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The map label for this gene is mccF [C]

Identifier: 71910277

GI number: 71910277

Start: 455244

End: 455960

Strand: Direct

Name: mccF [C]

Synonym: M5005_Spy_0464

Alternate gene names: 71910277

Gene position: 455244-455960 (Clockwise)

Preceding gene: 71910276

Following gene: 71910278

Centisome position: 24.76

GC content: 31.94

Gene sequence:

>717_bases
ATGACTTCCATAGGTGGCTACAATTCTAATTCTGTATTAAAGTATATAGACTACGATCTATTTAAACAGAAATCTCCTAT
CTTTATTGGATATTCTGACACAACAGCTCTTGCTTTAGCCTTATATAAAAAGACTGGATGTATAACTTACCTGTCTCAGA
GCGTTATCTCTAATTTTGGAGAGTTTGAACCGTTTAATGAATTAAATTATTTCTATTTTGACTTCATGCTTCAGAGTAAG
TGCGAAACCTTAATGGTACAAATACCAGATGTTTGGACGGATGAGTGGATAAATTGGGAAACCTATGAAAGAACCAAAAA
AACAAATAAAAATGAGTGGATTATTTTTAACAAAGGTGAATTTAATGGTACTTTGATCGGAGGAAATCTCGATACCATTG
TAGGAATAATAGGAACTGAGTATATGCCAAAAATAACAGAGGATACCATACTCTTGTTAGAAGATGTATATACGGATCTA
GGAAGACTATATAGGAATTTCACAACGCTTGCCTTACATGGTATTTTTGATAAAATAGGAGGACTTATTATTTCAAAATT
TGAAACAATAGGGGAAAATAGCGATGTTATTAATGATATCATTAATGAATTTGTAGGGCATAGAAAGATTCCTATTTTAT
TGAATTTTGATTGTGGTCATACCCATCCTAGCTGTTTAATGCCTATTGGTGGTAAAATTACGTTGAGTTTAAGCTAA

Upstream 100 bases:

>100_bases
AAATTTAACAGGAAAAGAAGGTTATAGAGGGGAAATTTCAGGAGGAGTTCAAGACAGAGCTGAAGAATTAAATCAACTTA
TTCCGCGTGTGGATGTCATC

Downstream 100 bases:

>100_bases
AAGAGGTGGTATATGAACAATAGAGAAAGTAAATTAACGGGTTTCTATGATGTTTTTGAGGAAGAAGTAGAAAAAATAAA
TCATATTCATAATGTTTTTG

Product: microcin C7 self-immunity protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTSIGGYNSNSVLKYIDYDLFKQKSPIFIGYSDTTALALALYKKTGCITYLSQSVISNFGEFEPFNELNYFYFDFMLQSK
CETLMVQIPDVWTDEWINWETYERTKKTNKNEWIIFNKGEFNGTLIGGNLDTIVGIIGTEYMPKITEDTILLLEDVYTDL
GRLYRNFTTLALHGIFDKIGGLIISKFETIGENSDVINDIINEFVGHRKIPILLNFDCGHTHPSCLMPIGGKITLSLS

Sequences:

>Translated_238_residues
MTSIGGYNSNSVLKYIDYDLFKQKSPIFIGYSDTTALALALYKKTGCITYLSQSVISNFGEFEPFNELNYFYFDFMLQSK
CETLMVQIPDVWTDEWINWETYERTKKTNKNEWIIFNKGEFNGTLIGGNLDTIVGIIGTEYMPKITEDTILLLEDVYTDL
GRLYRNFTTLALHGIFDKIGGLIISKFETIGENSDVINDIINEFVGHRKIPILLNFDCGHTHPSCLMPIGGKITLSLS
>Mature_237_residues
TSIGGYNSNSVLKYIDYDLFKQKSPIFIGYSDTTALALALYKKTGCITYLSQSVISNFGEFEPFNELNYFYFDFMLQSKC
ETLMVQIPDVWTDEWINWETYERTKKTNKNEWIIFNKGEFNGTLIGGNLDTIVGIIGTEYMPKITEDTILLLEDVYTDLG
RLYRNFTTLALHGIFDKIGGLIISKFETIGENSDVINDIINEFVGHRKIPILLNFDCGHTHPSCLMPIGGKITLSLS

Specific function: L,D-carboxypeptidase [C]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=241, Percent_Identity=29.4605809128631, Blast_Score=77, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 27104; Mature: 26973

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIGGYNSNSVLKYIDYDLFKQKSPIFIGYSDTTALALALYKKTGCITYLSQSVISNFG
CCCCCCCCCCCEEEECCHHHHCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHCC
EFEPFNELNYFYFDFMLQSKCETLMVQIPDVWTDEWINWETYERTKKTNKNEWIIFNKGE
CCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCC
FNGTLIGGNLDTIVGIIGTEYMPKITEDTILLLEDVYTDLGRLYRNFTTLALHGIFDKIG
CCCEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLIISKFETIGENSDVINDIINEFVGHRKIPILLNFDCGHTHPSCLMPIGGKITLSLS
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCEEEEEEC
>Mature Secondary Structure 
TSIGGYNSNSVLKYIDYDLFKQKSPIFIGYSDTTALALALYKKTGCITYLSQSVISNFG
CCCCCCCCCCEEEECCHHHHCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHCC
EFEPFNELNYFYFDFMLQSKCETLMVQIPDVWTDEWINWETYERTKKTNKNEWIIFNKGE
CCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCC
FNGTLIGGNLDTIVGIIGTEYMPKITEDTILLLEDVYTDLGRLYRNFTTLALHGIFDKIG
CCCEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLIISKFETIGENSDVINDIINEFVGHRKIPILLNFDCGHTHPSCLMPIGGKITLSLS
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]