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The map label for this gene is hisA

Identifier: 52787416

GI number: 52787416

Start: 3557385

End: 3558122

Strand: Reverse

Name: hisA

Synonym: BLi03733

Alternate gene names: 52787416

Gene position: 3558122-3557385 (Counterclockwise)

Preceding gene: 52787417

Following gene: 52787415

Centisome position: 84.26

GC content: 51.76

Gene sequence:

>738_bases
ATGAGCGAGTTTACTGTATATCCTGCCATTGACATAAGAAACGGCAAGTGCGTCAGGCTCATCCAGGGAGATTACGAAAA
AGAAACGATTTACGGTCATTCCCCTCTGGAGATGGCCTCACGGTTTGCTGAAGAGGGGGCAAGCTGGATTCATCTTGTCG
ATTTGGACGGCGCAAGAGAAGGCAAAAAAGTGAACGGCGGCCACGTCATAAGCATTGCTGATAAGCTTGACGTTAAAGTG
CAAGTCGGCGGCGGCATCAGGACCGAAAAAGACGTGTATGACTACTTGTCGCAAGGCGTTGAGAGGGTGATCCTAGGCAG
CTCTGCCGTATCAAATACCGAGTTTGTCAAAAAAATGCTGAAGGCCTACGGAGGGCACATCGCAATCGGTCTTGACGCAA
GGGACGGATTTGTTTCGACTGAAGGGTGGCTTGAGACCTCTTCCGTTAGAGCGGAAGAGCTTGGCCGCGAGCTTGCAAAT
GAAGGTGCAGAAGTTTTTATCTTCACTGACATTGCGACTGACGGAATGCTGTCAGGACCTAATATCAACAGTACAGTGGA
GCTTGCCAGGGCAACCGGCAAACAAGTCATTGCCTCCGGCGGCGTCAGTTCTCTCGCCGATCTTGAGGCGCTCGCTTCCC
GCGCTTCGGAAGGCGTCTCTGGCGCTATTGTCGGAAAAGCTCTTTATACCAACCAATTTACCGTGGCTGAAGCGCTTGAA
AGGGTGGCGGCAAAGTGA

Upstream 100 bases:

>100_bases
ATGTATACGGGGCCCAGTTCCATCCGGAAAAGAGCAGTACGGTCGGCATGTTGATCTTAGAGCGGTTCAAGCAGTTTACA
CAAGAACAGAAGGTGAAAAA

Downstream 100 bases:

>100_bases
TCACAAAACGAATCATCCCGTGTTTGGACGTAAAAGATGGACGCGTTGTCAAAGGTGTACAATTTGTTGAATTGAAAGAT
GCCGGCGACCCGGTTGAACT

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSEFTVYPAIDIRNGKCVRLIQGDYEKETIYGHSPLEMASRFAEEGASWIHLVDLDGAREGKKVNGGHVISIADKLDVKV
QVGGGIRTEKDVYDYLSQGVERVILGSSAVSNTEFVKKMLKAYGGHIAIGLDARDGFVSTEGWLETSSVRAEELGRELAN
EGAEVFIFTDIATDGMLSGPNINSTVELARATGKQVIASGGVSSLADLEALASRASEGVSGAIVGKALYTNQFTVAEALE
RVAAK

Sequences:

>Translated_245_residues
MSEFTVYPAIDIRNGKCVRLIQGDYEKETIYGHSPLEMASRFAEEGASWIHLVDLDGAREGKKVNGGHVISIADKLDVKV
QVGGGIRTEKDVYDYLSQGVERVILGSSAVSNTEFVKKMLKAYGGHIAIGLDARDGFVSTEGWLETSSVRAEELGRELAN
EGAEVFIFTDIATDGMLSGPNINSTVELARATGKQVIASGGVSSLADLEALASRASEGVSGAIVGKALYTNQFTVAEALE
RVAAK
>Mature_244_residues
SEFTVYPAIDIRNGKCVRLIQGDYEKETIYGHSPLEMASRFAEEGASWIHLVDLDGAREGKKVNGGHVISIADKLDVKVQ
VGGGIRTEKDVYDYLSQGVERVILGSSAVSNTEFVKKMLKAYGGHIAIGLDARDGFVSTEGWLETSSVRAEELGRELANE
GAEVFIFTDIATDGMLSGPNINSTVELARATGKQVIASGGVSSLADLEALASRASEGVSGAIVGKALYTNQFTVAEALER
VAAK

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=241, Percent_Identity=38.1742738589212, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1788336, Length=243, Percent_Identity=21.3991769547325, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_BACLD (Q65EG2)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080819.1
- RefSeq:   YP_093245.1
- ProteinModelPortal:   Q65EG2
- SMR:   Q65EG2
- STRING:   Q65EG2
- EnsemblBacteria:   EBBACT00000054959
- EnsemblBacteria:   EBBACT00000061305
- GeneID:   3029544
- GeneID:   3100883
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03733
- KEGG:   bli:BL03411
- NMPDR:   fig|279010.5.peg.3798
- eggNOG:   COG0106
- GeneTree:   EBGT00050000001074
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- BioCyc:   BLIC279010-1:BLI03733-MONOMER
- BioCyc:   BLIC279010:BL03411-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26060; Mature: 25928

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFTVYPAIDIRNGKCVRLIQGDYEKETIYGHSPLEMASRFAEEGASWIHLVDLDGARE
CCCEEEEEEEEECCCCEEEEECCCCCCCEEECCCHHHHHHHHHHCCCCEEEEEECCCCCC
GKKVNGGHVISIADKLDVKVQVGGGIRTEKDVYDYLSQGVERVILGSSAVSNTEFVKKML
CCCCCCCEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHEEHHCCCCCCCHHHHHHHH
KAYGGHIAIGLDARDGFVSTEGWLETSSVRAEELGRELANEGAEVFIFTDIATDGMLSGP
HHCCCEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
NINSTVELARATGKQVIASGGVSSLADLEALASRASEGVSGAIVGKALYTNQFTVAEALE
CCCHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCHHHHHHH
RVAAK
HHHCC
>Mature Secondary Structure 
SEFTVYPAIDIRNGKCVRLIQGDYEKETIYGHSPLEMASRFAEEGASWIHLVDLDGARE
CCEEEEEEEEECCCCEEEEECCCCCCCEEECCCHHHHHHHHHHCCCCEEEEEECCCCCC
GKKVNGGHVISIADKLDVKVQVGGGIRTEKDVYDYLSQGVERVILGSSAVSNTEFVKKML
CCCCCCCEEEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHEEHHCCCCCCCHHHHHHHH
KAYGGHIAIGLDARDGFVSTEGWLETSSVRAEELGRELANEGAEVFIFTDIATDGMLSGP
HHCCCEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
NINSTVELARATGKQVIASGGVSSLADLEALASRASEGVSGAIVGKALYTNQFTVAEALE
CCCHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCHHHHHHH
RVAAK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA