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The map label for this gene is folE

Identifier: 71892245

GI number: 71892245

Start: 581657

End: 582322

Strand: Reverse

Name: folE

Synonym: BPEN_487

Alternate gene names: 71892245

Gene position: 582322-581657 (Counterclockwise)

Preceding gene: 71892247

Following gene: 71892243

Centisome position: 73.56

GC content: 30.48

Gene sequence:

>666_bases
ATGTCTATATTAACACAAGAAGCGCTATTAGTACGTGATGCTTTATCAGTTCGAGGATTAGAAAATCCATTAATTGAGTT
GAATATTAATCATAAAATACGTAAACGTCGTATTGAAAACCATATGAGAGCTATTGTGCATCTTCTTAATCTCGATTTGG
AACATGATAGTTTGTTAAATACTCCTAAACGTATAGCTAAGATGTATATAGAAGAAATTTTCTCAGGATTGGATTATTCA
AATTTTCCTAAAATTGCAATTATTCAAAATACAATGCAAATAAATGAGATGATTACAGTGAGAGGTATTAATATCACCAG
CACTTGTGAACATCATTTCATTGTTTTTAATGGTAAAGTGACTATTTCTTATATTCCAGAAAAAAATGTGATTGGTTTGT
CAAAGATAAATAGAATAGTTCAATTTTTTTCTAAAAGACCGCAGTTACAGGAACGATTAACAAAACAAATTTTTTTAGCG
TTGCAAACCTTACTTAATACAGATAATGTGGCAATATTTATTGATGCAGTACATTATTGTGTTAAAGCTAGGGGTATTCA
TGATGTTAGCAGCACTACAACTACTACTGCGCTAGGGGGGTTATTTGAATCTAATACGAATACTAGAGAAGAATTTCTGC
ACGCAATTATGTATTGTAACCATTGA

Upstream 100 bases:

>100_bases
TCAATGTATACTTGATTTAATTAAAATGTTTCGAAGGTGATTAGTACGATATAATATTACGCGAAAAGTTTTTCTTGAAT
GTATTTTTAGAGAAAGTTAT

Downstream 100 bases:

>100_bases
TTTATATTTTTTTATTTAAAATTTAATAAGGTTATTATATATCATATAATATGAATTTTTAAAGACATAGCAATCTGATT
TATAATATTTGTTTAAGTAT

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MSILTQEALLVRDALSVRGLENPLIELNINHKIRKRRIENHMRAIVHLLNLDLEHDSLLNTPKRIAKMYIEEIFSGLDYS
NFPKIAIIQNTMQINEMITVRGINITSTCEHHFIVFNGKVTISYIPEKNVIGLSKINRIVQFFSKRPQLQERLTKQIFLA
LQTLLNTDNVAIFIDAVHYCVKARGIHDVSSTTTTTALGGLFESNTNTREEFLHAIMYCNH

Sequences:

>Translated_221_residues
MSILTQEALLVRDALSVRGLENPLIELNINHKIRKRRIENHMRAIVHLLNLDLEHDSLLNTPKRIAKMYIEEIFSGLDYS
NFPKIAIIQNTMQINEMITVRGINITSTCEHHFIVFNGKVTISYIPEKNVIGLSKINRIVQFFSKRPQLQERLTKQIFLA
LQTLLNTDNVAIFIDAVHYCVKARGIHDVSSTTTTTALGGLFESNTNTREEFLHAIMYCNH
>Mature_220_residues
SILTQEALLVRDALSVRGLENPLIELNINHKIRKRRIENHMRAIVHLLNLDLEHDSLLNTPKRIAKMYIEEIFSGLDYSN
FPKIAIIQNTMQINEMITVRGINITSTCEHHFIVFNGKVTISYIPEKNVIGLSKINRIVQFFSKRPQLQERLTKQIFLAL
QTLLNTDNVAIFIDAVHYCVKARGIHDVSSTTTTTALGGLFESNTNTREEFLHAIMYCNH

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=177, Percent_Identity=38.9830508474576, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI4503949, Length=177, Percent_Identity=38.9830508474576, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI66932972, Length=140, Percent_Identity=38.5714285714286, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI66932970, Length=140, Percent_Identity=38.5714285714286, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1788476, Length=222, Percent_Identity=63.0630630630631, Blast_Score=284, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17560486, Length=226, Percent_Identity=37.1681415929204, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6321706, Length=182, Percent_Identity=41.2087912087912, Blast_Score=135, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24656796, Length=198, Percent_Identity=35.3535353535354, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24656782, Length=198, Percent_Identity=35.3535353535354, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24656791, Length=206, Percent_Identity=33.495145631068, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_BLOPB (Q492J7)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277979.1
- HSSP:   Q5SH52
- ProteinModelPortal:   Q492J7
- SMR:   Q492J7
- STRING:   Q492J7
- GeneID:   3562994
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_487
- eggNOG:   COG0302
- HOGENOM:   HBG370191
- OMA:   RSRQEFF
- ProtClustDB:   PRK09347
- BioCyc:   CBLO291272:BPEN_487-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 25355; Mature: 25224

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILTQEALLVRDALSVRGLENPLIELNINHKIRKRRIENHMRAIVHLLNLDLEHDSLLN
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHC
TPKRIAKMYIEEIFSGLDYSNFPKIAIIQNTMQINEMITVRGINITSTCEHHFIVFNGKV
CHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHEEEEECCCEECCCCCEEEEEECEE
TISYIPEKNVIGLSKINRIVQFFSKRPQLQERLTKQIFLALQTLLNTDNVAIFIDAVHYC
EEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
VKARGIHDVSSTTTTTALGGLFESNTNTREEFLHAIMYCNH
HHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SILTQEALLVRDALSVRGLENPLIELNINHKIRKRRIENHMRAIVHLLNLDLEHDSLLN
CCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHC
TPKRIAKMYIEEIFSGLDYSNFPKIAIIQNTMQINEMITVRGINITSTCEHHFIVFNGKV
CHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHEEEEECCCEECCCCCEEEEEECEE
TISYIPEKNVIGLSKINRIVQFFSKRPQLQERLTKQIFLALQTLLNTDNVAIFIDAVHYC
EEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
VKARGIHDVSSTTTTTALGGLFESNTNTREEFLHAIMYCNH
HHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA