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The map label for this gene is ddl

Identifier: 71892247

GI number: 71892247

Start: 583897

End: 585003

Strand: Reverse

Name: ddl

Synonym: BPEN_490

Alternate gene names: 71892247

Gene position: 585003-583897 (Counterclockwise)

Preceding gene: 71892248

Following gene: 71892245

Centisome position: 73.9

GC content: 30.8

Gene sequence:

>1107_bases
ATGTCTAAATTATGCATTGGTATTATTTGCGGGGGTCGTTCTTTAGAACACGAGATTTCATTGAAATCAGCTATGTGTAT
TGCACAGTTTATTGATAAATTTCGTTTCGAGGCCATGATTTTATGGATAGATAAAAAAGGATGTTGGCATTTGAAAGATA
TCGATTTTAATGATTTATCGTATCATAAAGATGAAAAAATTTCCATTTTGTTACAAAACTGTCCTTATCCGTTTAAATTT
CATACTAAAAATATAAATATTTTATTAAAATTTGATGTTATTTTCCCTGTAGTTCATGGAACACTAGGAGAAGATGGTGC
TTTGCAAGGTTTATTGTGTATGATGAATTTGCCATTTGTTGGATCTAATGTTTTGAGTTCATCTGTAGGGATGGATAAAG
ACGTGTCTAAATGTTTGTTGCGTGATGCGGGTTTATCCGTAGTACCATTTAGAACTGTTTTGGCTCAGGATCAACATAAT
ATAGATTTTGATTATTTTGTTTCTATCTTTGGACTACCTTTGTTTGTGAAACCATCAAATCAAGGGTCATCGATAGGAGT
ATCGAAAGTTACTAAATGTAAAGATTTTAACTTAGCTTTAATCAAAGCTTTCTCTTTTAGTAATAAAATATTAATAGAAC
CAGCTATTATTGGAAGAGAGTTGGAATGCGCAGTATTGGGTAACGATAATCCGAAAATTAGTGTGTGTGGTGAAGTTATA
TTATCAGATAATAATTTTTATACTTATCATGATAAATATGTGAAACGTAAAGCACAGATTATGATACCTGCGTTAATTAA
TGATGCAATAAGTGATGATATTCGTCATATTGCTTTACGTGCATTTCAAGTGTTAAATTGTTCTGGAATGGCGCGGGTAG
ATGTTTTTTTAAATTTGGATAATAAAATTTTTATTAATGAAGTTAATACGTTACCTGGATTTACTTTCGATAGTATGTAT
CCTAAATTGTGGGAGGTAAGTGGATTAAATTTGCAAGAGCTAATTACGAAATTGGTAGAATTAGCGCTAGATCTACATGA
TAAAAAAAATTGTTTTTATCATGTAGATGGTGCTGTATTGAAATATTTTCGGCATGAGAGGATTTGA

Upstream 100 bases:

>100_bases
TACGCATTTTAAGAATAGGTCGACATTAAATACTAAAACAGGCGAATTAAAATATTAATTCGCCTGTTTTAGTATTTTTT
AATGTATCAAGGAATGGAAT

Downstream 100 bases:

>100_bases
ACCTCTGACCCCCGACACCCCATGACGGTGCGCTACCAAACTACGCTACATGCCGTTTTAGGTTTATTATAAAAAATTTG
TAAAAAATATTATTTTTTAT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSKLCIGIICGGRSLEHEISLKSAMCIAQFIDKFRFEAMILWIDKKGCWHLKDIDFNDLSYHKDEKISILLQNCPYPFKF
HTKNINILLKFDVIFPVVHGTLGEDGALQGLLCMMNLPFVGSNVLSSSVGMDKDVSKCLLRDAGLSVVPFRTVLAQDQHN
IDFDYFVSIFGLPLFVKPSNQGSSIGVSKVTKCKDFNLALIKAFSFSNKILIEPAIIGRELECAVLGNDNPKISVCGEVI
LSDNNFYTYHDKYVKRKAQIMIPALINDAISDDIRHIALRAFQVLNCSGMARVDVFLNLDNKIFINEVNTLPGFTFDSMY
PKLWEVSGLNLQELITKLVELALDLHDKKNCFYHVDGAVLKYFRHERI

Sequences:

>Translated_368_residues
MSKLCIGIICGGRSLEHEISLKSAMCIAQFIDKFRFEAMILWIDKKGCWHLKDIDFNDLSYHKDEKISILLQNCPYPFKF
HTKNINILLKFDVIFPVVHGTLGEDGALQGLLCMMNLPFVGSNVLSSSVGMDKDVSKCLLRDAGLSVVPFRTVLAQDQHN
IDFDYFVSIFGLPLFVKPSNQGSSIGVSKVTKCKDFNLALIKAFSFSNKILIEPAIIGRELECAVLGNDNPKISVCGEVI
LSDNNFYTYHDKYVKRKAQIMIPALINDAISDDIRHIALRAFQVLNCSGMARVDVFLNLDNKIFINEVNTLPGFTFDSMY
PKLWEVSGLNLQELITKLVELALDLHDKKNCFYHVDGAVLKYFRHERI
>Mature_367_residues
SKLCIGIICGGRSLEHEISLKSAMCIAQFIDKFRFEAMILWIDKKGCWHLKDIDFNDLSYHKDEKISILLQNCPYPFKFH
TKNINILLKFDVIFPVVHGTLGEDGALQGLLCMMNLPFVGSNVLSSSVGMDKDVSKCLLRDAGLSVVPFRTVLAQDQHNI
DFDYFVSIFGLPLFVKPSNQGSSIGVSKVTKCKDFNLALIKAFSFSNKILIEPAIIGRELECAVLGNDNPKISVCGEVIL
SDNNFYTYHDKYVKRKAQIMIPALINDAISDDIRHIALRAFQVLNCSGMARVDVFLNLDNKIFINEVNTLPGFTFDSMYP
KLWEVSGLNLQELITKLVELALDLHDKKNCFYHVDGAVLKYFRHERI

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=361, Percent_Identity=51.5235457063712, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1786280, Length=341, Percent_Identity=31.0850439882698, Blast_Score=167, Evalue=9e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_BLOPB (Q492J5)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277981.1
- ProteinModelPortal:   Q492J5
- SMR:   Q492J5
- STRING:   Q492J5
- GeneID:   3562997
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_490
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   CBLO291272:BPEN_490-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 41585; Mature: 41454

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLCIGIICGGRSLEHEISLKSAMCIAQFIDKFRFEAMILWIDKKGCWHLKDIDFNDLS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCEEECCCCCCCCC
YHKDEKISILLQNCPYPFKFHTKNINILLKFDVIFPVVHGTLGEDGALQGLLCMMNLPFV
CCCCCEEEEEEECCCCCEEEEECCEEEEEEEEEEHHHHHCCCCCCCHHHHHHHHHCCCCC
GSNVLSSSVGMDKDVSKCLLRDAGLSVVPFRTVLAQDQHNIDFDYFVSIFGLPLFVKPSN
CCHHHHHCCCCCHHHHHHHHHHCCCEEEEHHHHHHCCCCCCCHHHHHHHHCCEEEEECCC
QGSSIGVSKVTKCKDFNLALIKAFSFSNKILIEPAIIGRELECAVLGNDNPKISVCGEVI
CCCCCCHHHHCCCCCCCEEEEEEECCCCEEEEEHHHCCCEEEEEEECCCCCEEEEEEEEE
LSDNNFYTYHDKYVKRKAQIMIPALINDAISDDIRHIALRAFQVLNCSGMARVDVFLNLD
EECCCEEEECHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECC
NKIFINEVNTLPGFTFDSMYPKLWEVSGLNLQELITKLVELALDLHDKKNCFYHVDGAVL
CEEEEEECCCCCCCCHHHCCCHHEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEECHHHH
KYFRHERI
HHHHHCCC
>Mature Secondary Structure 
SKLCIGIICGGRSLEHEISLKSAMCIAQFIDKFRFEAMILWIDKKGCWHLKDIDFNDLS
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCEEECCCCCCCCC
YHKDEKISILLQNCPYPFKFHTKNINILLKFDVIFPVVHGTLGEDGALQGLLCMMNLPFV
CCCCCEEEEEEECCCCCEEEEECCEEEEEEEEEEHHHHHCCCCCCCHHHHHHHHHCCCCC
GSNVLSSSVGMDKDVSKCLLRDAGLSVVPFRTVLAQDQHNIDFDYFVSIFGLPLFVKPSN
CCHHHHHCCCCCHHHHHHHHHHCCCEEEEHHHHHHCCCCCCCHHHHHHHHCCEEEEECCC
QGSSIGVSKVTKCKDFNLALIKAFSFSNKILIEPAIIGRELECAVLGNDNPKISVCGEVI
CCCCCCHHHHCCCCCCCEEEEEEECCCCEEEEEHHHCCCEEEEEEECCCCCEEEEEEEEE
LSDNNFYTYHDKYVKRKAQIMIPALINDAISDDIRHIALRAFQVLNCSGMARVDVFLNLD
EECCCEEEECHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECC
NKIFINEVNTLPGFTFDSMYPKLWEVSGLNLQELITKLVELALDLHDKKNCFYHVDGAVL
CEEEEEECCCCCCCCHHHCCCHHEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEECHHHH
KYFRHERI
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA