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The map label for this gene is mutM

Identifier: 52786785

GI number: 52786785

Start: 2939475

End: 2940302

Strand: Reverse

Name: mutM

Synonym: BLi03056

Alternate gene names: 52786785

Gene position: 2940302-2939475 (Counterclockwise)

Preceding gene: 52786786

Following gene: 52786784

Centisome position: 69.63

GC content: 49.15

Gene sequence:

>828_bases
GTGCCGGAATTACCAGAAGTCGAAACGGTCCGGCGCACTCTTGCCGGGCTTGTAAGAGGGAAAACGATCGATGCGGTTGA
TGTCAGATGGACAAAAATCATAAAACGTCCGGAGGAGCCTGAGGAATTCGCAAGGCTCCTGGCCGGCCAGACGATTCAGT
CGATCGGAAGAAGGGGAAAATTCCTGTTGTTTCATCTTGATGATTGTGTCATGGTTTCCCATTTGAGAATGGAAGGAAAG
TACGGGCTTCATCAAAACGATGAACCCCTTGATAAACATGTGCACGTCATTTTCAGGTTCACGGACGGAAGCGAGCTGCG
CTACCGGGATGTCAGAAAGTTCGGCACCATGCATTTGTTTAAGCCGGGAGAGGAACTGACAGAGCTGCCTCTCAGGCAGC
TCGGCCCCGAGCCCTTCAGCAGCGAATTTACAGCCGATTACTTAAGAGAGCGCCTGAAGAAAACAAACCGCTCGGTGAAA
ACCGCGCTGCTCGATCAGCGGACAGTCGTCGGACTGGGCAATATTTATGTCGACGAGGCTTTGTTCAGAGCCGGTATCCA
TCCCGAGGCAACAGCGAACAAGCTCACAAAAAAACAGACGGTTCTTCTCCATAAAGAGATCATTCAAACGTTAAAGGAAG
CTGTTGAAGCAGGGGGAAGCACAGTCAGATCTTACATCAATTCACAAGGTGAGATCGGCATGTTCCAGCTCAAACTTTTC
GTTTACGGCAGGAAAGATGAACCTTGTAAAAAATGCGGATCTCCAATTGAAAAAACAGTCGTTGGCGGAAGGGGCACGCA
TTTTTGCATAAAGTGCCAGAAAAAATGA

Upstream 100 bases:

>100_bases
AGTCATGGAACATGCATTGGAGCTCGATGTTCCACTGAAAGTGGACTGCGCTTCAGGGCCTTCATGGTACGATGCAAAAT
AGAGAAAAGGAAGTGATGAT

Downstream 100 bases:

>100_bases
CAATCGCATAGAAGGACAAAAGCAAGCCGTATCATGGTTCAAGCTGTCGGTGCATACGCTCCATATAGCTGTTAAAAGCA
AGCTTTAAGGTGTGATGTTT

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MPELPEVETVRRTLAGLVRGKTIDAVDVRWTKIIKRPEEPEEFARLLAGQTIQSIGRRGKFLLFHLDDCVMVSHLRMEGK
YGLHQNDEPLDKHVHVIFRFTDGSELRYRDVRKFGTMHLFKPGEELTELPLRQLGPEPFSSEFTADYLRERLKKTNRSVK
TALLDQRTVVGLGNIYVDEALFRAGIHPEATANKLTKKQTVLLHKEIIQTLKEAVEAGGSTVRSYINSQGEIGMFQLKLF
VYGRKDEPCKKCGSPIEKTVVGGRGTHFCIKCQKK

Sequences:

>Translated_275_residues
MPELPEVETVRRTLAGLVRGKTIDAVDVRWTKIIKRPEEPEEFARLLAGQTIQSIGRRGKFLLFHLDDCVMVSHLRMEGK
YGLHQNDEPLDKHVHVIFRFTDGSELRYRDVRKFGTMHLFKPGEELTELPLRQLGPEPFSSEFTADYLRERLKKTNRSVK
TALLDQRTVVGLGNIYVDEALFRAGIHPEATANKLTKKQTVLLHKEIIQTLKEAVEAGGSTVRSYINSQGEIGMFQLKLF
VYGRKDEPCKKCGSPIEKTVVGGRGTHFCIKCQKK
>Mature_274_residues
PELPEVETVRRTLAGLVRGKTIDAVDVRWTKIIKRPEEPEEFARLLAGQTIQSIGRRGKFLLFHLDDCVMVSHLRMEGKY
GLHQNDEPLDKHVHVIFRFTDGSELRYRDVRKFGTMHLFKPGEELTELPLRQLGPEPFSSEFTADYLRERLKKTNRSVKT
ALLDQRTVVGLGNIYVDEALFRAGIHPEATANKLTKKQTVLLHKEIIQTLKEAVEAGGSTVRSYINSQGEIGMFQLKLFV
YGRKDEPCKKCGSPIEKTVVGGRGTHFCIKCQKK

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=274, Percent_Identity=40.5109489051095, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1786932, Length=281, Percent_Identity=27.7580071174377, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_BACLD (Q65G93)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080200.1
- RefSeq:   YP_092614.1
- ProteinModelPortal:   Q65G93
- SMR:   Q65G93
- STRING:   Q65G93
- EnsemblBacteria:   EBBACT00000054830
- EnsemblBacteria:   EBBACT00000061130
- GeneID:   3027638
- GeneID:   3098654
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03056
- KEGG:   bli:BL00393
- NMPDR:   fig|279010.5.peg.3419
- eggNOG:   COG0266
- GeneTree:   EBGT00050000000934
- HOGENOM:   HBG690070
- OMA:   RMTGQLL
- ProtClustDB:   PRK01103
- BioCyc:   BLIC279010-1:BLI03056-MONOMER
- BioCyc:   BLIC279010:BL00393-MONOMER
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 31318; Mature: 31187

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 60-60 ACT_SITE 264-264 BINDING 93-93 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETVRRTLAGLVRGKTIDAVDVRWTKIIKRPEEPEEFARLLAGQTIQSIGRRGK
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
FLLFHLDDCVMVSHLRMEGKYGLHQNDEPLDKHVHVIFRFTDGSELRYRDVRKFGTMHLF
EEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCEEEE
KPGEELTELPLRQLGPEPFSSEFTADYLRERLKKTNRSVKTALLDQRTVVGLGNIYVDEA
CCCHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHHHHH
LFRAGIHPEATANKLTKKQTVLLHKEIIQTLKEAVEAGGSTVRSYINSQGEIGMFQLKLF
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEEEE
VYGRKDEPCKKCGSPIEKTVVGGRGTHFCIKCQKK
EECCCCCHHHHHCCCHHHHHCCCCCCEEEEEECCC
>Mature Secondary Structure 
PELPEVETVRRTLAGLVRGKTIDAVDVRWTKIIKRPEEPEEFARLLAGQTIQSIGRRGK
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
FLLFHLDDCVMVSHLRMEGKYGLHQNDEPLDKHVHVIFRFTDGSELRYRDVRKFGTMHLF
EEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCEEEE
KPGEELTELPLRQLGPEPFSSEFTADYLRERLKKTNRSVKTALLDQRTVVGLGNIYVDEA
CCCHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHHHHH
LFRAGIHPEATANKLTKKQTVLLHKEIIQTLKEAVEAGGSTVRSYINSQGEIGMFQLKLF
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEEEE
VYGRKDEPCKKCGSPIEKTVVGGRGTHFCIKCQKK
EECCCCCHHHHHCCCHHHHHCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA