Click here to switch to the map view.

The map label for this gene is glpK [H]

Identifier: 71893708

GI number: 71893708

Start: 432458

End: 433990

Strand: Reverse

Name: glpK [H]

Synonym: MHJ_0355

Alternate gene names: 71893708

Gene position: 433990-432458 (Counterclockwise)

Preceding gene: 71893721

Following gene: 71893707

Centisome position: 48.36

GC content: 34.31

Gene sequence:

>1533_bases
ATGCTAAAAAATACAGCTGAAAAATTTATTATCACCCTCGATTCGGGGACAACTTCATGTCGAAGTTTGATTTTTGACCA
AAAAGGTAGGGTTATTTCGCTTGTCCAAAAAGAATTTAGTCAACATTTTCCCAGATCAGGATGAGTCGAACATGACCCGA
TTGAAATATGGAATACTCAGGTCTATACAATGCAGGCAGCAAAAACAAAAGCAGGGTTAAAATCGACTGATTTTGTTGCC
CTTGGACTAACAAATCAACGGGAAACTGTCGTTTTATGAGATAAAACAAATGGCGAGCCAGTCTATAATGCAATTGTCTG
GCAGGATCGCCGCACGAGTGATTTTTGTGATTATTTAATTGAAAAAGGTTATCAAAATTATATAAAAGAAGTAACAGGTC
TAATTATTAATCCTTATTTTAGCGGGACAAAAATTCGCTGAATTTTAAAAAATGTTAAAAAAGCAAAGGAAGTTTTAGCT
GCTGGTAATCTTTTAGCAGGAACAATTGATAGCTGACTTCTTTGAAAATTAACTAACGGAAAAGTACATGCAACTGATAT
AACAAACGCTTCAAGAACGATGTTATTTGATATAAAAAAACGAAAATGAGACCAAAAAATTCTTGATCTTTTAGAAATTC
CTTACAGTATTCTCCCAAAAGTTTTAGCATCAGCAGATGATTTTGGATTTGTTTCTCCTTTTCATTGATCAATTAATGCA
AAAGCAAAAGTACCAATTTATGCACTAATCGGTGATCAACAATCGGCCTTATTTGGTCAACTTGCAACTGAATTTGGAAT
GGTTAAAAATACCTATGGAACCGGATGTTTTATGCTAGTTAATACTGGTGAAAAACTAATAAAATCAAAGAATAATTTAC
TTACAACAGTGGCCTGACAAATCGGAAATAATCCAATCCAGTACGCACTTGAAGGATCAGTTTTTATTGCCGGAGCCGCA
ATCCAGTGGCTCAGAGATGGACTTTCTTTGATTGAAAACTCTTCAGATTGCGATTCTTTAGTCTCAAAAGCTAAAAAAAA
TGATTATCATATTATTCTTGTTCCATCTTTTACCGGACTTGGGGCACCTTATTGGGACTCCTATTCGCGTGGGGCAATTT
TTGGACTTGAAAGGGGAACAAAAAAAGAACATCTTATCAAAGCAAGTCTTGAATCAATTGCATTTCAGGCAAATGATCTA
ATTAAGGCGATCGAATCAGATCTAGGAACAAAAATCAGCTTGATGAAAGTTGATGGTGGAGTTTCTAAAAGTGATTATCT
AATGCAATTTCAGGCATCAATTTCGCAGGTTGAAATCCAAAGGCCAAAAAATATTGAAACAACAGCAATGGGAGCAGCTT
TTCTTGCAGGTTTATATGCCAAATTTTGGAATTCACTAGCTGAATTAAAGCAAATTTTAGTTCCTGAAAAAGTTTTTGTC
CCTGAATTTGACCAATTTATGGTAAAAAAATTAACCTCCAATTGGGACTTAGCTGTTAGAAAAACGCTAAATTGGAAAAA
AGATATTAAATAA

Upstream 100 bases:

>100_bases
AAAAATAATTAAAAAATTAACAAAATTGTTAATTTCACAGGGCTACTTTGGATTAAAATGTTATAATTCAAATATATAAA
AATTGAAAGGATTTAAAAAA

Downstream 100 bases:

>100_bases
AAAGGAATCCAAGATGAATAATCTCTTTTTTTTATCAGAATTTCTTGGGACTGCGATACTAATTTTACTCGGAAATGGCG
TAAATTATAGTGTAAATGCG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MLKNTAEKFIITLDSGTTSCRSLIFDQKGRVISLVQKEFSQHFPRSGWVEHDPIEIWNTQVYTMQAAKTKAGLKSTDFVA
LGLTNQRETVVLWDKTNGEPVYNAIVWQDRRTSDFCDYLIEKGYQNYIKEVTGLIINPYFSGTKIRWILKNVKKAKEVLA
AGNLLAGTIDSWLLWKLTNGKVHATDITNASRTMLFDIKKRKWDQKILDLLEIPYSILPKVLASADDFGFVSPFHWSINA
KAKVPIYALIGDQQSALFGQLATEFGMVKNTYGTGCFMLVNTGEKLIKSKNNLLTTVAWQIGNNPIQYALEGSVFIAGAA
IQWLRDGLSLIENSSDCDSLVSKAKKNDYHIILVPSFTGLGAPYWDSYSRGAIFGLERGTKKEHLIKASLESIAFQANDL
IKAIESDLGTKISLMKVDGGVSKSDYLMQFQASISQVEIQRPKNIETTAMGAAFLAGLYAKFWNSLAELKQILVPEKVFV
PEFDQFMVKKLTSNWDLAVRKTLNWKKDIK

Sequences:

>Translated_510_residues
MLKNTAEKFIITLDSGTTSCRSLIFDQKGRVISLVQKEFSQHFPRSG*VEHDPIEIWNTQVYTMQAAKTKAGLKSTDFVA
LGLTNQRETVVL*DKTNGEPVYNAIVWQDRRTSDFCDYLIEKGYQNYIKEVTGLIINPYFSGTKIR*ILKNVKKAKEVLA
AGNLLAGTIDS*LL*KLTNGKVHATDITNASRTMLFDIKKRK*DQKILDLLEIPYSILPKVLASADDFGFVSPFH*SINA
KAKVPIYALIGDQQSALFGQLATEFGMVKNTYGTGCFMLVNTGEKLIKSKNNLLTTVA*QIGNNPIQYALEGSVFIAGAA
IQWLRDGLSLIENSSDCDSLVSKAKKNDYHIILVPSFTGLGAPYWDSYSRGAIFGLERGTKKEHLIKASLESIAFQANDL
IKAIESDLGTKISLMKVDGGVSKSDYLMQFQASISQVEIQRPKNIETTAMGAAFLAGLYAKFWNSLAELKQILVPEKVFV
PEFDQFMVKKLTSNWDLAVRKTLNWKKDIK
>Mature_510_residues
MLKNTAEKFIITLDSGTTSCRSLIFDQKGRVISLVQKEFSQHFPRSG*VEHDPIEIWNTQVYTMQAAKTKAGLKSTDFVA
LGLTNQRETVVL*DKTNGEPVYNAIVWQDRRTSDFCDYLIEKGYQNYIKEVTGLIINPYFSGTKIR*ILKNVKKAKEVLA
AGNLLAGTIDS*LL*KLTNGKVHATDITNASRTMLFDIKKRK*DQKILDLLEIPYSILPKVLASADDFGFVSPFH*SINA
KAKVPIYALIGDQQSALFGQLATEFGMVKNTYGTGCFMLVNTGEKLIKSKNNLLTTVA*QIGNNPIQYALEGSVFIAGAA
IQWLRDGLSLIENSSDCDSLVSKAKKNDYHIILVPSFTGLGAPYWDSYSRGAIFGLERGTKKEHLIKASLESIAFQANDL
IKAIESDLGTKISLMKVDGGVSKSDYLMQFQASISQVEIQRPKNIETTAMGAAFLAGLYAKFWNSLAELKQILVPEKVFV
PEFDQFMVKKLTSNWDLAVRKTLNWKKDIK

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=41.501976284585, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI189409120, Length=504, Percent_Identity=41.0714285714286, Blast_Score=338, Evalue=6e-93,
Organism=Homo sapiens, GI4504007, Length=504, Percent_Identity=41.0714285714286, Blast_Score=338, Evalue=6e-93,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=40.6374501992032, Blast_Score=333, Evalue=3e-91,
Organism=Homo sapiens, GI88196792, Length=512, Percent_Identity=32.2265625, Blast_Score=244, Evalue=2e-64,
Organism=Escherichia coli, GI1790361, Length=503, Percent_Identity=47.9125248508946, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1789987, Length=476, Percent_Identity=22.6890756302521, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535599, Length=510, Percent_Identity=38.4313725490196, Blast_Score=310, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI71986140, Length=527, Percent_Identity=23.3396584440228, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71986147, Length=300, Percent_Identity=25.6666666666667, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321755, Length=527, Percent_Identity=32.6375711574953, Blast_Score=240, Evalue=3e-64,
Organism=Drosophila melanogaster, GI17864214, Length=513, Percent_Identity=37.2319688109162, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI24654573, Length=446, Percent_Identity=38.1165919282511, Blast_Score=298, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24655555, Length=485, Percent_Identity=34.639175257732, Blast_Score=278, Evalue=9e-75,
Organism=Drosophila melanogaster, GI24655560, Length=485, Percent_Identity=34.639175257732, Blast_Score=278, Evalue=9e-75,
Organism=Drosophila melanogaster, GI24655570, Length=485, Percent_Identity=34.639175257732, Blast_Score=278, Evalue=9e-75,
Organism=Drosophila melanogaster, GI24655574, Length=485, Percent_Identity=34.639175257732, Blast_Score=278, Evalue=9e-75,
Organism=Drosophila melanogaster, GI24655565, Length=485, Percent_Identity=34.639175257732, Blast_Score=278, Evalue=9e-75,
Organism=Drosophila melanogaster, GI19922058, Length=532, Percent_Identity=30.6390977443609, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24652871, Length=501, Percent_Identity=30.7385229540918, Blast_Score=218, Evalue=9e-57,
Organism=Drosophila melanogaster, GI21357867, Length=526, Percent_Identity=28.1368821292776, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24656367, Length=512, Percent_Identity=28.515625, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24656372, Length=370, Percent_Identity=29.7297297297297, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45552909, Length=280, Percent_Identity=29.2857142857143, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55659; Mature: 55659

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNTAEKFIITLDSGTTSCRSLIFDQKGRVISLVQKEFSQHFPRSGVEHDPIEIWNTQV
CCCCCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCEE
YTMQAAKTKAGLKSTDFVALGLTNQRETVVLDKTNGEPVYNAIVWQDRRTSDFCDYLIEK
EEEEHHHHHCCCCCCCEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHH
GYQNYIKEVTGLIINPYFSGTKIRILKNVKKAKEVLAAGNLLAGTIDSLLKLTNGKVHAT
HHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEE
DITNASRTMLFDIKKRKDQKILDLLEIPYSILPKVLASADDFGFVSPFHSINAKAKVPIY
ECCCCCCEEEEEHHHHCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCEE
ALIGDQQSALFGQLATEFGMVKNTYGTGCFMLVNTGEKLIKSKNNLLTTVAQIGNNPIQY
EEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHCCCHHHHHHHHHCCCCEEE
ALEGSVFIAGAAIQWLRDGLSLIENSSDCDSLVSKAKKNDYHIILVPSFTGLGAPYWDSY
EECCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCC
SRGAIFGLERGTKKEHLIKASLESIAFQANDLIKAIESDLGTKISLMKVDGGVSKSDYLM
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHH
QFQASISQVEIQRPKNIETTAMGAAFLAGLYAKFWNSLAELKQILVPEKVFVPEFDQFMV
HHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
KKLTSNWDLAVRKTLNWKKDIK
HHHCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MLKNTAEKFIITLDSGTTSCRSLIFDQKGRVISLVQKEFSQHFPRSGVEHDPIEIWNTQV
CCCCCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCEE
YTMQAAKTKAGLKSTDFVALGLTNQRETVVLDKTNGEPVYNAIVWQDRRTSDFCDYLIEK
EEEEHHHHHCCCCCCCEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHH
GYQNYIKEVTGLIINPYFSGTKIRILKNVKKAKEVLAAGNLLAGTIDSLLKLTNGKVHAT
HHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEE
DITNASRTMLFDIKKRKDQKILDLLEIPYSILPKVLASADDFGFVSPFHSINAKAKVPIY
ECCCCCCEEEEEHHHHCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCEE
ALIGDQQSALFGQLATEFGMVKNTYGTGCFMLVNTGEKLIKSKNNLLTTVAQIGNNPIQY
EEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHCCCHHHHHHHHHCCCCEEE
ALEGSVFIAGAAIQWLRDGLSLIENSSDCDSLVSKAKKNDYHIILVPSFTGLGAPYWDSY
EECCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCC
SRGAIFGLERGTKKEHLIKASLESIAFQANDLIKAIESDLGTKISLMKVDGGVSKSDYLM
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHH
QFQASISQVEIQRPKNIETTAMGAAFLAGLYAKFWNSLAELKQILVPEKVFVPEFDQFMV
HHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
KKLTSNWDLAVRKTLNWKKDIK
HHHCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11353084 [H]