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The map label for this gene is rlmG [H]

Identifier: 59711330

GI number: 59711330

Start: 789785

End: 790933

Strand: Reverse

Name: rlmG [H]

Synonym: VF_0723

Alternate gene names: 59711330

Gene position: 790933-789785 (Counterclockwise)

Preceding gene: 59711343

Following gene: 59711329

Centisome position: 27.3

GC content: 36.99

Gene sequence:

>1149_bases
ATGAAAACAGAACTGAGTCTACTTGACCGAAGCCTAAACTTACAACGTTACCCTAAACGTGCTCAAGAACTACTGCAAGC
ATGGGATGCAGGTGATGAATACATCATTAAATATGTAGAAGAAGAGCTAAATCTAGAAGATGGCAAAAACATCCTTATCT
TAAATGATAATTTTGGTGCATTATCATGCTGGTTCTCAGACAAGCATAACGTAACAATGATGACCGATTCGTTTGTTTCA
CAACGTGGTACATTAAAAAACCTACAACGAAACCAATGTAACCGTGTACAGCTTATTACATCAACTGAAGAGATGCCACA
AGGTTTCGATTTAGTATTAATGCAGATCCCAAAAAACAATCGAATGCTCACATGGCAACTACAACAACTGCGTCAATCAA
TGGACAGTAGCTGCCCTATTATCGCTGTAAATAAAGCAAAAGAAATCCATAGTTCAACACTTGAGTTATTTGAAGACTAC
CTTGGGGAAACTAAAACATCGCTTGCTTGGAAGAAGCACCGTTTAGTGTTTTCAAATGCAAACGTATCGAATCCAAAAAC
CATTGCTGAAGCAGTATGTTGGAGTGTAGATAATGAAGACATCGATCTTCTTAATTACCCTAACGTATATTCTGGAGAAA
AATTAGACCAAGGTGCTCGCTTTATGCTTGACCATATTCCTTCAGATCCTGAATTACGACACATTATAGATCTTGGTTGT
GGCAATGGAGTACTCAGTGTAAAAGCTGGTCAATTAAACCCTGAAGCTCGTATTACTTGTGTTGATGAAAGCTTTATGGC
TGTAGAGTCAGCACACCGCAATTTAGAAGTTAACTTAGGTAAAGAACGTCAATTTCAATTTATTGCTAATAATTGTCTCG
ATGGTTTTAAAAAGCACAGTAGTTACCTTGTACTTTGCAACCCTCCATTCCATCAAGGTCAGGCAATTACCGATCATATT
GCATGGCAAATGTTCTGTGATGCTAAGCACATCTTGTGCAAAGATGGTAAATTATTAGTTATTGGAAACCGTCATCTTGA
TTATGATGGTAAATTATGTCGCTTATTTGGTGAAGAAAATGTCACCACAGTTGCTAGCAATTCAAAATTCGTTATTTTAG
AAGCTGTTAAAGCCGAAAAATCAAAATAA

Upstream 100 bases:

>100_bases
ATCATATAATTCCTTTCTTTATTTGAATTCTTCTGTTTCCACTTTATACACCATTTGCGACAATAGCTAGAAGCCTTCCC
TATTTGAAGATCAAATCATT

Downstream 100 bases:

>100_bases
AAAAGGATTACACCATGAAAAAGGTCATCATTGCCCTTTCTGCACTTTTTTTAGCTGCTTGTAGTGCTCCATCAGATCCA
CAATTGGATATTCAACCTAA

Product: methyltransferase small subunit

Products: NA

Alternate protein names: 23S rRNA m2G1835 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmG [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKTELSLLDRSLNLQRYPKRAQELLQAWDAGDEYIIKYVEEELNLEDGKNILILNDNFGALSCWFSDKHNVTMMTDSFVS
QRGTLKNLQRNQCNRVQLITSTEEMPQGFDLVLMQIPKNNRMLTWQLQQLRQSMDSSCPIIAVNKAKEIHSSTLELFEDY
LGETKTSLAWKKHRLVFSNANVSNPKTIAEAVCWSVDNEDIDLLNYPNVYSGEKLDQGARFMLDHIPSDPELRHIIDLGC
GNGVLSVKAGQLNPEARITCVDESFMAVESAHRNLEVNLGKERQFQFIANNCLDGFKKHSSYLVLCNPPFHQGQAITDHI
AWQMFCDAKHILCKDGKLLVIGNRHLDYDGKLCRLFGEENVTTVASNSKFVILEAVKAEKSK

Sequences:

>Translated_382_residues
MKTELSLLDRSLNLQRYPKRAQELLQAWDAGDEYIIKYVEEELNLEDGKNILILNDNFGALSCWFSDKHNVTMMTDSFVS
QRGTLKNLQRNQCNRVQLITSTEEMPQGFDLVLMQIPKNNRMLTWQLQQLRQSMDSSCPIIAVNKAKEIHSSTLELFEDY
LGETKTSLAWKKHRLVFSNANVSNPKTIAEAVCWSVDNEDIDLLNYPNVYSGEKLDQGARFMLDHIPSDPELRHIIDLGC
GNGVLSVKAGQLNPEARITCVDESFMAVESAHRNLEVNLGKERQFQFIANNCLDGFKKHSSYLVLCNPPFHQGQAITDHI
AWQMFCDAKHILCKDGKLLVIGNRHLDYDGKLCRLFGEENVTTVASNSKFVILEAVKAEKSK
>Mature_382_residues
MKTELSLLDRSLNLQRYPKRAQELLQAWDAGDEYIIKYVEEELNLEDGKNILILNDNFGALSCWFSDKHNVTMMTDSFVS
QRGTLKNLQRNQCNRVQLITSTEEMPQGFDLVLMQIPKNNRMLTWQLQQLRQSMDSSCPIIAVNKAKEIHSSTLELFEDY
LGETKTSLAWKKHRLVFSNANVSNPKTIAEAVCWSVDNEDIDLLNYPNVYSGEKLDQGARFMLDHIPSDPELRHIIDLGC
GNGVLSVKAGQLNPEARITCVDESFMAVESAHRNLEVNLGKERQFQFIANNCLDGFKKHSSYLVLCNPPFHQGQAITDHI
AWQMFCDAKHILCKDGKLLVIGNRHLDYDGKLCRLFGEENVTTVASNSKFVILEAVKAEKSK

Specific function: Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA [H]

COG id: COG2813

COG function: function code J; 16S RNA G1207 methylase RsmC

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmG family [H]

Homologues:

Organism=Escherichia coli, GI87082206, Length=377, Percent_Identity=42.9708222811671, Blast_Score=292, Evalue=2e-80,
Organism=Escherichia coli, GI1790830, Length=290, Percent_Identity=26.8965517241379, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017237
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: =2.1.1.174 [H]

Molecular weight: Translated: 43458; Mature: 43458

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTELSLLDRSLNLQRYPKRAQELLQAWDAGDEYIIKYVEEELNLEDGKNILILNDNFGA
CCCHHHHHHHHCCHHHCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCE
LSCWFSDKHNVTMMTDSFVSQRGTLKNLQRNQCNRVQLITSTEEMPQGFDLVLMQIPKNN
EEEEECCCCCEEEEEHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCC
RMLTWQLQQLRQSMDSSCPIIAVNKAKEIHSSTLELFEDYLGETKTSLAWKKHRLVFSNA
CEEEHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEECC
NVSNPKTIAEAVCWSVDNEDIDLLNYPNVYSGEKLDQGARFMLDHIPSDPELRHIIDLGC
CCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCHHHEEEECC
GNGVLSVKAGQLNPEARITCVDESFMAVESAHRNLEVNLGKERQFQFIANNCLDGFKKHS
CCCEEEEECCCCCCCCEEEEECHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCC
SYLVLCNPPFHQGQAITDHIAWQMFCDAKHILCKDGKLLVIGNRHLDYDGKLCRLFGEEN
CEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECCEECCCCCEEEEECCCC
VTTVASNSKFVILEAVKAEKSK
EEEEECCCCEEEEEEECCCCCC
>Mature Secondary Structure
MKTELSLLDRSLNLQRYPKRAQELLQAWDAGDEYIIKYVEEELNLEDGKNILILNDNFGA
CCCHHHHHHHHCCHHHCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCE
LSCWFSDKHNVTMMTDSFVSQRGTLKNLQRNQCNRVQLITSTEEMPQGFDLVLMQIPKNN
EEEEECCCCCEEEEEHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCC
RMLTWQLQQLRQSMDSSCPIIAVNKAKEIHSSTLELFEDYLGETKTSLAWKKHRLVFSNA
CEEEHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEECC
NVSNPKTIAEAVCWSVDNEDIDLLNYPNVYSGEKLDQGARFMLDHIPSDPELRHIIDLGC
CCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCHHHEEEECC
GNGVLSVKAGQLNPEARITCVDESFMAVESAHRNLEVNLGKERQFQFIANNCLDGFKKHS
CCCEEEEECCCCCCCCEEEEECHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCC
SYLVLCNPPFHQGQAITDHIAWQMFCDAKHILCKDGKLLVIGNRHLDYDGKLCRLFGEEN
CEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECCEECCCCCEEEEECCCC
VTTVASNSKFVILEAVKAEKSK
EEEEECCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA