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The map label for this gene is murD

Identifier: 68535823

GI number: 68535823

Start: 893453

End: 894961

Strand: Direct

Name: murD

Synonym: jk0748

Alternate gene names: 68535823

Gene position: 893453-894961 (Clockwise)

Preceding gene: 68535822

Following gene: 68535824

Centisome position: 36.28

GC content: 66.0

Gene sequence:

>1509_bases
GTGGAATCGACACACGCACTCCAACCCGCAGAAGCCCTAGAAACCATGCGCTCTCAGACCGTTCTGGTCGCGGGCGCGGG
CGTGGCCGGGCGGGGCGTGATCTCCATGCTCTGCGCCCTCGGTGCCCGGTTGGTCCTGGTTGCAGACGACAACGCGCAGG
CGCTTGGCCACATCGCGGATGATGGCGATACAGAGCTGCGGCTGCTGAGTGTTTCCGACGCCATCGATACCCTCGAAGAG
TTGGCCCCACAGTTGGTCGTGACCTCCCCGGGTTGGAAGCCCGAATCCCCGCTGCTGGCCCGTGCCGCCGAGCTGAACAT
CCCGGTGATCGGCGACATTGCGGCGGCCTGGCTGGCCGACCAAGCCGGTGCCTTCGGCCAGCCGCGCACGTGGCTGGCTG
TCACCGGGACAAACGGTAAGACAACCACCACGGCAATGCTGACTTCGATGCTGATCGCTGACGGGCGTGCAGCCCTGGCT
GTGGGAAACATTGGCATTGCCCCTTCCGCTGCCTTGGCTGCTCAGCACCGCGGAGAACCCCGCTCCGATGTCTTCGTCGC
CGAGGTGTCGAGCTTCCAGCTGCATTGGGCGCCCATGTTCAAGCCCACCGTCGGTTGCATTCTGAACCTTGCCGAAGACC
ACCTGGATTGGCATGGCAGCTACGAAAACTATTGTGCAGACAAGGCACAAGTCTTGACTGCTGAGGAATCCGTGCTGGCT
CTGGATGACGACGACGTGCTGACCACCGCACGCGCACGCGAGGTAGTGGCGCGGCGTGGGTACACGATTCACGATCCTGA
AGAGGTGGCGTCACAAAACACGGAACGAGTAGTCGGCGTCCGCGACGGGCGCCTGGTCGAGGTCGTCATCGGCGACCCGA
GTCAGACGACGGACCTCGCTCCGGCACAGGGCATCAGCCCTCCCGGGCCGGCGGGGCTGGCAGACGCCGCGGCGAGTGCG
GCGATGGCGCGCGCCCTGGGCGTGGCTCCGGGGGCCATCGAGGCCGCGCTGGCTGGATTCAAGGTGCAAGCACACCGCGG
ACAGGTCGTGCTGGAGCACGGAGGAGTTACCTGGATCGATAACTCGAAGGCCACCAACCCGCACGCTGCCGAAGCCGCGC
TGCGTGGCCAGCGCAACGTGATCTGGGTCGCTGGCGGACAGCTGAAGGGGGCTGCGGTCGACGGGTTGATCCGGGCGATC
GGTGGTGCCCTGAAGGCAGTGGTGGCTCTGGGGGTGGATCGCGCGGAGCTCGTCGCGGAAGTGTCGCGGCAGCTGCCCGA
CCTGCCGGTCACTGTTATTGACGCCACCGATCCGGAAGAGGCGATGCGTGCTGTCGCCCGCGCCGCCCATGGGCTGGCGC
AACCCGGTGACAGCGTTGTGCTGGCCCCGGCAGCGGCCTCACTGGATATGTACACAGGCATGAGCCAGCGTGGCGACCTG
TTCGCCCAGTATGCGGTGGCATATGCTGGGGCCGATAGAGCCGATGAACGCACTCAGGAAAGGGGATAG

Upstream 100 bases:

>100_bases
TTTTCTACGCGGAGTGGCTCAACGGCGCCAGCTTCTAGGCGGCTGCCGGTGGCAGACCCACAAACCCACTATTCGCTAGC
AATGTAAGGACAACCCAGCC

Downstream 100 bases:

>100_bases
GCGCGATGTCCACTCCGACGCCCCATTCGAAGCGCCGGCGCATCCCCGGCACCGGGGAGGCCAGCCGCCACGGCAACCGC
GCCGACCACTCTGACCGTGC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MESTHALQPAEALETMRSQTVLVAGAGVAGRGVISMLCALGARLVLVADDNAQALGHIADDGDTELRLLSVSDAIDTLEE
LAPQLVVTSPGWKPESPLLARAAELNIPVIGDIAAAWLADQAGAFGQPRTWLAVTGTNGKTTTTAMLTSMLIADGRAALA
VGNIGIAPSAALAAQHRGEPRSDVFVAEVSSFQLHWAPMFKPTVGCILNLAEDHLDWHGSYENYCADKAQVLTAEESVLA
LDDDDVLTTARAREVVARRGYTIHDPEEVASQNTERVVGVRDGRLVEVVIGDPSQTTDLAPAQGISPPGPAGLADAAASA
AMARALGVAPGAIEAALAGFKVQAHRGQVVLEHGGVTWIDNSKATNPHAAEAALRGQRNVIWVAGGQLKGAAVDGLIRAI
GGALKAVVALGVDRAELVAEVSRQLPDLPVTVIDATDPEEAMRAVARAAHGLAQPGDSVVLAPAAASLDMYTGMSQRGDL
FAQYAVAYAGADRADERTQERG

Sequences:

>Translated_502_residues
MESTHALQPAEALETMRSQTVLVAGAGVAGRGVISMLCALGARLVLVADDNAQALGHIADDGDTELRLLSVSDAIDTLEE
LAPQLVVTSPGWKPESPLLARAAELNIPVIGDIAAAWLADQAGAFGQPRTWLAVTGTNGKTTTTAMLTSMLIADGRAALA
VGNIGIAPSAALAAQHRGEPRSDVFVAEVSSFQLHWAPMFKPTVGCILNLAEDHLDWHGSYENYCADKAQVLTAEESVLA
LDDDDVLTTARAREVVARRGYTIHDPEEVASQNTERVVGVRDGRLVEVVIGDPSQTTDLAPAQGISPPGPAGLADAAASA
AMARALGVAPGAIEAALAGFKVQAHRGQVVLEHGGVTWIDNSKATNPHAAEAALRGQRNVIWVAGGQLKGAAVDGLIRAI
GGALKAVVALGVDRAELVAEVSRQLPDLPVTVIDATDPEEAMRAVARAAHGLAQPGDSVVLAPAAASLDMYTGMSQRGDL
FAQYAVAYAGADRADERTQERG
>Mature_502_residues
MESTHALQPAEALETMRSQTVLVAGAGVAGRGVISMLCALGARLVLVADDNAQALGHIADDGDTELRLLSVSDAIDTLEE
LAPQLVVTSPGWKPESPLLARAAELNIPVIGDIAAAWLADQAGAFGQPRTWLAVTGTNGKTTTTAMLTSMLIADGRAALA
VGNIGIAPSAALAAQHRGEPRSDVFVAEVSSFQLHWAPMFKPTVGCILNLAEDHLDWHGSYENYCADKAQVLTAEESVLA
LDDDDVLTTARAREVVARRGYTIHDPEEVASQNTERVVGVRDGRLVEVVIGDPSQTTDLAPAQGISPPGPAGLADAAASA
AMARALGVAPGAIEAALAGFKVQAHRGQVVLEHGGVTWIDNSKATNPHAAEAALRGQRNVIWVAGGQLKGAAVDGLIRAI
GGALKAVVALGVDRAELVAEVSRQLPDLPVTVIDATDPEEAMRAVARAAHGLAQPGDSVVLAPAAASLDMYTGMSQRGDL
FAQYAVAYAGADRADERTQERG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=413, Percent_Identity=31.4769975786925, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_CORJK (Q4JW97)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250528.1
- ProteinModelPortal:   Q4JW97
- SMR:   Q4JW97
- STRING:   Q4JW97
- GeneID:   3432774
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0748
- NMPDR:   fig|306537.3.peg.1607
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK00141
- BioCyc:   CJEI306537:JK0748-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 51982; Mature: 51982

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTHALQPAEALETMRSQTVLVAGAGVAGRGVISMLCALGARLVLVADDNAQALGHIAD
CCCCCCCCHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCHHHCCCCC
DGDTELRLLSVSDAIDTLEELAPQLVVTSPGWKPESPLLARAAELNIPVIGDIAAAWLAD
CCCCEEEEEEHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHCCCCEEHHHHHHHHHH
QAGAFGQPRTWLAVTGTNGKTTTTAMLTSMLIADGRAALAVGNIGIAPSAALAAQHRGEP
HCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCC
RSDVFVAEVSSFQLHWAPMFKPTVGCILNLAEDHLDWHGSYENYCADKAQVLTAEESVLA
CCCEEEEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEECCCEEE
LDDDDVLTTARAREVVARRGYTIHDPEEVASQNTERVVGVRDGRLVEVVIGDPSQTTDLA
ECCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCC
PAQGISPPGPAGLADAAASAAMARALGVAPGAIEAALAGFKVQAHRGQVVLEHGGVTWID
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEECCCCEEEECCCEEEEC
NSKATNPHAAEAALRGQRNVIWVAGGQLKGAAVDGLIRAIGGALKAVVALGVDRAELVAE
CCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VSRQLPDLPVTVIDATDPEEAMRAVARAAHGLAQPGDSVVLAPAAASLDMYTGMSQRGDL
HHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHCCCCCCCHH
FAQYAVAYAGADRADERTQERG
HHHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure
MESTHALQPAEALETMRSQTVLVAGAGVAGRGVISMLCALGARLVLVADDNAQALGHIAD
CCCCCCCCHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCHHHCCCCC
DGDTELRLLSVSDAIDTLEELAPQLVVTSPGWKPESPLLARAAELNIPVIGDIAAAWLAD
CCCCEEEEEEHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHCCCCEEHHHHHHHHHH
QAGAFGQPRTWLAVTGTNGKTTTTAMLTSMLIADGRAALAVGNIGIAPSAALAAQHRGEP
HCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCC
RSDVFVAEVSSFQLHWAPMFKPTVGCILNLAEDHLDWHGSYENYCADKAQVLTAEESVLA
CCCEEEEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEECCCEEE
LDDDDVLTTARAREVVARRGYTIHDPEEVASQNTERVVGVRDGRLVEVVIGDPSQTTDLA
ECCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCC
PAQGISPPGPAGLADAAASAAMARALGVAPGAIEAALAGFKVQAHRGQVVLEHGGVTWID
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEECCCCEEEECCCEEEEC
NSKATNPHAAEAALRGQRNVIWVAGGQLKGAAVDGLIRAIGGALKAVVALGVDRAELVAE
CCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VSRQLPDLPVTVIDATDPEEAMRAVARAAHGLAQPGDSVVLAPAAASLDMYTGMSQRGDL
HHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHCCCCCCCHH
FAQYAVAYAGADRADERTQERG
HHHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA