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The map label for this gene is ispE

Identifier: 71892125

GI number: 71892125

Start: 421786

End: 422673

Strand: Reverse

Name: ispE

Synonym: BPEN_357

Alternate gene names: 71892125

Gene position: 422673-421786 (Counterclockwise)

Preceding gene: 71892126

Following gene: 71892124

Centisome position: 53.39

GC content: 33.0

Gene sequence:

>888_bases
ATGAATTATCAATGGCCCTCTCCAGGAAAACTTAATTTATTTTTATACGTAACTGGATGTCGTGCAGACGGTTACCATTA
CTTACAGACATTGTTTCAGTTGATTGAGTATGGCGATACGATCAAAATATTTGTAACAAATAATGGTAGAATTAGATTAT
TTACTATTATGAATGATTTGGTATGTCGTGATAATTTAATTATACGAGCGGCAAAATTATTGCAATATTATTGTTGGCCA
AATAAAAAGCCGGTATTCGGTGCAGATATTTTTCTTGATAAAATATTGCCTATCGGATCTGGTTTAGGAGGCGCTTCTTC
TAATGCGGCAACTGTATTGATGGTACTTAATCAACAATGGCGATGTTATTTGAACAAACACGTTTTAATGCATTTAGGTT
TGATGCTAGGAGCTGATGTGCCATTTTTTCTGTATGGACGTTCAGCATTTGCTGAAGGTATTGGAAATATATTAATACCG
GTTTTTGTTCCTAAAAAATGGTACCTCATTCTCATTCCACCGGTTCGAATTAGTACTTTCTGGGGTTTTCAAATGTATGA
ATTAGAGAATCATTGTTATTCTCCATTTCGTTCGATGAGAGAGTTATTGTCTGTTTCATTTCATAATGATTTCGAAGAAA
TTATAAAAAAAATATCTCCTGAAATAAAAATTTTTTTTGCGTGTTTGTCGCAATTCGCATTAGCACGACTAACAGGAACA
GGGTCTTGTATTTTTGCTGAATTTAAGACTGAACAACTTGCTTATCAAGTTCAAAGTTACTTGCCATCATGGGTCAGCAG
TATTATAACACGCGGAATAAATCTATCTCCATTGCATCAAAAATTATTAAAATGTACCGCATATAGTAGTATAATTTTTT
TATCTTAA

Upstream 100 bases:

>100_bases
GTCATTACTATACTAATAGTATACCAATTTTGCCTAAAGTCTTGGATGCGCATTATGATCAACATCATATTAAGCTAATA
ATTAATAATTGGAATGTACG

Downstream 100 bases:

>100_bases
TAAATGTCTTACTATCATCGTCATTATTAATTATATAAATACTTAATAGTATTAGCAAATATAAAATTAAGAGATGCTTC
TAAATATGAAACTGTTTACT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MNYQWPSPGKLNLFLYVTGCRADGYHYLQTLFQLIEYGDTIKIFVTNNGRIRLFTIMNDLVCRDNLIIRAAKLLQYYCWP
NKKPVFGADIFLDKILPIGSGLGGASSNAATVLMVLNQQWRCYLNKHVLMHLGLMLGADVPFFLYGRSAFAEGIGNILIP
VFVPKKWYLILIPPVRISTFWGFQMYELENHCYSPFRSMRELLSVSFHNDFEEIIKKISPEIKIFFACLSQFALARLTGT
GSCIFAEFKTEQLAYQVQSYLPSWVSSIITRGINLSPLHQKLLKCTAYSSIIFLS

Sequences:

>Translated_295_residues
MNYQWPSPGKLNLFLYVTGCRADGYHYLQTLFQLIEYGDTIKIFVTNNGRIRLFTIMNDLVCRDNLIIRAAKLLQYYCWP
NKKPVFGADIFLDKILPIGSGLGGASSNAATVLMVLNQQWRCYLNKHVLMHLGLMLGADVPFFLYGRSAFAEGIGNILIP
VFVPKKWYLILIPPVRISTFWGFQMYELENHCYSPFRSMRELLSVSFHNDFEEIIKKISPEIKIFFACLSQFALARLTGT
GSCIFAEFKTEQLAYQVQSYLPSWVSSIITRGINLSPLHQKLLKCTAYSSIIFLS
>Mature_295_residues
MNYQWPSPGKLNLFLYVTGCRADGYHYLQTLFQLIEYGDTIKIFVTNNGRIRLFTIMNDLVCRDNLIIRAAKLLQYYCWP
NKKPVFGADIFLDKILPIGSGLGGASSNAATVLMVLNQQWRCYLNKHVLMHLGLMLGADVPFFLYGRSAFAEGIGNILIP
VFVPKKWYLILIPPVRISTFWGFQMYELENHCYSPFRSMRELLSVSFHNDFEEIIKKISPEIKIFFACLSQFALARLTGT
GSCIFAEFKTEQLAYQVQSYLPSWVSSIITRGINLSPLHQKLLKCTAYSSIIFLS

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=285, Percent_Identity=51.2280701754386, Blast_Score=268, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_BLOPB (Q492W0)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277857.1
- ProteinModelPortal:   Q492W0
- SMR:   Q492W0
- STRING:   Q492W0
- GeneID:   3562814
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_357
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   WIEEAPC
- BioCyc:   CBLO291272:BPEN_357-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 33750; Mature: 33750

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYQWPSPGKLNLFLYVTGCRADGYHYLQTLFQLIEYGDTIKIFVTNNGRIRLFTIMNDL
CCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEHHHH
VCRDNLIIRAAKLLQYYCWPNKKPVFGADIFLDKILPIGSGLGGASSNAATVLMVLNQQW
HHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHH
RCYLNKHVLMHLGLMLGADVPFFLYGRSAFAEGIGNILIPVFVPKKWYLILIPPVRISTF
HHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHCCCCEEEEEEECCCEEEEEECCEEEEHC
WGFQMYELENHCYSPFRSMRELLSVSFHNDFEEIIKKISPEIKIFFACLSQFALARLTGT
CCEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
GSCIFAEFKTEQLAYQVQSYLPSWVSSIITRGINLSPLHQKLLKCTAYSSIIFLS
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEEC
>Mature Secondary Structure
MNYQWPSPGKLNLFLYVTGCRADGYHYLQTLFQLIEYGDTIKIFVTNNGRIRLFTIMNDL
CCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEHHHH
VCRDNLIIRAAKLLQYYCWPNKKPVFGADIFLDKILPIGSGLGGASSNAATVLMVLNQQW
HHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHH
RCYLNKHVLMHLGLMLGADVPFFLYGRSAFAEGIGNILIPVFVPKKWYLILIPPVRISTF
HHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHCCCCEEEEEEECCCEEEEEECCEEEEHC
WGFQMYELENHCYSPFRSMRELLSVSFHNDFEEIIKKISPEIKIFFACLSQFALARLTGT
CCEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
GSCIFAEFKTEQLAYQVQSYLPSWVSSIITRGINLSPLHQKLLKCTAYSSIIFLS
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA