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The map label for this gene is cssS [H]

Identifier: 52787174

GI number: 52787174

Start: 3324155

End: 3325501

Strand: Direct

Name: cssS [H]

Synonym: BLi03483

Alternate gene names: 52787174

Gene position: 3324155-3325501 (Clockwise)

Preceding gene: 52787173

Following gene: 52787176

Centisome position: 78.72

GC content: 45.21

Gene sequence:

>1347_bases
ATGAAGAACAGGTCGCTCGCCTTTCAAATTTGGAGTGTCATTTGCGGCATCCTGTTAACGGTCTCGATTTTGCTGACGTT
TCTATTCTCGACGACACTGCAGAACTTTTTCACTGATGAAATTTATACAAACATTGAAAATGAGCAGCATGTTCTGACGG
AATACGGATTTTCCGATTCACCGGAAAGAGGCTTCGGCGGAAGCAGCGGCTCGCGAAGCACAAACCGCCACGTTCAGCAC
GTCCTGCTGCCTGAGAAAGGAACCGTCTATCAGAGCCCGCAGATTCTCCCCGATTCATTTATCGAGAAGGTGCAGAAGCT
CGGGAAAAGGCAGGACGAGTCATCGAAAAGGTATTCGGAGAATGTCAACGGAGAGCACGTTTTCTTTGTGCTCAAAAAAA
TCCAGACCCAGGACCAGACGTTTCTGTTGTTATCCTATGCGCTTGATTCATACAGGGACGATTTGTCTCATACGCTGTTT
AAGCAGCTGCTGTTTATTTTGATCGTCGTCATTTTGCTGAGCTGGATTCCTTCTATTTGGCTGGCGAAATATTTATCAAG
ACCGCTCGTCACGCTTGAAAAGCACGTCAAACAAATCTCTAAACAGGATTGGGATGATCCCGTGATTGTCGACAGGGAAG
ACGAAATCGGCAAGCTGGGGCACACCATTGAAGACATGCGGCAAAAGCTCATCCAGAAAGATGAGACAGAACGCACGCTC
CTGCAAAACATCTCCCACGACCTGAAAACGCCGGTCATGGTCATCCGCGGGTATACCCAGTCGATCAAAGACGGCATCTA
CCCTAAAGGAGATTTGGAAAACACGATTGAAGTCATTGAAGGCGAAGCTGAAAAACTTGAAAAGAAAATCAAGGATCTTC
TTTATCTGACAAAGCTTGATTATTTGCTGAGACAAGAAAGACAGTACGGAACTTTTGATATACAAGAGGTCATTAAAGAA
GTTGTTGAACGCTTGAGATGGTCCAAAAAAGAATTGAACTGGAAGATTGATCTTGAAGAGGAAACAATGTTGACGGGCGA
TCAAGAACAATGGAGCAAGCTGTTGGAAAACGTTCTTGAAAACCAGATCCGCTATGCGGAGACATTCATTGACATCAGCG
TCAGGTCGACGAAAGAACATACGTTGATCACCATTAAAAATGACGGACCGCACATTGAAGAAGAGATGCTTTCCAGCCTG
TATGAGCCTTTTAATAAAGGGAAGAAAGGCGAATTCGGCATTGGACTCAGCATCGTTAAACGAATCTTAACGCTCCACCA
TGCTGACATGACCATTCGGAATGATCAAACCGGTGTGTGCTACTCAATATCCGTTCCGAGAAATTAG

Upstream 100 bases:

>100_bases
CCGACCGCGTCGTCGACGATCTTGTCAGAAGGCTGCGCAAGAAAATGCCCGAACTGAAAGTGGAAACCATCTACGGGTTT
GGCTACAGGATGTTGAAATC

Downstream 100 bases:

>100_bases
ACTGTAGACGCTGCAGTCTTTTTTCATATAAACGAATAGCCGTTGAAATCCCGGTAGCTCGGCTTCACTGTGTTTTTTGG
CTTTTTCATCCATTTCTTTT

Product: CssS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MKNRSLAFQIWSVICGILLTVSILLTFLFSTTLQNFFTDEIYTNIENEQHVLTEYGFSDSPERGFGGSSGSRSTNRHVQH
VLLPEKGTVYQSPQILPDSFIEKVQKLGKRQDESSKRYSENVNGEHVFFVLKKIQTQDQTFLLLSYALDSYRDDLSHTLF
KQLLFILIVVILLSWIPSIWLAKYLSRPLVTLEKHVKQISKQDWDDPVIVDREDEIGKLGHTIEDMRQKLIQKDETERTL
LQNISHDLKTPVMVIRGYTQSIKDGIYPKGDLENTIEVIEGEAEKLEKKIKDLLYLTKLDYLLRQERQYGTFDIQEVIKE
VVERLRWSKKELNWKIDLEEETMLTGDQEQWSKLLENVLENQIRYAETFIDISVRSTKEHTLITIKNDGPHIEEEMLSSL
YEPFNKGKKGEFGIGLSIVKRILTLHHADMTIRNDQTGVCYSISVPRN

Sequences:

>Translated_448_residues
MKNRSLAFQIWSVICGILLTVSILLTFLFSTTLQNFFTDEIYTNIENEQHVLTEYGFSDSPERGFGGSSGSRSTNRHVQH
VLLPEKGTVYQSPQILPDSFIEKVQKLGKRQDESSKRYSENVNGEHVFFVLKKIQTQDQTFLLLSYALDSYRDDLSHTLF
KQLLFILIVVILLSWIPSIWLAKYLSRPLVTLEKHVKQISKQDWDDPVIVDREDEIGKLGHTIEDMRQKLIQKDETERTL
LQNISHDLKTPVMVIRGYTQSIKDGIYPKGDLENTIEVIEGEAEKLEKKIKDLLYLTKLDYLLRQERQYGTFDIQEVIKE
VVERLRWSKKELNWKIDLEEETMLTGDQEQWSKLLENVLENQIRYAETFIDISVRSTKEHTLITIKNDGPHIEEEMLSSL
YEPFNKGKKGEFGIGLSIVKRILTLHHADMTIRNDQTGVCYSISVPRN
>Mature_448_residues
MKNRSLAFQIWSVICGILLTVSILLTFLFSTTLQNFFTDEIYTNIENEQHVLTEYGFSDSPERGFGGSSGSRSTNRHVQH
VLLPEKGTVYQSPQILPDSFIEKVQKLGKRQDESSKRYSENVNGEHVFFVLKKIQTQDQTFLLLSYALDSYRDDLSHTLF
KQLLFILIVVILLSWIPSIWLAKYLSRPLVTLEKHVKQISKQDWDDPVIVDREDEIGKLGHTIEDMRQKLIQKDETERTL
LQNISHDLKTPVMVIRGYTQSIKDGIYPKGDLENTIEVIEGEAEKLEKKIKDLLYLTKLDYLLRQERQYGTFDIQEVIKE
VVERLRWSKKELNWKIDLEEETMLTGDQEQWSKLLENVLENQIRYAETFIDISVRSTKEHTLITIKNDGPHIEEEMLSSL
YEPFNKGKKGEFGIGLSIVKRILTLHHADMTIRNDQTGVCYSISVPRN

Specific function: Member of the two-component regulatory system CssS/CssR required to control the cellular response to secretion stress. Required for the transcription of htrA. Could detect misfolded proteins at the membrane-cell wall interface and then activate CssR by ph

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=276, Percent_Identity=25, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787374, Length=283, Percent_Identity=24.3816254416961, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789808, Length=303, Percent_Identity=24.4224422442244, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786783, Length=322, Percent_Identity=23.2919254658385, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790861, Length=284, Percent_Identity=23.5915492957746, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786600, Length=230, Percent_Identity=23.4782608695652, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790551, Length=267, Percent_Identity=22.0973782771536, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693157, Length=250, Percent_Identity=22.8, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790346, Length=221, Percent_Identity=23.5294117647059, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786912, Length=232, Percent_Identity=24.5689655172414, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082128, Length=299, Percent_Identity=22.4080267558528, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1788279, Length=308, Percent_Identity=23.3766233766234, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52102; Mature: 52102

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRSLAFQIWSVICGILLTVSILLTFLFSTTLQNFFTDEIYTNIENEQHVLTEYGFSDS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
PERGFGGSSGSRSTNRHVQHVLLPEKGTVYQSPQILPDSFIEKVQKLGKRQDESSKRYSE
CCCCCCCCCCCCCHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHC
NVNGEHVFFVLKKIQTQDQTFLLLSYALDSYRDDLSHTLFKQLLFILIVVILLSWIPSIW
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKYLSRPLVTLEKHVKQISKQDWDDPVIVDREDEIGKLGHTIEDMRQKLIQKDETERTL
HHHHHHCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHCCHHHHHHHHHHCCCCHHHHH
LQNISHDLKTPVMVIRGYTQSIKDGIYPKGDLENTIEVIEGEAEKLEKKIKDLLYLTKLD
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
YLLRQERQYGTFDIQEVIKEVVERLRWSKKELNWKIDLEEETMLTGDQEQWSKLLENVLE
HHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHCCEEECCCHHHHCCCCHHHHHHHHHHHHH
NQIRYAETFIDISVRSTKEHTLITIKNDGPHIEEEMLSSLYEPFNKGKKGEFGIGLSIVK
HHHHHHHHHHEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
RILTLHHADMTIRNDQTGVCYSISVPRN
HHHHHHHCCCEEECCCCCEEEEECCCCC
>Mature Secondary Structure
MKNRSLAFQIWSVICGILLTVSILLTFLFSTTLQNFFTDEIYTNIENEQHVLTEYGFSDS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
PERGFGGSSGSRSTNRHVQHVLLPEKGTVYQSPQILPDSFIEKVQKLGKRQDESSKRYSE
CCCCCCCCCCCCCHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHC
NVNGEHVFFVLKKIQTQDQTFLLLSYALDSYRDDLSHTLFKQLLFILIVVILLSWIPSIW
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKYLSRPLVTLEKHVKQISKQDWDDPVIVDREDEIGKLGHTIEDMRQKLIQKDETERTL
HHHHHHCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHCCHHHHHHHHHHCCCCHHHHH
LQNISHDLKTPVMVIRGYTQSIKDGIYPKGDLENTIEVIEGEAEKLEKKIKDLLYLTKLD
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
YLLRQERQYGTFDIQEVIKEVVERLRWSKKELNWKIDLEEETMLTGDQEQWSKLLENVLE
HHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHCCEEECCCHHHHCCCCHHHHHHHHHHHHH
NQIRYAETFIDISVRSTKEHTLITIKNDGPHIEEEMLSSLYEPFNKGKKGEFGIGLSIVK
HHHHHHHHHHEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
RILTLHHADMTIRNDQTGVCYSISVPRN
HHHHHHHCCCEEECCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353931; 9639930; 9384377; 11555295 [H]