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The map label for this gene is amdA [H]

Identifier: 66045448

GI number: 66045448

Start: 2565581

End: 2567116

Strand: Direct

Name: amdA [H]

Synonym: Psyr_2208

Alternate gene names: 66045448

Gene position: 2565581-2567116 (Clockwise)

Preceding gene: 66045447

Following gene: 66045454

Centisome position: 42.1

GC content: 62.96

Gene sequence:

>1536_bases
ATGTCCGTAAAACGCCCCAGCAAAGCCGACTTTCTGATTGCCGCCCAGGACCATGGCTACGACCTGTCAGACAGTCAGAT
GGAGTCGTTCCTGAGTCTCGCAGACGAAACTCTGGGTTCCTACGAAGCCATCGATGCCTTATACGCTGCCCATGTCGCCC
AGCCGGCGCCGTTGCGCGAACACAGCAAACCGGCTGCGGCCGAGAACCTGTACGGGGCCTGGTACGTCAAGACCCACATT
GCCGGTGCTGCCACTGGCCCGCTGGCAGGCAGAACGGTGGTCATCAAGGACAACGTATCGGTGGCCGGTGTGCCCATGGC
CAATGGCTCGTTGAGCCTCGATGGTTACGTGCCGTCCGAAGACGCCACGGTGGTCAAGCGCCTGCTGGCCGCCGGTGCGA
CGGTGATTGGCAAGTCGGTGTGCGAAGACCTGTGCTTCTCCGGAGCCAGTTTTACCTCGGCCACCGGCGCGGTGAAAAAC
CCTTGGGACCTGACCCGCAACGCAGGTGGTTCATCCAGCGGCAGCGCCGTGCTGGTCGCCCTGCAGGAAGTGGACATGGC
CATCGGTGGCGATCAGGGTGGGTCGATCCGCATGCCGTCGGCCTGGAGCGGCATCGTCGGACACAAACCTACCTACAGCC
TGGTGCCTTACACCGGCGCCTTCCCGATTGAACGGACCATCGACCATGTCGGCCCGATGGCCAATAACGTGCGTGACTGC
GCGATTATGCTCGATGTGATCGCTGGCGCCGACGGGCTCGATTCAAGACAGAAAAACCCGCCAGCCGTCAGCTGCGTCGC
GACCCTCGATCAAGGCGTTGCAGGCCTGAAAATAGGCCTGCTGCGTGAAGGCTTCGCGATTCCCGGCATGTCCGAGCCGC
AGGTCGATGCACTGGTGCGCGCCGCCGTGGCGCAACTGGAAAGTCTCGGTGCGACGGTCGGTGACGCCAGTGCGCCGTGG
CACCGCGGAGTGGCGCTGGACATGTGGAATGTCATCGCCACCGATGGCGCGGCCTACCAGATGTTGCAAGGCAACGGTTA
TGGCATGAATGTCGACGGCTATTACGACCCCGAGATCATGAGTTACTTCGGCGCCAAGCGTCGCGAACACGCCGATGCGT
TATCCAGCAGCGTGCGTGCCGTGGCGCTGACCGGGCATTACAGCCTGAAAAACCTGCACGGTGCGTACTACGCCAAGGCG
CGGATGCTGGTGCCGGAACTCACCCGCCAATACGATGAAGCCTTCAAGCAATTCGACGTGCTGGTAATGCCGACCATGCC
GTTCGTGGCCACCACACTGACCGCCGCCGACGCGCCGATTGAAGAGTACGTGCACAGCGCGTTGAACATGCTGGCCAACA
CCGCACCGTTCGACCTGACCGGCCACCCGGCCACGTCGATACCGGCTGGTCTGGCCGAGGGACTGCCAGTCGCCATGATG
ATCGTCGCGCCGCGCTTCAAGGATGCCCTGGCGTTGCGTGTCGCGCAGGCTTATGAAACCGCCCGCGGAGCGTTTCCCAC
GCCACCAGGGGTTTGA

Upstream 100 bases:

>100_bases
TCCTCCGGATCGAGCCCAGGCCTGTGCGCGCCCTGCGCAGGCGGCTCCAGTCAATGACTGCCTGAACCCGGGCTGTCCTT
ACCGTTATCTGGAGAACACC

Downstream 100 bases:

>100_bases
GTCCGGAGACTGAACGAGGGGCATGCTCGCTCCATGCGAGCGGGTGTGCCCCTTCATCCCTCAAGGCTTCCAGACCTCCA
CATCCTGCGCATCCACCGCC

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MSVKRPSKADFLIAAQDHGYDLSDSQMESFLSLADETLGSYEAIDALYAAHVAQPAPLREHSKPAAAENLYGAWYVKTHI
AGAATGPLAGRTVVIKDNVSVAGVPMANGSLSLDGYVPSEDATVVKRLLAAGATVIGKSVCEDLCFSGASFTSATGAVKN
PWDLTRNAGGSSSGSAVLVALQEVDMAIGGDQGGSIRMPSAWSGIVGHKPTYSLVPYTGAFPIERTIDHVGPMANNVRDC
AIMLDVIAGADGLDSRQKNPPAVSCVATLDQGVAGLKIGLLREGFAIPGMSEPQVDALVRAAVAQLESLGATVGDASAPW
HRGVALDMWNVIATDGAAYQMLQGNGYGMNVDGYYDPEIMSYFGAKRREHADALSSSVRAVALTGHYSLKNLHGAYYAKA
RMLVPELTRQYDEAFKQFDVLVMPTMPFVATTLTAADAPIEEYVHSALNMLANTAPFDLTGHPATSIPAGLAEGLPVAMM
IVAPRFKDALALRVAQAYETARGAFPTPPGV

Sequences:

>Translated_511_residues
MSVKRPSKADFLIAAQDHGYDLSDSQMESFLSLADETLGSYEAIDALYAAHVAQPAPLREHSKPAAAENLYGAWYVKTHI
AGAATGPLAGRTVVIKDNVSVAGVPMANGSLSLDGYVPSEDATVVKRLLAAGATVIGKSVCEDLCFSGASFTSATGAVKN
PWDLTRNAGGSSSGSAVLVALQEVDMAIGGDQGGSIRMPSAWSGIVGHKPTYSLVPYTGAFPIERTIDHVGPMANNVRDC
AIMLDVIAGADGLDSRQKNPPAVSCVATLDQGVAGLKIGLLREGFAIPGMSEPQVDALVRAAVAQLESLGATVGDASAPW
HRGVALDMWNVIATDGAAYQMLQGNGYGMNVDGYYDPEIMSYFGAKRREHADALSSSVRAVALTGHYSLKNLHGAYYAKA
RMLVPELTRQYDEAFKQFDVLVMPTMPFVATTLTAADAPIEEYVHSALNMLANTAPFDLTGHPATSIPAGLAEGLPVAMM
IVAPRFKDALALRVAQAYETARGAFPTPPGV
>Mature_510_residues
SVKRPSKADFLIAAQDHGYDLSDSQMESFLSLADETLGSYEAIDALYAAHVAQPAPLREHSKPAAAENLYGAWYVKTHIA
GAATGPLAGRTVVIKDNVSVAGVPMANGSLSLDGYVPSEDATVVKRLLAAGATVIGKSVCEDLCFSGASFTSATGAVKNP
WDLTRNAGGSSSGSAVLVALQEVDMAIGGDQGGSIRMPSAWSGIVGHKPTYSLVPYTGAFPIERTIDHVGPMANNVRDCA
IMLDVIAGADGLDSRQKNPPAVSCVATLDQGVAGLKIGLLREGFAIPGMSEPQVDALVRAAVAQLESLGATVGDASAPWH
RGVALDMWNVIATDGAAYQMLQGNGYGMNVDGYYDPEIMSYFGAKRREHADALSSSVRAVALTGHYSLKNLHGAYYAKAR
MLVPELTRQYDEAFKQFDVLVMPTMPFVATTLTAADAPIEEYVHSALNMLANTAPFDLTGHPATSIPAGLAEGLPVAMMI
VAPRFKDALALRVAQAYETARGAFPTPPGV

Specific function: Hydrolyzes propionamides efficiently, and also at a lower efficiency, acetamide, acrylamide and indoleacetamide. This enzyme seems to be stereospecific and can lead to the production of a single enantiomer [H]

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=456, Percent_Identity=30.7017543859649, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI195972892, Length=450, Percent_Identity=27.3333333333333, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17543272, Length=423, Percent_Identity=28.8416075650118, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17537465, Length=440, Percent_Identity=24.7727272727273, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=260, Percent_Identity=26.5384615384615, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556264, Length=274, Percent_Identity=27.3722627737226, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71990152, Length=229, Percent_Identity=28.82096069869, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=439, Percent_Identity=25.2847380410023, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319685, Length=446, Percent_Identity=24.8878923766816, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648113, Length=438, Percent_Identity=31.0502283105023, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI161078093, Length=447, Percent_Identity=28.8590604026846, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24652985, Length=439, Percent_Identity=27.3348519362187, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922090, Length=439, Percent_Identity=27.3348519362187, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652981, Length=439, Percent_Identity=27.3348519362187, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652983, Length=439, Percent_Identity=27.3348519362187, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648441, Length=434, Percent_Identity=24.1935483870968, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24648439, Length=434, Percent_Identity=24.1935483870968, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24648435, Length=434, Percent_Identity=24.1935483870968, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24648437, Length=434, Percent_Identity=24.1935483870968, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI45550774, Length=434, Percent_Identity=24.1935483870968, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356731, Length=436, Percent_Identity=25.6880733944954, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24644968, Length=184, Percent_Identity=30.4347826086957, Blast_Score=96, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 53561; Mature: 53430

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKRPSKADFLIAAQDHGYDLSDSQMESFLSLADETLGSYEAIDALYAAHVAQPAPLRE
CCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHH
HSKPAAAENLYGAWYVKTHIAGAATGPLAGRTVVIKDNVSVAGVPMANGSLSLDGYVPSE
CCCCCHHHHCCEEEEEEEECCCCCCCCCCCCEEEEECCCEEEEEEECCCCEEECCCCCCC
DATVVKRLLAAGATVIGKSVCEDLCFSGASFTSATGAVKNPWDLTRNAGGSSSGSAVLVA
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCEEEEE
LQEVDMAIGGDQGGSIRMPSAWSGIVGHKPTYSLVPYTGAFPIERTIDHVGPMANNVRDC
EEHHHHHCCCCCCCCEECCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHCHHHCCHHHH
AIMLDVIAGADGLDSRQKNPPAVSCVATLDQGVAGLKIGLLREGFAIPGMSEPQVDALVR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEHHHHCCCCCCCCCCHHHHHHHH
AAVAQLESLGATVGDASAPWHRGVALDMWNVIATDGAAYQMLQGNGYGMNVDGYYDPEIM
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCHHHH
SYFGAKRREHADALSSSVRAVALTGHYSLKNLHGAYYAKARMLVPELTRQYDEAFKQFDV
HHHHHHHHHHHHHHHHCCEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
LVMPTMPFVATTLTAADAPIEEYVHSALNMLANTAPFDLTGHPATSIPAGLAEGLPVAMM
EEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEE
IVAPRFKDALALRVAQAYETARGAFPTPPGV
EECCCHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SVKRPSKADFLIAAQDHGYDLSDSQMESFLSLADETLGSYEAIDALYAAHVAQPAPLRE
CCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHH
HSKPAAAENLYGAWYVKTHIAGAATGPLAGRTVVIKDNVSVAGVPMANGSLSLDGYVPSE
CCCCCHHHHCCEEEEEEEECCCCCCCCCCCCEEEEECCCEEEEEEECCCCEEECCCCCCC
DATVVKRLLAAGATVIGKSVCEDLCFSGASFTSATGAVKNPWDLTRNAGGSSSGSAVLVA
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCEEEEE
LQEVDMAIGGDQGGSIRMPSAWSGIVGHKPTYSLVPYTGAFPIERTIDHVGPMANNVRDC
EEHHHHHCCCCCCCCEECCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHCHHHCCHHHH
AIMLDVIAGADGLDSRQKNPPAVSCVATLDQGVAGLKIGLLREGFAIPGMSEPQVDALVR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEHHHHCCCCCCCCCCHHHHHHHH
AAVAQLESLGATVGDASAPWHRGVALDMWNVIATDGAAYQMLQGNGYGMNVDGYYDPEIM
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCHHHH
SYFGAKRREHADALSSSVRAVALTGHYSLKNLHGAYYAKARMLVPELTRQYDEAFKQFDV
HHHHHHHHHHHHHHHHCCEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
LVMPTMPFVATTLTAADAPIEEYVHSALNMLANTAPFDLTGHPATSIPAGLAEGLPVAMM
EEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEE
IVAPRFKDALALRVAQAYETARGAFPTPPGV
EECCCHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2001397; 2254253 [H]