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The map label for this gene is lepA [H]

Identifier: 55980710

GI number: 55980710

Start: 707097

End: 708929

Strand: Reverse

Name: lepA [H]

Synonym: TTHA0741

Alternate gene names: 55980710

Gene position: 708929-707097 (Counterclockwise)

Preceding gene: 55980711

Following gene: 55980709

Centisome position: 38.33

GC content: 67.16

Gene sequence:

>1833_bases
ATGGTGAGGATGGACCTAAGCCGCATCCGCAACTTCTCCATCATCGCCCACGTGGACCACGGCAAGTCCACCCTGGCCGA
CCGCATCCTGGAGCTCACCCACGCGGTGAGCGACCGGGAGATGCGGGAGCAGTTCTTGGACTCCCTGGAGCTGGAGAGGG
AGCGGGGCATCACCATCAAGGCGAGCGCCGTCAGGGTAACCTACCGGGCCAAGGACGGGGAGGAGTACGTCTTCCACCTC
ATTGACACCCCGGGGCACGTGGACTTCACCTACGAGGTCTCCCGGGCCCTGGCGGCGGTGGAGGGGGTGCTCCTCGTGGT
GGACGCGAGCCAGGGGGTGGAGGCGGAGACCCTGGCCAAGTTCTACATGGCCTTGGAGCACGGGCACGTGATCATCCCGG
TGATCAACAAAATAGACCTCCCCAACGCCCGCCCCCTGGAGGTGGCCCTCGAGGTGGAGGAGGTCCTGGGCCTTCCCGCC
GACGAGGCCATCTTCGCCTCGGGGAAGACGGGGGAGGGCGTGGAGGAGATCCTCGAGGCCATCGTCCAGCGCATCCCGCC
CCCCAAAGGGGACCCCGAGGCCCCCCTGAAGGCCCTCATCTTTGACTCCGTCTACGACGCCTACCAGGGGGTGATCCCCT
ACCTCCGCCTCTTTGAGGGGAGGGTGCGCCCCGGGGACCGGATCCGCATCTACTCCACGGGGAAGGAGTTCACCGTGGAC
AAGGTGGGGGTCTTCACCCCCCAGGGCCTGGTGGCCACGGAAGCGCTCGAGGCCGGGGAGGTGGGGTGGCTCGTGGCCGC
CATCCGCGACATCCACGACGTCCAGGTGGGGGACACCATCACCCTGGCGGACCGCCCCACCCCCTCCCCCTACCCCGGCT
TCCGCCCGGCCAAGCCCGTGGTCTTCGCCGGGCTTTACCCCGTGGACTCCGGGGACTACGGGAAGCTCCGCGACGCCCTG
GAGAAGCTGAAGCTCAACGACGCCGCCCTCACCTTTGAGCCCGAGTCCTCCACCGCCTTGGGCTTCGGCTTCCGCTGCGG
CTTCCTCGGGCTTCTCCACGCCGAGATCGTCCAGGAGAGGCTGGAGCGGGAGTTCGGCCTTTCCCTCATCGCCACCGCCC
CGAGCGTGGTCTACAAGGTGCGGCTCAAAAGCGGGGAGGAGGTGGAGGTCCACAACCCCGCCGACCTCCCCGACCCCACG
CGGATTGAGGAGATCCTCGAGCCCTACGTGAAGCTCACCATCTTCACCCCCGAGGAGTACGTGGGAAGCCTCATGCAGCT
CCTCCAGGAGAAGCGGGGCCGCCTCGTCAACATGAACTACCTCCCCGGGGCCCAGAAGCGGGTGGAGCTCGTCTACGAGG
CCCCCTTCGCCGAGATCCTCTACGACTTCCACGACCGGCTGAAAAGCGTCTCCCGCGGGTACGCCTCCATGGACTACGAG
CAGGCGGGCTACCGGCCCGGGGACCTGGTGAAGGTGAACGTCCTGGTGCACGGGGAGGTGGTGGACGCCCTCACCTTCAT
CGCCCACCGGGAGAAGGCCTACACCATGGCCCGGGCCATCGTGGACAAGCTCGCCGAGGTCATCCCCAGGCAGCTCTTTG
AGGTGCCCATCCAGGCGGCCATCGGGGGCAAGATCATCGCCCGGGCCACGGTGAAGGCCCTGAGGAAGGACGTCCTCGCC
AAGTGCTACGGCGGGGACGTGACCCGGAAGAAGAAGCTTTTGGAGAAGCAGAAGGAGGGCAAGAAGCGCCTCAAGGCCAT
CGGCAAGGTGGAGGTTCCCCAGGAGGCCTTCCTGGCGGTGCTCTCGGCGGGTCGCGATGAGCCTAAGGGTTAG

Upstream 100 bases:

>100_bases
TTTCGCGAGGTCTGGCGGTACAAGGGGTGCCGGCTCCTGGAGCGGCGCCCCTCCTAGGGACCCGAGTGCGCCCTTGACTT
TGCCCGAGGGAGCCCGGAGG

Downstream 100 bases:

>100_bases
GCTCGCCCTGGTCATCGCCCTCCTCGCCTTCCTGCCCAACCTGGTCCTGGCCCTCACCCTGGGGGCCCTGGGGGAAGGCC
CCTGGCCTCCCCTCCTCCTC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MVRMDLSRIRNFSIIAHVDHGKSTLADRILELTHAVSDREMREQFLDSLELERERGITIKASAVRVTYRAKDGEEYVFHL
IDTPGHVDFTYEVSRALAAVEGVLLVVDASQGVEAETLAKFYMALEHGHVIIPVINKIDLPNARPLEVALEVEEVLGLPA
DEAIFASGKTGEGVEEILEAIVQRIPPPKGDPEAPLKALIFDSVYDAYQGVIPYLRLFEGRVRPGDRIRIYSTGKEFTVD
KVGVFTPQGLVATEALEAGEVGWLVAAIRDIHDVQVGDTITLADRPTPSPYPGFRPAKPVVFAGLYPVDSGDYGKLRDAL
EKLKLNDAALTFEPESSTALGFGFRCGFLGLLHAEIVQERLEREFGLSLIATAPSVVYKVRLKSGEEVEVHNPADLPDPT
RIEEILEPYVKLTIFTPEEYVGSLMQLLQEKRGRLVNMNYLPGAQKRVELVYEAPFAEILYDFHDRLKSVSRGYASMDYE
QAGYRPGDLVKVNVLVHGEVVDALTFIAHREKAYTMARAIVDKLAEVIPRQLFEVPIQAAIGGKIIARATVKALRKDVLA
KCYGGDVTRKKKLLEKQKEGKKRLKAIGKVEVPQEAFLAVLSAGRDEPKG

Sequences:

>Translated_610_residues
MVRMDLSRIRNFSIIAHVDHGKSTLADRILELTHAVSDREMREQFLDSLELERERGITIKASAVRVTYRAKDGEEYVFHL
IDTPGHVDFTYEVSRALAAVEGVLLVVDASQGVEAETLAKFYMALEHGHVIIPVINKIDLPNARPLEVALEVEEVLGLPA
DEAIFASGKTGEGVEEILEAIVQRIPPPKGDPEAPLKALIFDSVYDAYQGVIPYLRLFEGRVRPGDRIRIYSTGKEFTVD
KVGVFTPQGLVATEALEAGEVGWLVAAIRDIHDVQVGDTITLADRPTPSPYPGFRPAKPVVFAGLYPVDSGDYGKLRDAL
EKLKLNDAALTFEPESSTALGFGFRCGFLGLLHAEIVQERLEREFGLSLIATAPSVVYKVRLKSGEEVEVHNPADLPDPT
RIEEILEPYVKLTIFTPEEYVGSLMQLLQEKRGRLVNMNYLPGAQKRVELVYEAPFAEILYDFHDRLKSVSRGYASMDYE
QAGYRPGDLVKVNVLVHGEVVDALTFIAHREKAYTMARAIVDKLAEVIPRQLFEVPIQAAIGGKIIARATVKALRKDVLA
KCYGGDVTRKKKLLEKQKEGKKRLKAIGKVEVPQEAFLAVLSAGRDEPKG
>Mature_610_residues
MVRMDLSRIRNFSIIAHVDHGKSTLADRILELTHAVSDREMREQFLDSLELERERGITIKASAVRVTYRAKDGEEYVFHL
IDTPGHVDFTYEVSRALAAVEGVLLVVDASQGVEAETLAKFYMALEHGHVIIPVINKIDLPNARPLEVALEVEEVLGLPA
DEAIFASGKTGEGVEEILEAIVQRIPPPKGDPEAPLKALIFDSVYDAYQGVIPYLRLFEGRVRPGDRIRIYSTGKEFTVD
KVGVFTPQGLVATEALEAGEVGWLVAAIRDIHDVQVGDTITLADRPTPSPYPGFRPAKPVVFAGLYPVDSGDYGKLRDAL
EKLKLNDAALTFEPESSTALGFGFRCGFLGLLHAEIVQERLEREFGLSLIATAPSVVYKVRLKSGEEVEVHNPADLPDPT
RIEEILEPYVKLTIFTPEEYVGSLMQLLQEKRGRLVNMNYLPGAQKRVELVYEAPFAEILYDFHDRLKSVSRGYASMDYE
QAGYRPGDLVKVNVLVHGEVVDALTFIAHREKAYTMARAIVDKLAEVIPRQLFEVPIQAAIGGKIIARATVKALRKDVLA
KCYGGDVTRKKKLLEKQKEGKKRLKAIGKVEVPQEAFLAVLSAGRDEPKG

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=609, Percent_Identity=51.559934318555, Blast_Score=641, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=43.8848920863309, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI18390331, Length=144, Percent_Identity=38.1944444444444, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=39.3103448275862, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI25306283, Length=137, Percent_Identity=43.0656934306569, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI25306287, Length=137, Percent_Identity=43.0656934306569, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI19923640, Length=137, Percent_Identity=43.0656934306569, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=43.6363636363636, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=43.6363636363636, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=43.6363636363636, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI217272892, Length=143, Percent_Identity=37.0629370629371, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI217272894, Length=143, Percent_Identity=37.0629370629371, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI34147630, Length=255, Percent_Identity=28.2352941176471, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI53729339, Length=261, Percent_Identity=27.5862068965517, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI53729337, Length=261, Percent_Identity=27.5862068965517, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788922, Length=602, Percent_Identity=57.8073089700997, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=518, Percent_Identity=30.8880308880309, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1789738, Length=157, Percent_Identity=35.6687898089172, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1790835, Length=164, Percent_Identity=31.0975609756098, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1789559, Length=221, Percent_Identity=27.6018099547511, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=613, Percent_Identity=40.4567699836868, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17556745, Length=464, Percent_Identity=24.1379310344828, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71988811, Length=132, Percent_Identity=39.3939393939394, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71988819, Length=132, Percent_Identity=39.3939393939394, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=148, Percent_Identity=37.1621621621622, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=178, Percent_Identity=34.8314606741573, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=139, Percent_Identity=34.5323741007194, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=254, Percent_Identity=29.5275590551181, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=603, Percent_Identity=45.1077943615257, Blast_Score=526, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6323098, Length=161, Percent_Identity=37.888198757764, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324707, Length=148, Percent_Identity=41.8918918918919, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=148, Percent_Identity=41.8918918918919, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=140, Percent_Identity=38.5714285714286, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6324166, Length=148, Percent_Identity=37.1621621621622, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=286, Percent_Identity=27.6223776223776, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI78706572, Length=606, Percent_Identity=44.2244224422442, Blast_Score=527, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24582462, Length=149, Percent_Identity=39.5973154362416, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585711, Length=169, Percent_Identity=37.2781065088757, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24585713, Length=169, Percent_Identity=37.2781065088757, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24585709, Length=169, Percent_Identity=37.2781065088757, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=35.5555555555556, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI221458488, Length=140, Percent_Identity=41.4285714285714, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI28574573, Length=137, Percent_Identity=39.4160583941606, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281363316, Length=284, Percent_Identity=26.7605633802817, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17864358, Length=284, Percent_Identity=26.7605633802817, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67601; Mature: 67601

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRMDLSRIRNFSIIAHVDHGKSTLADRILELTHAVSDREMREQFLDSLELERERGITIK
CCCCCHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEE
ASAVRVTYRAKDGEEYVFHLIDTPGHVDFTYEVSRALAAVEGVLLVVDASQGVEAETLAK
EEEEEEEEECCCCCEEEEEEECCCCCEEEHHHHHHHHHHHCCEEEEEECCCCCCHHHHHH
FYMALEHGHVIIPVINKIDLPNARPLEVALEVEEVLGLPADEAIFASGKTGEGVEEILEA
HHHHHHCCCEEEEEECCCCCCCCCCCEEEEEHHHHHCCCCCCCEEECCCCCCCHHHHHHH
IVQRIPPPKGDPEAPLKALIFDSVYDAYQGVIPYLRLFEGRVRPGDRIRIYSTGKEFTVD
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEC
KVGVFTPQGLVATEALEAGEVGWLVAAIRDIHDVQVGDTITLADRPTPSPYPGFRPAKPV
CCEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCE
VFAGLYPVDSGDYGKLRDALEKLKLNDAALTFEPESSTALGFGFRCGFLGLLHAEIVQER
EEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCEECCHHHHHHHHHHHHHHHHHH
LEREFGLSLIATAPSVVYKVRLKSGEEVEVHNPADLPDPTRIEEILEPYVKLTIFTPEEY
HHHHHCCEEEECCCCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHCCHHEEEEECCHHH
VGSLMQLLQEKRGRLVNMNYLPGAQKRVELVYEAPFAEILYDFHDRLKSVSRGYASMDYE
HHHHHHHHHHHCCCEEEEECCCCCHHHHHEEECCCHHHHHHHHHHHHHHHHCCHHCCCHH
QAGYRPGDLVKVNVLVHGEVVDALTFIAHREKAYTMARAIVDKLAEVIPRQLFEVPIQAA
HCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IGGKIIARATVKALRKDVLAKCYGGDVTRKKKLLEKQKEGKKRLKAIGKVEVPQEAFLAV
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LSAGRDEPKG
HHCCCCCCCC
>Mature Secondary Structure
MVRMDLSRIRNFSIIAHVDHGKSTLADRILELTHAVSDREMREQFLDSLELERERGITIK
CCCCCHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEE
ASAVRVTYRAKDGEEYVFHLIDTPGHVDFTYEVSRALAAVEGVLLVVDASQGVEAETLAK
EEEEEEEEECCCCCEEEEEEECCCCCEEEHHHHHHHHHHHCCEEEEEECCCCCCHHHHHH
FYMALEHGHVIIPVINKIDLPNARPLEVALEVEEVLGLPADEAIFASGKTGEGVEEILEA
HHHHHHCCCEEEEEECCCCCCCCCCCEEEEEHHHHHCCCCCCCEEECCCCCCCHHHHHHH
IVQRIPPPKGDPEAPLKALIFDSVYDAYQGVIPYLRLFEGRVRPGDRIRIYSTGKEFTVD
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEC
KVGVFTPQGLVATEALEAGEVGWLVAAIRDIHDVQVGDTITLADRPTPSPYPGFRPAKPV
CCEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCE
VFAGLYPVDSGDYGKLRDALEKLKLNDAALTFEPESSTALGFGFRCGFLGLLHAEIVQER
EEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCEECCHHHHHHHHHHHHHHHHHH
LEREFGLSLIATAPSVVYKVRLKSGEEVEVHNPADLPDPTRIEEILEPYVKLTIFTPEEY
HHHHHCCEEEECCCCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHCCHHEEEEECCHHH
VGSLMQLLQEKRGRLVNMNYLPGAQKRVELVYEAPFAEILYDFHDRLKSVSRGYASMDYE
HHHHHHHHHHHCCCEEEEECCCCCHHHHHEEECCCHHHHHHHHHHHHHHHHCCHHCCCHH
QAGYRPGDLVKVNVLVHGEVVDALTFIAHREKAYTMARAIVDKLAEVIPRQLFEVPIQAA
HCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IGGKIIARATVKALRKDVLAKCYGGDVTRKKKLLEKQKEGKKRLKAIGKVEVPQEAFLAV
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LSAGRDEPKG
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA