Click here to switch to the map view.

The map label for this gene is hisH

Identifier: 52787417

GI number: 52787417

Start: 3558119

End: 3558757

Strand: Reverse

Name: hisH

Synonym: BLi03734

Alternate gene names: 52787417

Gene position: 3558757-3558119 (Counterclockwise)

Preceding gene: 52787418

Following gene: 52787416

Centisome position: 84.28

GC content: 48.83

Gene sequence:

>639_bases
ATGATCGGTGTCATAGATTACGGAATGGGAAACCTGTACAGCGTTTCTAAAGCGCTTGAACGGATTGATGCCCCTTATTT
TGTGTCCGAGCATCCGGATGAACTGAAGAGAGCGGACAGCTATATCCTTCCGGGAGTCGGCGCTTTTCGGGATGCAATGG
AGATTTTAACCGAGAACGGCCTGAAAACATTTATTCAAGCTGCCGCAAACGAAGGAAAACCTCTTCTCGGCATATGCCTC
GGCATGCAGCTTTTGTTTGAAGAAAGCGAAGAACACGGGGCGTCAGAAGGGCTCGGCCTGTTGAAAGGAAAAGTCGTTAA
ACTGAAAGACTGTGATCAAGCGGGAAATCGCTTAAAAGTGCCTCACATGGGCTGGAATCTTCTCAAAGTCCACCGCGACT
CGCCGCTTTTGCCAAAAGCGAAAGAAGGATTCGCGTATTTCGTTCATTCGTATTATGTCAGCGGAATCGAAGAGGAAGCT
TTGCTTGCAAGCGCCGAATACGGTGTGTGTGTACCCGCTGTCGTCGGTCTTGGCAATGTATACGGGGCCCAGTTCCATCC
GGAAAAGAGCAGTACGGTCGGCATGTTGATCTTAGAGCGGTTCAAGCAGTTTACACAAGAACAGAAGGTGAAAAAATGA

Upstream 100 bases:

>100_bases
GATCGAGGCCATTTTTAAAGCGCTGGGACGTGCGCTTGATGAAGCGGCATTGATCGATCCGCGGGTCAAGGGAATTCCAT
CAACGAAAGGAATGCTGTAA

Downstream 100 bases:

>100_bases
GCGAGTTTACTGTATATCCTGCCATTGACATAAGAAACGGCAAGTGCGTCAGGCTCATCCAGGGAGATTACGAAAAAGAA
ACGATTTACGGTCATTCCCC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MIGVIDYGMGNLYSVSKALERIDAPYFVSEHPDELKRADSYILPGVGAFRDAMEILTENGLKTFIQAAANEGKPLLGICL
GMQLLFEESEEHGASEGLGLLKGKVVKLKDCDQAGNRLKVPHMGWNLLKVHRDSPLLPKAKEGFAYFVHSYYVSGIEEEA
LLASAEYGVCVPAVVGLGNVYGAQFHPEKSSTVGMLILERFKQFTQEQKVKK

Sequences:

>Translated_212_residues
MIGVIDYGMGNLYSVSKALERIDAPYFVSEHPDELKRADSYILPGVGAFRDAMEILTENGLKTFIQAAANEGKPLLGICL
GMQLLFEESEEHGASEGLGLLKGKVVKLKDCDQAGNRLKVPHMGWNLLKVHRDSPLLPKAKEGFAYFVHSYYVSGIEEEA
LLASAEYGVCVPAVVGLGNVYGAQFHPEKSSTVGMLILERFKQFTQEQKVKK
>Mature_212_residues
MIGVIDYGMGNLYSVSKALERIDAPYFVSEHPDELKRADSYILPGVGAFRDAMEILTENGLKTFIQAAANEGKPLLGICL
GMQLLFEESEEHGASEGLGLLKGKVVKLKDCDQAGNRLKVPHMGWNLLKVHRDSPLLPKAKEGFAYFVHSYYVSGIEEEA
LLASAEYGVCVPAVVGLGNVYGAQFHPEKSSTVGMLILERFKQFTQEQKVKK

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=199, Percent_Identity=38.1909547738693, Blast_Score=115, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319725, Length=219, Percent_Identity=30.1369863013699, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_BACLD (Q65EG1)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080820.1
- RefSeq:   YP_093246.1
- ProteinModelPortal:   Q65EG1
- SMR:   Q65EG1
- STRING:   Q65EG1
- EnsemblBacteria:   EBBACT00000055609
- EnsemblBacteria:   EBBACT00000062523
- GeneID:   3029111
- GeneID:   3100884
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03734
- KEGG:   bli:BL03410
- NMPDR:   fig|279010.5.peg.3799
- eggNOG:   COG0118
- GeneTree:   EBGT00050000000583
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- ProtClustDB:   PRK13141
- BioCyc:   BLIC279010-1:BLI03734-MONOMER
- BioCyc:   BLIC279010:BL03410-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23308; Mature: 23308

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 186-186 ACT_SITE 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGVIDYGMGNLYSVSKALERIDAPYFVSEHPDELKRADSYILPGVGAFRDAMEILTENG
CEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCH
LKTFIQAAANEGKPLLGICLGMQLLFEESEEHGASEGLGLLKGKVVKLKDCDQAGNRLKV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHCCCHHHHCCEEEEECHHHCCCEEEC
PHMGWNLLKVHRDSPLLPKAKEGFAYFVHSYYVSGIEEEALLASAEYGVCVPAVVGLGNV
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHCCHH
YGAQFHPEKSSTVGMLILERFKQFTQEQKVKK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIGVIDYGMGNLYSVSKALERIDAPYFVSEHPDELKRADSYILPGVGAFRDAMEILTENG
CEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCH
LKTFIQAAANEGKPLLGICLGMQLLFEESEEHGASEGLGLLKGKVVKLKDCDQAGNRLKV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHCCCHHHHCCEEEEECHHHCCCEEEC
PHMGWNLLKVHRDSPLLPKAKEGFAYFVHSYYVSGIEEEALLASAEYGVCVPAVVGLGNV
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHCCHH
YGAQFHPEKSSTVGMLILERFKQFTQEQKVKK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA