Click here to switch to the map view.

The map label for this gene is dosC [H]

Identifier: 66766762

GI number: 66766762

Start: 492063

End: 493685

Strand: Reverse

Name: dosC [H]

Synonym: XC_0420

Alternate gene names: 66766762

Gene position: 493685-492063 (Counterclockwise)

Preceding gene: 66766771

Following gene: 66766759

Centisome position: 9.59

GC content: 68.02

Gene sequence:

>1623_bases
ATGGCAGAACAGCCCGAACACCCCAACCGCCCACGTGGCGGGCTGCGCCGTCTGCTGCCATGGGGCGGTGCCGGCACCGC
GCGCCCGGCTACCTTGCCCAGCGTGCTGGGCGAGCGCGACGGCGCTGGATTGGCTGCACATCAGCTGGTGCCGGCCAAGG
CCAAGTCGGGCGCGCATGACCCCGCCGCTGCCACTGCGCTACTGATCCGCCTGTTTTCGCATGCCGCGCACCCGGAAGCC
TTGCTGCTGGCCTTTGCCGAAGGCATTGCCAGCCTGCATGGCGAGCTCGGCGACATGGGCGTACGCCTGAGTACGGCGTA
TGCCGCCGGCGACTGGCCCGGGTATGGGCGCGCCCTGCGCCAGCTGATCGACAAATACATCCGCACCATCGATACCGGCG
ATTCGCTGGCCGAAGGCCGTACCGAAGCCGAACAGCTGCGCGACCTGCTGCGGCACGCGCTGGGCAATGCACTGGCCACG
CTGCTGCAACGCAGCCCGGAGTTGGCCGAAGAAGCGCAGACGCTGGCCTCGGCACTGCGCCACTGGCGACCCGGCCACGA
ACTGATCACCCTGGAACAGCGGCTGCGCGAGCTCAGCCACCAGATCGGCTTGCGCGCCGAAGATGCCAGCGAGCAACAGA
ACCTGTTGCTGGGGTTATTCGACCTATTGTTGGAAAACGTCGGCGAACTCCTGGACGATCGCAGTTGGCTGCAGGGCCAG
ATCTCGGTGGTGCGGCAGTTGATCGCCGGGCCGATGGACGTGGGTTCGATCGAACAGGCGCGCGGCACCCTGCGCGAGGT
GATCTACAAGCAAGGCCTGCTCAAGCAGGGCATTGCCGACTCCAAGACCGCCATGCGCGAGATGATGGTGTCCTTCGTCG
ACCGGCTCGACGGCATGGCCACCAGCACAGGCGAATACCACGACCGCGTTGCCGGTTATTCGCAGGCGATCGGCGACGCT
CGCAGCATTCCCGACCTCAACCGCTTGCTGCAGGACGTGTTGCAGGACACCGCCGGCGTGCAGGCGCAGGCGCTGGCCGC
GCGCGACGAGCTACTGTCCGCGCGCCGCCAGGTGGAAGAAGCCGAGCACCGCATCGCCTCGCTGGAGCAGGAGCTCAAGG
ACGTCGCCGGGCTGGTGCGCGAAGACCAGCTCACCGGCGCCCTCAACCGGCGCGGCTTCGAAGAACTGTTCGAGCGCGAG
GCCACCCGCACCCAGCGCAGCGGGCAACCGCTGTGCGTGGCGATGCTGGACCTGGACGACTTCCGCCGCCTCAACGAGAC
CCATGGGCATGCCGGTGGCGACGCCGCGTTGCGCCACACCGTGGACGTGGCCAAGTCGGTGCTGCGCACCACCGATGCGA
TTGCGCGCTTCGGTGGCGAGGAATTCGTGTTGCTGTTGCCGGACTCGACCATCTTCGAGGCCAGCGCCGCGGTGATCCGC
CTGCAGCGCGCGCTCGCACAGCGCTCGTTGCTGCATGAGGACGTGCGCGTGTTCGTCAGCTTCAGCGGCGGCGTGGCCAT
GCGCCGCACCGACGAAGCCCAGGACGAGGTGATCCGCCGCGCCGACCGCGCCATGTACGACTCCAAGGCGGCCGGCAAGA
ACCGGGTGATCAGCGCCGACTAA

Upstream 100 bases:

>100_bases
TTACTGCGGTCGCGTCTGCAAACCGCGCACGCGCGTGCTCAAGGGCCCTGCACCAACGCCGATAACGTGGCGTTGGTACT
TCACCCTCGAGACCTGGCGC

Downstream 100 bases:

>100_bases
CTCCCCTGCCAACGCAGCTTCAGATAGTCCATGACCTGCCCGGTCTGGGGTTGGGCAATGAACCCTTGGCGAAACAGCAT
GCATCCACGTATCTGAGGCA

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MAEQPEHPNRPRGGLRRLLPWGGAGTARPATLPSVLGERDGAGLAAHQLVPAKAKSGAHDPAAATALLIRLFSHAAHPEA
LLLAFAEGIASLHGELGDMGVRLSTAYAAGDWPGYGRALRQLIDKYIRTIDTGDSLAEGRTEAEQLRDLLRHALGNALAT
LLQRSPELAEEAQTLASALRHWRPGHELITLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQGQ
ISVVRQLIAGPMDVGSIEQARGTLREVIYKQGLLKQGIADSKTAMREMMVSFVDRLDGMATSTGEYHDRVAGYSQAIGDA
RSIPDLNRLLQDVLQDTAGVQAQALAARDELLSARRQVEEAEHRIASLEQELKDVAGLVREDQLTGALNRRGFEELFERE
ATRTQRSGQPLCVAMLDLDDFRRLNETHGHAGGDAALRHTVDVAKSVLRTTDAIARFGGEEFVLLLPDSTIFEASAAVIR
LQRALAQRSLLHEDVRVFVSFSGGVAMRRTDEAQDEVIRRADRAMYDSKAAGKNRVISAD

Sequences:

>Translated_540_residues
MAEQPEHPNRPRGGLRRLLPWGGAGTARPATLPSVLGERDGAGLAAHQLVPAKAKSGAHDPAAATALLIRLFSHAAHPEA
LLLAFAEGIASLHGELGDMGVRLSTAYAAGDWPGYGRALRQLIDKYIRTIDTGDSLAEGRTEAEQLRDLLRHALGNALAT
LLQRSPELAEEAQTLASALRHWRPGHELITLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQGQ
ISVVRQLIAGPMDVGSIEQARGTLREVIYKQGLLKQGIADSKTAMREMMVSFVDRLDGMATSTGEYHDRVAGYSQAIGDA
RSIPDLNRLLQDVLQDTAGVQAQALAARDELLSARRQVEEAEHRIASLEQELKDVAGLVREDQLTGALNRRGFEELFERE
ATRTQRSGQPLCVAMLDLDDFRRLNETHGHAGGDAALRHTVDVAKSVLRTTDAIARFGGEEFVLLLPDSTIFEASAAVIR
LQRALAQRSLLHEDVRVFVSFSGGVAMRRTDEAQDEVIRRADRAMYDSKAAGKNRVISAD
>Mature_539_residues
AEQPEHPNRPRGGLRRLLPWGGAGTARPATLPSVLGERDGAGLAAHQLVPAKAKSGAHDPAAATALLIRLFSHAAHPEAL
LLAFAEGIASLHGELGDMGVRLSTAYAAGDWPGYGRALRQLIDKYIRTIDTGDSLAEGRTEAEQLRDLLRHALGNALATL
LQRSPELAEEAQTLASALRHWRPGHELITLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQGQI
SVVRQLIAGPMDVGSIEQARGTLREVIYKQGLLKQGIADSKTAMREMMVSFVDRLDGMATSTGEYHDRVAGYSQAIGDAR
SIPDLNRLLQDVLQDTAGVQAQALAARDELLSARRQVEEAEHRIASLEQELKDVAGLVREDQLTGALNRRGFEELFEREA
TRTQRSGQPLCVAMLDLDDFRRLNETHGHAGGDAALRHTVDVAKSVLRTTDAIARFGGEEFVLLLPDSTIFEASAAVIRL
QRALAQRSLLHEDVRVFVSFSGGVAMRRTDEAQDEVIRRADRAMYDSKAAGKNRVISAD

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=223, Percent_Identity=30.9417040358744, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=33.5403726708075, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=33.7209302325581, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788381, Length=156, Percent_Identity=35.2564102564103, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787262, Length=183, Percent_Identity=29.5081967213115, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=32.0987654320988, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786584, Length=155, Percent_Identity=31.6129032258064, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788085, Length=186, Percent_Identity=29.0322580645161, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787541, Length=160, Percent_Identity=31.875, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87081974, Length=155, Percent_Identity=28.3870967741935, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787802, Length=181, Percent_Identity=25.414364640884, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 59023; Mature: 58891

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQPEHPNRPRGGLRRLLPWGGAGTARPATLPSVLGERDGAGLAAHQLVPAKAKSGAHD
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHCCHHHCCCCCC
PAAATALLIRLFSHAAHPEALLLAFAEGIASLHGELGDMGVRLSTAYAAGDWPGYGRALR
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHCCCCCHHHHHHH
QLIDKYIRTIDTGDSLAEGRTEAEQLRDLLRHALGNALATLLQRSPELAEEAQTLASALR
HHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
HWRPGHELITLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQGQ
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
ISVVRQLIAGPMDVGSIEQARGTLREVIYKQGLLKQGIADSKTAMREMMVSFVDRLDGMA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
TSTGEYHDRVAGYSQAIGDARSIPDLNRLLQDVLQDTAGVQAQALAARDELLSARRQVEE
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AEHRIASLEQELKDVAGLVREDQLTGALNRRGFEELFEREATRTQRSGQPLCVAMLDLDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEECCHHH
FRRLNETHGHAGGDAALRHTVDVAKSVLRTTDAIARFGGEEFVLLLPDSTIFEASAAVIR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHH
LQRALAQRSLLHEDVRVFVSFSGGVAMRRTDEAQDEVIRRADRAMYDSKAAGKNRVISAD
HHHHHHHHHHHHHHHHHHHEECCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AEQPEHPNRPRGGLRRLLPWGGAGTARPATLPSVLGERDGAGLAAHQLVPAKAKSGAHD
CCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHCCHHHCCCCCC
PAAATALLIRLFSHAAHPEALLLAFAEGIASLHGELGDMGVRLSTAYAAGDWPGYGRALR
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHCCCCCHHHHHHH
QLIDKYIRTIDTGDSLAEGRTEAEQLRDLLRHALGNALATLLQRSPELAEEAQTLASALR
HHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
HWRPGHELITLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQGQ
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
ISVVRQLIAGPMDVGSIEQARGTLREVIYKQGLLKQGIADSKTAMREMMVSFVDRLDGMA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
TSTGEYHDRVAGYSQAIGDARSIPDLNRLLQDVLQDTAGVQAQALAARDELLSARRQVEE
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AEHRIASLEQELKDVAGLVREDQLTGALNRRGFEELFEREATRTQRSGQPLCVAMLDLDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEECCHHH
FRRLNETHGHAGGDAALRHTVDVAKSVLRTTDAIARFGGEEFVLLLPDSTIFEASAAVIR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHH
LQRALAQRSLLHEDVRVFVSFSGGVAMRRTDEAQDEVIRRADRAMYDSKAAGKNRVISAD
HHHHHHHHHHHHHHHHHHHEECCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]