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The map label for this gene is mleA [H]

Identifier: 52787814

GI number: 52787814

Start: 3963426

End: 3964757

Strand: Reverse

Name: mleA [H]

Synonym: BLi04137

Alternate gene names: 52787814

Gene position: 3964757-3963426 (Counterclockwise)

Preceding gene: 52787815

Following gene: 52787811

Centisome position: 93.89

GC content: 52.7

Gene sequence:

>1332_bases
ATGAATGGCAACAATATTATTCGGAATTTGATGCTGGAAATCCCTTCAGCACCCGGAAACCTTGGAAAGGTGACAACGGC
GATCGGCCTTGCCGGCGGTGATATCGGAGAGATTGAAACGATCAAGGTAGGGCCGAATTATACGATGCGGAACATTACGG
TTCAAGTTGAGCATGAAGAACAGCTCACCGAGATTCTTGAAGTGATCCGCAACTTAAAAGAAGGCATCCATCTTCATACC
GTTTCAGATGACGTGCTGGCTGCACATGAAGGCGGCAAAATCCGCATGAAAAGCAAGATGCCGATTCGCTCGCTTGCTGA
TCTGCGTCGGGTGTATACGCCGGGTGTCGCGAACGTGTGCGAGCTCATTAAAGAAGAGCCGCAAAAAGCGAATATTTATA
CCGGAATCGGCAATTCGGTTGCAATTGTAACGGACGGCACGGCCATCCTTGGCTTGGGAGACATAGGGCCAGTTGCTGGA
ATGCCTGTTATGGAAGGGAAAGCCGCTCTTTTCGACCAGCTTGCGGATATCAGCGGGGTTCCGATTCTGCTCGACACGAA
TGATCCTGATGAAATCGTAAGGACGGTTAAAAACATCGCGCCCGGCTTTAGCGGCATTTTGCTTGAAGACATCGGTTCAC
CGCATTGTTTTGAAATTGAAGAGCGCCTGAAAAACGACCTGAATATTCCGGTCATGCATGACGACCAGCACGGTACGGCG
GTTGTGACGCTGGCGGCCGCCATTTCAGCAGCCAAGAGTGCAGGGGTGGATCTGAAGCAGGCCCATGTCGGCCAAATCGG
CCTGGGCGCGGCGGGTGTCGCGATCTGCAGGATGTTTATGGCATATGGAGTAAAACGTGTTGTCGGAACGGATAAATCGC
TTGAGGCGATGGCGCGTTTAGAGAACTATGGCGGCCATGCGGCTGAAAGCATCGAAGAATTGATGGAAAGCTGCGATATC
GTCGTTGCGACAACCGGTGTTCCGGGATTGATCAATAAGAAATGGGTTCGGGAAGGCCAGGTGATTTTGGCTCTGTCCAA
TCCGAAGCCGGAGATTGAGCCTGAGGATGCGCTCGAGGCCGGGGCAGCCTACGCTGCAGACGGACGGTCTGTCAACAATG
TGCTGGGGTTCCCAGGTATTTTCAGGGGCGTGTTAAATGCCGGAGCCGATTCGATCAGCCACCCGATGCTGGTTGCGGCC
GCTGAAGCGATCGCATCCTGCACAAGACAAGGCGATCTTGTGCCTCATCCGCTGAACCCTGAAGTTCACGAGATTGTGGC
CAAAGCAGTCGAAAGGGTCGCACTTTCAGAAATGGGAAAAATCAACGGATAA

Upstream 100 bases:

>100_bases
ATTTTGTACGGATACACAGGTAAATTTATTTGGTACACCGATAAAAAAGACTCGCCGGTTCAGTAGCCGATTTTAAAACA
ATTGATGGAGGTATTCTGTT

Downstream 100 bases:

>100_bases
AGAAAACAGCTGCTGCTTCATCGCAATACGGATAAAGCGGCAGCTGTTTTTTTCGCGAATGTTAACGGCGGTTCTACAGA
TTGAAAATGAATATATAAAA

Product: MleA

Products: NA

Alternate protein names: NAD-ME 1 [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MNGNNIIRNLMLEIPSAPGNLGKVTTAIGLAGGDIGEIETIKVGPNYTMRNITVQVEHEEQLTEILEVIRNLKEGIHLHT
VSDDVLAAHEGGKIRMKSKMPIRSLADLRRVYTPGVANVCELIKEEPQKANIYTGIGNSVAIVTDGTAILGLGDIGPVAG
MPVMEGKAALFDQLADISGVPILLDTNDPDEIVRTVKNIAPGFSGILLEDIGSPHCFEIEERLKNDLNIPVMHDDQHGTA
VVTLAAAISAAKSAGVDLKQAHVGQIGLGAAGVAICRMFMAYGVKRVVGTDKSLEAMARLENYGGHAAESIEELMESCDI
VVATTGVPGLINKKWVREGQVILALSNPKPEIEPEDALEAGAAYAADGRSVNNVLGFPGIFRGVLNAGADSISHPMLVAA
AEAIASCTRQGDLVPHPLNPEVHEIVAKAVERVALSEMGKING

Sequences:

>Translated_443_residues
MNGNNIIRNLMLEIPSAPGNLGKVTTAIGLAGGDIGEIETIKVGPNYTMRNITVQVEHEEQLTEILEVIRNLKEGIHLHT
VSDDVLAAHEGGKIRMKSKMPIRSLADLRRVYTPGVANVCELIKEEPQKANIYTGIGNSVAIVTDGTAILGLGDIGPVAG
MPVMEGKAALFDQLADISGVPILLDTNDPDEIVRTVKNIAPGFSGILLEDIGSPHCFEIEERLKNDLNIPVMHDDQHGTA
VVTLAAAISAAKSAGVDLKQAHVGQIGLGAAGVAICRMFMAYGVKRVVGTDKSLEAMARLENYGGHAAESIEELMESCDI
VVATTGVPGLINKKWVREGQVILALSNPKPEIEPEDALEAGAAYAADGRSVNNVLGFPGIFRGVLNAGADSISHPMLVAA
AEAIASCTRQGDLVPHPLNPEVHEIVAKAVERVALSEMGKING
>Mature_443_residues
MNGNNIIRNLMLEIPSAPGNLGKVTTAIGLAGGDIGEIETIKVGPNYTMRNITVQVEHEEQLTEILEVIRNLKEGIHLHT
VSDDVLAAHEGGKIRMKSKMPIRSLADLRRVYTPGVANVCELIKEEPQKANIYTGIGNSVAIVTDGTAILGLGDIGPVAG
MPVMEGKAALFDQLADISGVPILLDTNDPDEIVRTVKNIAPGFSGILLEDIGSPHCFEIEERLKNDLNIPVMHDDQHGTA
VVTLAAAISAAKSAGVDLKQAHVGQIGLGAAGVAICRMFMAYGVKRVVGTDKSLEAMARLENYGGHAAESIEELMESCDI
VVATTGVPGLINKKWVREGQVILALSNPKPEIEPEDALEAGAAYAADGRSVNNVLGFPGIFRGVLNAGADSISHPMLVAA
AEAIASCTRQGDLVPHPLNPEVHEIVAKAVERVALSEMGKING

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1788806, Length=384, Percent_Identity=36.9791666666667, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87081919, Length=443, Percent_Identity=26.1851015801354, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 46723; Mature: 46723

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGNNIIRNLMLEIPSAPGNLGKVTTAIGLAGGDIGEIETIKVGPNYTMRNITVQVEHEE
CCCHHHHHHHHHHCCCCCCCCCHHHHHHEECCCCCCCEEEEEECCCEEEEEEEEEEECHH
QLTEILEVIRNLKEGIHLHTVSDDVLAAHEGGKIRMKSKMPIRSLADLRRVYTPGVANVC
HHHHHHHHHHHHHCCCEEEEECCCCEEECCCCEEEEECCCCHHHHHHHHHHHCCCHHHHH
ELIKEEPQKANIYTGIGNSVAIVTDGTAILGLGDIGPVAGMPVMEGKAALFDQLADISGV
HHHHCCCCCCEEEECCCCEEEEEECCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCC
PILLDTNDPDEIVRTVKNIAPGFSGILLEDIGSPHCFEIEERLKNDLNIPVMHDDQHGTA
EEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCCCEEHHHHHHHCCCCCCEEECCCCCCH
VVTLAAAISAAKSAGVDLKQAHVGQIGLGAAGVAICRMFMAYGVKRVVGTDKSLEAMARL
HHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ENYGGHAAESIEELMESCDIVVATTGVPGLINKKWVREGQVILALSNPKPEIEPEDALEA
HHCCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHCCCCEEEEECCCCCCCCCHHHHHC
GAAYAADGRSVNNVLGFPGIFRGVLNAGADSISHPMLVAAAEAIASCTRQGDLVPHPLNP
CCCCCCCCCCCHHHCCCHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCC
EVHEIVAKAVERVALSEMGKING
HHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNGNNIIRNLMLEIPSAPGNLGKVTTAIGLAGGDIGEIETIKVGPNYTMRNITVQVEHEE
CCCHHHHHHHHHHCCCCCCCCCHHHHHHEECCCCCCCEEEEEECCCEEEEEEEEEEECHH
QLTEILEVIRNLKEGIHLHTVSDDVLAAHEGGKIRMKSKMPIRSLADLRRVYTPGVANVC
HHHHHHHHHHHHHCCCEEEEECCCCEEECCCCEEEEECCCCHHHHHHHHHHHCCCHHHHH
ELIKEEPQKANIYTGIGNSVAIVTDGTAILGLGDIGPVAGMPVMEGKAALFDQLADISGV
HHHHCCCCCCEEEECCCCEEEEEECCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCC
PILLDTNDPDEIVRTVKNIAPGFSGILLEDIGSPHCFEIEERLKNDLNIPVMHDDQHGTA
EEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCCCEEHHHHHHHCCCCCCEEECCCCCCH
VVTLAAAISAAKSAGVDLKQAHVGQIGLGAAGVAICRMFMAYGVKRVVGTDKSLEAMARL
HHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ENYGGHAAESIEELMESCDIVVATTGVPGLINKKWVREGQVILALSNPKPEIEPEDALEA
HHCCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHCCCCEEEEECCCCCCCCCHHHHHC
GAAYAADGRSVNNVLGFPGIFRGVLNAGADSISHPMLVAAAEAIASCTRQGDLVPHPLNP
CCCCCCCCCCCHHHCCCHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCC
EVHEIVAKAVERVALSEMGKING
HHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8501064; 10903309 [H]