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The map label for this gene is mdh [H]

Identifier: 54295194

GI number: 54295194

Start: 2591951

End: 2592943

Strand: Reverse

Name: mdh [H]

Synonym: lpl2274

Alternate gene names: 54295194

Gene position: 2592943-2591951 (Counterclockwise)

Preceding gene: 54295195

Following gene: 54295192

Centisome position: 77.5

GC content: 43.3

Gene sequence:

>993_bases
ATGACAAATAATCGAGTAAGAGTTGCTGTCACAGGTGCAGCAGGGCAAATAGGTTATGCCCTGGTGTTTCGTATTGCTTC
CGGTCAAATGTTTGGGCCAAATACTGAAGTGGAATTGAATTTACTTGAACTTGAGCCTGCACTTCCCTCACTGGAAGGAG
TCGCTATGGAGCTTGATGATTGTGCATTTCCCTTACTAAAACGCATTGTTTGTACTGCAGATTTGAATAAGGCAATGGAT
GGTGTTAACTGGGCGTTATTAGTCGGTTCAGTTCCGCGAAAGCAAGGCATGGAACGTTCTGATTTATTACAAATCAATGG
TGGTATATTCACCAAACAAGGTCAGGCGATTAATGATTATGCTAGCGATGATGTACGGGTATTTGTTGTGGGAAACCCTT
GCAATACCAATTGCCTGATTGCTATGAATCACGCAAAAGACGTGCCTTCTGATCGTTTTTATGCAATGACCACATTGGAT
GAGTTAAGAGCTCGTACTCAATTGGCTAAAAAAGCAGGCGTCGACATTACTGCAGTGACTCAAATGACGATTTGGGGTAA
TCATTCCGCCACCCAATATCCCGATTTTTATAACGCAAAAATCAATGGAACTTCTGCTGCGCGAGTCATAAATGATCAAA
CCTGGTTAAAAGAGACTTTTGTATCAACCGTCCAGCAGCGAGGAGCCGCAGTGATCAAGGCACGAGGTTCGTCTTCTGCC
GCTTCTGCTGCCAATGCGATAATTACAGGAGTTAATCATTTGGTTACCGATACTCCTGCTGGCGAATCGTTTTCAATGTG
CCGTCGCTCAAAGGGAGAGTATGGAGTCGATGAAGGTTTGATTTTCTCTTTCCCATGCCGAAGAGAACATGGCGAATTAA
AAGTGGTTGAGAACATGGAGTTCAACGATTTTGGACGCGAGAGATTCAATATCACTTTAAACGAATTACGAAGTGAGCGT
GACACGGTCAAGTCTTTAGGTTTATTGGATTAA

Upstream 100 bases:

>100_bases
CACGCTGCTTATGAGTTATTGGAAAAACCGGATTTTACCGAGGCGCGCAGTATTGCTGCGCTATTTTTAGTTAAAGAGTG
GTTGAATAAGAGGAAATCAT

Downstream 100 bases:

>100_bases
CCAGACCGGGTCAATTCTTGATAGATTTGATCCTGTTGTTTTTGCTGATTACCCTGCTTTGTGAATTTTTCAGGGCAGGG
TGCTGTCGCACCTTGGTTTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTNNRVRVAVTGAAGQIGYALVFRIASGQMFGPNTEVELNLLELEPALPSLEGVAMELDDCAFPLLKRIVCTADLNKAMD
GVNWALLVGSVPRKQGMERSDLLQINGGIFTKQGQAINDYASDDVRVFVVGNPCNTNCLIAMNHAKDVPSDRFYAMTTLD
ELRARTQLAKKAGVDITAVTQMTIWGNHSATQYPDFYNAKINGTSAARVINDQTWLKETFVSTVQQRGAAVIKARGSSSA
ASAANAIITGVNHLVTDTPAGESFSMCRRSKGEYGVDEGLIFSFPCRREHGELKVVENMEFNDFGRERFNITLNELRSER
DTVKSLGLLD

Sequences:

>Translated_330_residues
MTNNRVRVAVTGAAGQIGYALVFRIASGQMFGPNTEVELNLLELEPALPSLEGVAMELDDCAFPLLKRIVCTADLNKAMD
GVNWALLVGSVPRKQGMERSDLLQINGGIFTKQGQAINDYASDDVRVFVVGNPCNTNCLIAMNHAKDVPSDRFYAMTTLD
ELRARTQLAKKAGVDITAVTQMTIWGNHSATQYPDFYNAKINGTSAARVINDQTWLKETFVSTVQQRGAAVIKARGSSSA
ASAANAIITGVNHLVTDTPAGESFSMCRRSKGEYGVDEGLIFSFPCRREHGELKVVENMEFNDFGRERFNITLNELRSER
DTVKSLGLLD
>Mature_329_residues
TNNRVRVAVTGAAGQIGYALVFRIASGQMFGPNTEVELNLLELEPALPSLEGVAMELDDCAFPLLKRIVCTADLNKAMDG
VNWALLVGSVPRKQGMERSDLLQINGGIFTKQGQAINDYASDDVRVFVVGNPCNTNCLIAMNHAKDVPSDRFYAMTTLDE
LRARTQLAKKAGVDITAVTQMTIWGNHSATQYPDFYNAKINGTSAARVINDQTWLKETFVSTVQQRGAAVIKARGSSSAA
SAANAIITGVNHLVTDTPAGESFSMCRRSKGEYGVDEGLIFSFPCRREHGELKVVENMEFNDFGRERFNITLNELRSERD
TVKSLGLLD

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family [H]

Homologues:

Organism=Homo sapiens, GI5174539, Length=322, Percent_Identity=47.2049689440994, Blast_Score=292, Evalue=3e-79,
Organism=Homo sapiens, GI89886456, Length=310, Percent_Identity=23.8709677419355, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1789632, Length=277, Percent_Identity=27.0758122743682, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17561064, Length=321, Percent_Identity=46.417445482866, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI32566798, Length=235, Percent_Identity=47.6595744680851, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=43.5294117647059, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24583394, Length=329, Percent_Identity=47.112462006079, Blast_Score=281, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24647881, Length=331, Percent_Identity=26.2839879154079, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010945
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 36064; Mature: 35933

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNRVRVAVTGAAGQIGYALVFRIASGQMFGPNTEVELNLLELEPALPSLEGVAMELDD
CCCCEEEEEEECCCCCCCEEEEEEEECCCEECCCCCEEEEEEEECCCCCCCCCEEEEHHH
CAFPLLKRIVCTADLNKAMDGVNWALLVGSVPRKQGMERSDLLQINGGIFTKQGQAINDY
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHEEEECCCEEECCCCCCCCC
ASDDVRVFVVGNPCNTNCLIAMNHAKDVPSDRFYAMTTLDELRARTQLAKKAGVDITAVT
CCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEE
QMTIWGNHSATQYPDFYNAKINGTSAARVINDQTWLKETFVSTVQQRGAAVIKARGSSSA
EEEEECCCCCCCCCCCEEEEECCCHHEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCH
ASAANAIITGVNHLVTDTPAGESFSMCRRSKGEYGVDEGLIFSFPCRREHGELKVVENME
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCC
FNDFGRERFNITLNELRSERDTVKSLGLLD
CCCCCCEEEEEEHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TNNRVRVAVTGAAGQIGYALVFRIASGQMFGPNTEVELNLLELEPALPSLEGVAMELDD
CCCEEEEEEECCCCCCCEEEEEEEECCCEECCCCCEEEEEEEECCCCCCCCCEEEEHHH
CAFPLLKRIVCTADLNKAMDGVNWALLVGSVPRKQGMERSDLLQINGGIFTKQGQAINDY
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHEEEECCCEEECCCCCCCCC
ASDDVRVFVVGNPCNTNCLIAMNHAKDVPSDRFYAMTTLDELRARTQLAKKAGVDITAVT
CCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEE
QMTIWGNHSATQYPDFYNAKINGTSAARVINDQTWLKETFVSTVQQRGAAVIKARGSSSA
EEEEECCCCCCCCCCCEEEEECCCHHEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCH
ASAANAIITGVNHLVTDTPAGESFSMCRRSKGEYGVDEGLIFSFPCRREHGELKVVENME
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCC
FNDFGRERFNITLNELRSERDTVKSLGLLD
CCCCCCEEEEEEHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA