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The map label for this gene is nudE [H]

Identifier: 54295195

GI number: 54295195

Start: 2592933

End: 2593493

Strand: Reverse

Name: nudE [H]

Synonym: lpl2275

Alternate gene names: 54295195

Gene position: 2593493-2592933 (Counterclockwise)

Preceding gene: 54295197

Following gene: 54295194

Centisome position: 77.52

GC content: 43.49

Gene sequence:

>561_bases
ATGCGAAATAAACCAAAATGTAAGCAAAAGACAGTGATAGCCAAATCCCGGTTGTTTACAGTTGAAGAAATGTATCTGGA
ATTTTCCAATGGGGCTCAGCGAGTCTATGAGCGAATTAAAAGTCATGGTCATGGTGCGGTGTTAATAGTTGCATTGACTC
CCGGAGAATCATTATTATTAGTTCGGGAATACGCCGCAGGTACTGATTCTTATGAATTATCATTTCCCAAGGGAGTGATA
GACAGAAATGAAACCCCTATAGAAGCAGCTAACAGAGAGTTACGTGAAGAGACTGGGTTTGCGGCAAGGGAGCTGCACTG
GATTCGTTCCATGTCATTGGCTCCTGGTTATTTTGGAGCTCGCATTGAGCTTATTGTTGCAGAGGGTTTGTACCATTCGC
CGTTGCCAGGCGATGAGCCTGAGCCTCCTGAAACAGTAGAGTGGCCTTTACACGCTGCTTATGAGTTATTGGAAAAACCG
GATTTTACCGAGGCGCGCAGTATTGCTGCGCTATTTTTAGTTAAAGAGTGGTTGAATAAGAGGAAATCATATGACAAATA
A

Upstream 100 bases:

>100_bases
ACAAGGATAACGGAAGCATTACAATTTTTAAACTGGCTTGTTAGGGAATGCAATAGTATCATGGTAGCAATTTTGCTACA
AAATTAAGTGATGACCATTG

Downstream 100 bases:

>100_bases
TCGAGTAAGAGTTGCTGTCACAGGTGCAGCAGGGCAAATAGGTTATGCCCTGGTGTTTCGTATTGCTTCCGGTCAAATGT
TTGGGCCAAATACTGAAGTG

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MRNKPKCKQKTVIAKSRLFTVEEMYLEFSNGAQRVYERIKSHGHGAVLIVALTPGESLLLVREYAAGTDSYELSFPKGVI
DRNETPIEAANRELREETGFAARELHWIRSMSLAPGYFGARIELIVAEGLYHSPLPGDEPEPPETVEWPLHAAYELLEKP
DFTEARSIAALFLVKEWLNKRKSYDK

Sequences:

>Translated_186_residues
MRNKPKCKQKTVIAKSRLFTVEEMYLEFSNGAQRVYERIKSHGHGAVLIVALTPGESLLLVREYAAGTDSYELSFPKGVI
DRNETPIEAANRELREETGFAARELHWIRSMSLAPGYFGARIELIVAEGLYHSPLPGDEPEPPETVEWPLHAAYELLEKP
DFTEARSIAALFLVKEWLNKRKSYDK
>Mature_186_residues
MRNKPKCKQKTVIAKSRLFTVEEMYLEFSNGAQRVYERIKSHGHGAVLIVALTPGESLLLVREYAAGTDSYELSFPKGVI
DRNETPIEAANRELREETGFAARELHWIRSMSLAPGYFGARIELIVAEGLYHSPLPGDEPEPPETVEWPLHAAYELLEKP
DFTEARSIAALFLVKEWLNKRKSYDK

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=176, Percent_Identity=52.8409090909091, Blast_Score=192, Evalue=8e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 21147; Mature: 21147

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKPKCKQKTVIAKSRLFTVEEMYLEFSNGAQRVYERIKSHGHGAVLIVALTPGESLLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEE
VREYAAGTDSYELSFPKGVIDRNETPIEAANRELREETGFAARELHWIRSMSLAPGYFGA
EEEHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCC
RIELIVAEGLYHSPLPGDEPEPPETVEWPLHAAYELLEKPDFTEARSIAALFLVKEWLNK
EEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RKSYDK
HCCCCC
>Mature Secondary Structure
MRNKPKCKQKTVIAKSRLFTVEEMYLEFSNGAQRVYERIKSHGHGAVLIVALTPGESLLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEE
VREYAAGTDSYELSFPKGVIDRNETPIEAANRELREETGFAARELHWIRSMSLAPGYFGA
EEEHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCC
RIELIVAEGLYHSPLPGDEPEPPETVEWPLHAAYELLEKPDFTEARSIAALFLVKEWLNK
EEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RKSYDK
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]