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The map label for this gene is uraA [H]

Identifier: 59712533

GI number: 59712533

Start: 2154976

End: 2156196

Strand: Direct

Name: uraA [H]

Synonym: VF_1926

Alternate gene names: 59712533

Gene position: 2154976-2156196 (Clockwise)

Preceding gene: 59712531

Following gene: 59712535

Centisome position: 74.37

GC content: 42.26

Gene sequence:

>1221_bases
ATGCTATTTGTTGCATTTGGTGCACTTGTACTTGTCCCCCTCCTTACTGGCCTAGATCCCAATGTTGCTCTGTTTGGTGC
TGGTGTCGGTACCCTACTCTTTCAATTAGTTACACGTCGTTCTGTTCCTATCTTCCTCGCTTCTTCCTTTGCTTTTATCG
CTCCAATTATCTACGGAACCCAAACATGGGGGATCCCTGCTACAATGGGTGGCCTAATGGTTGCAGGTTTAGTTTATGTC
GCTTTGGGCGCTGTAATTAAAGTAAGAGGTGTGGCTTTTATCCATAAGTTACTGCCACCAGTTGTTGTTGGCCCAGTTAT
TATGGTAATTGGTTTAGGATTAGCACCAACAGCTGTAAATATGGCACTAGGTAAAACAGGCGATGGTGCTTATCAACTTG
TTGATGGTCAATTAGCGATGTGGATTGCTGCGGCTTCATTATTTACTACGATTGCCGTTAGTGTTTTTGCAAAAGGTTTC
TTTAAGCTTCTTCCAATTATTTCAGGCATCATAGTTGGCTACTCTCTTAGCCTTATCTTTGGTGTTGTTGATTTTACCCC
AGTTCATCAAGCCGCTTGGTTTGCACTACCAAACTTCACTTTCCCTGAGTTCAACATTAATGCGATTCTGTTTATGATCC
CAGTTGCAATTGCACCTGCTGTTGAACACGTTGGTGACATGCTTGCAATTTCAAACGTGACAGGGAAAGATTACCTTAAA
AAACCAGGGTTACACCGCACTATCACAGGCGACGGTATTGCAACAATGGCCGCTTCTTTCATTGGTGCGCCACCAAATAC
AACATACAGTGAAGTGACCGGCGCCGTAATGCTGACTAAAGCTTTTAACCCTGTAATCATGACATGGGCAGCTGTGTGCG
CCATTGTTCTTGCGTTAGTTGGTAAATTAGGTGCAATGCTACAAACCATTCCAGTTCCTGTGATGGGTGGAATTATGATC
TTATTATTTGGTTCTATTGCAACAGTAGGTCTAAATACGTTGATTAAAAACCAAGTTGACTTGCATAAGGCTCGTAATCT
TACGATTGTTGGTGTAACGCTTGTTTTCGGTATTGGTGGTATGGCAGTAGGTATTGGTGACTTCAATCTTCAAGGTGTAA
GTCTTTGCGGTATCGTTGCTATTACTCTGAACTTAATTCTTCCAAATGACTTAGGTGAAAGCCACGTTGTTGATAATGCA
CAAATGGAAGAAGATAAATAG

Upstream 100 bases:

>100_bases
ACGACCGAAATCAAGATATTTTTATTTCTAATAATGATAGAATCCCTCAAATTCACTACTGGAAATGGAAATGCTTTATA
ATTTATTTCAGGGATTTCAA

Downstream 100 bases:

>100_bases
ATTAAGGGGTTAGGGTCTAGATAATAAAAAGGCTTGATGATTAATTCATCAAGCCTTTTTTTAATTCTTCATTTCTAGTT
TCTAAACTAAATTATTTAGT

Product: uracil transporter

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MLFVAFGALVLVPLLTGLDPNVALFGAGVGTLLFQLVTRRSVPIFLASSFAFIAPIIYGTQTWGIPATMGGLMVAGLVYV
ALGAVIKVRGVAFIHKLLPPVVVGPVIMVIGLGLAPTAVNMALGKTGDGAYQLVDGQLAMWIAAASLFTTIAVSVFAKGF
FKLLPIISGIIVGYSLSLIFGVVDFTPVHQAAWFALPNFTFPEFNINAILFMIPVAIAPAVEHVGDMLAISNVTGKDYLK
KPGLHRTITGDGIATMAASFIGAPPNTTYSEVTGAVMLTKAFNPVIMTWAAVCAIVLALVGKLGAMLQTIPVPVMGGIMI
LLFGSIATVGLNTLIKNQVDLHKARNLTIVGVTLVFGIGGMAVGIGDFNLQGVSLCGIVAITLNLILPNDLGESHVVDNA
QMEEDK

Sequences:

>Translated_406_residues
MLFVAFGALVLVPLLTGLDPNVALFGAGVGTLLFQLVTRRSVPIFLASSFAFIAPIIYGTQTWGIPATMGGLMVAGLVYV
ALGAVIKVRGVAFIHKLLPPVVVGPVIMVIGLGLAPTAVNMALGKTGDGAYQLVDGQLAMWIAAASLFTTIAVSVFAKGF
FKLLPIISGIIVGYSLSLIFGVVDFTPVHQAAWFALPNFTFPEFNINAILFMIPVAIAPAVEHVGDMLAISNVTGKDYLK
KPGLHRTITGDGIATMAASFIGAPPNTTYSEVTGAVMLTKAFNPVIMTWAAVCAIVLALVGKLGAMLQTIPVPVMGGIMI
LLFGSIATVGLNTLIKNQVDLHKARNLTIVGVTLVFGIGGMAVGIGDFNLQGVSLCGIVAITLNLILPNDLGESHVVDNA
QMEEDK
>Mature_406_residues
MLFVAFGALVLVPLLTGLDPNVALFGAGVGTLLFQLVTRRSVPIFLASSFAFIAPIIYGTQTWGIPATMGGLMVAGLVYV
ALGAVIKVRGVAFIHKLLPPVVVGPVIMVIGLGLAPTAVNMALGKTGDGAYQLVDGQLAMWIAAASLFTTIAVSVFAKGF
FKLLPIISGIIVGYSLSLIFGVVDFTPVHQAAWFALPNFTFPEFNINAILFMIPVAIAPAVEHVGDMLAISNVTGKDYLK
KPGLHRTITGDGIATMAASFIGAPPNTTYSEVTGAVMLTKAFNPVIMTWAAVCAIVLALVGKLGAMLQTIPVPVMGGIMI
LLFGSIATVGLNTLIKNQVDLHKARNLTIVGVTLVFGIGGMAVGIGDFNLQGVSLCGIVAITLNLILPNDLGESHVVDNA
QMEEDK

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680145, Length=387, Percent_Identity=24.031007751938, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI44680143, Length=390, Percent_Identity=24.3589743589744, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI44680148, Length=404, Percent_Identity=22.7722772277228, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI40316845, Length=404, Percent_Identity=22.7722772277228, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1788843, Length=402, Percent_Identity=39.3034825870647, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI87081818, Length=402, Percent_Identity=38.5572139303483, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI87082181, Length=402, Percent_Identity=25.6218905472637, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87082178, Length=341, Percent_Identity=25.8064516129032, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790087, Length=373, Percent_Identity=24.3967828418231, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558856, Length=177, Percent_Identity=28.2485875706215, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17542262, Length=173, Percent_Identity=27.7456647398844, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17542260, Length=229, Percent_Identity=24.0174672489083, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21356175, Length=315, Percent_Identity=28.2539682539683, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 42283; Mature: 42283

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFVAFGALVLVPLLTGLDPNVALFGAGVGTLLFQLVTRRSVPIFLASSFAFIAPIIYGT
CEEHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCC
QTWGIPATMGGLMVAGLVYVALGAVIKVRGVAFIHKLLPPVVVGPVIMVIGLGLAPTAVN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MALGKTGDGAYQLVDGQLAMWIAAASLFTTIAVSVFAKGFFKLLPIISGIIVGYSLSLIF
HHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVVDFTPVHQAAWFALPNFTFPEFNINAILFMIPVAIAPAVEHVGDMLAISNVTGKDYLK
HHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
KPGLHRTITGDGIATMAASFIGAPPNTTYSEVTGAVMLTKAFNPVIMTWAAVCAIVLALV
CCCCCEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GKLGAMLQTIPVPVMGGIMILLFGSIATVGLNTLIKNQVDLHKARNLTIVGVTLVFGIGG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHCCC
MAVGIGDFNLQGVSLCGIVAITLNLILPNDLGESHVVDNAQMEEDK
CEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLFVAFGALVLVPLLTGLDPNVALFGAGVGTLLFQLVTRRSVPIFLASSFAFIAPIIYGT
CEEHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCC
QTWGIPATMGGLMVAGLVYVALGAVIKVRGVAFIHKLLPPVVVGPVIMVIGLGLAPTAVN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MALGKTGDGAYQLVDGQLAMWIAAASLFTTIAVSVFAKGFFKLLPIISGIIVGYSLSLIF
HHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVVDFTPVHQAAWFALPNFTFPEFNINAILFMIPVAIAPAVEHVGDMLAISNVTGKDYLK
HHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
KPGLHRTITGDGIATMAASFIGAPPNTTYSEVTGAVMLTKAFNPVIMTWAAVCAIVLALV
CCCCCEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GKLGAMLQTIPVPVMGGIMILLFGSIATVGLNTLIKNQVDLHKARNLTIVGVTLVFGIGG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHCCC
MAVGIGDFNLQGVSLCGIVAITLNLILPNDLGESHVVDNAQMEEDK
CEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]