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The map label for this gene is nifS

Identifier: 54020394

GI number: 54020394

Start: 245509

End: 246654

Strand: Reverse

Name: nifS

Synonym: mhp217

Alternate gene names: 54020394

Gene position: 246654-245509 (Counterclockwise)

Preceding gene: 54020395

Following gene: 54020393

Centisome position: 27.63

GC content: 26.96

Gene sequence:

>1146_bases
ATGACTAACTTTAAAAATAATTTTCCTTTTCTTAACAAGGTTACTTACCTTGATTCTGCAGCAATGATGCAAAAACCACT
TTCTGTAATTAAAAAAATTAGTGACTTTTACTTAAATTCAGCTATAAATATTCATTCACAGAATTCAAAAATAGGCTATG
AAAATAGTGATAAAATCGGAAAAATTAGGGAAAAAATTGCCAGATTTATCAATGCCGAATCAGAAGAAATTATTTTTAAT
TCTGGAACAACCGAAGCAATTAACTTATTTGCTAATATGATTAAAAAATTCCTAAAAAAGGGTGATCAGATTTTACTTTC
GCCTCTAAATCATTCATCAAATTTAATAATTTGAATTAAAATAGCAGCTAAAATAGGTGCCGAAATTATTTATAGTAAAA
AAATAATTGATAAAATTTCACCAAAAACTAAAATTATTGCTTTTTCACAAACGAATAATTCAATTTTAGTTAATCTGAAT
TTGGTGGAGATTTGGAAAAAGGCTGTAAAATACGATGCTTTTGTATTTAATGATGCTACTCAGGCAATAAATTATCAAAA
AGTTAGTCTGAAATTATGTCATGGTCTTGCTTTCAGTTCAAATAAATTTTATGGGCCGACCGGCCTTGGAGTCTTAGCAA
TAAAAAAAGAACTTCTTAATAAATTAGAACCAGTTAAATTTGGAGGAGGAACAGTTGCAGAAATAAGTTCTAAAAATATA
GTTTATTATAATGGTTATCGAAAATTTGAGGCAGGAACCTTGAATTTAGCAGCAATTTGGGGCCTTGGTGCTGCAGTTGA
TTTTATAAATGCTATCGGAATTAAAAAAATTGCCAAAAAACTACAAGTTTTAAGTATTTATTTATACGAAAAACTTGAAA
AAATTGAAAAAATTGAAATTTTTTCTAAAAAGGGCAATCATATTATAATTTTTAATATTAAAGAATTTAACGCATCGGAT
GTTGTCTCATATCTAGGAAATAATAATATTTATGTTCGCAGTGGGACGTTTTGCGTTCCTTTTTTAGAAAAAATACTAAA
GAAAAGAAGCTTTATTCGTGTTTCACTAGCATTTTACAATGATTTTAATGATATTGATAGACTAATTTCGGTTTTAGAAA
AAAAGGAGTTTTTGAATTTTGTATAA

Upstream 100 bases:

>100_bases
TTTGCAAAACCAAGTTTTGAATTTCTCAATTTTTTTGAATCACCAATTAAAGGACAAAAATCCAAAAATACAGAGTATTT
TTTACATTTTATTAGAAAGA

Downstream 100 bases:

>100_bases
GGATTTTAGCAAAAGGCGTGATATTATTTTAAGTTCAAATAAAAAATTTAATGAAAAAAAACGAATTTGTCTAACAAAAA
ATAGTCAAATAAATGAAAAT

Product: nitrogen fixation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTNFKNNFPFLNKVTYLDSAAMMQKPLSVIKKISDFYLNSAINIHSQNSKIGYENSDKIGKIREKIARFINAESEEIIFN
SGTTEAINLFANMIKKFLKKGDQILLSPLNHSSNLIIWIKIAAKIGAEIIYSKKIIDKISPKTKIIAFSQTNNSILVNLN
LVEIWKKAVKYDAFVFNDATQAINYQKVSLKLCHGLAFSSNKFYGPTGLGVLAIKKELLNKLEPVKFGGGTVAEISSKNI
VYYNGYRKFEAGTLNLAAIWGLGAAVDFINAIGIKKIAKKLQVLSIYLYEKLEKIEKIEIFSKKGNHIIIFNIKEFNASD
VVSYLGNNNIYVRSGTFCVPFLEKILKKRSFIRVSLAFYNDFNDIDRLISVLEKKEFLNFV

Sequences:

>Translated_381_residues
MTNFKNNFPFLNKVTYLDSAAMMQKPLSVIKKISDFYLNSAINIHSQNSKIGYENSDKIGKIREKIARFINAESEEIIFN
SGTTEAINLFANMIKKFLKKGDQILLSPLNHSSNLII*IKIAAKIGAEIIYSKKIIDKISPKTKIIAFSQTNNSILVNLN
LVEIWKKAVKYDAFVFNDATQAINYQKVSLKLCHGLAFSSNKFYGPTGLGVLAIKKELLNKLEPVKFGGGTVAEISSKNI
VYYNGYRKFEAGTLNLAAIWGLGAAVDFINAIGIKKIAKKLQVLSIYLYEKLEKIEKIEIFSKKGNHIIIFNIKEFNASD
VVSYLGNNNIYVRSGTFCVPFLEKILKKRSFIRVSLAFYNDFNDIDRLISVLEKKEFLNFV
>Mature_380_residues
TNFKNNFPFLNKVTYLDSAAMMQKPLSVIKKISDFYLNSAINIHSQNSKIGYENSDKIGKIREKIARFINAESEEIIFNS
GTTEAINLFANMIKKFLKKGDQILLSPLNHSSNLII*IKIAAKIGAEIIYSKKIIDKISPKTKIIAFSQTNNSILVNLNL
VEIWKKAVKYDAFVFNDATQAINYQKVSLKLCHGLAFSSNKFYGPTGLGVLAIKKELLNKLEPVKFGGGTVAEISSKNIV
YYNGYRKFEAGTLNLAAIWGLGAAVDFINAIGIKKIAKKLQVLSIYLYEKLEKIEKIEIFSKKGNHIIIFNIKEFNASDV
VSYLGNNNIYVRSGTFCVPFLEKILKKRSFIRVSLAFYNDFNDIDRLISVLEKKEFLNFV

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine [H]

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=276, Percent_Identity=25, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1787970, Length=392, Percent_Identity=31.8877551020408, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1789175, Length=391, Percent_Identity=29.4117647058824, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI48994898, Length=264, Percent_Identity=26.1363636363636, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=319, Percent_Identity=24.4514106583072, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319831, Length=299, Percent_Identity=27.0903010033445, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI20129463, Length=267, Percent_Identity=26.5917602996255, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42877; Mature: 42746

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNFKNNFPFLNKVTYLDSAAMMQKPLSVIKKISDFYLNSAINIHSQNSKIGYENSDKIG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHH
KIREKIARFINAESEEIIFNSGTTEAINLFANMIKKFLKKGDQILLSPLNHSSNLIIIKI
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEE
AAKIGAEIIYSKKIIDKISPKTKIIAFSQTNNSILVNLNLVEIWKKAVKYDAFVFNDATQ
HHHHCHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEHHHHHHHHHHHHHHEEECCHHH
AINYQKVSLKLCHGLAFSSNKFYGPTGLGVLAIKKELLNKLEPVKFGGGTVAEISSKNIV
HCCHHHHHHHHHHCEEECCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEE
YYNGYRKFEAGTLNLAAIWGLGAAVDFINAIGIKKIAKKLQVLSIYLYEKLEKIEKIEIF
EECCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
SKKGNHIIIFNIKEFNASDVVSYLGNNNIYVRSGTFCVPFLEKILKKRSFIRVSLAFYND
ECCCCEEEEEEEECCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCEEEEEEEECCC
FNDIDRLISVLEKKEFLNFV
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNFKNNFPFLNKVTYLDSAAMMQKPLSVIKKISDFYLNSAINIHSQNSKIGYENSDKIG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHH
KIREKIARFINAESEEIIFNSGTTEAINLFANMIKKFLKKGDQILLSPLNHSSNLIIIKI
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEE
AAKIGAEIIYSKKIIDKISPKTKIIAFSQTNNSILVNLNLVEIWKKAVKYDAFVFNDATQ
HHHHCHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEHHHHHHHHHHHHHHEEECCHHH
AINYQKVSLKLCHGLAFSSNKFYGPTGLGVLAIKKELLNKLEPVKFGGGTVAEISSKNIV
HCCHHHHHHHHHHCEEECCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEE
YYNGYRKFEAGTLNLAAIWGLGAAVDFINAIGIKKIAKKLQVLSIYLYEKLEKIEKIEIF
EECCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
SKKGNHIIIFNIKEFNASDVVSYLGNNNIYVRSGTFCVPFLEKILKKRSFIRVSLAFYND
ECCCCEEEEEEEECCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCEEEEEEEECCC
FNDIDRLISVLEKKEFLNFV
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]