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The map label for this gene is folD

Identifier: 52786340

GI number: 52786340

Start: 2522353

End: 2523207

Strand: Reverse

Name: folD

Synonym: BLi02602

Alternate gene names: 52786340

Gene position: 2523207-2522353 (Counterclockwise)

Preceding gene: 52786341

Following gene: 52786339

Centisome position: 59.75

GC content: 48.19

Gene sequence:

>855_bases
ATGACAGCAACCATCATCGACGGGAAAGAAACAGCAAAGGAAAAGCGCGGGCAGCTGGCCAAGGAAGTTGAAGAGCTGAA
AAAACAAGGAGTTACCCCGGGACTGGCCGTTATTTTAATTGGTGATGATCCGGCGTCTCTTTCATATGTACGGGGAAAGA
AAAAAGCAGCTGAAGCAATGGGGATTCGTTTTCAGCTGGATCATTTCGATGCAAGCTTCACTGAACAGGAGCTTCTTGAA
GTGATTGACCAGTACAATCAAAATGACGACTTTCACGGAATTCTTGTCCAGCTGCCTCTTCCGGATCATATTTCCGAACA
AGCGGTGATCGAGAGAATATCCCCTGAGAAAGATGTTGACGGTTTTCATCCATTAAACGTAGGGAAGATGCTGCTTGGCG
AAGATACGTTTCTGCCTTGCACGCCTGCGGGGATCGTGGAGCTTTTAAAGAAAACCGAGATTGACCTGTCGGGAAAAGAG
GTTGTTGTCGTCGGACGAAGCAATATCGTCGGAAAGCCGGTAGGGCAGCTGCTCTTGAACGAAAACGCAACCGTTACGTA
CTGCCATTCAAGAACGGCCGACATTACGGCTCATACAAAAAAAGCCGACATTCTGATTGTCGCTGTCGGAAAGGCGAATT
TCATCAAAGCGGATCAAATTAAAGAGGGAGCCGTCGTCATCGATGTCGGCGTCAACCGCCTTGACAACGGAAAGCTCGCC
GGTGATGTCGATTTTGAAGAAGCGAAGGAAAAGGCTTCCTATATTACACCTGTACCGGGCGGTGTCGGTCCGATGACGAT
TACAATGCTGGCCCACAACACGGTTAAATCAGCGAAGCGTACAATTCAACTTTAA

Upstream 100 bases:

>100_bases
GAACTGGCAAAGCAATTTGGTGATGACAAGTCGGCGAAGTTTGTAAACGGTGTTCTTTCTAACATTAAATCCGATCTTGA
ATAATCTAGGAGGAGAGCAA

Downstream 100 bases:

>100_bases
TGTACAGCCGTTCATCCGCCTGGTCTCGCGCCGGCGGATGAACGTTCTTGTGCCTTCTTAGAAAGGAGACTGTGCCATGA
GTGAAATATCATTTGTCACG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTATIIDGKETAKEKRGQLAKEVEELKKQGVTPGLAVILIGDDPASLSYVRGKKKAAEAMGIRFQLDHFDASFTEQELLE
VIDQYNQNDDFHGILVQLPLPDHISEQAVIERISPEKDVDGFHPLNVGKMLLGEDTFLPCTPAGIVELLKKTEIDLSGKE
VVVVGRSNIVGKPVGQLLLNENATVTYCHSRTADITAHTKKADILIVAVGKANFIKADQIKEGAVVIDVGVNRLDNGKLA
GDVDFEEAKEKASYITPVPGGVGPMTITMLAHNTVKSAKRTIQL

Sequences:

>Translated_284_residues
MTATIIDGKETAKEKRGQLAKEVEELKKQGVTPGLAVILIGDDPASLSYVRGKKKAAEAMGIRFQLDHFDASFTEQELLE
VIDQYNQNDDFHGILVQLPLPDHISEQAVIERISPEKDVDGFHPLNVGKMLLGEDTFLPCTPAGIVELLKKTEIDLSGKE
VVVVGRSNIVGKPVGQLLLNENATVTYCHSRTADITAHTKKADILIVAVGKANFIKADQIKEGAVVIDVGVNRLDNGKLA
GDVDFEEAKEKASYITPVPGGVGPMTITMLAHNTVKSAKRTIQL
>Mature_283_residues
TATIIDGKETAKEKRGQLAKEVEELKKQGVTPGLAVILIGDDPASLSYVRGKKKAAEAMGIRFQLDHFDASFTEQELLEV
IDQYNQNDDFHGILVQLPLPDHISEQAVIERISPEKDVDGFHPLNVGKMLLGEDTFLPCTPAGIVELLKKTEIDLSGKEV
VVVGRSNIVGKPVGQLLLNENATVTYCHSRTADITAHTKKADILIVAVGKANFIKADQIKEGAVVIDVGVNRLDNGKLAG
DVDFEEAKEKASYITPVPGGVGPMTITMLAHNTVKSAKRTIQL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=293, Percent_Identity=47.098976109215, Blast_Score=254, Evalue=5e-68,
Organism=Homo sapiens, GI94721354, Length=298, Percent_Identity=47.6510067114094, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI222418558, Length=296, Percent_Identity=46.2837837837838, Blast_Score=247, Evalue=7e-66,
Organism=Homo sapiens, GI36796743, Length=217, Percent_Identity=25.8064516129032, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=51.2544802867383, Blast_Score=278, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=41.4383561643836, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6321643, Length=301, Percent_Identity=44.8504983388704, Blast_Score=256, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=45.8041958041958, Blast_Score=248, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6322933, Length=329, Percent_Identity=30.0911854103343, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=44.2176870748299, Blast_Score=240, Evalue=6e-64,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=44.2176870748299, Blast_Score=240, Evalue=6e-64,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=44.5205479452055, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=44.5205479452055, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=44.5205479452055, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=44.5205479452055, Blast_Score=238, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_BACLD (Q65HI8)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079755.1
- RefSeq:   YP_092169.1
- HSSP:   P11586
- ProteinModelPortal:   Q65HI8
- SMR:   Q65HI8
- STRING:   Q65HI8
- EnsemblBacteria:   EBBACT00000055508
- EnsemblBacteria:   EBBACT00000062621
- GeneID:   3028426
- GeneID:   3100912
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02602
- KEGG:   bli:BL01527
- NMPDR:   fig|279010.5.peg.447
- eggNOG:   COG0190
- GeneTree:   EBGT00050000002737
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- ProtClustDB:   PRK14190
- BioCyc:   BLIC279010-1:BLI02602-MONOMER
- BioCyc:   BLIC279010:BL01527-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30728; Mature: 30597

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATIIDGKETAKEKRGQLAKEVEELKKQGVTPGLAVILIGDDPASLSYVRGKKKAAEAM
CEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHCHHHHHHHH
GIRFQLDHFDASFTEQELLEVIDQYNQNDDFHGILVQLPLPDHISEQAVIERISPEKDVD
CEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCC
GFHPLNVGKMLLGEDTFLPCTPAGIVELLKKTEIDLSGKEVVVVGRSNIVGKPVGQLLLN
CCCCCCCCHHEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHCC
ENATVTYCHSRTADITAHTKKADILIVAVGKANFIKADQIKEGAVVIDVGVNRLDNGKLA
CCCEEEEEECCCCCEEEECCCCCEEEEEECCCCCEECCCCCCCCEEEEECCCCCCCCCEE
GDVDFEEAKEKASYITPVPGGVGPMTITMLAHNTVKSAKRTIQL
CCCCHHHHHHHCCEECCCCCCCCCEEEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
TATIIDGKETAKEKRGQLAKEVEELKKQGVTPGLAVILIGDDPASLSYVRGKKKAAEAM
EEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHCHHHHHHHH
GIRFQLDHFDASFTEQELLEVIDQYNQNDDFHGILVQLPLPDHISEQAVIERISPEKDVD
CEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCC
GFHPLNVGKMLLGEDTFLPCTPAGIVELLKKTEIDLSGKEVVVVGRSNIVGKPVGQLLLN
CCCCCCCCHHEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHCC
ENATVTYCHSRTADITAHTKKADILIVAVGKANFIKADQIKEGAVVIDVGVNRLDNGKLA
CCCEEEEEECCCCCEEEECCCCCEEEEEECCCCCEECCCCCCCCEEEEECCCCCCCCCEE
GDVDFEEAKEKASYITPVPGGVGPMTITMLAHNTVKSAKRTIQL
CCCCHHHHHHHCCEECCCCCCCCCEEEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA