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The map label for this gene is gloB

Identifier: 172087738

GI number: 172087738

Start: 2167002

End: 2167760

Strand: Direct

Name: gloB

Synonym: VF_1938

Alternate gene names: 172087738

Gene position: 2167002-2167760 (Clockwise)

Preceding gene: 59712543

Following gene: 59712546

Centisome position: 74.79

GC content: 39.26

Gene sequence:

>759_bases
ATGCTATCAGTAAAAAGCATACCTGCATTTAATGATAATTACATTTGGTTAATTCACAACAATGACAATCATTGTGTTGT
TGTCGATCCCGGTGATGCTACCCCTGTTTTAGAGTGCATTAAAGAGCATGACTTTATTTTAGATGCAATTTTAATTACAC
ACCACCACCATGATCATATCGGTGGCGTTCCTGAGTTGGTAAGACAATTCCCTAACGTCAATGTTGTAGGCCCAGAGAAT
GAACCTATTCCAACCTTAACTCACCCCGTTGGAGATGGCGATTTTGTTGAGTTATTTGATGAGCAATTCATGGTGTTAGG
TGTCCCTGGGCATACCAATGGTCATGTTGCTTATATTGGTGATGAAAAACTTTTCTGTGGTGATGCTCTTTTCTCTGCGG
GTTGCGGTCGATTATTCGAAGGCACTGCTGAACAAATGTTTAACTCATTACAAAAAATGGCAGCATTGCCTGATGAAACT
GAAGTCTACTGTGCACATGAATACACGGCATCAAACTTGGCATTTGCACTCGCCGTTGAACCAGATAATGATTATCTATT
ACGTTATAGAGAAAAAGTTCTTCATCTACGAGCTCATGGTAAATCGACGATTCCATCGACTTTACAACGAGAAAAACTCA
TTAATCCTTTTTTAAGAACAAGTGAAGCGAATGTAAAGAAATCGGTAGCAAGTAAAGTGCAAGATAGTACCGAGGTTGAG
ATCTTTACCGCATTAAGAAGATGGAAGGACGAGTTTTAA

Upstream 100 bases:

>100_bases
TCCCATGAATGTGGTTTTTCAATTTTTTTATCAAGCAGTGCTGGCTTAATCAAGATCGTTACCTATACCATCGAATAATA
AAAAGTTTGGAGAAATGTGA

Downstream 100 bases:

>100_bases
GTATGAAACTTGTTTGATGTGTGGTTGCAAAGTATTATTCGCCTCCAAATAAAAATATTAATACGATTAAACACACTGAA
GGCAATACATAATGAAACTG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLSVKSIPAFNDNYIWLIHNNDNHCVVVDPGDATPVLECIKEHDFILDAILITHHHHDHIGGVPELVRQFPNVNVVGPEN
EPIPTLTHPVGDGDFVELFDEQFMVLGVPGHTNGHVAYIGDEKLFCGDALFSAGCGRLFEGTAEQMFNSLQKMAALPDET
EVYCAHEYTASNLAFALAVEPDNDYLLRYREKVLHLRAHGKSTIPSTLQREKLINPFLRTSEANVKKSVASKVQDSTEVE
IFTALRRWKDEF

Sequences:

>Translated_252_residues
MLSVKSIPAFNDNYIWLIHNNDNHCVVVDPGDATPVLECIKEHDFILDAILITHHHHDHIGGVPELVRQFPNVNVVGPEN
EPIPTLTHPVGDGDFVELFDEQFMVLGVPGHTNGHVAYIGDEKLFCGDALFSAGCGRLFEGTAEQMFNSLQKMAALPDET
EVYCAHEYTASNLAFALAVEPDNDYLLRYREKVLHLRAHGKSTIPSTLQREKLINPFLRTSEANVKKSVASKVQDSTEVE
IFTALRRWKDEF
>Mature_252_residues
MLSVKSIPAFNDNYIWLIHNNDNHCVVVDPGDATPVLECIKEHDFILDAILITHHHHDHIGGVPELVRQFPNVNVVGPEN
EPIPTLTHPVGDGDFVELFDEQFMVLGVPGHTNGHVAYIGDEKLFCGDALFSAGCGRLFEGTAEQMFNSLQKMAALPDET
EVYCAHEYTASNLAFALAVEPDNDYLLRYREKVLHLRAHGKSTIPSTLQREKLINPFLRTSEANVKKSVASKVQDSTEVE
IFTALRRWKDEF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI116642887, Length=262, Percent_Identity=38.9312977099237, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI21703352, Length=262, Percent_Identity=38.9312977099237, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI14150041, Length=243, Percent_Identity=40.7407407407407, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI94538322, Length=263, Percent_Identity=36.5019011406844, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI94538320, Length=263, Percent_Identity=36.5019011406844, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI46361987, Length=193, Percent_Identity=41.9689119170984, Blast_Score=140, Evalue=8e-34,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=47.0119521912351, Blast_Score=227, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17536925, Length=265, Percent_Identity=35.8490566037736, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17538952, Length=158, Percent_Identity=32.9113924050633, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320478, Length=260, Percent_Identity=28.4615384615385, Blast_Score=95, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6324614, Length=261, Percent_Identity=27.5862068965517, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356335, Length=260, Percent_Identity=37.6923076923077, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24667703, Length=261, Percent_Identity=37.9310344827586, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24667711, Length=260, Percent_Identity=37.6923076923077, Blast_Score=174, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_VIBF1 (Q5E3G3)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205321.2
- HSSP:   Q9SID3
- ProteinModelPortal:   Q5E3G3
- SMR:   Q5E3G3
- STRING:   Q5E3G3
- GeneID:   3279201
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1938
- NMPDR:   fig|312309.3.peg.1938
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK874727
- BioCyc:   VFIS312309:VF1938-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28246; Mature: 28246

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVKSIPAFNDNYIWLIHNNDNHCVVVDPGDATPVLECIKEHDFILDAILITHHHHDHI
CCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCC
GGVPELVRQFPNVNVVGPENEPIPTLTHPVGDGDFVELFDEQFMVLGVPGHTNGHVAYIG
CCHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCEEEEEC
DEKLFCGDALFSAGCGRLFEGTAEQMFNSLQKMAALPDETEVYCAHEYTASNLAFALAVE
CCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEC
PDNDYLLRYREKVLHLRAHGKSTIPSTLQREKLINPFLRTSEANVKKSVASKVQDSTEVE
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHH
IFTALRRWKDEF
HHHHHHHHHCCC
>Mature Secondary Structure
MLSVKSIPAFNDNYIWLIHNNDNHCVVVDPGDATPVLECIKEHDFILDAILITHHHHDHI
CCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCC
GGVPELVRQFPNVNVVGPENEPIPTLTHPVGDGDFVELFDEQFMVLGVPGHTNGHVAYIG
CCHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCEEEEEC
DEKLFCGDALFSAGCGRLFEGTAEQMFNSLQKMAALPDETEVYCAHEYTASNLAFALAVE
CCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEC
PDNDYLLRYREKVLHLRAHGKSTIPSTLQREKLINPFLRTSEANVKKSVASKVQDSTEVE
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHH
IFTALRRWKDEF
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA