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The map label for this gene is murE [H]

Identifier: 68535820

GI number: 68535820

Start: 889152

End: 890762

Strand: Direct

Name: murE [H]

Synonym: jk0745

Alternate gene names: 68535820

Gene position: 889152-890762 (Clockwise)

Preceding gene: 68535819

Following gene: 68535821

Centisome position: 36.11

GC content: 62.57

Gene sequence:

>1611_bases
ATGACATCGTTGAAGCAGCTCGCTGAAATCTGCGGAGGCACCCTCACTGGGCCTTGGGAAGGCGTTGAGGTGACCTCCGC
CGCGATTGGTTCTCAAGATGTGGAGCAGGGTGGTTTGTTCTGCGCCGTTCCCGGACAGAAGGTCCACGGCGCGACCTTCG
CCGCGGATAGCAAAGCCGCCGCTGTTCTGACCGATGCCGCAGGCCAGCGCATTATTGATGACGCCACCACGCCGCTACCG
TGCATCGTTGTCGACAACGTTCGCAAGTGGATGGGGCCGGTAGCTGCAGAGGTTTATGGCCACCCGGCGCGTCAGTTGAA
GACCATCGGGATTACCGGTACGTCGGGCAAGACCACCACCAGCTACTTGGTGGAAAAGGCTCTTCTGGAATCCGGACTGC
GGGTGGGCCTGATCGGCACCACGGGTACACGCATCAACGGCCAGCCCATCCCTACCTCACTGACTACTCCCGAGGCACCC
ACGCTGCAGGCCTTGCTGGCACGAATGGTGGCCGAGGGAGTCACGCACCTCGTGATGGAGGTTTCCAGCCACGCATTGCA
GCTAGGCCGCGTGCACGGCGTGCACTTTGACGTGGTGGCGTTTACGAACCTGAGCCAGGACCACCTGGATTTTCATCCGA
CGATGGAGGACTACTTCCAGGCGAAAGCGCTGCTGTTCGAACGCGCGGAGGATGGTGGTGTTCCGGGCACCGCTGTGGTC
TGTTGTGAGGACGAGTGGGGCGAGCGCATGCGCGCCATCGCCGCGAAGTACTCGTCGACCGTGGCGCTGTACCCCCAGGG
CGGCAACCACGAGGCCGAAACCGGCAGCCCCGTGTGGTCTATTGCTTCCGCCGAGGTGGCTCCCAACGGCCGCCAGCACA
TTCGCCTGTCTATCGCGGGCGGCTCCGAACCGCGAGAGTTGGATTACAGCATCGGCATGGCGGGTTCCTTTAACCAGGCC
AACAGCCTGGTGGCTCTGGCCTGCATCGAGGCAGTCACTGGAGAACCGCTGTCTAGCGATAGCACAGCCGTACAGGCTCT
GGCGGACGTGCAGGTTCCCGGCCGGATGCAGCTGGTCGAGGAAGGCCAAGACTTCCTGGCCATGGTTGACTATGCGCACA
AGCCGGGCGCGGTGCAGGCCGTGCTGGGCAGCTTGCACGACTATCTGCCGGATCCGGCAGCCCGCATTGGCATCGTCCTC
GGAGCCGGTGGAAACCGCGATCACGACAAGCGCGCGATCATGGGCCGTATCGCCGCGGAGTTGGCGGATGCGGTGTTCGT
CACGGATGACAATCCCCGGGATGAGGAACCTTCCGTGATTAGGGAAGCTATTTACGCCGGGGCGCGAGAGGGCGTCCAAA
AGAAAGAACAAGAACAATCAACCAACGCCGAGACCGTGTGCGAGATCGTGCCCGACCGTGCGGAGGCCATCGCCGCAGCC
GTAACCTGGGCACGGCCCGGCGATGCGGTGGTCGTCGCCGGTAAGGGGCATGAAAAGGGGCAACTTGTGGCAGGAAAGAT
GCATCCCTTCGACGATGTGGAGGAGTTGGCGACGGCTTTGAAGAACCGGGTACGGCAAGAGCGTGAGGCAGATAATGGTG
AAGGTAGGTAG

Upstream 100 bases:

>100_bases
CTGCACCTTTGTTCCACGACATCATGGCGTGGATGCTCGACCACTACAATGTGCCGCTGTCTGAGAAGGCAGGGCCAAAG
CTGATGCTGGAGGCAAATCA

Downstream 100 bases:

>100_bases
CAGCGATGATTGAGTTAACGACACAGAAGATCGCCGAGGTATCCGGCAGCACGCTGATTAACGACGTGGATCCGCAGGCC
GTAGTGACCGGCCCGGTGGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MTSLKQLAEICGGTLTGPWEGVEVTSAAIGSQDVEQGGLFCAVPGQKVHGATFAADSKAAAVLTDAAGQRIIDDATTPLP
CIVVDNVRKWMGPVAAEVYGHPARQLKTIGITGTSGKTTTSYLVEKALLESGLRVGLIGTTGTRINGQPIPTSLTTPEAP
TLQALLARMVAEGVTHLVMEVSSHALQLGRVHGVHFDVVAFTNLSQDHLDFHPTMEDYFQAKALLFERAEDGGVPGTAVV
CCEDEWGERMRAIAAKYSSTVALYPQGGNHEAETGSPVWSIASAEVAPNGRQHIRLSIAGGSEPRELDYSIGMAGSFNQA
NSLVALACIEAVTGEPLSSDSTAVQALADVQVPGRMQLVEEGQDFLAMVDYAHKPGAVQAVLGSLHDYLPDPAARIGIVL
GAGGNRDHDKRAIMGRIAAELADAVFVTDDNPRDEEPSVIREAIYAGAREGVQKKEQEQSTNAETVCEIVPDRAEAIAAA
VTWARPGDAVVVAGKGHEKGQLVAGKMHPFDDVEELATALKNRVRQEREADNGEGR

Sequences:

>Translated_536_residues
MTSLKQLAEICGGTLTGPWEGVEVTSAAIGSQDVEQGGLFCAVPGQKVHGATFAADSKAAAVLTDAAGQRIIDDATTPLP
CIVVDNVRKWMGPVAAEVYGHPARQLKTIGITGTSGKTTTSYLVEKALLESGLRVGLIGTTGTRINGQPIPTSLTTPEAP
TLQALLARMVAEGVTHLVMEVSSHALQLGRVHGVHFDVVAFTNLSQDHLDFHPTMEDYFQAKALLFERAEDGGVPGTAVV
CCEDEWGERMRAIAAKYSSTVALYPQGGNHEAETGSPVWSIASAEVAPNGRQHIRLSIAGGSEPRELDYSIGMAGSFNQA
NSLVALACIEAVTGEPLSSDSTAVQALADVQVPGRMQLVEEGQDFLAMVDYAHKPGAVQAVLGSLHDYLPDPAARIGIVL
GAGGNRDHDKRAIMGRIAAELADAVFVTDDNPRDEEPSVIREAIYAGAREGVQKKEQEQSTNAETVCEIVPDRAEAIAAA
VTWARPGDAVVVAGKGHEKGQLVAGKMHPFDDVEELATALKNRVRQEREADNGEGR
>Mature_535_residues
TSLKQLAEICGGTLTGPWEGVEVTSAAIGSQDVEQGGLFCAVPGQKVHGATFAADSKAAAVLTDAAGQRIIDDATTPLPC
IVVDNVRKWMGPVAAEVYGHPARQLKTIGITGTSGKTTTSYLVEKALLESGLRVGLIGTTGTRINGQPIPTSLTTPEAPT
LQALLARMVAEGVTHLVMEVSSHALQLGRVHGVHFDVVAFTNLSQDHLDFHPTMEDYFQAKALLFERAEDGGVPGTAVVC
CEDEWGERMRAIAAKYSSTVALYPQGGNHEAETGSPVWSIASAEVAPNGRQHIRLSIAGGSEPRELDYSIGMAGSFNQAN
SLVALACIEAVTGEPLSSDSTAVQALADVQVPGRMQLVEEGQDFLAMVDYAHKPGAVQAVLGSLHDYLPDPAARIGIVLG
AGGNRDHDKRAIMGRIAAELADAVFVTDDNPRDEEPSVIREAIYAGAREGVQKKEQEQSTNAETVCEIVPDRAEAIAAAV
TWARPGDAVVVAGKGHEKGQLVAGKMHPFDDVEELATALKNRVRQEREADNGEGR

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=489, Percent_Identity=34.560327198364, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 56557; Mature: 56426

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLKQLAEICGGTLTGPWEGVEVTSAAIGSQDVEQGGLFCAVPGQKVHGATFAADSKAA
CCCHHHHHHHHCCCCCCCCCCCEEHHHHHCCCHHHHCCEEEECCCCCCCCCEEECCCCCC
AVLTDAAGQRIIDDATTPLPCIVVDNVRKWMGPVAAEVYGHPARQLKTIGITGTSGKTTT
EEEEHHCCCHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCHH
SYLVEKALLESGLRVGLIGTTGTRINGQPIPTSLTTPEAPTLQALLARMVAEGVTHLVME
HHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VSSHALQLGRVHGVHFDVVAFTNLSQDHLDFHPTMEDYFQAKALLFERAEDGGVPGTAVV
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEE
CCEDEWGERMRAIAAKYSSTVALYPQGGNHEAETGSPVWSIASAEVAPNGRQHIRLSIAG
EECHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEC
GSEPRELDYSIGMAGSFNQANSLVALACIEAVTGEPLSSDSTAVQALADVQVPGRMQLVE
CCCCCEECEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHH
EGQDFLAMVDYAHKPGAVQAVLGSLHDYLPDPAARIGIVLGAGGNRDHDKRAIMGRIAAE
CCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHH
LADAVFVTDDNPRDEEPSVIREAIYAGAREGVQKKEQEQSTNAETVCEIVPDRAEAIAAA
HHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHH
VTWARPGDAVVVAGKGHEKGQLVAGKMHPFDDVEELATALKNRVRQEREADNGEGR
HEECCCCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TSLKQLAEICGGTLTGPWEGVEVTSAAIGSQDVEQGGLFCAVPGQKVHGATFAADSKAA
CCHHHHHHHHCCCCCCCCCCCEEHHHHHCCCHHHHCCEEEECCCCCCCCCEEECCCCCC
AVLTDAAGQRIIDDATTPLPCIVVDNVRKWMGPVAAEVYGHPARQLKTIGITGTSGKTTT
EEEEHHCCCHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCHH
SYLVEKALLESGLRVGLIGTTGTRINGQPIPTSLTTPEAPTLQALLARMVAEGVTHLVME
HHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VSSHALQLGRVHGVHFDVVAFTNLSQDHLDFHPTMEDYFQAKALLFERAEDGGVPGTAVV
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEE
CCEDEWGERMRAIAAKYSSTVALYPQGGNHEAETGSPVWSIASAEVAPNGRQHIRLSIAG
EECHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEC
GSEPRELDYSIGMAGSFNQANSLVALACIEAVTGEPLSSDSTAVQALADVQVPGRMQLVE
CCCCCEECEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHH
EGQDFLAMVDYAHKPGAVQAVLGSLHDYLPDPAARIGIVLGAGGNRDHDKRAIMGRIAAE
CCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHH
LADAVFVTDDNPRDEEPSVIREAIYAGAREGVQKKEQEQSTNAETVCEIVPDRAEAIAAA
HHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHH
VTWARPGDAVVVAGKGHEKGQLVAGKMHPFDDVEELATALKNRVRQEREADNGEGR
HEECCCCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]