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The map label for this gene is mhpD [H]

Identifier: 73539978

GI number: 73539978

Start: 302875

End: 303663

Strand: Direct

Name: mhpD [H]

Synonym: Reut_A0272

Alternate gene names: 73539978

Gene position: 302875-303663 (Clockwise)

Preceding gene: 73539977

Following gene: 73539979

Centisome position: 7.96

GC content: 67.55

Gene sequence:

>789_bases
ATGACTGAACCAAATTTCGTCAACGCCATCGCAGCACGCCTGCGCGATGCCGAGGCCACGCGCCAGACCGTCGCGCCGGT
GCGTGGCGAGATCGCGATCGATGACATCGCCACCGCGTATGCCGTGCAGCAGGCCAACGTCGACGCGCGCCTCGCACAAG
GCGAACGCATCGTCGGCCGCAAGATCGGCCTGACTTCGCTGGCCGTGCAGAAGCAGCTCGGCGTCGACCAGCCCGACTTC
GGCGCGCTGTTCGCGAGCATGGCGTATGGCGACAGCCAGCCGATGCCGCTGTCGCGCCTGATCCAGCCGAAGGTCGAAGC
CGAGATCGCACTGGTGCTCGAGCACGACCTGACGCACGAGCGTCATACGTTTGCCGACATCCTGCGCGCGAGCGCCTACG
CGCTGGCGGCAATCGAGGTCGTCGACAGCCGCATCCAGAACTGGGACATCCGCTTTGTCGATACCGTCGCCGACAACGCG
TCGAGCGCGCTGTTCGTGCTCGGTAGCCGTCCGGTGCCGCTGTCCGCCGTCGATCTCGCTGCATGCGCGATGACGCTGTC
GCGCGACGGAGAAGTGCTGTCGCGCGGCAACGGTGCGGCCTGCCTCGGCAACCCGCTCAATGCGGCGGTCTGGCTTGCGG
ATCGCATGGTGCAGCTCGGCACGCCGCTGCGTGCTGGAGACGTGGTGCTGACCGGTGCGCTCGGCCCGATGGTCGCCGTG
ACCGAGCCCGGCACCTACACCACCGAAATCGAAGGACTCGGCAGCGTCCGCGCGACGTTCGCCGCATAA

Upstream 100 bases:

>100_bases
CATCGCTCGATTTCTACCGCGCGATTCCTGAGTGGATCGCGGGCTTCGCCACCATGCATGCCGAGCCTGCAGCGGTCTGA
CCCGATCAAGGAGAACCCCG

Downstream 100 bases:

>100_bases
GCCGTCACCAAGAGCGGCACACACAGACAAAAGCGGAGACAAGAATGCAACCATCAAACAGCAGCGGCGCTAACGGGCGC
AGCACAACAAGCAGCATCAC

Product: 4-oxalocrotonate decarboxylase

Products: NA

Alternate protein names: 2-hydroxypentadienoic acid hydratase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTEPNFVNAIAARLRDAEATRQTVAPVRGEIAIDDIATAYAVQQANVDARLAQGERIVGRKIGLTSLAVQKQLGVDQPDF
GALFASMAYGDSQPMPLSRLIQPKVEAEIALVLEHDLTHERHTFADILRASAYALAAIEVVDSRIQNWDIRFVDTVADNA
SSALFVLGSRPVPLSAVDLAACAMTLSRDGEVLSRGNGAACLGNPLNAAVWLADRMVQLGTPLRAGDVVLTGALGPMVAV
TEPGTYTTEIEGLGSVRATFAA

Sequences:

>Translated_262_residues
MTEPNFVNAIAARLRDAEATRQTVAPVRGEIAIDDIATAYAVQQANVDARLAQGERIVGRKIGLTSLAVQKQLGVDQPDF
GALFASMAYGDSQPMPLSRLIQPKVEAEIALVLEHDLTHERHTFADILRASAYALAAIEVVDSRIQNWDIRFVDTVADNA
SSALFVLGSRPVPLSAVDLAACAMTLSRDGEVLSRGNGAACLGNPLNAAVWLADRMVQLGTPLRAGDVVLTGALGPMVAV
TEPGTYTTEIEGLGSVRATFAA
>Mature_261_residues
TEPNFVNAIAARLRDAEATRQTVAPVRGEIAIDDIATAYAVQQANVDARLAQGERIVGRKIGLTSLAVQKQLGVDQPDFG
ALFASMAYGDSQPMPLSRLIQPKVEAEIALVLEHDLTHERHTFADILRASAYALAAIEVVDSRIQNWDIRFVDTVADNAS
SALFVLGSRPVPLSAVDLAACAMTLSRDGEVLSRGNGAACLGNPLNAAVWLADRMVQLGTPLRAGDVVLTGALGPMVAVT
EPGTYTTEIEGLGSVRATFAA

Specific function: Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family. MhpD subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=253, Percent_Identity=54.1501976284585, Blast_Score=278, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =4.2.1.80 [H]

Molecular weight: Translated: 27692; Mature: 27561

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPNFVNAIAARLRDAEATRQTVAPVRGEIAIDDIATAYAVQQANVDARLAQGERIVGR
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCHHHHCCCEEHHH
KIGLTSLAVQKQLGVDQPDFGALFASMAYGDSQPMPLSRLIQPKVEAEIALVLEHDLTHE
HCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEEECCCCCH
RHTFADILRASAYALAAIEVVDSRIQNWDIRFVDTVADNASSALFVLGSRPVPLSAVDLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHH
ACAMTLSRDGEVLSRGNGAACLGNPLNAAVWLADRMVQLGTPLRAGDVVLTGALGPMVAV
HHHHHHCCCCCEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEE
TEPGTYTTEIEGLGSVRATFAA
ECCCCEEEEECCCCCCEEEECC
>Mature Secondary Structure 
TEPNFVNAIAARLRDAEATRQTVAPVRGEIAIDDIATAYAVQQANVDARLAQGERIVGR
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCHHHHCCCEEHHH
KIGLTSLAVQKQLGVDQPDFGALFASMAYGDSQPMPLSRLIQPKVEAEIALVLEHDLTHE
HCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEEECCCCCH
RHTFADILRASAYALAAIEVVDSRIQNWDIRFVDTVADNASSALFVLGSRPVPLSAVDLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHH
ACAMTLSRDGEVLSRGNGAACLGNPLNAAVWLADRMVQLGTPLRAGDVVLTGALGPMVAV
HHHHHHCCCCCEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEE
TEPGTYTTEIEGLGSVRATFAA
ECCCCEEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA