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The map label for this gene is exoA [H]

Identifier: 54293631

GI number: 54293631

Start: 764146

End: 764928

Strand: Direct

Name: exoA [H]

Synonym: lpl0684

Alternate gene names: 54293631

Gene position: 764146-764928 (Clockwise)

Preceding gene: 54293630

Following gene: 54293632

Centisome position: 22.84

GC content: 39.72

Gene sequence:

>783_bases
ATGAAAGTAATTAGTTTTAACGCCAATGGCATACGCTCTTCTGCCCGTAATGGATTTTATGATTGGCTTGTAGGGCAGGA
TGTTGATTTTGTATGTATTCAGGAAACGAAATCGCAAGTAGAGCAATTAATCCCGGAAGAACTTTATTTTCCAAGGGATT
ATTTTTGTGATTATTTTGATGCTGAAAAGAAGGGCTACAGTGGGGTTGCCATTTATTCAAAATATAAACCCGTGCGTATT
GTGAAGGGGTTGGGGTTTGATTATTGTGATAAAGAAGGGCGTTATATCCAATTTGATTACCCTAAATTAAGTGTGATTTC
TCTTTATTTGCCTTCTGGAACAAGTGGTGAGGATCGGCAAGAAGTGAAATATGCTTTTCTGGAGCAATTTGCCAGACACT
TGATGAATTTAAAAAATGAGGGAAGAGAGTTAATTATTTGTGGTGATTACAACATCGCGCATAAAAAGATAGATTTAAAA
AATTGGCGAGGGAACCAGAAAAACTCAGGATTTTTACCCGAAGAACGTGCCTGGATGGATGAGCTATTTGGTTCTATGGG
GTTTGTAGACGCATTTAGGGTACATAATCAGGAAGAAGAGCAATATACCTGGTGGTCTTATCGAGCTAGAGCCTGGGAAA
AGAATGTCGGGTGGCGGATTGATTATCAGGTGATTACGCCTGGTCTGACTGAATATGTCGCGGACAGCCGAATTTTTCGT
GAAGCACGTTTTTCGGATCATGCCCCTTTAATGATTGAATACAAGGGAGACTGGTGTGTTTAA

Upstream 100 bases:

>100_bases
GGAGACGTACTTGAGAGTATTGTCAGATGCCTGGAAGAATCATAAGGTTGATAAGATTAACATTTAACTGTTCAGTAATG
ATAGCGGAGTAGCCTAAGTA

Downstream 100 bases:

>100_bases
ACTCGCTAGCTGGAATGTTAATTCATTAAAAGTCCGTCTGGAACAGGTTTTGCAATGGCTTGAGTCTTCACAAGTTGATG
TGCTTGCCATGCAGGAGACT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKVISFNANGIRSSARNGFYDWLVGQDVDFVCIQETKSQVEQLIPEELYFPRDYFCDYFDAEKKGYSGVAIYSKYKPVRI
VKGLGFDYCDKEGRYIQFDYPKLSVISLYLPSGTSGEDRQEVKYAFLEQFARHLMNLKNEGRELIICGDYNIAHKKIDLK
NWRGNQKNSGFLPEERAWMDELFGSMGFVDAFRVHNQEEEQYTWWSYRARAWEKNVGWRIDYQVITPGLTEYVADSRIFR
EARFSDHAPLMIEYKGDWCV

Sequences:

>Translated_260_residues
MKVISFNANGIRSSARNGFYDWLVGQDVDFVCIQETKSQVEQLIPEELYFPRDYFCDYFDAEKKGYSGVAIYSKYKPVRI
VKGLGFDYCDKEGRYIQFDYPKLSVISLYLPSGTSGEDRQEVKYAFLEQFARHLMNLKNEGRELIICGDYNIAHKKIDLK
NWRGNQKNSGFLPEERAWMDELFGSMGFVDAFRVHNQEEEQYTWWSYRARAWEKNVGWRIDYQVITPGLTEYVADSRIFR
EARFSDHAPLMIEYKGDWCV
>Mature_260_residues
MKVISFNANGIRSSARNGFYDWLVGQDVDFVCIQETKSQVEQLIPEELYFPRDYFCDYFDAEKKGYSGVAIYSKYKPVRI
VKGLGFDYCDKEGRYIQFDYPKLSVISLYLPSGTSGEDRQEVKYAFLEQFARHLMNLKNEGRELIICGDYNIAHKKIDLK
NWRGNQKNSGFLPEERAWMDELFGSMGFVDAFRVHNQEEEQYTWWSYRARAWEKNVGWRIDYQVITPGLTEYVADSRIFR
EARFSDHAPLMIEYKGDWCV

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=255, Percent_Identity=35.2941176470588, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI18375503, Length=255, Percent_Identity=35.2941176470588, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI18375501, Length=255, Percent_Identity=35.2941176470588, Blast_Score=168, Evalue=4e-42,
Organism=Escherichia coli, GI1788046, Length=276, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71989536, Length=255, Percent_Identity=32.156862745098, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI221330655, Length=253, Percent_Identity=35.5731225296443, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI17136678, Length=253, Percent_Identity=35.5731225296443, Blast_Score=144, Evalue=5e-35,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30677; Mature: 30677

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVISFNANGIRSSARNGFYDWLVGQDVDFVCIQETKSQVEQLIPEELYFPRDYFCDYFD
CEEEEECCCCCHHHHCCCCEEEECCCCCCEEEHHHHHHHHHHHCHHHHCCCHHHHCCCCC
AEKKGYSGVAIYSKYKPVRIVKGLGFDYCDKEGRYIQFDYPKLSVISLYLPSGTSGEDRQ
CCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCHHH
EVKYAFLEQFARHLMNLKNEGRELIICGDYNIAHKKIDLKNWRGNQKNSGFLPEERAWMD
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHH
ELFGSMGFVDAFRVHNQEEEQYTWWSYRARAWEKNVGWRIDYQVITPGLTEYVADSRIFR
HHHHHCCCHHHHHHCCCCCCCEEHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHH
EARFSDHAPLMIEYKGDWCV
HHCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MKVISFNANGIRSSARNGFYDWLVGQDVDFVCIQETKSQVEQLIPEELYFPRDYFCDYFD
CEEEEECCCCCHHHHCCCCEEEECCCCCCEEEHHHHHHHHHHHCHHHHCCCHHHHCCCCC
AEKKGYSGVAIYSKYKPVRIVKGLGFDYCDKEGRYIQFDYPKLSVISLYLPSGTSGEDRQ
CCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCHHH
EVKYAFLEQFARHLMNLKNEGRELIICGDYNIAHKKIDLKNWRGNQKNSGFLPEERAWMD
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHH
ELFGSMGFVDAFRVHNQEEEQYTWWSYRARAWEKNVGWRIDYQVITPGLTEYVADSRIFR
HHHHHCCCHHHHHHCCCCCCCEEHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHH
EARFSDHAPLMIEYKGDWCV
HHCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]