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The map label for this gene is mhpA

Identifier: 73539976

GI number: 73539976

Start: 300051

End: 301907

Strand: Direct

Name: mhpA

Synonym: Reut_A0270

Alternate gene names: 73539976

Gene position: 300051-301907 (Clockwise)

Preceding gene: 73539972

Following gene: 73539977

Centisome position: 7.88

GC content: 67.04

Gene sequence:

>1857_bases
ATGAACACCACGGCCACGCAGACGGTACACGCCGACCGTCTTCCCGATGTCGATACCGACGTCGCCATCATCGGCGCGGG
CCCGGTCGGGCTGATGATCGCCAACATTCTCGGCCTGCAGGGCGTGCGCGTCACGGTCGTCGAAAAGCTGGACCAACTGA
TCGACTATCCGCGCGCCATCGGCCTCGACGACGAAGCGCTGCGCGTGTTCCAGGCCATCGGGCTTGCCGACACGCTGCTG
CCGCACACCACGCCTGACCACTGGATGCGCTTCGTCACGAAGGACGGCCATTGCTTCGCGTCCATCGAGCCGCGCACCGA
CGAGTTCGGCTGGTCGCGCCGCAATGCGTTCATCCAGCCGTTGGCCGACCGCGTGCTGTTCGAGGGCCTGCAGCGCTTCG
AGCACGTGCAGGTGCTGTTCGGCCACGGTGTCGAGGCGTTCACGCAGGACGGCCACGGCGTCAGTGTCGAGGTGACCACC
AGCGCCGGCCGGCGCCGCACGATCCGCGCCGCGTACATGGTCGGCGCGGACGGCGGCAACAGCATTGTGCGCCGCGCGCT
GCAGGTGCCGTTCGAAGGGCGTACCAAGCCGAACCAGTGGATCGTCGTCGACGTGCGCAACGACCCGGTCGGTTCGCCGC
ACATCTACATGCACTGCGATCACGAGCGCCCCTATGTTTCGGCCGCGCTGCCGCACGGTATCCGCCGCTTCGAGTTCATG
GTGATGCCGGGCGAGACCGAAGAGGAGCTGTCGAAGCCCGAGAACATGGCCGCGCTGATCCGCAAGGTGGTGGCCGATCC
GGACAAGGTCGACTACATCCGCAAGCGCGTGTACACACACAACGCCCGGCTCGCCTCGACGTTCCGCGTCGATCGCGTGC
TGCTGGCTGGCGACGCCGCGCACATCATGCCGGTGTGGCAAGGGCAGGGCTACAACAGCGGCATCCGCGACGCCAACAAC
CTAGGCTGGAAGCTCGCGATGGTCGCGAAGGGCCTTGCCGATGGCCGGCTGCTCGACACCTACACAGCCGAACGCCGTGC
GCATGCGCGTTCGATGATCCACCTGTCCGAAGTGGCAGGCGACATCTTCGCACCGAACACGCGCTTCGGCGTGCGCTTCC
GCGACGCCTTCGTGCGCAGCTTCAACGTGTTGCCCGCGGTCAAGCGCTACTTCATCGAGATGCGGTTCAAGCCGATGCCG
CGCTATGAGGAAGGCGTGGTGCTGCTGCCGCCGCCGCAACATCGCGGCGGCTGGCTCGCCCGCATGCTGGAACGCGCCGG
CAACTCGGCACCGGGACGCCTGCTTGGCCTGATGAGCGAGAAACGCGAGTCGTTCCTCGGTCGCCTGGTGTATGGCAAGG
ATCCGCTGGCCGGCTCGCCCGTGGGCCGCATGTTCATCCAGCCGCGCGTACGTACTGCCGATGATCGCCTCGCGCTGCTC
GACGACGCCATCGGCAATGGTTTTGCCGTGATCGGCTGGGGCACCAATCCGACCTTCGGCCTGACGCCGCAGGCACGCGC
GCTCGCCGAGAAGGCCGGCATGCGCTTCGTACTCGCCAAGCCTGATGTGCAACTGCGTCACGGCGACGACGTGCCCGATG
GGGTGATCGCGATCGGCGATGCAAGCGGCCGCCTGAAGGAATGGTTCAGCCGCCTGCCGCAGTCGGTGGTCCTGCTGCGT
CCGGATCGCTTCGTTGCGGGCGTGTGCTCGCCCCAGCAGGTGTCGGAGGCCATCACTGAACTCGCCGGCAAGCTCGGCAT
GACGGAGGTGCCGCCTTATGCCGAGGCCTCTACCCCTTCCAAGACGTCGCGCCCCGTGCTCGACCCCGTCACCGTTACCC
AGGCAGCAGGAGCATGA

Upstream 100 bases:

>100_bases
GCCGGCATCGCCGGCCACCTGATTCCAATTCATCGCAGCAGAGCCAATACGCGCCACAGCGGCGCGGGGGCGCGCTCGTG
CCTTGCACAGTAGGAGACCT

Downstream 100 bases:

>100_bases
AGATGCCGATCCAACTGGAATGTCTGTCGCATACGCCGTTGCACGGCTACGTCGACCCCGCGCCCGAAGTCGTCGCCGAG
GTCGAGCGCGTACAGGCTGC

Product: 3-(3-hydroxyphenyl)propionate hydroxylase

Products: NA

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MNTTATQTVHADRLPDVDTDVAIIGAGPVGLMIANILGLQGVRVTVVEKLDQLIDYPRAIGLDDEALRVFQAIGLADTLL
PHTTPDHWMRFVTKDGHCFASIEPRTDEFGWSRRNAFIQPLADRVLFEGLQRFEHVQVLFGHGVEAFTQDGHGVSVEVTT
SAGRRRTIRAAYMVGADGGNSIVRRALQVPFEGRTKPNQWIVVDVRNDPVGSPHIYMHCDHERPYVSAALPHGIRRFEFM
VMPGETEEELSKPENMAALIRKVVADPDKVDYIRKRVYTHNARLASTFRVDRVLLAGDAAHIMPVWQGQGYNSGIRDANN
LGWKLAMVAKGLADGRLLDTYTAERRAHARSMIHLSEVAGDIFAPNTRFGVRFRDAFVRSFNVLPAVKRYFIEMRFKPMP
RYEEGVVLLPPPQHRGGWLARMLERAGNSAPGRLLGLMSEKRESFLGRLVYGKDPLAGSPVGRMFIQPRVRTADDRLALL
DDAIGNGFAVIGWGTNPTFGLTPQARALAEKAGMRFVLAKPDVQLRHGDDVPDGVIAIGDASGRLKEWFSRLPQSVVLLR
PDRFVAGVCSPQQVSEAITELAGKLGMTEVPPYAEASTPSKTSRPVLDPVTVTQAAGA

Sequences:

>Translated_618_residues
MNTTATQTVHADRLPDVDTDVAIIGAGPVGLMIANILGLQGVRVTVVEKLDQLIDYPRAIGLDDEALRVFQAIGLADTLL
PHTTPDHWMRFVTKDGHCFASIEPRTDEFGWSRRNAFIQPLADRVLFEGLQRFEHVQVLFGHGVEAFTQDGHGVSVEVTT
SAGRRRTIRAAYMVGADGGNSIVRRALQVPFEGRTKPNQWIVVDVRNDPVGSPHIYMHCDHERPYVSAALPHGIRRFEFM
VMPGETEEELSKPENMAALIRKVVADPDKVDYIRKRVYTHNARLASTFRVDRVLLAGDAAHIMPVWQGQGYNSGIRDANN
LGWKLAMVAKGLADGRLLDTYTAERRAHARSMIHLSEVAGDIFAPNTRFGVRFRDAFVRSFNVLPAVKRYFIEMRFKPMP
RYEEGVVLLPPPQHRGGWLARMLERAGNSAPGRLLGLMSEKRESFLGRLVYGKDPLAGSPVGRMFIQPRVRTADDRLALL
DDAIGNGFAVIGWGTNPTFGLTPQARALAEKAGMRFVLAKPDVQLRHGDDVPDGVIAIGDASGRLKEWFSRLPQSVVLLR
PDRFVAGVCSPQQVSEAITELAGKLGMTEVPPYAEASTPSKTSRPVLDPVTVTQAAGA
>Mature_618_residues
MNTTATQTVHADRLPDVDTDVAIIGAGPVGLMIANILGLQGVRVTVVEKLDQLIDYPRAIGLDDEALRVFQAIGLADTLL
PHTTPDHWMRFVTKDGHCFASIEPRTDEFGWSRRNAFIQPLADRVLFEGLQRFEHVQVLFGHGVEAFTQDGHGVSVEVTT
SAGRRRTIRAAYMVGADGGNSIVRRALQVPFEGRTKPNQWIVVDVRNDPVGSPHIYMHCDHERPYVSAALPHGIRRFEFM
VMPGETEEELSKPENMAALIRKVVADPDKVDYIRKRVYTHNARLASTFRVDRVLLAGDAAHIMPVWQGQGYNSGIRDANN
LGWKLAMVAKGLADGRLLDTYTAERRAHARSMIHLSEVAGDIFAPNTRFGVRFRDAFVRSFNVLPAVKRYFIEMRFKPMP
RYEEGVVLLPPPQHRGGWLARMLERAGNSAPGRLLGLMSEKRESFLGRLVYGKDPLAGSPVGRMFIQPRVRTADDRLALL
DDAIGNGFAVIGWGTNPTFGLTPQARALAEKAGMRFVLAKPDVQLRHGDDVPDGVIAIGDASGRLKEWFSRLPQSVVLLR
PDRFVAGVCSPQQVSEAITELAGKLGMTEVPPYAEASTPSKTSRPVLDPVTVTQAAGA

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI)

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family

Homologues:

Organism=Escherichia coli, GI1786543, Length=581, Percent_Identity=53.184165232358, Blast_Score=621, Evalue=1e-179,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MHPA_CUPPJ (Q476N1)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294496.1
- ProteinModelPortal:   Q476N1
- SMR:   Q476N1
- GeneID:   3610126
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0270
- NMPDR:   fig|264198.3.peg.782
- HOGENOM:   HBG565573
- OMA:   TTSSTRW
- ProtClustDB:   PRK06183
- BioCyc:   REUT264198:REUT_A0270-MONOMER
- HAMAP:   MF_01652
- InterPro:   IPR002938
- InterPro:   IPR003042
- PRINTS:   PR00420

Pfam domain/function: PF01494 FAD_binding_3

EC number: 1.14.13.-

Molecular weight: Translated: 68162; Mature: 68162

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTATQTVHADRLPDVDTDVAIIGAGPVGLMIANILGLQGVRVTVVEKLDQLIDYPRAI
CCCCCCCEEECCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHC
GLDDEALRVFQAIGLADTLLPHTTPDHWMRFVTKDGHCFASIEPRTDEFGWSRRNAFIQP
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHH
LADRVLFEGLQRFEHVQVLFGHGVEAFTQDGHGVSVEVTTSAGRRRTIRAAYMVGADGGN
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEECCCCCCEEEEEEEEEECCCCH
SIVRRALQVPFEGRTKPNQWIVVDVRNDPVGSPHIYMHCDHERPYVSAALPHGIRRFEFM
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCEEEHHCCCCCEEEEEE
VMPGETEEELSKPENMAALIRKVVADPDKVDYIRKRVYTHNARLASTFRVDRVLLAGDAA
EECCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHEEEEEECCCH
HIMPVWQGQGYNSGIRDANNLGWKLAMVAKGLADGRLLDTYTAERRAHARSMIHLSEVAG
HEEEEECCCCCCCCCCCCCCCCEEEEHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCC
DIFAPNTRFGVRFRDAFVRSFNVLPAVKRYFIEMRFKPMPRYEEGVVLLPPPQHRGGWLA
HHCCCCCCCCEEHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCHHH
RMLERAGNSAPGRLLGLMSEKRESFLGRLVYGKDPLAGSPVGRMFIQPRVRTADDRLALL
HHHHHCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEECCCCCCCCCHHHHH
DDAIGNGFAVIGWGTNPTFGLTPQARALAEKAGMRFVLAKPDVQLRHGDDVPDGVIAIGD
HHHCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCEEEECCCCCCCCEEEEEC
ASGRLKEWFSRLPQSVVLLRPDRFVAGVCSPQQVSEAITELAGKLGMTEVPPYAEASTPS
CCCHHHHHHHHCCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KTSRPVLDPVTVTQAAGA
CCCCCCCCCEEHHCCCCC
>Mature Secondary Structure
MNTTATQTVHADRLPDVDTDVAIIGAGPVGLMIANILGLQGVRVTVVEKLDQLIDYPRAI
CCCCCCCEEECCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHC
GLDDEALRVFQAIGLADTLLPHTTPDHWMRFVTKDGHCFASIEPRTDEFGWSRRNAFIQP
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHH
LADRVLFEGLQRFEHVQVLFGHGVEAFTQDGHGVSVEVTTSAGRRRTIRAAYMVGADGGN
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEECCCCCCEEEEEEEEEECCCCH
SIVRRALQVPFEGRTKPNQWIVVDVRNDPVGSPHIYMHCDHERPYVSAALPHGIRRFEFM
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCEEEHHCCCCCEEEEEE
VMPGETEEELSKPENMAALIRKVVADPDKVDYIRKRVYTHNARLASTFRVDRVLLAGDAA
EECCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHEEEEEECCCH
HIMPVWQGQGYNSGIRDANNLGWKLAMVAKGLADGRLLDTYTAERRAHARSMIHLSEVAG
HEEEEECCCCCCCCCCCCCCCCEEEEHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCC
DIFAPNTRFGVRFRDAFVRSFNVLPAVKRYFIEMRFKPMPRYEEGVVLLPPPQHRGGWLA
HHCCCCCCCCEEHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCHHH
RMLERAGNSAPGRLLGLMSEKRESFLGRLVYGKDPLAGSPVGRMFIQPRVRTADDRLALL
HHHHHCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEECCCCCCCCCHHHHH
DDAIGNGFAVIGWGTNPTFGLTPQARALAEKAGMRFVLAKPDVQLRHGDDVPDGVIAIGD
HHHCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCEEEECCCCCCCCEEEEEC
ASGRLKEWFSRLPQSVVLLRPDRFVAGVCSPQQVSEAITELAGKLGMTEVPPYAEASTPS
CCCHHHHHHHHCCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KTSRPVLDPVTVTQAAGA
CCCCCCCCCEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA