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The map label for this gene is xthA [H]

Identifier: 54293632

GI number: 54293632

Start: 764921

End: 765691

Strand: Direct

Name: xthA [H]

Synonym: lpl0685

Alternate gene names: 54293632

Gene position: 764921-765691 (Clockwise)

Preceding gene: 54293631

Following gene: 54293633

Centisome position: 22.86

GC content: 40.86

Gene sequence:

>771_bases
GTGTTTAAACTCGCTAGCTGGAATGTTAATTCATTAAAAGTCCGTCTGGAACAGGTTTTGCAATGGCTTGAGTCTTCACA
AGTTGATGTGCTTGCCATGCAGGAGACTAAGCTGACTGATGAGAACTTTCCTGTTGTTTCATTTACAGACAAAGGGTACC
ACGTCATTTTTTCAGGTCAGAAAACTTATAACGGGGTTGCCATTATTAGTCGATATCCTATGACAGATATTCAAACCGAT
ATTCCAGAATTGGAAGATCCCCAGCGGCGTATCCTGGCTGCTACAGTAAAAGGAATCAGATTGATTAATCTTTATGTGCC
TAATGGTGGAGAACTGACTTCAGACAAGTACCAATATAAACTGGATTGGTTAAATAAAGTGACTCGTTTCATTCAACATG
AGATAAGCATTTATTCTAAAGTAGCTGTTGTGGGTGATTTTAATATTGCGCCCGAAGATCGGGATGTCCATGATCCAGTT
GAGTGGGAGGGTTGTGTTTTGGTCAGCCCTCCTGAAAGGCAAGCATTTATGAAGATACTTGAATTGGGTTTACATGACAG
TTTCCGTAATTTTGCTCAGGAAGAAAAAGCGTTTAGCTGGTGGGATTATAGAGCCGCAGCATTCCGACGTAATCGTGGTT
TGCGCATTGATCATGTGTTATTGAGCCATGAATTAAATGCTTTATGTCGACAATCCAAAATCGATAAAGAACCAAGAAAA
GCAGAGAGGCCTTCCGATCATGCGCCGGTTTGGGTTGAGTTAGAATTATAA

Upstream 100 bases:

>100_bases
ACGCCTGGTCTGACTGAATATGTCGCGGACAGCCGAATTTTTCGTGAAGCACGTTTTTCGGATCATGCCCCTTTAATGAT
TGAATACAAGGGAGACTGGT

Downstream 100 bases:

>100_bases
ATGTCTGAGGTGTTTGAGAAAAAATTGCTTTATGAGATCATTTCTGCTATTGTTTTGACTCATTTGTGTAGAGTATGGTG
CTTGGCAATAGCGTCCAGGT

Product: hypothetical protein

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MFKLASWNVNSLKVRLEQVLQWLESSQVDVLAMQETKLTDENFPVVSFTDKGYHVIFSGQKTYNGVAIISRYPMTDIQTD
IPELEDPQRRILAATVKGIRLINLYVPNGGELTSDKYQYKLDWLNKVTRFIQHEISIYSKVAVVGDFNIAPEDRDVHDPV
EWEGCVLVSPPERQAFMKILELGLHDSFRNFAQEEKAFSWWDYRAAAFRRNRGLRIDHVLLSHELNALCRQSKIDKEPRK
AERPSDHAPVWVELEL

Sequences:

>Translated_256_residues
MFKLASWNVNSLKVRLEQVLQWLESSQVDVLAMQETKLTDENFPVVSFTDKGYHVIFSGQKTYNGVAIISRYPMTDIQTD
IPELEDPQRRILAATVKGIRLINLYVPNGGELTSDKYQYKLDWLNKVTRFIQHEISIYSKVAVVGDFNIAPEDRDVHDPV
EWEGCVLVSPPERQAFMKILELGLHDSFRNFAQEEKAFSWWDYRAAAFRRNRGLRIDHVLLSHELNALCRQSKIDKEPRK
AERPSDHAPVWVELEL
>Mature_256_residues
MFKLASWNVNSLKVRLEQVLQWLESSQVDVLAMQETKLTDENFPVVSFTDKGYHVIFSGQKTYNGVAIISRYPMTDIQTD
IPELEDPQRRILAATVKGIRLINLYVPNGGELTSDKYQYKLDWLNKVTRFIQHEISIYSKVAVVGDFNIAPEDRDVHDPV
EWEGCVLVSPPERQAFMKILELGLHDSFRNFAQEEKAFSWWDYRAAAFRRNRGLRIDHVLLSHELNALCRQSKIDKEPRK
AERPSDHAPVWVELEL

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=264, Percent_Identity=29.5454545454545, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI18375503, Length=264, Percent_Identity=29.5454545454545, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI18375501, Length=264, Percent_Identity=29.5454545454545, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1788046, Length=264, Percent_Identity=35.2272727272727, Blast_Score=154, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI71989536, Length=262, Percent_Identity=26.3358778625954, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI221330655, Length=266, Percent_Identity=27.4436090225564, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI17136678, Length=266, Percent_Identity=27.4436090225564, Blast_Score=87, Evalue=8e-18,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29730; Mature: 29730

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKLASWNVNSLKVRLEQVLQWLESSQVDVLAMQETKLTDENFPVVSFTDKGYHVIFSGQ
CCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEECCCEEEEEECC
KTYNGVAIISRYPMTDIQTDIPELEDPQRRILAATVKGIRLINLYVPNGGELTSDKYQYK
CCCCCEEEEEECCCCCHHCCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCEEE
LDWLNKVTRFIQHEISIYSKVAVVGDFNIAPEDRDVHDPVEWEGCVLVSPPERQAFMKIL
HHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHH
ELGLHDSFRNFAQEEKAFSWWDYRAAAFRRNRGLRIDHVLLSHELNALCRQSKIDKEPRK
HHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCHH
AERPSDHAPVWVELEL
HCCCCCCCCEEEEEEC
>Mature Secondary Structure
MFKLASWNVNSLKVRLEQVLQWLESSQVDVLAMQETKLTDENFPVVSFTDKGYHVIFSGQ
CCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEECCCEEEEEECC
KTYNGVAIISRYPMTDIQTDIPELEDPQRRILAATVKGIRLINLYVPNGGELTSDKYQYK
CCCCCEEEEEECCCCCHHCCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCEEE
LDWLNKVTRFIQHEISIYSKVAVVGDFNIAPEDRDVHDPVEWEGCVLVSPPERQAFMKIL
HHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHH
ELGLHDSFRNFAQEEKAFSWWDYRAAAFRRNRGLRIDHVLLSHELNALCRQSKIDKEPRK
HHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCHH
AERPSDHAPVWVELEL
HCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]