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The map label for this gene is bacD [H]

Identifier: 71910276

GI number: 71910276

Start: 453769

End: 455016

Strand: Direct

Name: bacD [H]

Synonym: M5005_Spy_0463

Alternate gene names: 71910276

Gene position: 453769-455016 (Clockwise)

Preceding gene: 71910275

Following gene: 71910277

Centisome position: 24.68

GC content: 29.97

Gene sequence:

>1248_bases
ATGAAAAAGAATATTTTAATTGTAGGTGGAGGTCCAGAGATAACTATATGTTATGATTTAATTAACAATAGTACGGAGTA
TAATTTCTTTTCAGTATCAGAAATTCCTACGATGTATCCAGAGTTGTTTGTAGAAACAATTGAAACCAGCTTTAGAAATT
TTGGAAAGACGATTGAACAAATTAAAGAGCTCAAATATTCTATTGATGCTGTTGCTTGTTGGGATGAAGCTTTAACGCAT
ATAGCTGATGATATTTCAAAAGAATTAGGATTGAACCCTATTTCATCGCTAGATTCTCAATCATTCAGGTTTAAAGATCG
AATGAGAATGGTATGTGAAGCTGGTGGACTAAAAATGCCCAAATATAAAATTATCAATCAATTTTCAGATACAAATAAAA
TAATCAATTGGAAATATCCATTAATAGTGAAACCAACCTCTTTTTTAGCAAGTATAGGAGTGAAAAAAGTTTATAATTTT
TCAGAATTACAACAAGCAGTTTCTCAAATGCTTAATGTAAAATTTCCGGTTTACATAGCTTCAGGGGTATATGAATTGGG
GGAATTATATAATTTAGAACCTAGAGTTTTGGTGGAAGAGTTTATTGATGGAGAAGAATATAGCTTAGAGAGCGTGGTAA
GAAATGGAATCTATACACCTCTAGGGATAACTAAAAAAATAGTGGATGAAAAATTATTTATGGATGAAATAGGCCATATT
TTCCCTTCAAATTTAAACAAAGAAGAAAAAAGTAGAGTATATTCTTGGGCAGAAAAATTACATCAAATTTTGCAATTAAA
TCATATTACGACTCATACAGAATTTAGAATTGGAAGAAATGGAGATATTATTTTAATAGAAATTGGAGCAAGAATCGGTG
GAGATTGTATTCCAAACTTAATGAAAAACAGCTTTATAGTCAAGGGATTTGATTTTTATAATACTTATTTAAAAGCTAGA
CTAGGTTTACAAGAAGAGTTAAATACAGACACTAATAAGTTTTCAGGAATTGTATTTTTCAGACTCAATCCAGAGAATTA
CGGAAAAATATTTAATGGAGTGAGTTACGATGCACCTAAAACTTCACTAATATTTGAAGAAAAGATATATAAAAAGGAAG
GAACTGTATTGCCAAATCCGATTAATTGGAGTAATGAGAGAGTAGGATATGTCTGTTTGGAAAACAAGGAATACGCTGTG
CTAGAAAAGGAGATGAAAGAAATATTAAAATCATTGTCAATAGAGTAA

Upstream 100 bases:

>100_bases
CGTTTAATAGCGTGGATATTGAAGTGCGATGATGTTCGAAAAGTTCAATTAATCAGTAGAGAAAATGGGATTGATTATTT
ACCATAAAGGAGGAAGAAAA

Downstream 100 bases:

>100_bases
ATAGTGAAAGAAACATCAAATCTATAGGTATTTGTTCACCCTCCTATCCGGGGACATTTCATTATCAGAAACGATATAAG
AGAGGAGTAGAATTTTTACA

Product: cytoplasmic protein

Products: NA

Alternate protein names: Bacilysin synthetase; L-amino acid ligase [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKKNILIVGGGPEITICYDLINNSTEYNFFSVSEIPTMYPELFVETIETSFRNFGKTIEQIKELKYSIDAVACWDEALTH
IADDISKELGLNPISSLDSQSFRFKDRMRMVCEAGGLKMPKYKIINQFSDTNKIINWKYPLIVKPTSFLASIGVKKVYNF
SELQQAVSQMLNVKFPVYIASGVYELGELYNLEPRVLVEEFIDGEEYSLESVVRNGIYTPLGITKKIVDEKLFMDEIGHI
FPSNLNKEEKSRVYSWAEKLHQILQLNHITTHTEFRIGRNGDIILIEIGARIGGDCIPNLMKNSFIVKGFDFYNTYLKAR
LGLQEELNTDTNKFSGIVFFRLNPENYGKIFNGVSYDAPKTSLIFEEKIYKKEGTVLPNPINWSNERVGYVCLENKEYAV
LEKEMKEILKSLSIE

Sequences:

>Translated_415_residues
MKKNILIVGGGPEITICYDLINNSTEYNFFSVSEIPTMYPELFVETIETSFRNFGKTIEQIKELKYSIDAVACWDEALTH
IADDISKELGLNPISSLDSQSFRFKDRMRMVCEAGGLKMPKYKIINQFSDTNKIINWKYPLIVKPTSFLASIGVKKVYNF
SELQQAVSQMLNVKFPVYIASGVYELGELYNLEPRVLVEEFIDGEEYSLESVVRNGIYTPLGITKKIVDEKLFMDEIGHI
FPSNLNKEEKSRVYSWAEKLHQILQLNHITTHTEFRIGRNGDIILIEIGARIGGDCIPNLMKNSFIVKGFDFYNTYLKAR
LGLQEELNTDTNKFSGIVFFRLNPENYGKIFNGVSYDAPKTSLIFEEKIYKKEGTVLPNPINWSNERVGYVCLENKEYAV
LEKEMKEILKSLSIE
>Mature_415_residues
MKKNILIVGGGPEITICYDLINNSTEYNFFSVSEIPTMYPELFVETIETSFRNFGKTIEQIKELKYSIDAVACWDEALTH
IADDISKELGLNPISSLDSQSFRFKDRMRMVCEAGGLKMPKYKIINQFSDTNKIINWKYPLIVKPTSFLASIGVKKVYNF
SELQQAVSQMLNVKFPVYIASGVYELGELYNLEPRVLVEEFIDGEEYSLESVVRNGIYTPLGITKKIVDEKLFMDEIGHI
FPSNLNKEEKSRVYSWAEKLHQILQLNHITTHTEFRIGRNGDIILIEIGARIGGDCIPNLMKNSFIVKGFDFYNTYLKAR
LGLQEELNTDTNKFSGIVFFRLNPENYGKIFNGVSYDAPKTSLIFEEKIYKKEGTVLPNPINWSNERVGYVCLENKEYAV
LEKEMKEILKSLSIE

Specific function: Catalyzes the formation of alpha-dipeptides from various L-amino acids in the presence of ATP. Probably catalyzes the ligation of L-alanine and L-anticapsin to produce the final bacilysin antibiotic [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011095 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C [H]

EC number: =6.3.2.28 [H]

Molecular weight: Translated: 47630; Mature: 47630

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50975 ATP_GRASP ; PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNILIVGGGPEITICYDLINNSTEYNFFSVSEIPTMYPELFVETIETSFRNFGKTIEQ
CCCCEEEEECCCCEEEEEEECCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IKELKYSIDAVACWDEALTHIADDISKELGLNPISSLDSQSFRFKDRMRMVCEAGGLKMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCC
KYKIINQFSDTNKIINWKYPLIVKPTSFLASIGVKKVYNFSELQQAVSQMLNVKFPVYIA
HHHHHHHHCCCCCEEEEECCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEH
SGVYELGELYNLEPRVLVEEFIDGEEYSLESVVRNGIYTPLGITKKIVDEKLFMDEIGHI
HHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
FPSNLNKEEKSRVYSWAEKLHQILQLNHITTHTEFRIGRNGDIILIEIGARIGGDCIPNL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCEECCEEEECCCCCEEEEEECCCCCCHHHHHH
MKNSFIVKGFDFYNTYLKARLGLQEELNTDTNKFSGIVFFRLNPENYGKIFNGVSYDAPK
HHCCEEEECHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEECCHHHHHHHCCCCCCCCC
TSLIFEEKIYKKEGTVLPNPINWSNERVGYVCLENKEYAVLEKEMKEILKSLSIE
CHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKNILIVGGGPEITICYDLINNSTEYNFFSVSEIPTMYPELFVETIETSFRNFGKTIEQ
CCCCEEEEECCCCEEEEEEECCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IKELKYSIDAVACWDEALTHIADDISKELGLNPISSLDSQSFRFKDRMRMVCEAGGLKMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCC
KYKIINQFSDTNKIINWKYPLIVKPTSFLASIGVKKVYNFSELQQAVSQMLNVKFPVYIA
HHHHHHHHCCCCCEEEEECCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEH
SGVYELGELYNLEPRVLVEEFIDGEEYSLESVVRNGIYTPLGITKKIVDEKLFMDEIGHI
HHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
FPSNLNKEEKSRVYSWAEKLHQILQLNHITTHTEFRIGRNGDIILIEIGARIGGDCIPNL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCEECCEEEECCCCCEEEEEECCCCCCHHHHHH
MKNSFIVKGFDFYNTYLKARLGLQEELNTDTNKFSGIVFFRLNPENYGKIFNGVSYDAPK
HHCCEEEECHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEECCHHHHHHHCCCCCCCCC
TSLIFEEKIYKKEGTVLPNPINWSNERVGYVCLENKEYAVLEKEMKEILKSLSIE
CHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA