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The map label for this gene is prfB [H]

Identifier: 66044558

GI number: 66044558

Start: 1487230

End: 1488219

Strand: Direct

Name: prfB [H]

Synonym: Psyr_1310

Alternate gene names: 66044558

Gene position: 1487230-1488219 (Clockwise)

Preceding gene: 66044557

Following gene: 66044559

Centisome position: 24.41

GC content: 61.31

Gene sequence:

>990_bases
GTGAACCGCGAGCTCGAAGATCCAAACGTCTGGAACAACCCGGCCTACGCTCAGGAACTGGGGCGCGAGCGCTCCCTGCT
GGCGCAGATCGTCGAAACCCTGGACGAAATGTCCTCGGGTCTGGCCGATGCCAAAGACCTGTTGCTGATGTCTGCCGAAG
AAGAAGACCAGGCTGCCGTCGATGACGTCGCTGCCGAAGTCGAGCGCCTGCGCGAATCGCTGGAAAAGCTCGAATTCCGC
CGCATGTTCAGCGGCGACATGGACCCCAACAACGCCTACCTGGACATCCAGGCCGGCTCCGGCGGTACCGAAGCGCAGGA
CTGGGCGAACATCCTGCTGCGCATGTACCTGCGCTGGGCCGACAAGCGCGGTTTCGACGCCACCATCATGGAACTGTCCG
CCGGTGAAGTCGCCGGTATCAAGGGCGCTACCGTGCACATCAAGGGCGAGTACGCCTTCGGCTGGCTGCGTACCGAGATC
GGCGTTCATCGTCTGGTGCGCAAGAGCCCGTTCGACTCCGGCAACCGTCGCCACACCTCGTTCTCGGCCGTGTTCGTTTC
GCCGGAAATCGATGACAACATCGAAATCGACATCAACCCGGCGGACCTGCGCATCGACACCTACCGCTCCTCCGGTGCCG
GTGGTCAGCACGTAAACACCACTGACTCGGCGGTGCGTATCACCCACGTTCCGACCAACACCGTGGTCAGTTGCCAGAAC
GAGCGTTCCCAGCATGCGAACAAGGACACCGCCATGAAAATGCTGCGGGCCCGTTTGTATGAGCAGGAAGTGCAGAAGCG
CAACGCGGCGTCCCAGGCGCTCGAAGACACCAAGTCCGATATCGGCTGGGGTCACCAGATTCGCTCCTACGTACTGGACG
CCTCGCGTATCAAGGACCTGCGGACCAACATCGAACGCAGCGACTGCGACAAGGTGCTTGATGGCGATATCGACGAATAC
CTGATCGCGAGCCTCAAGCAAGGGCTGTAA

Upstream 100 bases:

>100_bases
ATCAACCCGATCCTAAACAGCATCAAGGATTTGTCCGAGCGCTCCGAAACCATTCGGGGGTATCTTTGACTACGATCACA
AACATGATCGTCTGACCGAA

Downstream 100 bases:

>100_bases
ACCGTCAGCCGACACCTCAAGTCCCTCGTCGCCAGGCGAGGGCGAACCTGTGATGGAATATTTAAAGACATGAGCGACCA
ACAACTCGACCCGCAAGCCC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNRELEDPNVWNNPAYAQELGRERSLLAQIVETLDEMSSGLADAKDLLLMSAEEEDQAAVDDVAAEVERLRESLEKLEFR
RMFSGDMDPNNAYLDIQAGSGGTEAQDWANILLRMYLRWADKRGFDATIMELSAGEVAGIKGATVHIKGEYAFGWLRTEI
GVHRLVRKSPFDSGNRRHTSFSAVFVSPEIDDNIEIDINPADLRIDTYRSSGAGGQHVNTTDSAVRITHVPTNTVVSCQN
ERSQHANKDTAMKMLRARLYEQEVQKRNAASQALEDTKSDIGWGHQIRSYVLDASRIKDLRTNIERSDCDKVLDGDIDEY
LIASLKQGL

Sequences:

>Translated_329_residues
MNRELEDPNVWNNPAYAQELGRERSLLAQIVETLDEMSSGLADAKDLLLMSAEEEDQAAVDDVAAEVERLRESLEKLEFR
RMFSGDMDPNNAYLDIQAGSGGTEAQDWANILLRMYLRWADKRGFDATIMELSAGEVAGIKGATVHIKGEYAFGWLRTEI
GVHRLVRKSPFDSGNRRHTSFSAVFVSPEIDDNIEIDINPADLRIDTYRSSGAGGQHVNTTDSAVRITHVPTNTVVSCQN
ERSQHANKDTAMKMLRARLYEQEVQKRNAASQALEDTKSDIGWGHQIRSYVLDASRIKDLRTNIERSDCDKVLDGDIDEY
LIASLKQGL
>Mature_329_residues
MNRELEDPNVWNNPAYAQELGRERSLLAQIVETLDEMSSGLADAKDLLLMSAEEEDQAAVDDVAAEVERLRESLEKLEFR
RMFSGDMDPNNAYLDIQAGSGGTEAQDWANILLRMYLRWADKRGFDATIMELSAGEVAGIKGATVHIKGEYAFGWLRTEI
GVHRLVRKSPFDSGNRRHTSFSAVFVSPEIDDNIEIDINPADLRIDTYRSSGAGGQHVNTTDSAVRITHVPTNTVVSCQN
ERSQHANKDTAMKMLRARLYEQEVQKRNAASQALEDTKSDIGWGHQIRSYVLDASRIKDLRTNIERSDCDKVLDGDIDEY
LIASLKQGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=310, Percent_Identity=35.8064516129032, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI34577120, Length=302, Percent_Identity=31.1258278145695, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI166795305, Length=210, Percent_Identity=34.2857142857143, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI2367172, Length=329, Percent_Identity=65.0455927051672, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1787462, Length=234, Percent_Identity=46.1538461538462, Blast_Score=197, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=299, Percent_Identity=27.7591973244147, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321295, Length=240, Percent_Identity=38.75, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19921226, Length=249, Percent_Identity=37.3493975903614, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 36863; Mature: 36863

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRELEDPNVWNNPAYAQELGRERSLLAQIVETLDEMSSGLADAKDLLLMSAEEEDQAAV
CCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCHHHHHH
DDVAAEVERLRESLEKLEFRRMFSGDMDPNNAYLDIQAGSGGTEAQDWANILLRMYLRWA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
DKRGFDATIMELSAGEVAGIKGATVHIKGEYAFGWLRTEIGVHRLVRKSPFDSGNRRHTS
CCCCCCCEEEEECCCCCCCCCCCEEEEECCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCE
FSAVFVSPEIDDNIEIDINPADLRIDTYRSSGAGGQHVNTTDSAVRITHVPTNTVVSCQN
EEEEEECCCCCCCEEEEECHHHEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEECCC
ERSQHANKDTAMKMLRARLYEQEVQKRNAASQALEDTKSDIGWGHQIRSYVLDASRIKDL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RTNIERSDCDKVLDGDIDEYLIASLKQGL
HHCCCHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNRELEDPNVWNNPAYAQELGRERSLLAQIVETLDEMSSGLADAKDLLLMSAEEEDQAAV
CCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCHHHHHH
DDVAAEVERLRESLEKLEFRRMFSGDMDPNNAYLDIQAGSGGTEAQDWANILLRMYLRWA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
DKRGFDATIMELSAGEVAGIKGATVHIKGEYAFGWLRTEIGVHRLVRKSPFDSGNRRHTS
CCCCCCCEEEEECCCCCCCCCCCEEEEECCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCE
FSAVFVSPEIDDNIEIDINPADLRIDTYRSSGAGGQHVNTTDSAVRITHVPTNTVVSCQN
EEEEEECCCCCCCEEEEECHHHEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEECCC
ERSQHANKDTAMKMLRARLYEQEVQKRNAASQALEDTKSDIGWGHQIRSYVLDASRIKDL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RTNIERSDCDKVLDGDIDEYLIASLKQGL
HHCCCHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA