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The map label for this gene is mleN [H]

Identifier: 52787815

GI number: 52787815

Start: 3964792

End: 3966189

Strand: Reverse

Name: mleN [H]

Synonym: BLi04138

Alternate gene names: 52787815

Gene position: 3966189-3964792 (Counterclockwise)

Preceding gene: 52787816

Following gene: 52787814

Centisome position: 93.93

GC content: 49.5

Gene sequence:

>1398_bases
TTGAAAACAGCACGGTTGCCATCAATGTTGGAAATCATTTGTGTATTAGGTGCATTTTTAGCGATAGTTTGTTCATTTAC
AGTCAAATTCGAGCTGCCGATTCAGCTGGCATTGTTTATTTCATGGTTCTTGGTGATTTTGCTCGGACTGCGCCTGGGGC
ACCGGTATGAAGATTTGCAAAAATCGATTACAAACGGAATTTCAAATGGACTTGAAGCGATATTGATCCTGATTGCCGTC
GGCGCTCTTATCGGCACATGGATAGCCGGCGGTGTTGTGCCGACTTTGATTTATTACGGGCTTGAGTTTATCCACCCGAG
CATATTCCTGCTTGCCACGCTCATTATTTGTTCGATCACATCTGTTGCCACAGGTACGTCTTGGGGGACTGTAGGAACGG
CCGGGATCGCCATGATGGCAATCGGGGAAGGGCTGGGGCTTCCGCTTCCGCTTGTAGCCGGAGCCGTGCTGTCCGGAGCC
TACTTTGGAGATAAATTATCGCCGCTTTCCGATAGTACGGTGCTTGCGTCATCCCTTTCAAAGGTCGAAGTGATTACCCA
TGTCAGAGCGATGCTGTACTTGTCGATTCCCGCCTATTTAATCACGGCGGTCCTGTTCACCATCACCGGCTTTATGTATG
GAAGCCGAAATGTTGATTTGGAAAAAGTTGAATTTTTGAAAGCTTCTTTGCAGAACACGTTTGATATTCACATTTGGATG
CTTGTTCCGGCTGTTGTCGTCATCGTTCTATTGGCGATGAGAAAGCCATCTGTGCCGACGATCGCGATCGGCGCACTTTT
GGGCGCGATCTGGGCTGCGGTCTTCCAAGGCATGGACTTTGCGGATGCGATCGGCACAGCATACAACGGCTTTTCCATTG
ACACCGGGATCAAATTTATGGACGAGCTCTTAAATCGCGGAGGAATAGAGGGCATGCTCGGTTCGGTCGTCGTCATCATT
CTCGGTCTGGGCTTTGGCGGCCTCTTGGAGCACTTAGGCGTTCTAAAAGTGATCGTCTCCAAGTTCCAGCAAAAGCTGAC
TTCTGCCGGCAACGTCACATTCTCGACGATTATCGTCGCCTTCTTGGCAAATGTGTTCGGCTGCGCGATGTATGTCTCGC
TCATTTTAACACCGAAAATTATGGAAAAAAGCTATGACAAGCTCGGAATTGACCGGAGGGTGCTGTCCAGAAACGCGGAA
GTCGGCGGTACGATGACGTCGGGCATGGTGCCGTGGTCGGACAACGGCATATATATGGCGGGGATTCTCGGCGTCTCGAC
CTTCTCTTATGTGCCGTTCATGTGGCTGAGCTTTGTTTCAATCGGCCTTGCGATTTTGTACGGATACACAGGTAAATTTA
TTTGGTACACCGATAAAAAAGACTCGCCGGTTCAGTAG

Upstream 100 bases:

>100_bases
CTGTTCGTTGTTAGTTAAATTTTTAAGAAAAAATTGAAGCAGATTGCTATTTTTATGTAGTTTATTTTGAAAGCGTTTCA
ATAATTAAGGGGGATTTAGG

Downstream 100 bases:

>100_bases
CCGATTTTAAAACAATTGATGGAGGTATTCTGTTATGAATGGCAACAATATTATTCGGAATTTGATGCTGGAAATCCCTT
CAGCACCCGGAAACCTTGGA

Product: MleN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MKTARLPSMLEIICVLGAFLAIVCSFTVKFELPIQLALFISWFLVILLGLRLGHRYEDLQKSITNGISNGLEAILILIAV
GALIGTWIAGGVVPTLIYYGLEFIHPSIFLLATLIICSITSVATGTSWGTVGTAGIAMMAIGEGLGLPLPLVAGAVLSGA
YFGDKLSPLSDSTVLASSLSKVEVITHVRAMLYLSIPAYLITAVLFTITGFMYGSRNVDLEKVEFLKASLQNTFDIHIWM
LVPAVVVIVLLAMRKPSVPTIAIGALLGAIWAAVFQGMDFADAIGTAYNGFSIDTGIKFMDELLNRGGIEGMLGSVVVII
LGLGFGGLLEHLGVLKVIVSKFQQKLTSAGNVTFSTIIVAFLANVFGCAMYVSLILTPKIMEKSYDKLGIDRRVLSRNAE
VGGTMTSGMVPWSDNGIYMAGILGVSTFSYVPFMWLSFVSIGLAILYGYTGKFIWYTDKKDSPVQ

Sequences:

>Translated_465_residues
MKTARLPSMLEIICVLGAFLAIVCSFTVKFELPIQLALFISWFLVILLGLRLGHRYEDLQKSITNGISNGLEAILILIAV
GALIGTWIAGGVVPTLIYYGLEFIHPSIFLLATLIICSITSVATGTSWGTVGTAGIAMMAIGEGLGLPLPLVAGAVLSGA
YFGDKLSPLSDSTVLASSLSKVEVITHVRAMLYLSIPAYLITAVLFTITGFMYGSRNVDLEKVEFLKASLQNTFDIHIWM
LVPAVVVIVLLAMRKPSVPTIAIGALLGAIWAAVFQGMDFADAIGTAYNGFSIDTGIKFMDELLNRGGIEGMLGSVVVII
LGLGFGGLLEHLGVLKVIVSKFQQKLTSAGNVTFSTIIVAFLANVFGCAMYVSLILTPKIMEKSYDKLGIDRRVLSRNAE
VGGTMTSGMVPWSDNGIYMAGILGVSTFSYVPFMWLSFVSIGLAILYGYTGKFIWYTDKKDSPVQ
>Mature_465_residues
MKTARLPSMLEIICVLGAFLAIVCSFTVKFELPIQLALFISWFLVILLGLRLGHRYEDLQKSITNGISNGLEAILILIAV
GALIGTWIAGGVVPTLIYYGLEFIHPSIFLLATLIICSITSVATGTSWGTVGTAGIAMMAIGEGLGLPLPLVAGAVLSGA
YFGDKLSPLSDSTVLASSLSKVEVITHVRAMLYLSIPAYLITAVLFTITGFMYGSRNVDLEKVEFLKASLQNTFDIHIWM
LVPAVVVIVLLAMRKPSVPTIAIGALLGAIWAAVFQGMDFADAIGTAYNGFSIDTGIKFMDELLNRGGIEGMLGSVVVII
LGLGFGGLLEHLGVLKVIVSKFQQKLTSAGNVTFSTIIVAFLANVFGCAMYVSLILTPKIMEKSYDKLGIDRRVLSRNAE
VGGTMTSGMVPWSDNGIYMAGILGVSTFSYVPFMWLSFVSIGLAILYGYTGKFIWYTDKKDSPVQ

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 49834; Mature: 49834

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTARLPSMLEIICVLGAFLAIVCSFTVKFELPIQLALFISWFLVILLGLRLGHRYEDLQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KSITNGISNGLEAILILIAVGALIGTWIAGGVVPTLIYYGLEFIHPSIFLLATLIICSIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVATGTSWGTVGTAGIAMMAIGEGLGLPLPLVAGAVLSGAYFGDKLSPLSDSTVLASSLS
HHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
KVEVITHVRAMLYLSIPAYLITAVLFTITGFMYGSRNVDLEKVEFLKASLQNTFDIHIWM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHH
LVPAVVVIVLLAMRKPSVPTIAIGALLGAIWAAVFQGMDFADAIGTAYNGFSIDTGIKFM
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHH
DELLNRGGIEGMLGSVVVIILGLGFGGLLEHLGVLKVIVSKFQQKLTSAGNVTFSTIIVA
HHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FLANVFGCAMYVSLILTPKIMEKSYDKLGIDRRVLSRNAEVGGTMTSGMVPWSDNGIYMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHCCCCCCCCCCEEEE
GILGVSTFSYVPFMWLSFVSIGLAILYGYTGKFIWYTDKKDSPVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MKTARLPSMLEIICVLGAFLAIVCSFTVKFELPIQLALFISWFLVILLGLRLGHRYEDLQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KSITNGISNGLEAILILIAVGALIGTWIAGGVVPTLIYYGLEFIHPSIFLLATLIICSIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVATGTSWGTVGTAGIAMMAIGEGLGLPLPLVAGAVLSGAYFGDKLSPLSDSTVLASSLS
HHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
KVEVITHVRAMLYLSIPAYLITAVLFTITGFMYGSRNVDLEKVEFLKASLQNTFDIHIWM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHH
LVPAVVVIVLLAMRKPSVPTIAIGALLGAIWAAVFQGMDFADAIGTAYNGFSIDTGIKFM
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHH
DELLNRGGIEGMLGSVVVIILGLGFGGLLEHLGVLKVIVSKFQQKLTSAGNVTFSTIIVA
HHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FLANVFGCAMYVSLILTPKIMEKSYDKLGIDRRVLSRNAEVGGTMTSGMVPWSDNGIYMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHCCCCCCCCCCEEEE
GILGVSTFSYVPFMWLSFVSIGLAILYGYTGKFIWYTDKKDSPVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]