Click here to switch to the map view.

The map label for this gene is yvfD [H]

Identifier: 52787366

GI number: 52787366

Start: 3509062

End: 3509688

Strand: Reverse

Name: yvfD [H]

Synonym: BLi03682

Alternate gene names: 52787366

Gene position: 3509688-3509062 (Counterclockwise)

Preceding gene: 52787367

Following gene: 52787365

Centisome position: 83.12

GC content: 56.78

Gene sequence:

>627_bases
ATGCAAAACGTGGTGATCATCGGAGCCGGCGGCCATGGGAAAGTCGTTCGGGAACTTGTAAAAGAGCGGCCGGATACGGA
GCTTGCCGGTATTCTTGATGACCGGTATGCGGAGCTTCATGTTGAGAACGGTCTGTATCGAGGGCCTTCAGCTGCCGCAG
AAGAACTTGCGCGGCTTCATCCGGACGCGAAGTTCGTCCTGGCTGTCGGCCAAAACAGCATCAGACAGCAGCTGTATGAA
CGCATTGGTCTTCCGCTTGACCGGTATGCGGTTTTGATTCATCCCTCTGCTGTTGTCAGCGGTTCGGCCCGGATTCAAAA
CGGCGCCGTTGTTATGGCATCGAGCGTCATCCAAGCGGATGCAGACGTCGGCATCCACGCGATTGTCAACACAGGTGCGA
TCGTCGAACACGACAATCGGATCGGCGATTACGTTCATCTTTCGCCCGGAACGGTGTTAACCGGCGGCGTGACAGTTATG
GAAGGCGCTCATCTCGGCGCGGGAACGGCGGTCATTCCCGGAAAGACAGTCGGACGCTGGAGCGTGACGGGAGCGGGGGC
AGCCGTGATTCACGACATTCCTGATAATTGCACCGCAGTCGGAGTCCCTGCAAGAATGATCAAATAA

Upstream 100 bases:

>100_bases
AAATCCTTTTGTTGACTGTCGTAAAGGTTTTGAAGTCGGAAGGCGTCAGCCAGGACCGGCATGTGACAGCAGAAAAATTT
ACAGGGAGAAGGAATGCCTG

Downstream 100 bases:

>100_bases
CAGCAGTAAAAAGGGTGAAAAACATGAGTCAGAATAAGCGAATTTATTTATCACCGCCGCACATGAGCGGAGACGAGGAG
CGCTATGTAGCCGAAGCGTT

Product: YvfD

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MQNVVIIGAGGHGKVVRELVKERPDTELAGILDDRYAELHVENGLYRGPSAAAEELARLHPDAKFVLAVGQNSIRQQLYE
RIGLPLDRYAVLIHPSAVVSGSARIQNGAVVMASSVIQADADVGIHAIVNTGAIVEHDNRIGDYVHLSPGTVLTGGVTVM
EGAHLGAGTAVIPGKTVGRWSVTGAGAAVIHDIPDNCTAVGVPARMIK

Sequences:

>Translated_208_residues
MQNVVIIGAGGHGKVVRELVKERPDTELAGILDDRYAELHVENGLYRGPSAAAEELARLHPDAKFVLAVGQNSIRQQLYE
RIGLPLDRYAVLIHPSAVVSGSARIQNGAVVMASSVIQADADVGIHAIVNTGAIVEHDNRIGDYVHLSPGTVLTGGVTVM
EGAHLGAGTAVIPGKTVGRWSVTGAGAAVIHDIPDNCTAVGVPARMIK
>Mature_208_residues
MQNVVIIGAGGHGKVVRELVKERPDTELAGILDDRYAELHVENGLYRGPSAAAEELARLHPDAKFVLAVGQNSIRQQLYE
RIGLPLDRYAVLIHPSAVVSGSARIQNGAVVMASSVIQADADVGIHAIVNTGAIVEHDNRIGDYVHLSPGTVLTGGVTVM
EGAHLGAGTAVIPGKTVGRWSVTGAGAAVIHDIPDNCTAVGVPARMIK

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR013817
- InterPro:   IPR020019
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 21696; Mature: 21696

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNVVIIGAGGHGKVVRELVKERPDTELAGILDDRYAELHVENGLYRGPSAAAEELARLH
CCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHC
PDAKFVLAVGQNSIRQQLYERIGLPLDRYAVLIHPSAVVSGSARIQNGAVVMASSVIQAD
CCCEEEEEECCHHHHHHHHHHHCCCHHHEEEEEECHHHCCCCCEECCCEEEEEEHHHHCC
ADVGIHAIVNTGAIVEHDNRIGDYVHLSPGTVLTGGVTVMEGAHLGAGTAVIPGKTVGRW
CCCCEEEEEECCEEEECCCCCCCEEEECCCCEEECCEEEECCCCCCCCEEECCCCCCCEE
SVTGAGAAVIHDIPDNCTAVGVPARMIK
EECCCCEEEEEECCCCCEEECCCHHHCC
>Mature Secondary Structure
MQNVVIIGAGGHGKVVRELVKERPDTELAGILDDRYAELHVENGLYRGPSAAAEELARLH
CCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHC
PDAKFVLAVGQNSIRQQLYERIGLPLDRYAVLIHPSAVVSGSARIQNGAVVMASSVIQAD
CCCEEEEEECCHHHHHHHHHHHCCCHHHEEEEEECHHHCCCCCEECCCEEEEEEHHHHCC
ADVGIHAIVNTGAIVEHDNRIGDYVHLSPGTVLTGGVTVMEGAHLGAGTAVIPGKTVGRW
CCCCEEEEEECCEEEECCCCCCCEEEECCCCEEECCEEEECCCCCCCCEEECCCCCCCEE
SVTGAGAAVIHDIPDNCTAVGVPARMIK
EECCCCEEEEEECCCCCEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]