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The map label for this gene is murD [H]

Identifier: 54295453

GI number: 54295453

Start: 2896678

End: 2898021

Strand: Reverse

Name: murD [H]

Synonym: lpl2539

Alternate gene names: 54295453

Gene position: 2898021-2896678 (Counterclockwise)

Preceding gene: 54295454

Following gene: 54295452

Centisome position: 86.62

GC content: 43.9

Gene sequence:

>1344_bases
ATGAATCATTCCTTATATTTGGTAGCAGGGCTGGGTAAGACGGGACTCTCTATAGCAAGATACCTGAAGAGAAATAATAA
ATCGTTTGTTGTTTTTGATACACGTAAAGAGGCTCCTGGATTAGCTGAATTCCAAAATGAATTTCCCGATGTGCCAATTT
ACCTGCAACAGACCCCTGATGAAGTAATCAGCCAGGTGACGGATGTTATCACTAGCCCTGGTTTGGCACTGGATACGCCT
GTTTTAGAGAGCGCGCGTCAAGCGGGCGCATTAATTTATGGAGACATAGAATGCCTGGCCAGAGAGATTAGCGCCCCTGT
CATTGCTATAACGGGAACTAATGGTAAATCCACTGTAACGACTTTGGTGGGAGAAATGGCTAAGGCGGCCGGGTTTCGTG
TAGCTGTAGCCGGAAATATCGGCACTCCTGTCTTAGATATGTTGGATGATGAACATCACTACGATTTGTGGGTATTGGAG
TTATCCAGTTTTCAACTGGATTTAACTTATTCTTTGTCGCCAGTGGTTGCGACGATTCTTAATGTAACGCCTGATCATTT
GGACAGGCATCACACTATGGAAGCCTATACGCAAGCAAAACAAAGGATTTATCGTGGTGCCAAGGCTGTTTTATTTAATC
GTGAAGACGTCTACACTGTTCCTCACCAATCCTGTCAAGCAGATATAAAATGCATCTCTTTTGGCAAAGACGCGCCTTCA
ATGGGAAATTGGGGGCTGATTGAACAAGAGAATACTATTTATTTGGCGAAAGGAATGGAGCGTCTTTTGCCAGTGGAATC
GATTTTAATAAAAGGGGTGCATAATTGGATGAATGCGCTTGCTGCTTGTGCTTTAGCCGAAGCAGCGGGAATTTCTATGC
AACACATTCTTAATGTTTTAAAAACCTTCCCTGGACTGCCACATCGATGTCAGTGGGTAAGAGAAGTGGATGGAGTAGGT
TGGATTAATGATTCGAAAGGAACAAATATTGGTGCTACAATCTCTGCTATTAATGGAATAGGAGGTTCTATGCAAGGGAA
AATTGTTCTTATTGCGGGTGGACAGGGAAAAGGGGCGGATTTCCAAGAATTAGCCCAGCCAGTTTCAGAATTTGTTCGTT
CCATTGTTTTGATTGGTGAAGATGCTGACAAGATTGAATCGGCTTTAGCCAAAGTAGTTCCTGTCGTGCGTGCTTCTTCT
CTGGAGGGCGCTGTGACTATTGCCAAGACATGTGCGAAACCTGGTGATGTCGTTTTATTATCTCCAGCTTGCGCCAGTTT
GGATATGTTCCGTGATTTTAATCATAGGGGCGATGTGTTTACATCTTCAGTGCGCGGATTGTGA

Upstream 100 bases:

>100_bases
TGGTCAGAGCCCAAAGTGATAGTAAGATTTTGGATTATTACGGTTGTATTTGTGTTATGCGGATTGGCTACTTTAAAACT
TCGATAGACAAGGTGCATTC

Downstream 100 bases:

>100_bases
ACACGATGCGACCGAGACATTTAAATCAAAGAGGCAAACCCGTTAGCAGACCGATATCACTTTATGATAAATGGCTGATA
GGGGCAGTTTTTGGTTTGCT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MNHSLYLVAGLGKTGLSIARYLKRNNKSFVVFDTRKEAPGLAEFQNEFPDVPIYLQQTPDEVISQVTDVITSPGLALDTP
VLESARQAGALIYGDIECLAREISAPVIAITGTNGKSTVTTLVGEMAKAAGFRVAVAGNIGTPVLDMLDDEHHYDLWVLE
LSSFQLDLTYSLSPVVATILNVTPDHLDRHHTMEAYTQAKQRIYRGAKAVLFNREDVYTVPHQSCQADIKCISFGKDAPS
MGNWGLIEQENTIYLAKGMERLLPVESILIKGVHNWMNALAACALAEAAGISMQHILNVLKTFPGLPHRCQWVREVDGVG
WINDSKGTNIGATISAINGIGGSMQGKIVLIAGGQGKGADFQELAQPVSEFVRSIVLIGEDADKIESALAKVVPVVRASS
LEGAVTIAKTCAKPGDVVLLSPACASLDMFRDFNHRGDVFTSSVRGL

Sequences:

>Translated_447_residues
MNHSLYLVAGLGKTGLSIARYLKRNNKSFVVFDTRKEAPGLAEFQNEFPDVPIYLQQTPDEVISQVTDVITSPGLALDTP
VLESARQAGALIYGDIECLAREISAPVIAITGTNGKSTVTTLVGEMAKAAGFRVAVAGNIGTPVLDMLDDEHHYDLWVLE
LSSFQLDLTYSLSPVVATILNVTPDHLDRHHTMEAYTQAKQRIYRGAKAVLFNREDVYTVPHQSCQADIKCISFGKDAPS
MGNWGLIEQENTIYLAKGMERLLPVESILIKGVHNWMNALAACALAEAAGISMQHILNVLKTFPGLPHRCQWVREVDGVG
WINDSKGTNIGATISAINGIGGSMQGKIVLIAGGQGKGADFQELAQPVSEFVRSIVLIGEDADKIESALAKVVPVVRASS
LEGAVTIAKTCAKPGDVVLLSPACASLDMFRDFNHRGDVFTSSVRGL
>Mature_447_residues
MNHSLYLVAGLGKTGLSIARYLKRNNKSFVVFDTRKEAPGLAEFQNEFPDVPIYLQQTPDEVISQVTDVITSPGLALDTP
VLESARQAGALIYGDIECLAREISAPVIAITGTNGKSTVTTLVGEMAKAAGFRVAVAGNIGTPVLDMLDDEHHYDLWVLE
LSSFQLDLTYSLSPVVATILNVTPDHLDRHHTMEAYTQAKQRIYRGAKAVLFNREDVYTVPHQSCQADIKCISFGKDAPS
MGNWGLIEQENTIYLAKGMERLLPVESILIKGVHNWMNALAACALAEAAGISMQHILNVLKTFPGLPHRCQWVREVDGVG
WINDSKGTNIGATISAINGIGGSMQGKIVLIAGGQGKGADFQELAQPVSEFVRSIVLIGEDADKIESALAKVVPVVRASS
LEGAVTIAKTCAKPGDVVLLSPACASLDMFRDFNHRGDVFTSSVRGL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=445, Percent_Identity=45.1685393258427, Blast_Score=313, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48128; Mature: 48128

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSLYLVAGLGKTGLSIARYLKRNNKSFVVFDTRKEAPGLAEFQNEFPDVPIYLQQTPD
CCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCCCEEEECCHH
EVISQVTDVITSPGLALDTPVLESARQAGALIYGDIECLAREISAPVIAITGTNGKSTVT
HHHHHHHHHHCCCCCEECCHHHHHHHHCCEEEEECHHHHHHHHCCCEEEEECCCCCHHHH
TLVGEMAKAAGFRVAVAGNIGTPVLDMLDDEHHYDLWVLELSSFQLDLTYSLSPVVATIL
HHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCEEEEEEECCEEEEEEECHHHHHHHHH
NVTPDHLDRHHTMEAYTQAKQRIYRGAKAVLFNREDVYTVPHQSCQADIKCISFGKDAPS
HCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCHHHHCCCEEEEECCCCCC
MGNWGLIEQENTIYLAKGMERLLPVESILIKGVHNWMNALAACALAEAAGISMQHILNVL
CCCCCEEECCCEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KTFPGLPHRCQWVREVDGVGWINDSKGTNIGATISAINGIGGSMQGKIVLIAGGQGKGAD
HHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCC
FQELAQPVSEFVRSIVLIGEDADKIESALAKVVPVVRASSLEGAVTIAKTCAKPGDVVLL
HHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEE
SPACASLDMFRDFNHRGDVFTSSVRGL
CCCHHHHHHHHCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MNHSLYLVAGLGKTGLSIARYLKRNNKSFVVFDTRKEAPGLAEFQNEFPDVPIYLQQTPD
CCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCCCEEEECCHH
EVISQVTDVITSPGLALDTPVLESARQAGALIYGDIECLAREISAPVIAITGTNGKSTVT
HHHHHHHHHHCCCCCEECCHHHHHHHHCCEEEEECHHHHHHHHCCCEEEEECCCCCHHHH
TLVGEMAKAAGFRVAVAGNIGTPVLDMLDDEHHYDLWVLELSSFQLDLTYSLSPVVATIL
HHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCEEEEEEECCEEEEEEECHHHHHHHHH
NVTPDHLDRHHTMEAYTQAKQRIYRGAKAVLFNREDVYTVPHQSCQADIKCISFGKDAPS
HCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCHHHHCCCEEEEECCCCCC
MGNWGLIEQENTIYLAKGMERLLPVESILIKGVHNWMNALAACALAEAAGISMQHILNVL
CCCCCEEECCCEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KTFPGLPHRCQWVREVDGVGWINDSKGTNIGATISAINGIGGSMQGKIVLIAGGQGKGAD
HHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCC
FQELAQPVSEFVRSIVLIGEDADKIESALAKVVPVVRASSLEGAVTIAKTCAKPGDVVLL
HHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEE
SPACASLDMFRDFNHRGDVFTSSVRGL
CCCHHHHHHHHCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA