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The map label for this gene is murF [H]

Identifier: 68535821

GI number: 68535821

Start: 890768

End: 892282

Strand: Direct

Name: murF [H]

Synonym: jk0746

Alternate gene names: 68535821

Gene position: 890768-892282 (Clockwise)

Preceding gene: 68535820

Following gene: 68535822

Centisome position: 36.17

GC content: 64.55

Gene sequence:

>1515_bases
ATGATTGAGTTAACGACACAGAAGATCGCCGAGGTATCCGGCAGCACGCTGATTAACGACGTGGATCCGCAGGCCGTAGT
GACCGGCCCGGTGGAGTTCGACTCGCGCAAGATCACACCGGGCGCGATCTTCATGGCTCTGCCAGGTGCACGTGCCGACG
GCCACGACTTCGCCGATGCAGCTCTGAACAGCGGCGCCGCGCTGCTGATCGTTGGCCGCCCGGTGGAGCAGCCCGCCCTG
CTGGCCGCCCCGGTGGAGATCAACGAACAAACCTCCAATGCCACCGCATTCGAGTTCGACGCGGAAGGTCACGGCGCAGC
AGTGCTGGCAGCGATCGATAAGCTCGCGCGCTATAACACCGACGTGCTCGCTGCCGAGGAGGGCATGGTCGTCGTCGGCG
TGACCGGCTCTGCGGGCAAGACCTCCACGAAGGATCTGATTGGCGCGGTGCTGCGCGAGGCAGGGGAGACCGTCGCTCCG
CCCGGCAGTTTCAACAACGAGATCGGCCTGCCTTACACAGCCCTGCGTGCGGGCCGCGACACGCGCTTCCTCGTTAGCGA
GATGTCCGCCCGTGGCGTAGGCCACATCCAGCACTTGACGACCGTTACCCCGCCGCGAGTGGGTGTGGTGCTGAACGTCG
GCAGTGCCCACCTGGGCGAGTTCGGCTCCCGCCAGGCCATCGCGCAGGCGAAGGGCGAGCTGGTGGAGGCTCTGCCGGCT
GCCGCTGATGGGGGCCTGGCCGTGCTGAACGCAGACGACGACAAGGTTGCGGCGATGGATAAGCGCACCGCCGCGCGCGT
CGTGCGTTTTTCCACGGAAGGCGCGACGGGGGCAGCGGGCGTGCCGGCGGATTACTATGCGACCGACATCGAGCTCGACG
CTGTAGCCCGCGCCAGCTTTATGCTGCACCACCCAGTAGGGGAGCCGGTGCGTGTGGAACTTTCGGTATTCGGCGCCCAC
CAGGTCTCGAACGCGCTCGCCGCCGCGGCGGTTGGTATCGAGATGGGTATTGACGCCGCCACGGTCGCATCCCTGCTGAG
CTCTCACGTTGCCGCTAGCGTAAACCGCATGGATGTGCGCACCCGCGAATCTGACGGAGTGACCATCATCAACGATTCTT
ATAACGCCAACCCCGAATCCATGCGCGCGGGAATCGACGCGCTGGCTTACACCGCCAAGGGCCGTCGCGATGCGGCGAGC
TGGGCCGTGCTGGGGCAGATGGGTGAGTTGGGCGAGGACGCTAGCGACGAGCACGCCGAGCTCGGCCACGTGCTGGCGCA
ACGACGCATCGACCACGCGGTGATCGTGGGCAACGGGGTGAATCAGCGTGCGCTGGCCAACGCTGCTCGAGAGAGCGGTG
TGGAAACCCAAGTCGTCGAAAACATCGACGGCGCGGTGAATTACCTAGATATGAGTTTGAATCCGCAGGATGTAGTGCTG
ATCAAGGCATCGTATTCGGACGGACTATGGGCAGTGGCTGAAGGACTGCTCATCGGTGAGGATCAAGGAGCTTAA

Upstream 100 bases:

>100_bases
AGATGCATCCCTTCGACGATGTGGAGGAGTTGGCGACGGCTTTGAAGAACCGGGTACGGCAAGAGCGTGAGGCAGATAAT
GGTGAAGGTAGGTAGCAGCG

Downstream 100 bases:

>100_bases
AAATCCCATGTTGCAAATCTTTATCGCAGGTGCGGTTGCTTTCCTTGTGTCCGTATTCCTGACCCCGGTGCTGATTCGGT
GGTTCTCCGCTGAGGGATTG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MIELTTQKIAEVSGSTLINDVDPQAVVTGPVEFDSRKITPGAIFMALPGARADGHDFADAALNSGAALLIVGRPVEQPAL
LAAPVEINEQTSNATAFEFDAEGHGAAVLAAIDKLARYNTDVLAAEEGMVVVGVTGSAGKTSTKDLIGAVLREAGETVAP
PGSFNNEIGLPYTALRAGRDTRFLVSEMSARGVGHIQHLTTVTPPRVGVVLNVGSAHLGEFGSRQAIAQAKGELVEALPA
AADGGLAVLNADDDKVAAMDKRTAARVVRFSTEGATGAAGVPADYYATDIELDAVARASFMLHHPVGEPVRVELSVFGAH
QVSNALAAAAVGIEMGIDAATVASLLSSHVAASVNRMDVRTRESDGVTIINDSYNANPESMRAGIDALAYTAKGRRDAAS
WAVLGQMGELGEDASDEHAELGHVLAQRRIDHAVIVGNGVNQRALANAARESGVETQVVENIDGAVNYLDMSLNPQDVVL
IKASYSDGLWAVAEGLLIGEDQGA

Sequences:

>Translated_504_residues
MIELTTQKIAEVSGSTLINDVDPQAVVTGPVEFDSRKITPGAIFMALPGARADGHDFADAALNSGAALLIVGRPVEQPAL
LAAPVEINEQTSNATAFEFDAEGHGAAVLAAIDKLARYNTDVLAAEEGMVVVGVTGSAGKTSTKDLIGAVLREAGETVAP
PGSFNNEIGLPYTALRAGRDTRFLVSEMSARGVGHIQHLTTVTPPRVGVVLNVGSAHLGEFGSRQAIAQAKGELVEALPA
AADGGLAVLNADDDKVAAMDKRTAARVVRFSTEGATGAAGVPADYYATDIELDAVARASFMLHHPVGEPVRVELSVFGAH
QVSNALAAAAVGIEMGIDAATVASLLSSHVAASVNRMDVRTRESDGVTIINDSYNANPESMRAGIDALAYTAKGRRDAAS
WAVLGQMGELGEDASDEHAELGHVLAQRRIDHAVIVGNGVNQRALANAARESGVETQVVENIDGAVNYLDMSLNPQDVVL
IKASYSDGLWAVAEGLLIGEDQGA
>Mature_504_residues
MIELTTQKIAEVSGSTLINDVDPQAVVTGPVEFDSRKITPGAIFMALPGARADGHDFADAALNSGAALLIVGRPVEQPAL
LAAPVEINEQTSNATAFEFDAEGHGAAVLAAIDKLARYNTDVLAAEEGMVVVGVTGSAGKTSTKDLIGAVLREAGETVAP
PGSFNNEIGLPYTALRAGRDTRFLVSEMSARGVGHIQHLTTVTPPRVGVVLNVGSAHLGEFGSRQAIAQAKGELVEALPA
AADGGLAVLNADDDKVAAMDKRTAARVVRFSTEGATGAAGVPADYYATDIELDAVARASFMLHHPVGEPVRVELSVFGAH
QVSNALAAAAVGIEMGIDAATVASLLSSHVAASVNRMDVRTRESDGVTIINDSYNANPESMRAGIDALAYTAKGRRDAAS
WAVLGQMGELGEDASDEHAELGHVLAQRRIDHAVIVGNGVNQRALANAARESGVETQVVENIDGAVNYLDMSLNPQDVVL
IKASYSDGLWAVAEGLLIGEDQGA

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=442, Percent_Identity=32.1266968325792, Blast_Score=159, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 52117; Mature: 52117

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELTTQKIAEVSGSTLINDVDPQAVVTGPVEFDSRKITPGAIFMALPGARADGHDFADA
CCEECHHHHHHCCCCCEEECCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHH
ALNSGAALLIVGRPVEQPALLAAPVEINEQTSNATAFEFDAEGHGAAVLAAIDKLARYNT
HHCCCCEEEEECCCCCCCCEEECCCEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
DVLAAEEGMVVVGVTGSAGKTSTKDLIGAVLREAGETVAPPGSFNNEIGLPYTALRAGRD
CEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCC
TRFLVSEMSARGVGHIQHLTTVTPPRVGVVLNVGSAHLGEFGSRQAIAQAKGELVEALPA
HHHHHHHHHCCCCCHHHHHEECCCCCEEEEEECCCHHHCCCCCHHHHHHHHHHHHHHHCC
AADGGLAVLNADDDKVAAMDKRTAARVVRFSTEGATGAAGVPADYYATDIELDAVARASF
CCCCCEEEEECCCCHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHE
MLHHPVGEPVRVELSVFGAHQVSNALAAAAVGIEMGIDAATVASLLSSHVAASVNRMDVR
EEECCCCCCEEEEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEE
TRESDGVTIINDSYNANPESMRAGIDALAYTAKGRRDAASWAVLGQMGELGEDASDEHAE
EECCCCEEEECCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCHHHHH
LGHVLAQRRIDHAVIVGNGVNQRALANAARESGVETQVVENIDGAVNYLDMSLNPQDVVL
HHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCEEEE
IKASYSDGLWAVAEGLLIGEDQGA
EEECCCCCHHHHHCCEEEECCCCC
>Mature Secondary Structure
MIELTTQKIAEVSGSTLINDVDPQAVVTGPVEFDSRKITPGAIFMALPGARADGHDFADA
CCEECHHHHHHCCCCCEEECCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHH
ALNSGAALLIVGRPVEQPALLAAPVEINEQTSNATAFEFDAEGHGAAVLAAIDKLARYNT
HHCCCCEEEEECCCCCCCCEEECCCEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
DVLAAEEGMVVVGVTGSAGKTSTKDLIGAVLREAGETVAPPGSFNNEIGLPYTALRAGRD
CEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCC
TRFLVSEMSARGVGHIQHLTTVTPPRVGVVLNVGSAHLGEFGSRQAIAQAKGELVEALPA
HHHHHHHHHCCCCCHHHHHEECCCCCEEEEEECCCHHHCCCCCHHHHHHHHHHHHHHHCC
AADGGLAVLNADDDKVAAMDKRTAARVVRFSTEGATGAAGVPADYYATDIELDAVARASF
CCCCCEEEEECCCCHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHE
MLHHPVGEPVRVELSVFGAHQVSNALAAAAVGIEMGIDAATVASLLSSHVAASVNRMDVR
EEECCCCCCEEEEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEE
TRESDGVTIINDSYNANPESMRAGIDALAYTAKGRRDAASWAVLGQMGELGEDASDEHAE
EECCCCEEEECCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCHHHHH
LGHVLAQRRIDHAVIVGNGVNQRALANAARESGVETQVVENIDGAVNYLDMSLNPQDVVL
HHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCEEEE
IKASYSDGLWAVAEGLLIGEDQGA
EEECCCCCHHHHHCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]