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The map label for this gene is rnhA [H]

Identifier: 59712543

GI number: 59712543

Start: 2165741

End: 2166214

Strand: Direct

Name: rnhA [H]

Synonym: VF_1936

Alternate gene names: 59712543

Gene position: 2165741-2166214 (Clockwise)

Preceding gene: 172087736

Following gene: 172087738

Centisome position: 74.74

GC content: 41.77

Gene sequence:

>474_bases
ATGATAACAGAGATAATGAAACAGGTTGAAATTTTCACAGATGGTTCTTGTTTAGGTAACCCAGGTCCAGGCGGTTATGG
CATTGTAATGCGCTATAAAGGGACAGAAAAAACATTTTCAGAAGGGTTTAATAAAACCACTAACAACCGTATGGAAATGC
TAGCAGCAGTGGTAGCTCTACGTAAACTAAAGGAGCCATGCTCTGTCATATTAACAACAGACAGCCAGTATGTTCGTCAA
GGCATCACTCAATGGATCCACGGTTGGAAAAAAAGAGATTGGAAAAAAGCAGATAAAAAGCCAGTAGTAAATGCCGATCT
TTGGAAACAGTTAGATGCTGAATCTGAACGCCACAAAATTGATTGGCGCTGGGTAAAAGGTCACGCAGGTCATCGAGAGA
ACGAGATGTGTGATGAATTAGCAAGAACCGCAGCCGAAAACCCGACTCAAGATGATACGGGTTATCCTGGTTAA

Upstream 100 bases:

>100_bases
CATACCTGTGGTTTCTGTATCGAGAACTATAATTCGATTCGAAGAATTGTTGCTAGCATTCATAGGTTCATTTGTGTCAG
ACTATTTCTAAATTCCTCTA

Downstream 100 bases:

>100_bases
TCAGATTTCATTTTTCCTTGGCGGGTTTGAGCAACAGAAATAGGAGCCAACTTTGGCTTCTTTTTCCAAGCTGATTTTAT
TGGTCTTAATGGATACGTTC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MITEIMKQVEIFTDGSCLGNPGPGGYGIVMRYKGTEKTFSEGFNKTTNNRMEMLAAVVALRKLKEPCSVILTTDSQYVRQ
GITQWIHGWKKRDWKKADKKPVVNADLWKQLDAESERHKIDWRWVKGHAGHRENEMCDELARTAAENPTQDDTGYPG

Sequences:

>Translated_157_residues
MITEIMKQVEIFTDGSCLGNPGPGGYGIVMRYKGTEKTFSEGFNKTTNNRMEMLAAVVALRKLKEPCSVILTTDSQYVRQ
GITQWIHGWKKRDWKKADKKPVVNADLWKQLDAESERHKIDWRWVKGHAGHRENEMCDELARTAAENPTQDDTGYPG
>Mature_157_residues
MITEIMKQVEIFTDGSCLGNPGPGGYGIVMRYKGTEKTFSEGFNKTTNNRMEMLAAVVALRKLKEPCSVILTTDSQYVRQ
GITQWIHGWKKRDWKKADKKPVVNADLWKQLDAESERHKIDWRWVKGHAGHRENEMCDELARTAAENPTQDDTGYPG

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=143, Percent_Identity=33.5664335664336, Blast_Score=74, Evalue=7e-14,
Organism=Escherichia coli, GI1786408, Length=151, Percent_Identity=74.1721854304636, Blast_Score=246, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=34.0425531914894, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI17137476, Length=147, Percent_Identity=36.0544217687075, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45552509, Length=147, Percent_Identity=36.0544217687075, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 17915; Mature: 17915

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITEIMKQVEIFTDGSCLGNPGPGGYGIVMRYKGTEKTFSEGFNKTTNNRMEMLAAVVAL
CHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHH
RKLKEPCSVILTTDSQYVRQGITQWIHGWKKRDWKKADKKPVVNADLWKQLDAESERHKI
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC
DWRWVKGHAGHRENEMCDELARTAAENPTQDDTGYPG
CEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MITEIMKQVEIFTDGSCLGNPGPGGYGIVMRYKGTEKTFSEGFNKTTNNRMEMLAAVVAL
CHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHH
RKLKEPCSVILTTDSQYVRQGITQWIHGWKKRDWKKADKKPVVNADLWKQLDAESERHKI
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC
DWRWVKGHAGHRENEMCDELARTAAENPTQDDTGYPG
CEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA