVAPPMATLRRARLLGDAVGR
FLSRAGKRVLVVGSGGISHEPPVPELAGASEEVAERLIAGRNPSPESRAARQARTVAAAKSFVAGDSHLHPLNPEWDRAF
LSLLASGELTAVDGMTNDAITRDGGKSAHEIRTWVAAFGALAAYGPYRASLDFYRAIPEWIAGFATMHAEPAAV

Sequences:

>Translated_314_residues
MPIQLECLSHTPLHGYVDPAPEVVAEVERVQAAARDRVRAFDPELVVVFAPDHFNGFFYDVMPPFCIGAAATAIGDFKSL
AGKLPVPADLALSLAESVMAADIDVALSHRMQVDHGCADALAALTGSLHRYPVIPVFINSVAPPMATLRRARLLGDAVGR
FLSRAGKRVLVVGSGGISHEPPVPELAGASEEVAERLIAGRNPSPESRAARQARTVAAAKSFVAGDSHLHPLNPEWDRAF
LSLLASGELTAVDGMTNDAITRDGGKSAHEIRTWVAAFGALAAYGPYRASLDFYRAIPEWIAGFATMHAEPAAV
>Mature_313_residues
PIQLECLSHTPLHGYVDPAPEVVAEVERVQAAARDRVRAFDPELVVVFAPDHFNGFFYDVMPPFCIGAAATAIGDFKSLA
GKLPVPADLALSLAESVMAADIDVALSHRMQVDHGCADALAALTGSLHRYPVIPVFINSVAPPMATLRRARLLGDAVGRF
LSRAGKRVLVVGSGGISHEPPVPELAGASEEVAERLIAGRNPSPESRAARQARTVAAAKSFVAGDSHLHPLNPEWDRAFL
SLLASGELTAVDGMTNDAITRDGGKSAHEIRTWVAAFGALAAYGPYRASLDFYRAIPEWIAGFATMHAEPAAV

Specific function: Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3- dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2- hydroxy-6-ketononatrienedioate, respectively

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ligB/mhpB extradiol dioxygenase family

Homologues:

Organism=Escherichia coli, GI1786544, Length=310, Percent_Identity=55.1612903225806, Blast_Score=355, Evalue=2e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MHPB_CUPPJ (Q476N0)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294497.1
- GeneID:   3610127
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0271
- NMPDR:   fig|264198.3.peg.783
- HOGENOM:   HBG565586
- OMA:   PLNPEWD
- ProtClustDB:   PRK13370
- BioCyc:   REUT264198:REUT_A0271-MONOMER
- HAMAP:   MF_01653
- InterPro:   IPR004183

Pfam domain/function: PF02900 LigB; SSF53213 Xdiol_dOase_3B

EC number: =1.13.11.16

Molecular weight: Translated: 33299; Mature: 33168

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: ACT_SITE 115-115 ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIQLECLSHTPLHGYVDPAPEVVAEVERVQAAARDRVRAFDPELVVVFAPDHFNGFFYD
CCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEHH
VMPPFCIGAAATAIGDFKSLAGKLPVPADLALSLAESVMAADIDVALSHRMQVDHGCADA
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LAALTGSLHRYPVIPVFINSVAPPMATLRRARLLGDAVGRFLSRAGKRVLVVGSGGISHE
HHHHHCCHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
PPVPELAGASEEVAERLIAGRNPSPESRAARQARTVAAAKSFVAGDSHLHPLNPEWDRAF
CCCCHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LSLLASGELTAVDGMTNDAITRDGGKSAHEIRTWVAAFGALAAYGPYRASLDFYRAIPEW
HHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IAGFATMHAEPAAV
HHHHHHHCCCCCCC
>Mature Secondary Structure 
PIQLECLSHTPLHGYVDPAPEVVAEVERVQAAARDRVRAFDPELVVVFAPDHFNGFFYD
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEHH
VMPPFCIGAAATAIGDFKSLAGKLPVPADLALSLAESVMAADIDVALSHRMQVDHGCADA
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LAALTGSLHRYPVIPVFINSVAPPMATLRRARLLGDAVGRFLSRAGKRVLVVGSGGISHE
HHHHHCCHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
PPVPELAGASEEVAERLIAGRNPSPESRAARQARTVAAAKSFVAGDSHLHPLNPEWDRAF
CCCCHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LSLLASGELTAVDGMTNDAITRDGGKSAHEIRTWVAAFGALAAYGPYRASLDFYRAIPEW
HHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IAGFATMHAEPAAV
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA