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The map label for this gene is proS

Identifier: 71892071

GI number: 71892071

Start: 346510

End: 348255

Strand: Reverse

Name: proS

Synonym: BPEN_297

Alternate gene names: 71892071

Gene position: 348255-346510 (Counterclockwise)

Preceding gene: 71892073

Following gene: 71892052

Centisome position: 43.99

GC content: 31.04

Gene sequence:

>1746_bases
ATGCGTACCAGTCAATATTTATTAGCTACTCTTAAAGAGGCGCCTAAGAATTGCAAAGCAATTAGCCATCAACTAATGTT
ACGAGCTGGCTTAATTCGTCAAGTATCATCTGGTCTTTATACTTGGTTACCCACTGGATTACGTGTATTACGGAAAATAG
AAAATATTATTCGAGAAGAAATGAGTAAAATTGGTGCTATTGAAATAGCTATGCCGATAGTTCAACCTGCGAGATTATGG
AAAAAAAGTGGTCGTTGGACAGGATATGGAACAGAATTATTGCGTTTTAAAAATCGCAATAATCAGGAATTTGTATTAGG
ACCAACTCATGAAGAAATGGTGTCAGAAATTATTTGTAAAGAAACAATGCTTTATAAACAGTTTCCATTAATTGTGTATC
AAATTCATACTAAATACCGTGATGAAGCCCGTCCTCGATCTGGAGTAATACGCGCTAGAGAATTCATTATGAAAGATGGA
TATTCTTTTCATATCAATCAGAAATCTCTTCAGAATACATATAATAACATGTACCAAACATATCACACTATTTTTAATCG
TATTGGACTAAATTTTTGTGTTGTTCAAGCTGAACCTGGTAAAATCGGGGGGGCATTGTCTCATGAGTTTCAAGCATATT
CTGAAAATGGCGAAGATAGTATCGCGGTTCCTATGATACCTGATAATAATTTAATTGACTTCCAGTTATCGAATGATGTG
ATTCCTATCAAAATACAACCGAAAACGGCTGTAGAAACAATGCAATTAATAGCAGCTCCTAATATTAGATCTGTAGAAGA
ATTAATAAATCAGTTTAATTTGCCTATACACCAATTTGTAAAAACGATAATTGTTCGCGCTAAAAATAAACATATCAATA
ATGATTATTCTTTGTTAGGATTAGTCATTCGAGCTGATCATCAAATTAGTTTAAAAAAAATTGCGATGATTCCACAAATA
CATATCCCTTTATCTTGTATCGAGCCAGAAGAAATCCAAAAAATTACCGGAGCACAGCCCAATTTGTTGGGTCCTATTAA
CTTGTCTATACCATTAATTATAGATTATAATGTAGCAAAAATGAACGATTTTGTCGCCGGATCTAATATGAGCGGCAAAT
ATTTTTTTGGGGTTAATTGGAATCGTGATATACTATTTTCAAAAGTAGCGGATTTACATGAAGTGTTGCATCATAATTCG
TATACTAATAAAAAAAATATCTTATCCATTCATAACTGTATTGAAATAGGACACATATTTCAATTAGGACAAAAATACTT
AAACTTGCATACTAATTATTCACAAAAAAATAATGAGCATAAACATAATTTATCAATGGAAATGGGTTGTTACGGTATAG
GAATTACTCGTATTATTGCTGTGATTATTGAACAAAATTATGATAAAAATGGTATTTTATGGCCGGATGTAATCGCACCA
TTCAAATTAGCGATCATACCAATTGATATGTATCGTTCTGTTAATGTAAGAAATATTGCAGAAAAAATTTACACACAACT
GTTATCTGTTATAGGAATAGATATATTAATTGATGATCGTAAAGAATACCCAGGTACAATGTTTGCTGATATAGATTTGA
TCGGAATACCACATATTCTGATTATTAGTGATCGTAGTTTAGCTAATCAAGAGGTAGAATATAAATATCGTAAAAAAAAT
AAAATAGAAAAAATTAAACTTGAAATGTTAATACGTTATTTGATTGAAAAAATTGTTTCTTCATGA

Upstream 100 bases:

>100_bases
AATCAAGAATTTAATTCTTTAATTATAAATATACTTTAACGACGATTATTATTATTATTTTTATAATTACTAATAAACTT
TTTCATATCAGGCAAAAATA

Downstream 100 bases:

>100_bases
GATTTTTTAATCAATTTGGATTGATAAAATAATGTTGATATTAAAAAATTTCAGTGATATATAAAGAATATATATATGTA
ATAGTTATGTTGATTGAAGT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MRTSQYLLATLKEAPKNCKAISHQLMLRAGLIRQVSSGLYTWLPTGLRVLRKIENIIREEMSKIGAIEIAMPIVQPARLW
KKSGRWTGYGTELLRFKNRNNQEFVLGPTHEEMVSEIICKETMLYKQFPLIVYQIHTKYRDEARPRSGVIRAREFIMKDG
YSFHINQKSLQNTYNNMYQTYHTIFNRIGLNFCVVQAEPGKIGGALSHEFQAYSENGEDSIAVPMIPDNNLIDFQLSNDV
IPIKIQPKTAVETMQLIAAPNIRSVEELINQFNLPIHQFVKTIIVRAKNKHINNDYSLLGLVIRADHQISLKKIAMIPQI
HIPLSCIEPEEIQKITGAQPNLLGPINLSIPLIIDYNVAKMNDFVAGSNMSGKYFFGVNWNRDILFSKVADLHEVLHHNS
YTNKKNILSIHNCIEIGHIFQLGQKYLNLHTNYSQKNNEHKHNLSMEMGCYGIGITRIIAVIIEQNYDKNGILWPDVIAP
FKLAIIPIDMYRSVNVRNIAEKIYTQLLSVIGIDILIDDRKEYPGTMFADIDLIGIPHILIISDRSLANQEVEYKYRKKN
KIEKIKLEMLIRYLIEKIVSS

Sequences:

>Translated_581_residues
MRTSQYLLATLKEAPKNCKAISHQLMLRAGLIRQVSSGLYTWLPTGLRVLRKIENIIREEMSKIGAIEIAMPIVQPARLW
KKSGRWTGYGTELLRFKNRNNQEFVLGPTHEEMVSEIICKETMLYKQFPLIVYQIHTKYRDEARPRSGVIRAREFIMKDG
YSFHINQKSLQNTYNNMYQTYHTIFNRIGLNFCVVQAEPGKIGGALSHEFQAYSENGEDSIAVPMIPDNNLIDFQLSNDV
IPIKIQPKTAVETMQLIAAPNIRSVEELINQFNLPIHQFVKTIIVRAKNKHINNDYSLLGLVIRADHQISLKKIAMIPQI
HIPLSCIEPEEIQKITGAQPNLLGPINLSIPLIIDYNVAKMNDFVAGSNMSGKYFFGVNWNRDILFSKVADLHEVLHHNS
YTNKKNILSIHNCIEIGHIFQLGQKYLNLHTNYSQKNNEHKHNLSMEMGCYGIGITRIIAVIIEQNYDKNGILWPDVIAP
FKLAIIPIDMYRSVNVRNIAEKIYTQLLSVIGIDILIDDRKEYPGTMFADIDLIGIPHILIISDRSLANQEVEYKYRKKN
KIEKIKLEMLIRYLIEKIVSS
>Mature_581_residues
MRTSQYLLATLKEAPKNCKAISHQLMLRAGLIRQVSSGLYTWLPTGLRVLRKIENIIREEMSKIGAIEIAMPIVQPARLW
KKSGRWTGYGTELLRFKNRNNQEFVLGPTHEEMVSEIICKETMLYKQFPLIVYQIHTKYRDEARPRSGVIRAREFIMKDG
YSFHINQKSLQNTYNNMYQTYHTIFNRIGLNFCVVQAEPGKIGGALSHEFQAYSENGEDSIAVPMIPDNNLIDFQLSNDV
IPIKIQPKTAVETMQLIAAPNIRSVEELINQFNLPIHQFVKTIIVRAKNKHINNDYSLLGLVIRADHQISLKKIAMIPQI
HIPLSCIEPEEIQKITGAQPNLLGPINLSIPLIIDYNVAKMNDFVAGSNMSGKYFFGVNWNRDILFSKVADLHEVLHHNS
YTNKKNILSIHNCIEIGHIFQLGQKYLNLHTNYSQKNNEHKHNLSMEMGCYGIGITRIIAVIIEQNYDKNGILWPDVIAP
FKLAIIPIDMYRSVNVRNIAEKIYTQLLSVIGIDILIDDRKEYPGTMFADIDLIGIPHILIISDRSLANQEVEYKYRKKN
KIEKIKLEMLIRYLIEKIVSS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=215, Percent_Identity=42.7906976744186, Blast_Score=198, Evalue=1e-50,
Organism=Escherichia coli, GI1786392, Length=578, Percent_Identity=50.1730103806228, Blast_Score=632, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=212, Percent_Identity=41.0377358490566, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI193203271, Length=93, Percent_Identity=36.5591397849462, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=201, Percent_Identity=37.3134328358209, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24656200, Length=201, Percent_Identity=35.3233830845771, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_BLOPB (Q493B4)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277801.1
- ProteinModelPortal:   Q493B4
- SMR:   Q493B4
- STRING:   Q493B4
- GeneID:   3562842
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_297
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   CBLO291272:BPEN_297-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 66749; Mature: 66749

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQYLLATLKEAPKNCKAISHQLMLRAGLIRQVSSGLYTWLPTGLRVLRKIENIIREE
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MSKIGAIEIAMPIVQPARLWKKSGRWTGYGTELLRFKNRNNQEFVLGPTHEEMVSEIICK
HHHCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEECCCHHHHHHHHHHH
ETMLYKQFPLIVYQIHTKYRDEARPRSGVIRAREFIMKDGYSFHINQKSLQNTYNNMYQT
HHHHHHHCCEEEEEEEHHHHHCCCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHH
YHTIFNRIGLNFCVVQAEPGKIGGALSHEFQAYSENGEDSIAVPMIPDNNLIDFQLSNDV
HHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCE
IPIKIQPKTAVETMQLIAAPNIRSVEELINQFNLPIHQFVKTIIVRAKNKHINNDYSLLG
EEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHEEE
LVIRADHQISLKKIAMIPQIHIPLSCIEPEEIQKITGAQPNLLGPINLSIPLIIDYNVAK
EEEECCCCCCHHHHHCCCEEECCCCCCCHHHHHHHCCCCCCEECCCCCEEEEEEECCHHH
MNDFVAGSNMSGKYFFGVNWNRDILFSKVADLHEVLHHNSYTNKKNILSIHNCIEIGHIF
HHHEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
QLGQKYLNLHTNYSQKNNEHKHNLSMEMGCYGIGITRIIAVIIEQNYDKNGILWPDVIAP
HHHHHHHHHCCCCCCCCCCCHHCCCEECCEEHHHHHHHHHHHHHCCCCCCCCCCCHHHCC
FKLAIIPIDMYRSVNVRNIAEKIYTQLLSVIGIDILIDDRKEYPGTMFADIDLIGIPHIL
CEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEEECCCEEE
IISDRSLANQEVEYKYRKKNKIEKIKLEMLIRYLIEKIVSS
EECCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTSQYLLATLKEAPKNCKAISHQLMLRAGLIRQVSSGLYTWLPTGLRVLRKIENIIREE
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MSKIGAIEIAMPIVQPARLWKKSGRWTGYGTELLRFKNRNNQEFVLGPTHEEMVSEIICK
HHHCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEECCCHHHHHHHHHHH
ETMLYKQFPLIVYQIHTKYRDEARPRSGVIRAREFIMKDGYSFHINQKSLQNTYNNMYQT
HHHHHHHCCEEEEEEEHHHHHCCCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHH
YHTIFNRIGLNFCVVQAEPGKIGGALSHEFQAYSENGEDSIAVPMIPDNNLIDFQLSNDV
HHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCE
IPIKIQPKTAVETMQLIAAPNIRSVEELINQFNLPIHQFVKTIIVRAKNKHINNDYSLLG
EEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHEEE
LVIRADHQISLKKIAMIPQIHIPLSCIEPEEIQKITGAQPNLLGPINLSIPLIIDYNVAK
EEEECCCCCCHHHHHCCCEEECCCCCCCHHHHHHHCCCCCCEECCCCCEEEEEEECCHHH
MNDFVAGSNMSGKYFFGVNWNRDILFSKVADLHEVLHHNSYTNKKNILSIHNCIEIGHIF
HHHEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
QLGQKYLNLHTNYSQKNNEHKHNLSMEMGCYGIGITRIIAVIIEQNYDKNGILWPDVIAP
HHHHHHHHHCCCCCCCCCCCHHCCCEECCEEHHHHHHHHHHHHHCCCCCCCCCCCHHHCC
FKLAIIPIDMYRSVNVRNIAEKIYTQLLSVIGIDILIDDRKEYPGTMFADIDLIGIPHIL
CEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEEECCCEEE
IISDRSLANQEVEYKYRKKNKIEKIKLEMLIRYLIEKIVSS
EECCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA