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The map label for this gene is glpF [H]

Identifier: 71893707

GI number: 71893707

Start: 431698

End: 432444

Strand: Reverse

Name: glpF [H]

Synonym: MHJ_0354

Alternate gene names: 71893707

Gene position: 432444-431698 (Counterclockwise)

Preceding gene: 71893708

Following gene: 71893705

Centisome position: 48.19

GC content: 32.13

Gene sequence:

>747_bases
ATGAATAATCTCTTTTTTTTATCAGAATTTCTTGGGACTGCGATACTAATTTTACTCGGAAATGGCGTAAATTATAGTGT
AAATGCGACTAAAATGTTTGCAAATCAATCTGGGAAATGAATTGTGATTGCACTAGGTTGAGCTCTTGGGGTACTTTTAG
GAATAATTATTTCAAATGGAATTATCGAAAATGCGCCGGCTCATCTAAATCCGGCAGTTTCAATATTTTTTGGCATTATT
TTTCAAAATAGCGAATTACTTGCGCTGATTCCTTTTCAGTTTTTAGGGGCAATTTTTGCTCAAGTTATTCTAAATTTTAT
CAACTGAAGTCATATAAAGGAAACAAAAGCAGAAATTATCGCTGCCTGCCATCATACTAGCCCTGCTTTTAAAAAATCAT
ATATATCTAATTTTTTCTATGAATTTATCGGAACTACCGTTTTATTAGCAGCAATTTTTTTATTAAATAAAACTTTTTCA
ACAATGAAACCAGTAATTGTTGCACTAATTGTGCTCTCACTTGGACTTTCACTAGGATCTTCGACTGGATATGCAGTTAA
TCCCGCCCGTGATTTTGGCCCAAGATTAGTTTATTTTCTTTTTGCTAATCTAGTTTTAAAAAGAAGAAAGGAATTTGCTG
CCATTAAAAATTGGCAAGAGATTTTTGGATTTTCTTATTCCTTAAATCCGATTATTGCTCCTAGTTTAGCAGGTCTAATT
CTTGGATTAATTATTTATGCACTTTAG

Upstream 100 bases:

>100_bases
TTTGACCAATTTATGGTAAAAAAATTAACCTCCAATTGGGACTTAGCTGTTAGAAAAACGCTAAATTGGAAAAAAGATAT
TAAATAAAAAGGAATCCAAG

Downstream 100 bases:

>100_bases
ATAATTTTATCGACCATAAAACAAAAAAAGACTATAAATTTATAGTCTTTTTTTAAATTTTTAGTGATTTTATTTAGCTT
ATTAAAACCACAAAAAAGAA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MNNLFFLSEFLGTAILILLGNGVNYSVNATKMFANQSGKWIVIALGWALGVLLGIIISNGIIENAPAHLNPAVSIFFGII
FQNSELLALIPFQFLGAIFAQVILNFINWSHIKETKAEIIAACHHTSPAFKKSYISNFFYEFIGTTVLLAAIFLLNKTFS
TMKPVIVALIVLSLGLSLGSSTGYAVNPARDFGPRLVYFLFANLVLKRRKEFAAIKNWQEIFGFSYSLNPIIAPSLAGLI
LGLIIYAL

Sequences:

>Translated_248_residues
MNNLFFLSEFLGTAILILLGNGVNYSVNATKMFANQSGK*IVIALG*ALGVLLGIIISNGIIENAPAHLNPAVSIFFGII
FQNSELLALIPFQFLGAIFAQVILNFIN*SHIKETKAEIIAACHHTSPAFKKSYISNFFYEFIGTTVLLAAIFLLNKTFS
TMKPVIVALIVLSLGLSLGSSTGYAVNPARDFGPRLVYFLFANLVLKRRKEFAAIKNWQEIFGFSYSLNPIIAPSLAGLI
LGLIIYAL
>Mature_248_residues
MNNLFFLSEFLGTAILILLGNGVNYSVNATKMFANQSGK*IVIALG*ALGVLLGIIISNGIIENAPAHLNPAVSIFFGII
FQNSELLALIPFQFLGAIFAQVILNFIN*SHIKETKAEIIAACHHTSPAFKKSYISNFFYEFIGTTVLLAAIFLLNKTFS
TMKPVIVALIVLSLGLSLGSSTGYAVNPARDFGPRLVYFLFANLVLKRRKEFAAIKNWQEIFGFSYSLNPIIAPSLAGLI
LGLIIYAL

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=258, Percent_Identity=31.3953488372093, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI157266307, Length=262, Percent_Identity=29.7709923664122, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI22538420, Length=259, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1790362, Length=211, Percent_Identity=28.9099526066351, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71992966, Length=255, Percent_Identity=26.2745098039216, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=203, Percent_Identity=31.5270935960591, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 26584; Mature: 26584

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLFFLSEFLGTAILILLGNGVNYSVNATKMFANQSGKIVIALGALGVLLGIIISNGII
CCCHHHHHHHHHHHHHHHHCCCCCEECCHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCC
ENAPAHLNPAVSIFFGIIFQNSELLALIPFQFLGAIFAQVILNFINSHIKETKAEIIAAC
CCCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HHTSPAFKKSYISNFFYEFIGTTVLLAAIFLLNKTFSTMKPVIVALIVLSLGLSLGSSTG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
YAVNPARDFGPRLVYFLFANLVLKRRKEFAAIKNWQEIFGFSYSLNPIIAPSLAGLILGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IIYAL
HHHHC
>Mature Secondary Structure
MNNLFFLSEFLGTAILILLGNGVNYSVNATKMFANQSGKIVIALGALGVLLGIIISNGII
CCCHHHHHHHHHHHHHHHHCCCCCEECCHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCC
ENAPAHLNPAVSIFFGIIFQNSELLALIPFQFLGAIFAQVILNFINSHIKETKAEIIAAC
CCCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HHTSPAFKKSYISNFFYEFIGTTVLLAAIFLLNKTFSTMKPVIVALIVLSLGLSLGSSTG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
YAVNPARDFGPRLVYFLFANLVLKRRKEFAAIKNWQEIFGFSYSLNPIIAPSLAGLILGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IIYAL
HHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12949158 [H]