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The map label for this gene is tuf [H]

Identifier: 71893875

GI number: 71893875

Start: 676055

End: 677263

Strand: Reverse

Name: tuf [H]

Synonym: MHJ_0524

Alternate gene names: 71893875

Gene position: 677263-676055 (Counterclockwise)

Preceding gene: 71893876

Following gene: 71893873

Centisome position: 75.47

GC content: 40.36

Gene sequence:

>1209_bases
ATGGCAGTTGTTAAAACGACAGGAAAAAAAGACTTTGATCGTTCCAAAGAACATATTAATATTGGAACAATTGGTCATGT
TGACCATGGAAAAACAACACTAACAGCAGCAATTTCAACTGTTTTGGCAAAAAGAGGACTCGCGGAGGCAAAGGATTATG
CCTCAATTGATGCAGCTCCTGAAGAAAAAGCACGAGGAATTACAATTAATACCGCCCATATTGAATATAGCACTGACAAA
CGGCACTATGCCCATGTTGATTGCCCAGGTCATGCCGATTATATAAAAAATATGATCACCGGAGCGGCCCAAATGGATGG
AGCAATTTTAGTGGTTGCAGCAACCGATGGCCCAATGCCTCAAACTCGTGAACACATTTTATTATCAAAACAAGTTGGTG
TCCCAAAAATGGTTGTTTTCCTTAATAAAATTGACTTACTTGAAGGCGAAGAAGAAATGGTTGATTTAGTTGAGGTCGAA
ATTCGCGAACTTCTTTCTTCTTATGATTTTGATGGCGATAATACTCCAATTATTCGGGGATCTGCTCGTGGGGCCCTTGA
AGGAAAACCTGAATGGGAGGCAAAAGTACTTGAATTGATGGACGCAGTTGATTCTTATATTGACTCACCTGTTCGGGAAA
TGGATAAACCCTTTTTGATGGCCGTTGAGGATGTTTTTACAATTACAGGACGTGGAACCGTAGCAACGGGAAAAGTTGAA
AGAGGTCAAGTTAAACTAAACGAAGAAGTGGAAATTGTGGGTTATCGCGAAGAACCTAAAAAGACCGTAATCACCGGAAT
TGAAATGTTTAATAAAAATCTTCAAACTGCGATGGCTGGGGATAATGCCGGTGTTCTCCTACGTGGAGTAGATCGAAAAG
ATATCGAACGTGGTCAGGTGATTGCAAAACCAAAAACAATTATACCGCATACAAAATTTAAAGCTGCAATTTATGCCCTT
AAAAAAGAAGAAGGTGGAAGGCATACACCATTTTTCAAAAATTATAAACCACAATTTTATTTTCGAACTACCGATGTAAC
AGGCGGAATCGAGTTTGAACCAGGTCGCGAGATGGTAATTCCGGGGGATAATGTTGATCTTACCGTTGAATTAATTGCCC
CAATTGCCGTTGAGCAGGGAACCAAATTCTCGATCCGAGAAGGTGGTAGAACCGTGGGTGCCGGAACAGTTACCGAAATT
ATTAAATAA

Upstream 100 bases:

>100_bases
GCAAGGCTATTCAGCGGTGGTTTTTTGCAAGGTTTTATTAAAACAATAATATAATATAATATAATTACGATATAATAAAT
GATAAGCGAAAGGAAAAAAT

Downstream 100 bases:

>100_bases
TTTTTAATATTTAAAAATAATTTAAAATATTCATAAAAAAACTTCAGAATAAACTGAAGTTTTTTATTTTTGAAATAAAT
TTTTTTAGTGTATAATTTTT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MAVVKTTGKKDFDRSKEHINIGTIGHVDHGKTTLTAAISTVLAKRGLAEAKDYASIDAAPEEKARGITINTAHIEYSTDK
RHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHILLSKQVGVPKMVVFLNKIDLLEGEEEMVDLVEVE
IRELLSSYDFDGDNTPIIRGSARGALEGKPEWEAKVLELMDAVDSYIDSPVREMDKPFLMAVEDVFTITGRGTVATGKVE
RGQVKLNEEVEIVGYREEPKKTVITGIEMFNKNLQTAMAGDNAGVLLRGVDRKDIERGQVIAKPKTIIPHTKFKAAIYAL
KKEEGGRHTPFFKNYKPQFYFRTTDVTGGIEFEPGREMVIPGDNVDLTVELIAPIAVEQGTKFSIREGGRTVGAGTVTEI
IK

Sequences:

>Translated_402_residues
MAVVKTTGKKDFDRSKEHINIGTIGHVDHGKTTLTAAISTVLAKRGLAEAKDYASIDAAPEEKARGITINTAHIEYSTDK
RHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHILLSKQVGVPKMVVFLNKIDLLEGEEEMVDLVEVE
IRELLSSYDFDGDNTPIIRGSARGALEGKPEWEAKVLELMDAVDSYIDSPVREMDKPFLMAVEDVFTITGRGTVATGKVE
RGQVKLNEEVEIVGYREEPKKTVITGIEMFNKNLQTAMAGDNAGVLLRGVDRKDIERGQVIAKPKTIIPHTKFKAAIYAL
KKEEGGRHTPFFKNYKPQFYFRTTDVTGGIEFEPGREMVIPGDNVDLTVELIAPIAVEQGTKFSIREGGRTVGAGTVTEI
IK
>Mature_401_residues
AVVKTTGKKDFDRSKEHINIGTIGHVDHGKTTLTAAISTVLAKRGLAEAKDYASIDAAPEEKARGITINTAHIEYSTDKR
HYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHILLSKQVGVPKMVVFLNKIDLLEGEEEMVDLVEVEI
RELLSSYDFDGDNTPIIRGSARGALEGKPEWEAKVLELMDAVDSYIDSPVREMDKPFLMAVEDVFTITGRGTVATGKVER
GQVKLNEEVEIVGYREEPKKTVITGIEMFNKNLQTAMAGDNAGVLLRGVDRKDIERGQVIAKPKTIIPHTKFKAAIYALK
KEEGGRHTPFFKNYKPQFYFRTTDVTGGIEFEPGREMVIPGDNVDLTVELIAPIAVEQGTKFSIREGGRTVGAGTVTEII
K

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=54.6835443037975, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=29.1284403669725, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=29.1284403669725, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI54607086, Length=383, Percent_Identity=31.8537859007833, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI4503475, Length=445, Percent_Identity=28.314606741573, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI4503471, Length=447, Percent_Identity=29.5302013422819, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI194018522, Length=330, Percent_Identity=27.5757575757576, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI194018520, Length=330, Percent_Identity=27.5757575757576, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI194097354, Length=330, Percent_Identity=27.5757575757576, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI46094014, Length=330, Percent_Identity=26.969696969697, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI4503507, Length=271, Percent_Identity=29.8892988929889, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=35.8208955223881, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=68.1933842239186, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=68.1933842239186, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI2367247, Length=337, Percent_Identity=31.4540059347181, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=30.2904564315353, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1789108, Length=329, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788922, Length=141, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=407, Percent_Identity=49.6314496314496, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=44.3298969072165, Blast_Score=317, Evalue=9e-87,
Organism=Caenorhabditis elegans, GI17552884, Length=336, Percent_Identity=34.5238095238095, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=336, Percent_Identity=34.5238095238095, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=443, Percent_Identity=27.0880361173815, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=443, Percent_Identity=27.0880361173815, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=305, Percent_Identity=32.4590163934426, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI32566303, Length=319, Percent_Identity=34.1692789968652, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=326, Percent_Identity=29.1411042944785, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17509919, Length=273, Percent_Identity=28.2051282051282, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=40.8163265306122, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=243, Percent_Identity=26.3374485596708, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=63.727959697733, Blast_Score=516, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6325337, Length=443, Percent_Identity=27.9909706546275, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=443, Percent_Identity=27.9909706546275, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6320377, Length=340, Percent_Identity=30, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322937, Length=332, Percent_Identity=25.3012048192771, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=272, Percent_Identity=29.7794117647059, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=240, Percent_Identity=28.75, Blast_Score=69, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=146, Percent_Identity=32.1917808219178, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281363316, Length=395, Percent_Identity=57.4683544303797, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI17864358, Length=395, Percent_Identity=57.4683544303797, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=48.7113402061856, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=29.8440979955457, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=29.8440979955457, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=29.8440979955457, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=29.8440979955457, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=29.1759465478842, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=29.1759465478842, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=29.5871559633028, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=31.9078947368421, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17137380, Length=327, Percent_Identity=29.0519877675841, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24647048, Length=273, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24647046, Length=273, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 44123; Mature: 43992

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVKTTGKKDFDRSKEHINIGTIGHVDHGKTTLTAAISTVLAKRGLAEAKDYASIDAAP
CEEEECCCCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCC
EEKARGITINTAHIEYSTDKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMP
HHHCCCEEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCC
QTREHILLSKQVGVPKMVVFLNKIDLLEGEEEMVDLVEVEIRELLSSYDFDGDNTPIIRG
CCHHHEEEECCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEC
SARGALEGKPEWEAKVLELMDAVDSYIDSPVREMDKPFLMAVEDVFTITGRGTVATGKVE
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEHEEEECCCEEEECCCC
RGQVKLNEEVEIVGYREEPKKTVITGIEMFNKNLQTAMAGDNAGVLLRGVDRKDIERGQV
CCEEEECCCEEEEEECCCCCHHHHHHHHHHCCCHHHHCCCCCCCEEEECCCHHHHCCCEE
IAKPKTIIPHTKFKAAIYALKKEEGGRHTPFFKNYKPQFYFRTTDVTGGIEFEPGREMVI
EECCCEECCCCHHHHHEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCEEEE
PGDNVDLTVELIAPIAVEQGTKFSIREGGRTVGAGTVTEIIK
CCCCCCEEEEEEECHHHCCCCEEEECCCCEEECCCHHHHHCC
>Mature Secondary Structure 
AVVKTTGKKDFDRSKEHINIGTIGHVDHGKTTLTAAISTVLAKRGLAEAKDYASIDAAP
EEEECCCCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCC
EEKARGITINTAHIEYSTDKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMP
HHHCCCEEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCC
QTREHILLSKQVGVPKMVVFLNKIDLLEGEEEMVDLVEVEIRELLSSYDFDGDNTPIIRG
CCHHHEEEECCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEC
SARGALEGKPEWEAKVLELMDAVDSYIDSPVREMDKPFLMAVEDVFTITGRGTVATGKVE
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEHEEEECCCEEEECCCC
RGQVKLNEEVEIVGYREEPKKTVITGIEMFNKNLQTAMAGDNAGVLLRGVDRKDIERGQV
CCEEEECCCEEEEEECCCCCHHHHHHHHHHCCCHHHHCCCCCCCEEEECCCHHHHCCCEE
IAKPKTIIPHTKFKAAIYALKKEEGGRHTPFFKNYKPQFYFRTTDVTGGIEFEPGREMVI
EECCCEECCCCHHHHHEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCEEEE
PGDNVDLTVELIAPIAVEQGTKFSIREGGRTVGAGTVTEIIK
CCCCCCEEEEEEECHHHCCCCEEEECCCCEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA