Click here to switch to the map view.

The map label for this gene is polA [H]

Identifier: 52786786

GI number: 52786786

Start: 2940321

End: 2942960

Strand: Reverse

Name: polA [H]

Synonym: BLi03057

Alternate gene names: 52786786

Gene position: 2942960-2940321 (Counterclockwise)

Preceding gene: 52786787

Following gene: 52786785

Centisome position: 69.69

GC content: 47.84

Gene sequence:

>2640_bases
ATGACTGAAAGAAAAAAATTAGTATTGGTTGACGGAAACAGCCTGGCTTACCGTGCGTTTTTCGCCCTGCCTCTTCTCAG
CAATGAAAAAGGGATACATACAAACGCGGTGTACGGTTTTACCACGATCTTAATGAAGATGCTTGAAGAGGAAAAACCGA
CCCACATGCTCGTCGCTTTTGATGCGGGGAAAACGACATTCAGACATAAAACGTTTAAAGAATATAAAGGCGGCAGACAA
AAAACGCCGCCGGAGCTGTCAGAGCAATTGCCGTTTATCCGCGAGCTGCTCGACGCATATAGAATCTCAAGGTATGAGCT
TGAAAACTACGAGGCCGATGATATTATCGGCACCCTTGCCAAATCAGCGGAAAAAGACGGATTTGAAGTGAAGATTTTTT
CCGGCGACAAGGACCTGACACAGCTGGCTACAGAAGGAACGACGGTCGCCATCACAAAAAAGGGCATTACAGATGTCGAA
TACTATACGCCTGAACATGTCCGGGAAAAGTACGGACTTACCCCTGAACAGATCATTGATATGAAAGGGCTGATGGGAGA
CTCGTCAGACAATATTCCAGGCGTGCCGGGAGTCGGCGAAAAGACGGCGATCAAGCTTTTGAAACAGTTCCATACAGTAG
AGGAGCTGCTGTCGTCAATAGACGAAGTCAGCGGAAAAAAACTGAAAGAAAAGCTTGAAGAATTTAAAGAACAGGCTTTG
ATGAGCAAAGAGCTTGCGACGATTACGACAGAAGCGCCGCTTGAAGTCAGCCTCGATTCCCTCGGATATGAAGGTTTTGA
CAGGGAAGCGGTCGTCAAGATTTTTAAAGATCTCGGCTTTCATTCTTTGCTCGAAAGGATTGGCGAAGAGGCGGGAGAGA
AAGAAGAAGAACAGTTGGAAGAGATCGATGTCATGATCAAAACAGACATCACGGATGACTTGTTTGCGTCACCGGCTTCA
CTCGTAGTCGAACAGCTCGGCGACAATTATCACGAGGCGCCGATCCTCGGTTTTTCGATCGTCAACGAACACGGCGCTTT
CTTCATTCCGGAAGAGACGGCCGTTCAGTCGGACTGTTTTAAAGAATGGGCCGAAGATGAGTCGAAAAAGAAATGGGTGT
TTGATGCAAAACGTGCGGCGGTCGCTTTGCGCTGGCGGGGCATTGAACTGAAAGGCGCTGAGTTTGACGTTCTTCTTGCG
GCCTATATCATCAACCCGGGGCACTCCTACGATGATGTGGCAAGCGTTGCGAAAGAACATCAATTACACATCGTTTCAGC
AGATGAAGCGGTCTACGGAAAAGGAGCGAAACAGGCGGTTCCGGATGAGAAGGAGCTTGCCGACCATTTAGCCAGAAAGG
CGAAGGCGATATCGCTGCTGCGCGAAAAGCTCCTTGATGAACTGGAAGAAAACGAGCAGCTCGAACTGTTTGAAGCGCTC
GAAATGCCCCTCGCCCACATTCTCGGTGAGATGGAATCGATCGGTGTCCAAGTCGATGTCGACAGACTGAAAAAAATGGG
TGAAGAACTGTCCGCTAAATTAGCGGAATACGAGAAAAAAATCCACGAGTCAGCAGGTGAAACCTTCAACATTAATTCAC
CAAAGCAGCTCGGTGTGATTTTGTTTGATAAACTCGGGCTGCCTGTCGTCAAAAAAACAAAAACGGGCTATTCAACTTCA
GCCGATGTGCTTGAAAAGCTGCGCGATAAACATGTCATTATCGAAGATATCCTGCACTACAGGCAGATTGGCAAGCTCCA
GTCCACATACGTAGAAGGGCTTTTGAAAGTGATCAAAAAAGACAGCCATAAAGTGCATACCCGCTTTAATCAAGCTTTAA
CACAGACGGGAAGGCTCAGCTCAACCGATCCGAATCTGCAAAATATACCGATCCGCCTTGAGGAGGGCCGCAAAATCCGG
CAGGCGTTTGTCCCTTCGCAAAAAGGCTGGCTCATATTTGCGGCGGATTACTCACAGATCGAGCTGAGGGTTCTCGCCCA
CATTTCAAAGGATAAAAATTTGATTGAAGCGTTCACGAACGATATGGACGTTCACACAAAAACGGCGATGGACGTTTTTC
ATGTATCAGAAGAAGAAGTCACCCCTGCGATGAGAAGGCAGGCGAAAGCAGTCAATTTCGGAATCGTCTACGGAATCAGC
GACTACGGCTTGTCGCAAAACCTGGGAATTACAAGAAAGGAAGCGGCAGCGTTTATTGAGCGCTATTTCCACAGCTTCCA
GGGAGTCAAAGAATACATGGAAGAGACCGTTCAGGAAGCGAAGCAGCGAGGCTATGTAACGACTCTTTTAAGCCGGAGGC
GGTATATACCGGAATTGACGAGCCGGAATTTCAATCTGCGCAGCTTTGCCGAACGGACGGCGATGAACACACCGATCCAG
GGAAGCGCCGCAGATATTATTAAAAAAGCGATGATCGACATGGCGGACAAGCTGAAAGAAAAAAATCTCCAGGCCAAACT
GCTGCTGCAGGTTCATGACGAATTGATTTTTGAAGCGCCTGAAGATGAAATCAAAGTGCTTGAAAAACTGGTTCCGGAAG
TCATGGAACATGCATTGGAGCTCGATGTTCCACTGAAAGTGGACTGCGCTTCAGGGCCTTCATGGTACGATGCAAAATAG

Upstream 100 bases:

>100_bases
CTCATTTTCCTTTCAAAATGAACTTTGCTAAAATAAAATAAGCAAATATGATCACGAAGTTGAATGGAAGCTGACGAATC
GGCTCCTTAGGAGGATAAAG

Downstream 100 bases:

>100_bases
AGAAAAGGAAGTGATGATGTGCCGGAATTACCAGAAGTCGAAACGGTCCGGCGCACTCTTGCCGGGCTTGTAAGAGGGAA
AACGATCGATGCGGTTGATG

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 879; Mature: 878

Protein sequence:

>879_residues
MTERKKLVLVDGNSLAYRAFFALPLLSNEKGIHTNAVYGFTTILMKMLEEEKPTHMLVAFDAGKTTFRHKTFKEYKGGRQ
KTPPELSEQLPFIRELLDAYRISRYELENYEADDIIGTLAKSAEKDGFEVKIFSGDKDLTQLATEGTTVAITKKGITDVE
YYTPEHVREKYGLTPEQIIDMKGLMGDSSDNIPGVPGVGEKTAIKLLKQFHTVEELLSSIDEVSGKKLKEKLEEFKEQAL
MSKELATITTEAPLEVSLDSLGYEGFDREAVVKIFKDLGFHSLLERIGEEAGEKEEEQLEEIDVMIKTDITDDLFASPAS
LVVEQLGDNYHEAPILGFSIVNEHGAFFIPEETAVQSDCFKEWAEDESKKKWVFDAKRAAVALRWRGIELKGAEFDVLLA
AYIINPGHSYDDVASVAKEHQLHIVSADEAVYGKGAKQAVPDEKELADHLARKAKAISLLREKLLDELEENEQLELFEAL
EMPLAHILGEMESIGVQVDVDRLKKMGEELSAKLAEYEKKIHESAGETFNINSPKQLGVILFDKLGLPVVKKTKTGYSTS
ADVLEKLRDKHVIIEDILHYRQIGKLQSTYVEGLLKVIKKDSHKVHTRFNQALTQTGRLSSTDPNLQNIPIRLEEGRKIR
QAFVPSQKGWLIFAADYSQIELRVLAHISKDKNLIEAFTNDMDVHTKTAMDVFHVSEEEVTPAMRRQAKAVNFGIVYGIS
DYGLSQNLGITRKEAAAFIERYFHSFQGVKEYMEETVQEAKQRGYVTTLLSRRRYIPELTSRNFNLRSFAERTAMNTPIQ
GSAADIIKKAMIDMADKLKEKNLQAKLLLQVHDELIFEAPEDEIKVLEKLVPEVMEHALELDVPLKVDCASGPSWYDAK

Sequences:

>Translated_879_residues
MTERKKLVLVDGNSLAYRAFFALPLLSNEKGIHTNAVYGFTTILMKMLEEEKPTHMLVAFDAGKTTFRHKTFKEYKGGRQ
KTPPELSEQLPFIRELLDAYRISRYELENYEADDIIGTLAKSAEKDGFEVKIFSGDKDLTQLATEGTTVAITKKGITDVE
YYTPEHVREKYGLTPEQIIDMKGLMGDSSDNIPGVPGVGEKTAIKLLKQFHTVEELLSSIDEVSGKKLKEKLEEFKEQAL
MSKELATITTEAPLEVSLDSLGYEGFDREAVVKIFKDLGFHSLLERIGEEAGEKEEEQLEEIDVMIKTDITDDLFASPAS
LVVEQLGDNYHEAPILGFSIVNEHGAFFIPEETAVQSDCFKEWAEDESKKKWVFDAKRAAVALRWRGIELKGAEFDVLLA
AYIINPGHSYDDVASVAKEHQLHIVSADEAVYGKGAKQAVPDEKELADHLARKAKAISLLREKLLDELEENEQLELFEAL
EMPLAHILGEMESIGVQVDVDRLKKMGEELSAKLAEYEKKIHESAGETFNINSPKQLGVILFDKLGLPVVKKTKTGYSTS
ADVLEKLRDKHVIIEDILHYRQIGKLQSTYVEGLLKVIKKDSHKVHTRFNQALTQTGRLSSTDPNLQNIPIRLEEGRKIR
QAFVPSQKGWLIFAADYSQIELRVLAHISKDKNLIEAFTNDMDVHTKTAMDVFHVSEEEVTPAMRRQAKAVNFGIVYGIS
DYGLSQNLGITRKEAAAFIERYFHSFQGVKEYMEETVQEAKQRGYVTTLLSRRRYIPELTSRNFNLRSFAERTAMNTPIQ
GSAADIIKKAMIDMADKLKEKNLQAKLLLQVHDELIFEAPEDEIKVLEKLVPEVMEHALELDVPLKVDCASGPSWYDAK
>Mature_878_residues
TERKKLVLVDGNSLAYRAFFALPLLSNEKGIHTNAVYGFTTILMKMLEEEKPTHMLVAFDAGKTTFRHKTFKEYKGGRQK
TPPELSEQLPFIRELLDAYRISRYELENYEADDIIGTLAKSAEKDGFEVKIFSGDKDLTQLATEGTTVAITKKGITDVEY
YTPEHVREKYGLTPEQIIDMKGLMGDSSDNIPGVPGVGEKTAIKLLKQFHTVEELLSSIDEVSGKKLKEKLEEFKEQALM
SKELATITTEAPLEVSLDSLGYEGFDREAVVKIFKDLGFHSLLERIGEEAGEKEEEQLEEIDVMIKTDITDDLFASPASL
VVEQLGDNYHEAPILGFSIVNEHGAFFIPEETAVQSDCFKEWAEDESKKKWVFDAKRAAVALRWRGIELKGAEFDVLLAA
YIINPGHSYDDVASVAKEHQLHIVSADEAVYGKGAKQAVPDEKELADHLARKAKAISLLREKLLDELEENEQLELFEALE
MPLAHILGEMESIGVQVDVDRLKKMGEELSAKLAEYEKKIHESAGETFNINSPKQLGVILFDKLGLPVVKKTKTGYSTSA
DVLEKLRDKHVIIEDILHYRQIGKLQSTYVEGLLKVIKKDSHKVHTRFNQALTQTGRLSSTDPNLQNIPIRLEEGRKIRQ
AFVPSQKGWLIFAADYSQIELRVLAHISKDKNLIEAFTNDMDVHTKTAMDVFHVSEEEVTPAMRRQAKAVNFGIVYGISD
YGLSQNLGITRKEAAAFIERYFHSFQGVKEYMEETVQEAKQRGYVTTLLSRRRYIPELTSRNFNLRSFAERTAMNTPIQG
SAADIIKKAMIDMADKLKEKNLQAKLLLQVHDELIFEAPEDEIKVLEKLVPEVMEHALELDVPLKVDCASGPSWYDAK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=516, Percent_Identity=35.4651162790698, Blast_Score=266, Evalue=5e-71,
Organism=Homo sapiens, GI139394648, Length=609, Percent_Identity=27.0935960591133, Blast_Score=208, Evalue=1e-53,
Organism=Escherichia coli, GI1790294, Length=928, Percent_Identity=39.5474137931034, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI226510970, Length=221, Percent_Identity=29.8642533936652, Blast_Score=116, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI86563272, Length=421, Percent_Identity=31.8289786223278, Blast_Score=187, Evalue=3e-47,
Organism=Drosophila melanogaster, GI17933644, Length=429, Percent_Identity=33.1002331002331, Blast_Score=234, Evalue=1e-61,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 99211; Mature: 99080

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERKKLVLVDGNSLAYRAFFALPLLSNEKGIHTNAVYGFTTILMKMLEEEKPTHMLVAF
CCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
DAGKTTFRHKTFKEYKGGRQKTPPELSEQLPFIRELLDAYRISRYELENYEADDIIGTLA
ECCCCHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KSAEKDGFEVKIFSGDKDLTQLATEGTTVAITKKGITDVEYYTPEHVREKYGLTPEQIID
HHCCCCCCEEEEECCCHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHH
MKGLMGDSSDNIPGVPGVGEKTAIKLLKQFHTVEELLSSIDEVSGKKLKEKLEEFKEQAL
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
MSKELATITTEAPLEVSLDSLGYEGFDREAVVKIFKDLGFHSLLERIGEEAGEKEEEQLE
HHHHHHHHCCCCCCEEEHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHH
EIDVMIKTDITDDLFASPASLVVEQLGDNYHEAPILGFSIVNEHGAFFIPEETAVQSDCF
HHHHHEEECCCHHHHCCHHHHHHHHHCCCCCCCCEEEHEEECCCCCEECCCHHHHHHHHH
KEWAEDESKKKWVFDAKRAAVALRWRGIELKGAEFDVLLAAYIINPGHSYDDVASVAKEH
HHHHCCCCCCCHHHHHHHHEEEEEEECEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHC
QLHIVSADEAVYGKGAKQAVPDEKELADHLARKAKAISLLREKLLDELEENEQLELFEAL
CEEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
EMPLAHILGEMESIGVQVDVDRLKKMGEELSAKLAEYEKKIHESAGETFNINSPKQLGVI
HHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHCHH
LFDKLGLPVVKKTKTGYSTSADVLEKLRDKHVIIEDILHYRQIGKLQSTYVEGLLKVIKK
EECCCCCCEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DSHKVHTRFNQALTQTGRLSSTDPNLQNIPIRLEEGRKIRQAFVPSQKGWLIFAADYSQI
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCEEEEEECCCHH
ELRVLAHISKDKNLIEAFTNDMDVHTKTAMDVFHVSEEEVTPAMRRQAKAVNFGIVYGIS
HHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCHHHHHHHHHCCEEEEEEEC
DYGLSQNLGITRKEAAAFIERYFHSFQGVKEYMEETVQEAKQRGYVTTLLSRRRYIPELT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHH
SRNFNLRSFAERTAMNTPIQGSAADIIKKAMIDMADKLKEKNLQAKLLLQVHDELIFEAP
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
EDEIKVLEKLVPEVMEHALELDVPLKVDCASGPSWYDAK
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
TERKKLVLVDGNSLAYRAFFALPLLSNEKGIHTNAVYGFTTILMKMLEEEKPTHMLVAF
CCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
DAGKTTFRHKTFKEYKGGRQKTPPELSEQLPFIRELLDAYRISRYELENYEADDIIGTLA
ECCCCHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KSAEKDGFEVKIFSGDKDLTQLATEGTTVAITKKGITDVEYYTPEHVREKYGLTPEQIID
HHCCCCCCEEEEECCCHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHH
MKGLMGDSSDNIPGVPGVGEKTAIKLLKQFHTVEELLSSIDEVSGKKLKEKLEEFKEQAL
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
MSKELATITTEAPLEVSLDSLGYEGFDREAVVKIFKDLGFHSLLERIGEEAGEKEEEQLE
HHHHHHHHCCCCCCEEEHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHH
EIDVMIKTDITDDLFASPASLVVEQLGDNYHEAPILGFSIVNEHGAFFIPEETAVQSDCF
HHHHHEEECCCHHHHCCHHHHHHHHHCCCCCCCCEEEHEEECCCCCEECCCHHHHHHHHH
KEWAEDESKKKWVFDAKRAAVALRWRGIELKGAEFDVLLAAYIINPGHSYDDVASVAKEH
HHHHCCCCCCCHHHHHHHHEEEEEEECEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHC
QLHIVSADEAVYGKGAKQAVPDEKELADHLARKAKAISLLREKLLDELEENEQLELFEAL
CEEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
EMPLAHILGEMESIGVQVDVDRLKKMGEELSAKLAEYEKKIHESAGETFNINSPKQLGVI
HHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHCHH
LFDKLGLPVVKKTKTGYSTSADVLEKLRDKHVIIEDILHYRQIGKLQSTYVEGLLKVIKK
EECCCCCCEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DSHKVHTRFNQALTQTGRLSSTDPNLQNIPIRLEEGRKIRQAFVPSQKGWLIFAADYSQI
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCEEEEEECCCHH
ELRVLAHISKDKNLIEAFTNDMDVHTKTAMDVFHVSEEEVTPAMRRQAKAVNFGIVYGIS
HHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCHHHHHHHHHCCEEEEEEEC
DYGLSQNLGITRKEAAAFIERYFHSFQGVKEYMEETVQEAKQRGYVTTLLSRRRYIPELT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHH
SRNFNLRSFAERTAMNTPIQGSAADIIKKAMIDMADKLKEKNLQAKLLLQVHDELIFEAP
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
EDEIKVLEKLVPEVMEHALELDVPLKVDCASGPSWYDAK
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]