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The map label for this gene is asnS [H]

Identifier: 71910275

GI number: 71910275

Start: 452931

End: 453755

Strand: Direct

Name: asnS [H]

Synonym: M5005_Spy_0462

Alternate gene names: 71910275

Gene position: 452931-453755 (Clockwise)

Preceding gene: 71910274

Following gene: 71910276

Centisome position: 24.64

GC content: 32.48

Gene sequence:

>825_bases
ATGGGGTTGGGGAGTGATTCGCTACCTGTAAAAATTTCCTTATTTGATGTTGATACCTATCTTGCAGATTCGATGCAGTT
TCACTTAGAGTATATACTTAGACTTTTTCCAAAGTCAGGAGTTCATTACTTAATGCCTACTTTTAGAGGAGAAGCAGTAG
ATCATCGACATTTATCGCAATTTTATCATAGTGAGTGTGAGATAGAGGGAGATTTACACTATGTGATGAATTTAGTGGAT
AGATATATTAAGTTTCTGGTTAGTGAAATTCTTAGAGAGTGTCGAGATAGTATTTTTGCTATAACAAAAGATGTAAGTCA
TTTGGAAAGTTTTATTGATCGAAAGATACCAATTATTACGTTTGAAGAGGCTTGTACTATATTGGAAAATGAAATAGATT
CATTGGAATATAATGATGAACATCATTTTTATGTGTTAACAGATGTTGGGGAAAAGAAATTAATGGAACATTTTGAGGGA
GTAGTTTGGGTAAAAAATTATCCCTGTAAAAGTGTCCCTTTTTATCAAAGGTTCTCAACAGATCATCTATTTGCCTATAA
TGCAGATTTGTTGTTAGGAATAGGCGAAACTGTAGGTTGTGGAGAAAGATGCGAAACATATGAAGAAGTTGTAGAAAATA
TGAATTATTTACAAGTAGATGCTTCTGAATATAGTTGGTATTTAGAAATGAAAAAAACAAGACCTATGAAATCTTCAGGA
TTTGGTTTAGGAGTAGAGCGTTTAATAGCGTGGATATTGAAGTGCGATGATGTTCGAAAAGTTCAATTAATCAGTAGAGA
AAATGGGATTGATTATTTACCATAA

Upstream 100 bases:

>100_bases
AAATTTTAACGCTAACTCCCTATGATGAATCACATTTTTTTAAAATGAAGAAATTTCAACAGTACATTTACCGATAACAA
CATCGTCAATCACAAGTCCT

Downstream 100 bases:

>100_bases
AGGAGGAAGAAAAATGAAAAAGAATATTTTAATTGTAGGTGGAGGTCCAGAGATAACTATATGTTATGATTTAATTAACA
ATAGTACGGAGTATAATTTC

Product: asparagine synthetase A

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MGLGSDSLPVKISLFDVDTYLADSMQFHLEYILRLFPKSGVHYLMPTFRGEAVDHRHLSQFYHSECEIEGDLHYVMNLVD
RYIKFLVSEILRECRDSIFAITKDVSHLESFIDRKIPIITFEEACTILENEIDSLEYNDEHHFYVLTDVGEKKLMEHFEG
VVWVKNYPCKSVPFYQRFSTDHLFAYNADLLLGIGETVGCGERCETYEEVVENMNYLQVDASEYSWYLEMKKTRPMKSSG
FGLGVERLIAWILKCDDVRKVQLISRENGIDYLP

Sequences:

>Translated_274_residues
MGLGSDSLPVKISLFDVDTYLADSMQFHLEYILRLFPKSGVHYLMPTFRGEAVDHRHLSQFYHSECEIEGDLHYVMNLVD
RYIKFLVSEILRECRDSIFAITKDVSHLESFIDRKIPIITFEEACTILENEIDSLEYNDEHHFYVLTDVGEKKLMEHFEG
VVWVKNYPCKSVPFYQRFSTDHLFAYNADLLLGIGETVGCGERCETYEEVVENMNYLQVDASEYSWYLEMKKTRPMKSSG
FGLGVERLIAWILKCDDVRKVQLISRENGIDYLP
>Mature_273_residues
GLGSDSLPVKISLFDVDTYLADSMQFHLEYILRLFPKSGVHYLMPTFRGEAVDHRHLSQFYHSECEIEGDLHYVMNLVDR
YIKFLVSEILRECRDSIFAITKDVSHLESFIDRKIPIITFEEACTILENEIDSLEYNDEHHFYVLTDVGEKKLMEHFEGV
VWVKNYPCKSVPFYQRFSTDHLFAYNADLLLGIGETVGCGERCETYEEVVENMNYLQVDASEYSWYLEMKKTRPMKSSGF
GLGVERLIAWILKCDDVRKVQLISRENGIDYLP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI226491502, Length=264, Percent_Identity=30.6818181818182, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4758762, Length=270, Percent_Identity=25.5555555555556, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1787161, Length=258, Percent_Identity=33.7209302325581, Blast_Score=108, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17556372, Length=256, Percent_Identity=26.953125, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319872, Length=287, Percent_Identity=27.1777003484321, Blast_Score=82, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=270, Percent_Identity=23.3333333333333, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922416, Length=266, Percent_Identity=24.4360902255639, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921528, Length=274, Percent_Identity=25.5474452554745, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 31994; Mature: 31863

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLGSDSLPVKISLFDVDTYLADSMQFHLEYILRLFPKSGVHYLMPTFRGEAVDHRHLSQ
CCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHH
FYHSECEIEGDLHYVMNLVDRYIKFLVSEILRECRDSIFAITKDVSHLESFIDRKIPIIT
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FEEACTILENEIDSLEYNDEHHFYVLTDVGEKKLMEHFEGVVWVKNYPCKSVPFYQRFST
HHHHHHHHHHHHHCCEECCCCCEEEEECCCHHHHHHHHCCEEEEECCCCCCCCHHHHCCC
DHLFAYNADLLLGIGETVGCGERCETYEEVVENMNYLQVDASEYSWYLEMKKTRPMKSSG
CEEEEECCCEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCCCCCC
FGLGVERLIAWILKCDDVRKVQLISRENGIDYLP
CCCCHHHHHHHHHCCCCCHHHHEECCCCCCCCCC
>Mature Secondary Structure 
GLGSDSLPVKISLFDVDTYLADSMQFHLEYILRLFPKSGVHYLMPTFRGEAVDHRHLSQ
CCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHH
FYHSECEIEGDLHYVMNLVDRYIKFLVSEILRECRDSIFAITKDVSHLESFIDRKIPIIT
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FEEACTILENEIDSLEYNDEHHFYVLTDVGEKKLMEHFEGVVWVKNYPCKSVPFYQRFST
HHHHHHHHHHHHHCCEECCCCCEEEEECCCHHHHHHHHCCEEEEECCCCCCCCHHHHCCC
DHLFAYNADLLLGIGETVGCGERCETYEEVVENMNYLQVDASEYSWYLEMKKTRPMKSSG
CEEEEECCCEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCCCCCC
FGLGVERLIAWILKCDDVRKVQLISRENGIDYLP
CCCCHHHHHHHHHCCCCCHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]